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The map label for this gene is pcaJ [H]

Identifier: 157371278

GI number: 157371278

Start: 3347133

End: 3347813

Strand: Direct

Name: pcaJ [H]

Synonym: Spro_3038

Alternate gene names: 157371278

Gene position: 3347133-3347813 (Clockwise)

Preceding gene: 157371277

Following gene: 157371279

Centisome position: 61.43

GC content: 58.3

Gene sequence:

>681_bases
ATGACCAAACTTACACATCAACAGTTGGCCGAACGTATTGCCCGCGATATCCCGGAAGGTGCCTACGTCAACCTGGGCAT
TGGGATCCCAACCCAGATAGCCAACTTCCTGCCGGCGGATAAAGAGATCTTCCTGCACAGCGAAAACGGCATTCTCGGGA
TGGGCCCGGCACCGGCGGCCGGCCAGGAAGATCCCGAACTGATCAACGCCGGTAAACAGCCGGTCACCCTGCTCAAAGGC
GGCTGCTTTTTCCATCACGGCGACTCTTTTGCCATGATGCGCGGTGGTCATCTGGATATCTGCGTGCTCGGCGCCTATCA
GGTTTCCGAACGCGGCGATCTGGCCAACTGGAGTACCGGCGCAGCGGGGGCAATCCCGGCGGTCGGCGGCGCAATGGATC
TGGCGATTGGCGCCCGTCAGGTGTTTGTGATGACCGAACACCTGACCAAAAAAGGCGAATGCAAAATTGTTCAGCACTGT
ACTTATCCCCTGACCGGCGTCGGCTGTATCGATCGCATCTACAGCGATCTGGCGGTGATGGACGTGACTTCTCAGGGTCT
GGTGGTACGCGAAATATTTGGTGGGCTGACCCCGCAGCAATTGCAAGCAGTCACCCCGGTCGAACTGACCTTTGCCCTTC
AGCACGGAGAACCACCGCATGATCCCCGCTTACCTGTGTGA

Upstream 100 bases:

>100_bases
AGTTGGATCCTGAAAATATCATTACGCCGGGGATCTTCGTGCAACGCCTGGTGACTACACCCGATCACCCTGCGCCAGCG
TCGGCTTAAGGAGCGCCCCC

Downstream 100 bases:

>100_bases
TGCCGTCCGTACGCCTTTTGGTCGACTCAACGGCAGCCTGGCCAGCATTCGCGCCGACGACCTGGCCGCCCTGCCGCTCA
AAGCGTTGCAGGCGCGCCAC

Product: 3-oxoacid CoA-transferase subunit B

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MTKLTHQQLAERIARDIPEGAYVNLGIGIPTQIANFLPADKEIFLHSENGILGMGPAPAAGQEDPELINAGKQPVTLLKG
GCFFHHGDSFAMMRGGHLDICVLGAYQVSERGDLANWSTGAAGAIPAVGGAMDLAIGARQVFVMTEHLTKKGECKIVQHC
TYPLTGVGCIDRIYSDLAVMDVTSQGLVVREIFGGLTPQQLQAVTPVELTFALQHGEPPHDPRLPV

Sequences:

>Translated_226_residues
MTKLTHQQLAERIARDIPEGAYVNLGIGIPTQIANFLPADKEIFLHSENGILGMGPAPAAGQEDPELINAGKQPVTLLKG
GCFFHHGDSFAMMRGGHLDICVLGAYQVSERGDLANWSTGAAGAIPAVGGAMDLAIGARQVFVMTEHLTKKGECKIVQHC
TYPLTGVGCIDRIYSDLAVMDVTSQGLVVREIFGGLTPQQLQAVTPVELTFALQHGEPPHDPRLPV
>Mature_225_residues
TKLTHQQLAERIARDIPEGAYVNLGIGIPTQIANFLPADKEIFLHSENGILGMGPAPAAGQEDPELINAGKQPVTLLKGG
CFFHHGDSFAMMRGGHLDICVLGAYQVSERGDLANWSTGAAGAIPAVGGAMDLAIGARQVFVMTEHLTKKGECKIVQHCT
YPLTGVGCIDRIYSDLAVMDVTSQGLVVREIFGGLTPQQLQAVTPVELTFALQHGEPPHDPRLPV

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI11545841, Length=202, Percent_Identity=47.5247524752475, Blast_Score=184, Evalue=6e-47,
Organism=Homo sapiens, GI4557817, Length=202, Percent_Identity=45.5445544554455, Blast_Score=176, Evalue=2e-44,
Organism=Escherichia coli, GI1788552, Length=206, Percent_Identity=42.2330097087379, Blast_Score=166, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17531517, Length=211, Percent_Identity=48.3412322274881, Blast_Score=188, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24655794, Length=212, Percent_Identity=43.8679245283019, Blast_Score=173, Evalue=8e-44,
Organism=Drosophila melanogaster, GI21358325, Length=212, Percent_Identity=43.8679245283019, Blast_Score=173, Evalue=8e-44,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 24072; Mature: 23941

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLTHQQLAERIARDIPEGAYVNLGIGIPTQIANFLPADKEIFLHSENGILGMGPAPAA
CCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCEEEEECCCCEEECCCCCCC
GQEDPELINAGKQPVTLLKGGCFFHHGDSFAMMRGGHLDICVLGAYQVSERGDLANWSTG
CCCCHHHHHCCCCCEEEEECCEEEEECCCEEEEECCCEEEEEEEEEEECCCCCCCCCCCC
AAGAIPAVGGAMDLAIGARQVFVMTEHLTKKGECKIVQHCTYPLTGVGCIDRIYSDLAVM
CCCCCCCCCCHHHHHHCCEEEEEEEHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHEEE
DVTSQGLVVREIFGGLTPQQLQAVTPVELTFALQHGEPPHDPRLPV
EECCCCEEHHHHHCCCCHHHHHCCCCEEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TKLTHQQLAERIARDIPEGAYVNLGIGIPTQIANFLPADKEIFLHSENGILGMGPAPAA
CCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCCCCEEEEECCCCEEECCCCCCC
GQEDPELINAGKQPVTLLKGGCFFHHGDSFAMMRGGHLDICVLGAYQVSERGDLANWSTG
CCCCHHHHHCCCCCEEEEECCEEEEECCCEEEEECCCEEEEEEEEEEECCCCCCCCCCCC
AAGAIPAVGGAMDLAIGARQVFVMTEHLTKKGECKIVQHCTYPLTGVGCIDRIYSDLAVM
CCCCCCCCCCHHHHHHCCEEEEEEEHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHEEE
DVTSQGLVVREIFGGLTPQQLQAVTPVELTFALQHGEPPHDPRLPV
EECCCCEEHHHHHCCCCHHHHHCCCCEEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8063101; 8125318 [H]