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The map label for this gene is nasA [H]

Identifier: 157371145

GI number: 157371145

Start: 3192369

End: 3195020

Strand: Reverse

Name: nasA [H]

Synonym: Spro_2905

Alternate gene names: 157371145

Gene position: 3195020-3192369 (Counterclockwise)

Preceding gene: 157371146

Following gene: 157371144

Centisome position: 58.64

GC content: 61.24

Gene sequence:

>2652_bases
ATGAGCGCCGTACATTGCACCACCTGCCCCTACTGTGGCGTGGGCTGCGGGGTCGCCATTGAACAGCGCAGCAACGGTGA
ACTGACGGTCGGCGGCGACGTTACGCATCCGGCCAATCAGGGACGGCTGTGTGTCAAAGGCAGCGCGCTGGGCGAGACGC
TGAGCCTGACGGGCCGCCTGTTACAGCCGCAGGTCGATGGCCTGCCGGTTAGCTGGGATCAGGCGCTTGAGCAGGTGGCA
CAGCGTTTTAGCCAGATTATTGAGCAATATGGCCCGCAGGCGGTGGCGTTTTATGCTTCCGGTCAGCTGCTGACCGAAGA
TTATTACGTGGCAAACAAACTGATGAAGGGATTTATTGGTGCGGCCAATATCGACACCAATTCCCGGCTGTGCATGGCGT
CGGCGGTAGTCGGCTACAAGCGGGCATTCGGTGGGGATGTGGTGCCTTGTTGTTATGAAGATATCGAACAGGCGGATCTG
GTGATATTGACCGGTTCCAACACCGCCTGGGCGCATCCGGTGGTCTACCAACGGTTGGTGAAGGCCCGTAAGTTGCGCCC
GCAAATGCGGGTGGTGGTGATTGACCCGCGTGAGACGGCGACCTGCGATATTGCCGACTTGCATTTGCCGCTGCGGCCCG
GCAGCGATGCGGCTTTGTTTACCGGAGGGCTGCACTGGCTGGCTGAACAGCAGGCTCTGGATACCGCCTTTTTGCAACGG
CATGCCAGCGGCACAGAGGCTACGCTGGCAGCGGCGGCGGATTGGACACCACAGCGGGTGGCCGATTTTTGCGAACTGCC
ACTGAGTGCCGTGCTGGCGTTTTTCCACCTGCTGACCGCGTCCCGCAACTGGGTCACGCTCTATTCGATGGGCATTAACC
AGTCTGCCAGTGGCTCGGATAAATGCAATGCGATTATCAATCTGCACCTGGCGGGAGGGCGCATTGGCCGCGCTGGCAGC
GGCCCGTTTTCGATCACCGGTCAACCCAATGCCATGGGCGGGCGGGAAGTTGGTGGATTAGCCAATCAGCTGGCGGCGCA
TATGACGTTCAACCCCGAAGACACCGACCGGGTACAGCGTTTTTGGCAGTCGCCACGCATCGCCCAACAACCGGGGCTGA
ATGCGGTGGATTTATTTCAGGCGATCGCCGCCGGGCAGGTAAAGGCGGTGTGGATTATGGGCACCAACCCGGTGGTTTCC
CTGCCGGATGCCGATGCAGTCAGGCAGGCGCTAAAAAATTGCCCGCTGGTGGTGGTGTCCGAAGTGATGGCGATGACCGA
TACCGCCGAACTGGCACATATCCGCCTGCCGGCATTGGCCTGGGGAGAAAAGGAGGGCAGCGTGACCAATTCCGAACGCT
GTATTTCACGCCAGCGTGCATTTTTGCCGGCGCCGGGGCAAGCCCGTGCCGACTGGTGGATCCTTAGCCAGGTTGCGCAG
CGTATGGGGTATGGTACGGCCTTTGGCTATCAACATCCTTCGGAGATATTCAGTGAGCACGCGGCGCTGTCAGGATTTGA
AAATAACGGCACGCGAGCGTTCGATATCAGTCAACTGGCGGGATGGGATCTACAGCAATGGTTGCAGATGGCACCGGTAC
AGTGGCCGGTCAATGCACGGCATCCTGTGGGTTGTCGACGAATGTTTGACGACGGGCGTTTTTTCCATGCCGATGGCAAG
GCGCGACTGCTGCCGGTGACGCCACGCTTGCCGCAGAATGCGCTATCACCCGGTTATCCACTGGTGCTGAACACCGGTCG
CATCCGCGATCAGTGGCACACCATGACGCGCACCGGCAAGGCCGCGCGGCTGATGCGCCATTTGAGCGAACCCTTCTGCG
AAATACACCCGCAGGATGCGGTCGAACTGGGAATTGCCGATAGCAGTCTGGTTCGCCTGAGCTCGCCCCACGGCTGGATG
CTGGCGCGTGCACAGTACCATCCGGGGCAGCGGCGCGGTAGCCTGTTTGCACCGATGCACTGGAACGGTCAATTCACCGC
GCAGGGTCGGGTCGACAGCCTGATCCCGCCGATAGTGGATGCCGACTCCGGCCAGCCGGAAAGCAAGCATGCGCCAGTGC
GGGTCAGTCATTGGCCCACGAGCTGGCAGGCGGAAATTTTTATTCGTGCCGAGGTTGCCGCCCCCAAGGGTATCTACTGG
AGCCGGGTCGCGCAGGACGGTTTGACTCACTACATTATGGCCGGCCAGCGGCCGGTTGAAGACTGGCAAGCCTGGTTACG
GCGCCATTTCTCGCTGGAGGGCATGACGCTGCAAACGGCACAGTTGGCCCAGCGCGGTCTGCACCTGATTGGCTGGCGAC
AGGGCGAGGTGCAATTGGCGTTTTATGTTCGCCAGCAGGCCCCGCAGCTGGATCGCCCGGCGATCCTCCGCGCCTTTGAA
CAGGCTCCCACCGCAGGGGCGGAACGGCTGGCACTGCTGGCGGGGCGTGGTGCGCCAGGCCAGTCAGCGGCTGGTGCCAC
GATTTGTAGCTGTTTCGGCGTGGGCGAGAATCGCGTCATTGCGGCCATCCAGGCAGGTTGCCACAGCGCAGAGGCATTGG
GTGCCCGTCTGCAATGCGGCACCAACTGTGGCTCCTGCCTGCCGGAGCTAAAAAAACTGATTCAACAGCATGCATCCCAA
CGGGTGGCCTAG

Upstream 100 bases:

>100_bases
TCACCGCCTGGCCGGTGCGGGTAGAAGCAGGCCGTGTGTGGGTATGCAGTACGCCGCTGTCGATACAGGCCAAACTGCCA
GAAACGGCGGAAGTGACGTC

Downstream 100 bases:

>100_bases
CGGAGGTGGTGAGATGAACAATATAGATAAACTGATGCTGGCCGGAGATCTGCGCCAGCCGGTACGTAACGGTGAAGTGT
GGCTGGTGGGCGCCGGGCCG

Product: molybdopterin oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 883; Mature: 882

Protein sequence:

>883_residues
MSAVHCTTCPYCGVGCGVAIEQRSNGELTVGGDVTHPANQGRLCVKGSALGETLSLTGRLLQPQVDGLPVSWDQALEQVA
QRFSQIIEQYGPQAVAFYASGQLLTEDYYVANKLMKGFIGAANIDTNSRLCMASAVVGYKRAFGGDVVPCCYEDIEQADL
VILTGSNTAWAHPVVYQRLVKARKLRPQMRVVVIDPRETATCDIADLHLPLRPGSDAALFTGGLHWLAEQQALDTAFLQR
HASGTEATLAAAADWTPQRVADFCELPLSAVLAFFHLLTASRNWVTLYSMGINQSASGSDKCNAIINLHLAGGRIGRAGS
GPFSITGQPNAMGGREVGGLANQLAAHMTFNPEDTDRVQRFWQSPRIAQQPGLNAVDLFQAIAAGQVKAVWIMGTNPVVS
LPDADAVRQALKNCPLVVVSEVMAMTDTAELAHIRLPALAWGEKEGSVTNSERCISRQRAFLPAPGQARADWWILSQVAQ
RMGYGTAFGYQHPSEIFSEHAALSGFENNGTRAFDISQLAGWDLQQWLQMAPVQWPVNARHPVGCRRMFDDGRFFHADGK
ARLLPVTPRLPQNALSPGYPLVLNTGRIRDQWHTMTRTGKAARLMRHLSEPFCEIHPQDAVELGIADSSLVRLSSPHGWM
LARAQYHPGQRRGSLFAPMHWNGQFTAQGRVDSLIPPIVDADSGQPESKHAPVRVSHWPTSWQAEIFIRAEVAAPKGIYW
SRVAQDGLTHYIMAGQRPVEDWQAWLRRHFSLEGMTLQTAQLAQRGLHLIGWRQGEVQLAFYVRQQAPQLDRPAILRAFE
QAPTAGAERLALLAGRGAPGQSAAGATICSCFGVGENRVIAAIQAGCHSAEALGARLQCGTNCGSCLPELKKLIQQHASQ
RVA

Sequences:

>Translated_883_residues
MSAVHCTTCPYCGVGCGVAIEQRSNGELTVGGDVTHPANQGRLCVKGSALGETLSLTGRLLQPQVDGLPVSWDQALEQVA
QRFSQIIEQYGPQAVAFYASGQLLTEDYYVANKLMKGFIGAANIDTNSRLCMASAVVGYKRAFGGDVVPCCYEDIEQADL
VILTGSNTAWAHPVVYQRLVKARKLRPQMRVVVIDPRETATCDIADLHLPLRPGSDAALFTGGLHWLAEQQALDTAFLQR
HASGTEATLAAAADWTPQRVADFCELPLSAVLAFFHLLTASRNWVTLYSMGINQSASGSDKCNAIINLHLAGGRIGRAGS
GPFSITGQPNAMGGREVGGLANQLAAHMTFNPEDTDRVQRFWQSPRIAQQPGLNAVDLFQAIAAGQVKAVWIMGTNPVVS
LPDADAVRQALKNCPLVVVSEVMAMTDTAELAHIRLPALAWGEKEGSVTNSERCISRQRAFLPAPGQARADWWILSQVAQ
RMGYGTAFGYQHPSEIFSEHAALSGFENNGTRAFDISQLAGWDLQQWLQMAPVQWPVNARHPVGCRRMFDDGRFFHADGK
ARLLPVTPRLPQNALSPGYPLVLNTGRIRDQWHTMTRTGKAARLMRHLSEPFCEIHPQDAVELGIADSSLVRLSSPHGWM
LARAQYHPGQRRGSLFAPMHWNGQFTAQGRVDSLIPPIVDADSGQPESKHAPVRVSHWPTSWQAEIFIRAEVAAPKGIYW
SRVAQDGLTHYIMAGQRPVEDWQAWLRRHFSLEGMTLQTAQLAQRGLHLIGWRQGEVQLAFYVRQQAPQLDRPAILRAFE
QAPTAGAERLALLAGRGAPGQSAAGATICSCFGVGENRVIAAIQAGCHSAEALGARLQCGTNCGSCLPELKKLIQQHASQ
RVA
>Mature_882_residues
SAVHCTTCPYCGVGCGVAIEQRSNGELTVGGDVTHPANQGRLCVKGSALGETLSLTGRLLQPQVDGLPVSWDQALEQVAQ
RFSQIIEQYGPQAVAFYASGQLLTEDYYVANKLMKGFIGAANIDTNSRLCMASAVVGYKRAFGGDVVPCCYEDIEQADLV
ILTGSNTAWAHPVVYQRLVKARKLRPQMRVVVIDPRETATCDIADLHLPLRPGSDAALFTGGLHWLAEQQALDTAFLQRH
ASGTEATLAAAADWTPQRVADFCELPLSAVLAFFHLLTASRNWVTLYSMGINQSASGSDKCNAIINLHLAGGRIGRAGSG
PFSITGQPNAMGGREVGGLANQLAAHMTFNPEDTDRVQRFWQSPRIAQQPGLNAVDLFQAIAAGQVKAVWIMGTNPVVSL
PDADAVRQALKNCPLVVVSEVMAMTDTAELAHIRLPALAWGEKEGSVTNSERCISRQRAFLPAPGQARADWWILSQVAQR
MGYGTAFGYQHPSEIFSEHAALSGFENNGTRAFDISQLAGWDLQQWLQMAPVQWPVNARHPVGCRRMFDDGRFFHADGKA
RLLPVTPRLPQNALSPGYPLVLNTGRIRDQWHTMTRTGKAARLMRHLSEPFCEIHPQDAVELGIADSSLVRLSSPHGWML
ARAQYHPGQRRGSLFAPMHWNGQFTAQGRVDSLIPPIVDADSGQPESKHAPVRVSHWPTSWQAEIFIRAEVAAPKGIYWS
RVAQDGLTHYIMAGQRPVEDWQAWLRRHFSLEGMTLQTAQLAQRGLHLIGWRQGEVQLAFYVRQQAPQLDRPAILRAFEQ
APTAGAERLALLAGRGAPGQSAAGATICSCFGVGENRVIAAIQAGCHSAEALGARLQCGTNCGSCLPELKKLIQQHASQR
VA

Specific function: Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily [H]

Homologues:

Organism=Escherichia coli, GI3868721, Length=720, Percent_Identity=28.1944444444444, Blast_Score=296, Evalue=3e-81,
Organism=Escherichia coli, GI1788534, Length=793, Percent_Identity=27.7427490542245, Blast_Score=275, Evalue=7e-75,
Organism=Escherichia coli, GI3868719, Length=668, Percent_Identity=22.4550898203593, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI3868720, Length=668, Percent_Identity=23.2035928143713, Blast_Score=143, Evalue=4e-35,
Organism=Escherichia coli, GI1787778, Length=474, Percent_Identity=25.5274261603376, Blast_Score=108, Evalue=2e-24,
Organism=Escherichia coli, GI1787870, Length=525, Percent_Identity=25.7142857142857, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI87081797, Length=551, Percent_Identity=22.8675136116152, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI171474008, Length=537, Percent_Identity=22.5325884543762, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI87081994, Length=656, Percent_Identity=22.8658536585366, Blast_Score=70, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR007419
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 96074; Mature: 95943

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVHCTTCPYCGVGCGVAIEQRSNGELTVGGDVTHPANQGRLCVKGSALGETLSLTGRL
CCCEECCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHH
LQPQVDGLPVSWDQALEQVAQRFSQIIEQYGPQAVAFYASGQLLTEDYYVANKLMKGFIG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEHHHHHHHHHHHHHCC
AANIDTNSRLCMASAVVGYKRAFGGDVVPCCYEDIEQADLVILTGSNTAWAHPVVYQRLV
CCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHH
KARKLRPQMRVVVIDPRETATCDIADLHLPLRPGSDAALFTGGLHWLAEQQALDTAFLQR
HHHHCCCCEEEEEECCCCCCEEEEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHH
HASGTEATLAAAADWTPQRVADFCELPLSAVLAFFHLLTASRNWVTLYSMGINQSASGSD
CCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
KCNAIINLHLAGGRIGRAGSGPFSITGQPNAMGGREVGGLANQLAAHMTFNPEDTDRVQR
CCEEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHEECCCCCHHHHHH
FWQSPRIAQQPGLNAVDLFQAIAAGQVKAVWIMGTNPVVSLPDADAVRQALKNCPLVVVS
HHCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCEEECCCHHHHHHHHHCCCHHHHH
EVMAMTDTAELAHIRLPALAWGEKEGSVTNSERCISRQRAFLPAPGQARADWWILSQVAQ
HHHHHCCCHHHHEEECCCEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
RMGYGTAFGYQHPSEIFSEHAALSGFENNGTRAFDISQLAGWDLQQWLQMAPVQWPVNAR
HCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHCCCCHHHHHHHCCCCCCCCCC
HPVGCRRMFDDGRFFHADGKARLLPVTPRLPQNALSPGYPLVLNTGRIRDQWHTMTRTGK
CCCHHHHHHCCCCEEECCCCEEEEECCCCCCHHHCCCCCCEEEECCCCHHHHHHHHHCCH
AARLMRHLSEPFCEIHPQDAVELGIADSSLVRLSSPHGWMLARAQYHPGQRRGSLFAPMH
HHHHHHHHCCHHHHCCCCCCEEECCCCCCEEEECCCCCEEEEEECCCCCCCCCCEEEEEE
WNGQFTAQGRVDSLIPPIVDADSGQPESKHAPVRVSHWPTSWQAEIFIRAEVAAPKGIYW
CCCEEEECCCCHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCCCCHH
SRVAQDGLTHYIMAGQRPVEDWQAWLRRHFSLEGMTLQTAQLAQRGLHLIGWRQGEVQLA
HHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHCCCEEEEECCCCEEEE
FYVRQQAPQLDRPAILRAFEQAPTAGAERLALLAGRGAPGQSAAGATICSCFGVGENRVI
EEEEHHCCCCCCHHHHHHHHHCCCCCHHHEEEEECCCCCCCCCCCHHHHHHCCCCCCCEE
AAIQAGCHSAEALGARLQCGTNCGSCLPELKKLIQQHASQRVA
EEEHHCCCCHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAVHCTTCPYCGVGCGVAIEQRSNGELTVGGDVTHPANQGRLCVKGSALGETLSLTGRL
CCEECCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCEEEECCCCCCHHHHHHHH
LQPQVDGLPVSWDQALEQVAQRFSQIIEQYGPQAVAFYASGQLLTEDYYVANKLMKGFIG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEHHHHHHHHHHHHHCC
AANIDTNSRLCMASAVVGYKRAFGGDVVPCCYEDIEQADLVILTGSNTAWAHPVVYQRLV
CCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHH
KARKLRPQMRVVVIDPRETATCDIADLHLPLRPGSDAALFTGGLHWLAEQQALDTAFLQR
HHHHCCCCEEEEEECCCCCCEEEEEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHHH
HASGTEATLAAAADWTPQRVADFCELPLSAVLAFFHLLTASRNWVTLYSMGINQSASGSD
CCCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
KCNAIINLHLAGGRIGRAGSGPFSITGQPNAMGGREVGGLANQLAAHMTFNPEDTDRVQR
CCEEEEEEEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHEECCCCCHHHHHH
FWQSPRIAQQPGLNAVDLFQAIAAGQVKAVWIMGTNPVVSLPDADAVRQALKNCPLVVVS
HHCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCEEECCCHHHHHHHHHCCCHHHHH
EVMAMTDTAELAHIRLPALAWGEKEGSVTNSERCISRQRAFLPAPGQARADWWILSQVAQ
HHHHHCCCHHHHEEECCCEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
RMGYGTAFGYQHPSEIFSEHAALSGFENNGTRAFDISQLAGWDLQQWLQMAPVQWPVNAR
HCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHCCCCHHHHHHHCCCCCCCCCC
HPVGCRRMFDDGRFFHADGKARLLPVTPRLPQNALSPGYPLVLNTGRIRDQWHTMTRTGK
CCCHHHHHHCCCCEEECCCCEEEEECCCCCCHHHCCCCCCEEEECCCCHHHHHHHHHCCH
AARLMRHLSEPFCEIHPQDAVELGIADSSLVRLSSPHGWMLARAQYHPGQRRGSLFAPMH
HHHHHHHHCCHHHHCCCCCCEEECCCCCCEEEECCCCCEEEEEECCCCCCCCCCEEEEEE
WNGQFTAQGRVDSLIPPIVDADSGQPESKHAPVRVSHWPTSWQAEIFIRAEVAAPKGIYW
CCCEEEECCCCHHCCCCCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCCCCHH
SRVAQDGLTHYIMAGQRPVEDWQAWLRRHFSLEGMTLQTAQLAQRGLHLIGWRQGEVQLA
HHHHHHCCCEEECCCCCCHHHHHHHHHHHCCCCCCEEHHHHHHHCCCEEEEECCCCEEEE
FYVRQQAPQLDRPAILRAFEQAPTAGAERLALLAGRGAPGQSAAGATICSCFGVGENRVI
EEEEHHCCCCCCHHHHHHHHHCCCCCHHHEEEEECCCCCCCCCCCHHHHHHCCCCCCCEE
AAIQAGCHSAEALGARLQCGTNCGSCLPELKKLIQQHASQRVA
EEEHHCCCCHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8468296 [H]