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The map label for this gene is citH [H]

Identifier: 157371418

GI number: 157371418

Start: 3500271

End: 3501566

Strand: Direct

Name: citH [H]

Synonym: Spro_3179

Alternate gene names: 157371418

Gene position: 3500271-3501566 (Clockwise)

Preceding gene: 157371417

Following gene: 157371419

Centisome position: 64.24

GC content: 55.32

Gene sequence:

>1296_bases
ATGCATTCCTCTAACGCCCCCATGACCCGCCGGGCCAGAATCAGCGCGATACTGCGAGTTACCTCTGGCAACTTTCTGGA
GCAGTTTGATTTTTTCCTGTTCGGGTTCTATGCCACCTACATCGCCCATACCTTTTTTCCGGCCAGCAGTGAATTTGCCT
CGCTGATGATGACCTTCGCGGTCTTTGGCGCGGGCTTTTTGATGCGTCCTATCGGTGCAATCGTGTTGGGTGCGTACATT
GATAAAGTCGGCAGACGTAAAGGGCTGATTGTTACCCTGTCGATCATGGCCGCCGGGACTTTCCTGATTGTACTCATTCC
GTCCTATCAGACGATCGGGATCTGGGCGCCGTTGCTGGTGCTGGGTGGCCGTTTATTGCAAGGCTTTTCAGCCGGTGCTG
AACTGGGTGGCGTATCCGTTTACCTGGCTGAAATTGCCACCCCTGGCCGTAAAGGCTTTTACACCAGTTGGCAGTCTGGC
AGCCAGCAAGTGGCCATTATGGTGGCAGCGGCGATGGGTTTCGCCCTGAATGCCGTCATGGAAGAAAGCGCCATTCGCGA
ATGGGGTTGGCGTCTGCCTTTCCTGTTCGGTTGCCTGATTGTCCCTTTCATCTTTTTCCTGCGCCGTAAACTGGAAGAGA
CCCAGGAGTTTAACGCCCGTCGCAACCACCTGGCGATGCGCGAGGTGTTTAAAACCCTCTTGGCAAACTGGCAGGTGGTT
ATTGCCGGCATGCTGATGGTCGCCATGACCACCACCGCGTTTTATTTGATCACCGTGTATGCACCGACGTTCGGTAAAAA
AGTGCTGATGCTAAGCGCCTCTGACAGCCTGCTGGTGACCTTGCTGGTAGCGATATCCAACTTCCTCTGGCTGCCGGTTG
GGGGCGCTCTGTCAGACCGTTTTGGTCGTAAACCGGTCCTGATTACCATGGCCCTGCTGGCACTGGTCACTGCGTATCCG
GCACTTGGCCTGCTTGCTGCAGCTCCCAGTTTCTCCATGATGCTCACGGTGCTGTTATGGCTCTCCTTCATCTATGGGCT
CTACAACGGTGCCATGATCCCGGCGTTAACCGAGATCATGCCGACGGAGGTCCGGGTTGCCGGTTTCTCACTGGCCTACA
GCCTGGCGACCGCTGTCTTTGGCGGATTTACACCGGTCATTTCTACCGCGCTGATCGAGTACACCGGCGATAAGGCATCC
CCCGGCTACTGGATGAGTTTTGCTGCGCTATGTGCATTGCTGGCGACATTCTATCTGTACCGTCGCAGCCCATTATCTTT
ACAAACAGCCGCTTAA

Upstream 100 bases:

>100_bases
CATCAGCTGATGCGTTTATTGCGCTTATTTACTTTATTGACCTGATCCAGTCTGCAGAAACAGGCGCAGTAAAAGCGTCA
ATGACAAACTGGAGATTAAG

Downstream 100 bases:

>100_bases
ATCAGGGATTACGTCATGCAAAAAATCTATCGTTCATTGCTCGCCACGTTGGTGATTTTCTCTGTCAGTACGGGAGTACA
AGCTAAAGAAGTGACCGTCA

Product: major facilitator superfamily metabolite/H(+) symporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MHSSNAPMTRRARISAILRVTSGNFLEQFDFFLFGFYATYIAHTFFPASSEFASLMMTFAVFGAGFLMRPIGAIVLGAYI
DKVGRRKGLIVTLSIMAAGTFLIVLIPSYQTIGIWAPLLVLGGRLLQGFSAGAELGGVSVYLAEIATPGRKGFYTSWQSG
SQQVAIMVAAAMGFALNAVMEESAIREWGWRLPFLFGCLIVPFIFFLRRKLEETQEFNARRNHLAMREVFKTLLANWQVV
IAGMLMVAMTTTAFYLITVYAPTFGKKVLMLSASDSLLVTLLVAISNFLWLPVGGALSDRFGRKPVLITMALLALVTAYP
ALGLLAAAPSFSMMLTVLLWLSFIYGLYNGAMIPALTEIMPTEVRVAGFSLAYSLATAVFGGFTPVISTALIEYTGDKAS
PGYWMSFAALCALLATFYLYRRSPLSLQTAA

Sequences:

>Translated_431_residues
MHSSNAPMTRRARISAILRVTSGNFLEQFDFFLFGFYATYIAHTFFPASSEFASLMMTFAVFGAGFLMRPIGAIVLGAYI
DKVGRRKGLIVTLSIMAAGTFLIVLIPSYQTIGIWAPLLVLGGRLLQGFSAGAELGGVSVYLAEIATPGRKGFYTSWQSG
SQQVAIMVAAAMGFALNAVMEESAIREWGWRLPFLFGCLIVPFIFFLRRKLEETQEFNARRNHLAMREVFKTLLANWQVV
IAGMLMVAMTTTAFYLITVYAPTFGKKVLMLSASDSLLVTLLVAISNFLWLPVGGALSDRFGRKPVLITMALLALVTAYP
ALGLLAAAPSFSMMLTVLLWLSFIYGLYNGAMIPALTEIMPTEVRVAGFSLAYSLATAVFGGFTPVISTALIEYTGDKAS
PGYWMSFAALCALLATFYLYRRSPLSLQTAA
>Mature_431_residues
MHSSNAPMTRRARISAILRVTSGNFLEQFDFFLFGFYATYIAHTFFPASSEFASLMMTFAVFGAGFLMRPIGAIVLGAYI
DKVGRRKGLIVTLSIMAAGTFLIVLIPSYQTIGIWAPLLVLGGRLLQGFSAGAELGGVSVYLAEIATPGRKGFYTSWQSG
SQQVAIMVAAAMGFALNAVMEESAIREWGWRLPFLFGCLIVPFIFFLRRKLEETQEFNARRNHLAMREVFKTLLANWQVV
IAGMLMVAMTTTAFYLITVYAPTFGKKVLMLSASDSLLVTLLVAISNFLWLPVGGALSDRFGRKPVLITMALLALVTAYP
ALGLLAAAPSFSMMLTVLLWLSFIYGLYNGAMIPALTEIMPTEVRVAGFSLAYSLATAVFGGFTPVISTALIEYTGDKAS
PGYWMSFAALCALLATFYLYRRSPLSLQTAA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=422, Percent_Identity=34.5971563981043, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1790550, Length=411, Percent_Identity=31.3868613138686, Blast_Score=211, Evalue=8e-56,
Organism=Escherichia coli, GI1788292, Length=403, Percent_Identity=30.7692307692308, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789941, Length=430, Percent_Identity=30.9302325581395, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI87082231, Length=259, Percent_Identity=25.0965250965251, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47010; Mature: 47010

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCCCCC
>Mature Secondary Structure
MHSSNAPMTRRARISAILRVTSGNFLEQFDFFLFGFYATYIAHTFFPASSEFASLMMTFA
CCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
VFGAGFLMRPIGAIVLGAYIDKVGRRKGLIVTLSIMAAGTFLIVLIPSYQTIGIWAPLLV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHCEEEEEEECCCCHHHHHHHHHH
LGGRLLQGFSAGAELGGVSVYLAEIATPGRKGFYTSWQSGSQQVAIMVAAAMGFALNAVM
HHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
EESAIREWGWRLPFLFGCLIVPFIFFLRRKLEETQEFNARRNHLAMREVFKTLLANWQVV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAGMLMVAMTTTAFYLITVYAPTFGKKVLMLSASDSLLVTLLVAISNFLWLPVGGALSDR
HHHHHHHHHHHHHHHHHHEECCCCCCEEEEEECCCHHHHHHHHHHHHHHEECCCCHHHHH
FGRKPVLITMALLALVTAYPALGLLAAAPSFSMMLTVLLWLSFIYGLYNGAMIPALTEIM
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
PTEVRVAGFSLAYSLATAVFGGFTPVISTALIEYTGDKASPGYWMSFAALCALLATFYLY
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
RRSPLSLQTAA
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]