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The map label for this gene is norW

Identifier: 157371274

GI number: 157371274

Start: 3340676

End: 3341815

Strand: Direct

Name: norW

Synonym: Spro_3034

Alternate gene names: 157371274

Gene position: 3340676-3341815 (Clockwise)

Preceding gene: 157371273

Following gene: 157371275

Centisome position: 61.31

GC content: 56.58

Gene sequence:

>1140_bases
ATGAACCCCGGTATCCTGATCGTCGGCTCCGGCTTTGCCGCCCGACAGTTGGTCAAGAATCTGCGCAGGCAAGATGGCGA
AGTCCCTATTCGGCTGATAGCCGCCGACAGTTGCGATGAATATAACAAACCGGAACTGAGCCATGTATTCAGCCTAAACC
AAACCGCTGACGCCCTGACCCGGCAAAGCTTCGGTGACTTCGCCGAACAGTTCCAACTGACGGTACACCCCCATACGCGT
ATTACGGCGATAGATACGCAGCAAAAAATGGTGCGCAGCGGAGAGCAAGTCTGGCATTACGACAAACTGGTTTTGGCGAT
CGGCGCTGCGGCCATGGTGCCGCAGGTGCCGGGCCATGAGTTAATGCTGACGCTCAACAGCCAACAGGAATTCCGCGACA
GCCAATTGACCCTGCTCCAGGCGCAACGGGTGTTGATTTTGGGCGGGGGGCTGATCGGCTGTGAACTGGCGATGGACATG
TGCCGGGCCGGTAAACAGGTCACCCTGGTAGACAGATCCGGCAGCCTGCTATCGGGGTTAATGCCGGTTGAAGCGAGCTG
CCGCCTGCAACATGCTCTGCAACAGATGGGGGTAGAATTGCTGCTCAACCAGCAACTGGGCGCACTGACACAACATGATG
AGGGGATACAAGCCACGCTGAGCAACGGTCGCCAATTGTGGGTTGATGCAGCGATAGCGTCGGTCGGTTTGCGGCCAAAC
GTCGGGCTGGCGCGTCAGGCCGGTTTGCAGATTAATCGCGGCATCCAGGTGAATAATCGTTTACAGACCTCGCAAGTGGA
TGTTTACGCTCTTGGTGATTGCGCGGAGATCGAGGGGCAACTGTTACCGTTCCTCCAGCCGATTCAGTTCAGCGCCATGA
CCCTGGCAAAAAACCTGCTGGGTGCGGCGGAAGCGGTGAAGCTGCCCGCCATGCTGGTCAAGGTGAAAACCCCCAGCTTG
CCGCTGCAGCTGGCAGGGGAAACCCGTCGGCAGGATTTGAGCTGGACAATTGTCGCTGAGCCGCAGGGAATGATCGCTAA
AGGCTTTGATCAGCACCAGCAGTTACGCGCGTTTATCGTTAGCGAAGATCATATGAAACTGGCCTTCGGTTTACTGAAAG
AATTAAATGCCCTGACCTAA

Upstream 100 bases:

>100_bases
TGGCACCCGGAACCTGCTGGCAAGAGGTGCCGGATGATTTCCTGTGCCCGGAATGCGCCATGGGTAAAGAGGTGTTCGAC
CCACTCTCTTCGGAGGCAAA

Downstream 100 bases:

>100_bases
TGAGAGTGTCCTGGTGACACAACGCTGATGGTGGCATTTTGTCCGCTATCAACGTTTAGCGGCTCCTTACGCCAACAGGG
GGTAATCTGTTGGCGTCCCC

Product: nitric oxide reductase

Products: NAD; reduced flavorubredoxin; H2O [C]

Alternate protein names: Flavorubredoxin reductase; FlRd-reductase; FlavoRb reductase

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MNPGILIVGSGFAARQLVKNLRRQDGEVPIRLIAADSCDEYNKPELSHVFSLNQTADALTRQSFGDFAEQFQLTVHPHTR
ITAIDTQQKMVRSGEQVWHYDKLVLAIGAAAMVPQVPGHELMLTLNSQQEFRDSQLTLLQAQRVLILGGGLIGCELAMDM
CRAGKQVTLVDRSGSLLSGLMPVEASCRLQHALQQMGVELLLNQQLGALTQHDEGIQATLSNGRQLWVDAAIASVGLRPN
VGLARQAGLQINRGIQVNNRLQTSQVDVYALGDCAEIEGQLLPFLQPIQFSAMTLAKNLLGAAEAVKLPAMLVKVKTPSL
PLQLAGETRRQDLSWTIVAEPQGMIAKGFDQHQQLRAFIVSEDHMKLAFGLLKELNALT

Sequences:

>Translated_379_residues
MNPGILIVGSGFAARQLVKNLRRQDGEVPIRLIAADSCDEYNKPELSHVFSLNQTADALTRQSFGDFAEQFQLTVHPHTR
ITAIDTQQKMVRSGEQVWHYDKLVLAIGAAAMVPQVPGHELMLTLNSQQEFRDSQLTLLQAQRVLILGGGLIGCELAMDM
CRAGKQVTLVDRSGSLLSGLMPVEASCRLQHALQQMGVELLLNQQLGALTQHDEGIQATLSNGRQLWVDAAIASVGLRPN
VGLARQAGLQINRGIQVNNRLQTSQVDVYALGDCAEIEGQLLPFLQPIQFSAMTLAKNLLGAAEAVKLPAMLVKVKTPSL
PLQLAGETRRQDLSWTIVAEPQGMIAKGFDQHQQLRAFIVSEDHMKLAFGLLKELNALT
>Mature_379_residues
MNPGILIVGSGFAARQLVKNLRRQDGEVPIRLIAADSCDEYNKPELSHVFSLNQTADALTRQSFGDFAEQFQLTVHPHTR
ITAIDTQQKMVRSGEQVWHYDKLVLAIGAAAMVPQVPGHELMLTLNSQQEFRDSQLTLLQAQRVLILGGGLIGCELAMDM
CRAGKQVTLVDRSGSLLSGLMPVEASCRLQHALQQMGVELLLNQQLGALTQHDEGIQATLSNGRQLWVDAAIASVGLRPN
VGLARQAGLQINRGIQVNNRLQTSQVDVYALGDCAEIEGQLLPFLQPIQFSAMTLAKNLLGAAEAVKLPAMLVKVKTPSL
PLQLAGETRRQDLSWTIVAEPQGMIAKGFDQHQQLRAFIVSEDHMKLAFGLLKELNALT

Specific function: One of at least two accessory proteins for anaerobic nitric oxide (NO) reductase. Reduces the rubredoxin moiety of NO reductase

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family

Homologues:

Organism=Homo sapiens, GI21389617, Length=276, Percent_Identity=23.5507246376812, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI226437568, Length=276, Percent_Identity=23.5507246376812, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI65787454, Length=276, Percent_Identity=23.5507246376812, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1789065, Length=375, Percent_Identity=65.3333333333333, Blast_Score=493, Evalue=1e-141,
Organism=Escherichia coli, GI1788892, Length=275, Percent_Identity=28.3636363636364, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1789765, Length=322, Percent_Identity=25.4658385093168, Blast_Score=85, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17559934, Length=281, Percent_Identity=25.6227758007117, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24639257, Length=281, Percent_Identity=22.7758007117438, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI281359715, Length=281, Percent_Identity=22.7758007117438, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI281359713, Length=281, Percent_Identity=22.7758007117438, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24639250, Length=281, Percent_Identity=22.7758007117438, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI18543267, Length=281, Percent_Identity=22.7758007117438, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24639252, Length=281, Percent_Identity=22.7758007117438, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NORW_SERP5 (A8GG95)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479263.1
- ProteinModelPortal:   A8GG95
- SMR:   A8GG95
- STRING:   A8GG95
- GeneID:   5604109
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3034
- eggNOG:   COG0446
- HOGENOM:   HBG542563
- OMA:   DCVISAA
- ProtClustDB:   PRK04965
- BioCyc:   SPRO399741:SPRO_3034-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01313
- InterPro:   IPR013027
- InterPro:   IPR001327
- PRINTS:   PR00368

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2

EC number: NA

Molecular weight: Translated: 41402; Mature: 41402

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPGILIVGSGFAARQLVKNLRRQDGEVPIRLIAADSCDEYNKPELSHVFSLNQTADALT
CCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHEECCHHHHHHH
RQSFGDFAEQFQLTVHPHTRITAIDTQQKMVRSGEQVWHYDKLVLAIGAAAMVPQVPGHE
HHHHHHHHHHEEEEECCCCEEEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCE
LMLTLNSQQEFRDSQLTLLQAQRVLILGGGLIGCELAMDMCRAGKQVTLVDRSGSLLSGL
EEEEECCHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHCC
MPVEASCRLQHALQQMGVELLLNQQLGALTQHDEGIQATLSNGRQLWVDAAIASVGLRPN
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHCCCCEEEEEHHHHHCCCCCC
VGLARQAGLQINRGIQVNNRLQTSQVDVYALGDCAEIEGQLLPFLQPIQFSAMTLAKNLL
CCCHHHCCCEEECCEEECCCEECCCEEEEECCCHHHCCCCCCCHHCHHHHHHHHHHHHHH
GAAEAVKLPAMLVKVKTPSLPLQLAGETRRQDLSWTIVAEPQGMIAKGFDQHQQLRAFIV
HHHHHHHCCEEEEEEECCCCCEEECCCCCCCCCEEEEEECCCCCHHCCCCHHHHHHHHEE
SEDHMKLAFGLLKELNALT
CCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNPGILIVGSGFAARQLVKNLRRQDGEVPIRLIAADSCDEYNKPELSHVFSLNQTADALT
CCCCEEEEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHEECCHHHHHHH
RQSFGDFAEQFQLTVHPHTRITAIDTQQKMVRSGEQVWHYDKLVLAIGAAAMVPQVPGHE
HHHHHHHHHHEEEEECCCCEEEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCE
LMLTLNSQQEFRDSQLTLLQAQRVLILGGGLIGCELAMDMCRAGKQVTLVDRSGSLLSGL
EEEEECCHHHHHHHHHHHHHHCEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHCC
MPVEASCRLQHALQQMGVELLLNQQLGALTQHDEGIQATLSNGRQLWVDAAIASVGLRPN
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCHHHHHCCCCEEEEEHHHHHCCCCCC
VGLARQAGLQINRGIQVNNRLQTSQVDVYALGDCAEIEGQLLPFLQPIQFSAMTLAKNLL
CCCHHHCCCEEECCEEECCCEECCCEEEEECCCHHHCCCCCCCHHCHHHHHHHHHHHHHH
GAAEAVKLPAMLVKVKTPSLPLQLAGETRRQDLSWTIVAEPQGMIAKGFDQHQQLRAFIV
HHHHHHHCCEEEEEEECCCCCEEECCCCCCCCCEEEEEECCCCCHHCCCCHHHHHHHHEE
SEDHMKLAFGLLKELNALT
CCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; oxidized flavorubredoxin; O2 [C]

Specific reaction: NADH + oxidized flavorubredoxin + O2 = NAD + reduced flavorubredoxin + H2O [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA