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The map label for this gene is ackA

Identifier: 157371553

GI number: 157371553

Start: 3672043

End: 3673245

Strand: Direct

Name: ackA

Synonym: Spro_3316

Alternate gene names: 157371553

Gene position: 3672043-3673245 (Clockwise)

Preceding gene: 157371548

Following gene: 157371554

Centisome position: 67.39

GC content: 57.36

Gene sequence:

>1203_bases
ATGTCGAGTAAGCTAGTACTGGTCCTGAACTGCGGCAGTTCTTCCCTGAAGTTCGCTATCATCGATGCTGTCAACGGTGA
AGAACACCTCTCCGGCCTGGCCGAATGTTTCCACCTGCCTGAGGCTCGCCTCAAATGGAAGATGGACGGCGCCAAACACG
AAGCGGCTCTGGGTGCCGGCGCTGCGCACAGCGAAGCACTGAACTACATTGTTAATACTATTCTGGCACAAAAACCGGAG
CTTTCTGCACAGCTGACCGCTATCGGTCACCGCATTGTGCACGGCGGCGAGAAGTTTACCGCCTCTGCCGTGATCAACGA
CGAAGTTCTGCAGGGTATCAAAGATTCGGTGCCTTTTGCACCACTGCATAACCCGGCGCACCTGATCGGTATCGCTGAAG
CATTGAAATCCTTCCCTAAACTGGCGGATAAAAACGTTGCCGTGTTCGACACCGCATTCCACCAGACCATGCCTGAAGAA
TCCTACCTGTACGCCCTGCCGTACAGCCTGTACCGCGACCACAGCGTTCGTCGCTATGGCGCACACGGCACCAGCCACTT
CTATGTAACCCAGGAAGCGGCCAAGGCCCTGAACAAGCCGGTTGAAGAAGTCAACCTGATCACCTGCCACCTGGGCAACG
GCGGTTCCGTTACCGCAGTGCGCAACGGCAAATGCGTTGACACCTCCATGGGTCTGACCCCACTGGAAGGTCTGGTCATG
GGCACCCGCAGCGGTGACATCGATCCGGCCATCATCTTCCACCTGCACGACTCCCTGGGCATGAGCGTTGATCAAATCAA
CAAAATGCTGACCAAAGAGTCCGGCCTGCTGGGCCTGACCGAAGTCACCAGCGACTGCCGTTATGTTGAAGACAACTACG
AAAGCAAAGCCGACGCCAAGCGTGCGATGGACGTATTCTGCCACCGCCTGGCCAAATACATCGGTGCCTACAGCGCACTG
ATGGACGGCCGTCTGGACGCGGTGATCTTCACCGGCGGTATCGGTGAAAACGCCGGTATGGTGCGTGAGCTGACCCTGAA
CAAACTGGGCCTGCTGGGCTTCGAGATTGACCACGAGCGCAACATGGCTGCTCGCTTCGGTAAATCCGGTGCCATCACCA
AAGACGGCAGCCGCCTGGCGCTGGTAATCCCGACCAACGAAGAGTTGGTCATCGCGCAAGACGCATCCCGTCTGACCGCG
TAA

Upstream 100 bases:

>100_bases
AACGGCATTATGCGCTACGCTTGTTGTCTGATTGACGATTAATTCACCACGTGTTTTGGTCTTTCCTTTAACAACACGAA
CTATTTAAGTAGGTACTTCC

Downstream 100 bases:

>100_bases
CGCTCTCCGACACCGTCAGCCCAGGCTGACGGTGCTGTTTTAGCCTCATGTTAAATCGTGAAGAGGACTATTCTGTGTCA
CGTACTATTATGTTGATCCC

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MSSKLVLVLNCGSSSLKFAIIDAVNGEEHLSGLAECFHLPEARLKWKMDGAKHEAALGAGAAHSEALNYIVNTILAQKPE
LSAQLTAIGHRIVHGGEKFTASAVINDEVLQGIKDSVPFAPLHNPAHLIGIAEALKSFPKLADKNVAVFDTAFHQTMPEE
SYLYALPYSLYRDHSVRRYGAHGTSHFYVTQEAAKALNKPVEEVNLITCHLGNGGSVTAVRNGKCVDTSMGLTPLEGLVM
GTRSGDIDPAIIFHLHDSLGMSVDQINKMLTKESGLLGLTEVTSDCRYVEDNYESKADAKRAMDVFCHRLAKYIGAYSAL
MDGRLDAVIFTGGIGENAGMVRELTLNKLGLLGFEIDHERNMAARFGKSGAITKDGSRLALVIPTNEELVIAQDASRLTA

Sequences:

>Translated_400_residues
MSSKLVLVLNCGSSSLKFAIIDAVNGEEHLSGLAECFHLPEARLKWKMDGAKHEAALGAGAAHSEALNYIVNTILAQKPE
LSAQLTAIGHRIVHGGEKFTASAVINDEVLQGIKDSVPFAPLHNPAHLIGIAEALKSFPKLADKNVAVFDTAFHQTMPEE
SYLYALPYSLYRDHSVRRYGAHGTSHFYVTQEAAKALNKPVEEVNLITCHLGNGGSVTAVRNGKCVDTSMGLTPLEGLVM
GTRSGDIDPAIIFHLHDSLGMSVDQINKMLTKESGLLGLTEVTSDCRYVEDNYESKADAKRAMDVFCHRLAKYIGAYSAL
MDGRLDAVIFTGGIGENAGMVRELTLNKLGLLGFEIDHERNMAARFGKSGAITKDGSRLALVIPTNEELVIAQDASRLTA
>Mature_399_residues
SSKLVLVLNCGSSSLKFAIIDAVNGEEHLSGLAECFHLPEARLKWKMDGAKHEAALGAGAAHSEALNYIVNTILAQKPEL
SAQLTAIGHRIVHGGEKFTASAVINDEVLQGIKDSVPFAPLHNPAHLIGIAEALKSFPKLADKNVAVFDTAFHQTMPEES
YLYALPYSLYRDHSVRRYGAHGTSHFYVTQEAAKALNKPVEEVNLITCHLGNGGSVTAVRNGKCVDTSMGLTPLEGLVMG
TRSGDIDPAIIFHLHDSLGMSVDQINKMLTKESGLLGLTEVTSDCRYVEDNYESKADAKRAMDVFCHRLAKYIGAYSALM
DGRLDAVIFTGGIGENAGMVRELTLNKLGLLGFEIDHERNMAARFGKSGAITKDGSRLALVIPTNEELVIAQDASRLTA

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=400, Percent_Identity=87.75, Blast_Score=745, Evalue=0.0,
Organism=Escherichia coli, GI145693184, Length=396, Percent_Identity=45.4545454545455, Blast_Score=321, Evalue=5e-89,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_SERP5 (A8GH24)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479542.1
- ProteinModelPortal:   A8GH24
- SMR:   A8GH24
- STRING:   A8GH24
- GeneID:   5603182
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3316
- eggNOG:   COG0282
- HOGENOM:   HBG285863
- OMA:   PHLKDKN
- ProtClustDB:   PRK00180
- BioCyc:   SPRO399741:SPRO_3316-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 43129; Mature: 42998

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKLVLVLNCGSSSLKFAIIDAVNGEEHLSGLAECFHLPEARLKWKMDGAKHEAALGAG
CCCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHCCCHHEEEEECCCCHHHHHCCC
AAHSEALNYIVNTILAQKPELSAQLTAIGHRIVHGGEKFTASAVINDEVLQGIKDSVPFA
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHCCCCC
PLHNPAHLIGIAEALKSFPKLADKNVAVFDTAFHQTMPEESYLYALPYSLYRDHSVRRYG
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCEEEECCHHHHHCCCCHHHC
AHGTSHFYVTQEAAKALNKPVEEVNLITCHLGNGGSVTAVRNGKCVDTSMGLTPLEGLVM
CCCCEEEEEEHHHHHHHCCCHHHEEEEEEEECCCCCEEEEECCEEEECCCCCCCCCCEEE
GTRSGDIDPAIIFHLHDSLGMSVDQINKMLTKESGLLGLTEVTSDCRYVEDNYESKADAK
ECCCCCCCCEEEEEECHHHCCCHHHHHHHHHHCCCCEEHHHHHCCCHHHHCCCCCHHHHH
RAMDVFCHRLAKYIGAYSALMDGRLDAVIFTGGIGENAGMVRELTLNKLGLLGFEIDHER
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHCEEEEEECCCC
NMAARFGKSGAITKDGSRLALVIPTNEELVIAQDASRLTA
CHHHHCCCCCCEECCCCEEEEEEECCCCEEEEECCHHCCC
>Mature Secondary Structure 
SSKLVLVLNCGSSSLKFAIIDAVNGEEHLSGLAECFHLPEARLKWKMDGAKHEAALGAG
CCCEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHCCCHHEEEEECCCCHHHHHCCC
AAHSEALNYIVNTILAQKPELSAQLTAIGHRIVHGGEKFTASAVINDEVLQGIKDSVPFA
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHCCCCC
PLHNPAHLIGIAEALKSFPKLADKNVAVFDTAFHQTMPEESYLYALPYSLYRDHSVRRYG
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCEEEECCHHHHHCCCCHHHC
AHGTSHFYVTQEAAKALNKPVEEVNLITCHLGNGGSVTAVRNGKCVDTSMGLTPLEGLVM
CCCCEEEEEEHHHHHHHCCCHHHEEEEEEEECCCCCEEEEECCEEEECCCCCCCCCCEEE
GTRSGDIDPAIIFHLHDSLGMSVDQINKMLTKESGLLGLTEVTSDCRYVEDNYESKADAK
ECCCCCCCCEEEEEECHHHCCCHHHHHHHHHHCCCCEEHHHHHCCCHHHHCCCCCHHHHH
RAMDVFCHRLAKYIGAYSALMDGRLDAVIFTGGIGENAGMVRELTLNKLGLLGFEIDHER
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHCEEEEEECCCC
NMAARFGKSGAITKDGSRLALVIPTNEELVIAQDASRLTA
CHHHHCCCCCCEECCCCEEEEEEECCCCEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA