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The map label for this gene is rfaQ [H]

Identifier: 157371388

GI number: 157371388

Start: 3466753

End: 3467853

Strand: Direct

Name: rfaQ [H]

Synonym: Spro_3149

Alternate gene names: 157371388

Gene position: 3466753-3467853 (Clockwise)

Preceding gene: 157371387

Following gene: 157371397

Centisome position: 63.62

GC content: 52.5

Gene sequence:

>1101_bases
ATGTCGAAACAGTGGAAAATAACAGCGGCAAACGCCTGTCTTAAGATGTACACCCGCTTGGGCGGCAACTTCCAGCAGAA
AGCCAGTTTCAAGCCGGAACTGGCCTTTGAGCGTATCGTTATTTTTTCTACCACTGCCCTTGGCGACCTGATGTTTAATA
CGCCGGCCATCCGCGCGGTGCGCGAACGCTACCCAACGGCCAATATCATCCTGATTTCCAGCCATAAAAATAAACAGCTG
GTCGCGACCAGCCGTTACTTTAATGCCGTCATTTATTGGGATCATAAAGCCAAAGACATGCTCAGCGTTATTCACCAGTT
GCGTAAATATAAGCCGCAACTGGCGATCATCCTGCACTCAAAATCCCCTTACGACGTGATTGTCGCCATTACTGCCGGTT
GCCAATATGTGTTCAAGGACGCCTATGGCAACAAGGCCACCGGCATGGAGCCCTGGTTGAGCGGCGTGAGCCAAAGTACC
GGCGGCCATTTAATTCAGCGTAAGCTGGATTTAGTGGCACAGTTGGGTTGCAACACCGATAACAGCGAAATGTTTATTCC
CGTTGAGTTTGCGACGATGAATAAAACCGCGGATAAAATCCCGATCGGTTTTCAAATGGGGGCTTCTGAAACGCTGCGTT
GCTGGCCCGTCGGTCAGTTTATTCGCCTGGCAAAACTGCTGCTGGCGCAGTCGCCGCACCACCAAATCGAATTAATCGGC
TCGCCGAAAGAGCTGTCCATAGAACGAGAATTTATCGCGGGGCTGACAGAGGCCGAACGGCAACGCGTGGTCAGTCATAT
CGGCAAAACCACCCTGCCGCAACTGCTGGCGGTGATGTCAAACCTGCAGGTATTGGTCACTGGCGACACCGGCCCTTTGC
ATCTGGCCATTGCCCTGAAAACCCCGACCATCAGCCTGTTTGTCACCGCCAATCCGCAGCACACCGGCCCTTATCAAAAT
CGCGAACTGCATCAGGTAATGAACGTGTCGGTTGATGCACAAACGCTCACTACGGCTCAACGCCGCCAACCCCTCAGCAT
CATCACTGAAAGTCAGGTGTTTGAAAAAGTGACGAGCGCGTTGGCACTAACGCGCATTTGA

Upstream 100 bases:

>100_bases
GCATATGTAGCAAACTATTGCCGACCATTGGCCCACCAGGACAAACATCATATGATGCAGATGCCAGACCGGCGCGCCAA
TTAACTCGAAGTGAAACAAG

Downstream 100 bases:

>100_bases
GGTGGGTTGGGCGAACACCACAGCCAAGGGGAACCAAAACAGGTACCAAAATTCGGCCGGATTCGCGATAACAAACATGC
CCTGTGAGCAGTAGAAGATC

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 365

Protein sequence:

>366_residues
MSKQWKITAANACLKMYTRLGGNFQQKASFKPELAFERIVIFSTTALGDLMFNTPAIRAVRERYPTANIILISSHKNKQL
VATSRYFNAVIYWDHKAKDMLSVIHQLRKYKPQLAIILHSKSPYDVIVAITAGCQYVFKDAYGNKATGMEPWLSGVSQST
GGHLIQRKLDLVAQLGCNTDNSEMFIPVEFATMNKTADKIPIGFQMGASETLRCWPVGQFIRLAKLLLAQSPHHQIELIG
SPKELSIEREFIAGLTEAERQRVVSHIGKTTLPQLLAVMSNLQVLVTGDTGPLHLAIALKTPTISLFVTANPQHTGPYQN
RELHQVMNVSVDAQTLTTAQRRQPLSIITESQVFEKVTSALALTRI

Sequences:

>Translated_366_residues
MSKQWKITAANACLKMYTRLGGNFQQKASFKPELAFERIVIFSTTALGDLMFNTPAIRAVRERYPTANIILISSHKNKQL
VATSRYFNAVIYWDHKAKDMLSVIHQLRKYKPQLAIILHSKSPYDVIVAITAGCQYVFKDAYGNKATGMEPWLSGVSQST
GGHLIQRKLDLVAQLGCNTDNSEMFIPVEFATMNKTADKIPIGFQMGASETLRCWPVGQFIRLAKLLLAQSPHHQIELIG
SPKELSIEREFIAGLTEAERQRVVSHIGKTTLPQLLAVMSNLQVLVTGDTGPLHLAIALKTPTISLFVTANPQHTGPYQN
RELHQVMNVSVDAQTLTTAQRRQPLSIITESQVFEKVTSALALTRI
>Mature_365_residues
SKQWKITAANACLKMYTRLGGNFQQKASFKPELAFERIVIFSTTALGDLMFNTPAIRAVRERYPTANIILISSHKNKQLV
ATSRYFNAVIYWDHKAKDMLSVIHQLRKYKPQLAIILHSKSPYDVIVAITAGCQYVFKDAYGNKATGMEPWLSGVSQSTG
GHLIQRKLDLVAQLGCNTDNSEMFIPVEFATMNKTADKIPIGFQMGASETLRCWPVGQFIRLAKLLLAQSPHHQIELIGS
PKELSIEREFIAGLTEAERQRVVSHIGKTTLPQLLAVMSNLQVLVTGDTGPLHLAIALKTPTISLFVTANPQHTGPYQNR
ELHQVMNVSVDAQTLTTAQRRQPLSIITESQVFEKVTSALALTRI

Specific function: Catalyzes heptose transfer to the lipopolysaccharide core. It transfers a heptose, called heptose(III), to the heptose(II) of the inner core [H]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790062, Length=283, Percent_Identity=24.7349823321555, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011916 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 40741; Mature: 40610

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQWKITAANACLKMYTRLGGNFQQKASFKPELAFERIVIFSTTALGDLMFNTPAIRAV
CCCCEEEEHHHHHHHHHHHHCCCCHHHCCCCCCHHHEEEEEEEEHHHHHHHHCCHHHHHH
RERYPTANIILISSHKNKQLVATSRYFNAVIYWDHKAKDMLSVIHQLRKYKPQLAIILHS
HHHCCCCEEEEEECCCCCEEEEEEEEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEC
KSPYDVIVAITAGCQYVFKDAYGNKATGMEPWLSGVSQSTGGHLIQRKLDLVAQLGCNTD
CCCCEEEEEEECCCCEEEHHCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
NSEMFIPVEFATMNKTADKIPIGFQMGASETLRCWPVGQFIRLAKLLLAQSPHHQIELIG
CCCEEEEEEEEECCCCCCCCCCEEECCCCCCEEEECHHHHHHHHHHHHHCCCCCEEEEEC
SPKELSIEREFIAGLTEAERQRVVSHIGKTTLPQLLAVMSNLQVLVTGDTGPLHLAIALK
CCHHHCCHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCCEEEEEEEE
TPTISLFVTANPQHTGPYQNRELHQVMNVSVDAQTLTTAQRRQPLSIITESQVFEKVTSA
CCEEEEEEEECCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LALTRI
HHHCCC
>Mature Secondary Structure 
SKQWKITAANACLKMYTRLGGNFQQKASFKPELAFERIVIFSTTALGDLMFNTPAIRAV
CCCEEEEHHHHHHHHHHHHCCCCHHHCCCCCCHHHEEEEEEEEHHHHHHHHCCHHHHHH
RERYPTANIILISSHKNKQLVATSRYFNAVIYWDHKAKDMLSVIHQLRKYKPQLAIILHS
HHHCCCCEEEEEECCCCCEEEEEEEEEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEC
KSPYDVIVAITAGCQYVFKDAYGNKATGMEPWLSGVSQSTGGHLIQRKLDLVAQLGCNTD
CCCCEEEEEEECCCCEEEHHCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCC
NSEMFIPVEFATMNKTADKIPIGFQMGASETLRCWPVGQFIRLAKLLLAQSPHHQIELIG
CCCEEEEEEEEECCCCCCCCCCEEECCCCCCEEEECHHHHHHHHHHHHHCCCCCEEEEEC
SPKELSIEREFIAGLTEAERQRVVSHIGKTTLPQLLAVMSNLQVLVTGDTGPLHLAIALK
CCHHHCCHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEECCCCCEEEEEEEE
TPTISLFVTANPQHTGPYQNRELHQVMNVSVDAQTLTTAQRRQPLSIITESQVFEKVTSA
CCEEEEEEEECCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
LALTRI
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Transferring other glycosyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732225; 1447141; 8041620; 9278503 [H]