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The map label for this gene is narX [H]

Identifier: 157371139

GI number: 157371139

Start: 3185676

End: 3187460

Strand: Direct

Name: narX [H]

Synonym: Spro_2899

Alternate gene names: 157371139

Gene position: 3185676-3187460 (Clockwise)

Preceding gene: 157371119

Following gene: 157371140

Centisome position: 58.47

GC content: 57.31

Gene sequence:

>1785_bases
ATGAAACGCCTGTTAGCACCACTTTCCATCGTGAATCAGGTGGCCGTCCTGATGTTGCTGCTGGGGATACTGGGCGTGGC
CGGTATGAGCATTTCAGCCTGGATGTCGCAAAGCATTCAGGGCAACGCGCATGCCATCAACAAAGCCGGTTCGCTGCGAA
TGCAAAGTTATCGTCTGCTGGCTCAGGTGCCGCTGAATTCACACAGCGAAGCACTGATCCGTGAGCTGGATCAGGACGCC
AACAGTCCCGATCTTCAGCGTTCAGTGACACAAGAGCAACTGACCGAACAGTTTCAGGCGCTGAATAATTACTGGCAAGA
AACCCTGCTGCCAAGGTTGCGGGCAGCCCGGCAACCGGCGGAGGCAACCCCGCAGGTGGTACATTTCGTTTCCCTGCTCG
ATACGCTGGTCTCGGATATTGACCACCATACCGAGCGGCGCCTGCTGACGGTGACGCTGGTACAGGCGGTGTTTATCGCG
CTGACGCTGCTGCTGCTGGTGGGAACTATCTGTTACCTGCGCCGTCGTCTGCTGCATCCGTGGCGTCAATTGGTGGCGAT
GGCGCTGTCAGCGGGTCGCGGCGATTTCAGCCGCCGTTTTGCCCAGCGTGGCCATCAGGATGAAATGGCCTCACTCGGCG
GCGCGTTGAACACCATGTCCGACGAATTGGCTACCATGTACAACGGGCTGGAGCAGCGGGTGGCAGAAAAAACCGCCGAT
CTGCAGCAAAAGAATCAGGTGCTGGGCTTTTTGTATCACGCCAGCCGTCAGTTGCATGCCAACGAACCATTGTGCAGCCG
CCTGACACCGATCCTCAATCAGTTGCAAACCCTGACGCCACTGCGCGCCATCCAGGTACGCCTGTACGAAAACAACAGTC
AGGAACAGTTTGTACAGCTGTGCGGCAACCAGCCGTTGCGGCCGGAATATTGCAATAATCCGGTGTGCAGCGCCTGCCTG
AGCGACGACAATCAGCAACATGCCGATCATCCTTCGCTGAGCTGGAGCCTGAGTGACAAACTTGGAGATTACGGGGTGAT
CGTTGCGCAACATGCGCCACAGCGCCCGCTGAATGACGAAGATCGGCAGTTGATCAGTACCCTGGTCGAACAGTTGACCA
GCGTGCTGGCGATTGAACGCCAGGTGGATCATCAACAGCAGTTGATGCTGATGGAAGAACGCGCGGCGATAGCCCGTGAG
TTGCATGACTCTATTGCCCAATCATTATCGTGCCTGAAGATGCAAACCAGTTGCCTGCAAATGCAGGGGGCCGAACTTTC
ACCCGCCAACCTGGCGCTGGTACAACAGATGCGCGAAGAGTTAAACATCGCCTACCGCCAGTTGCGTGAACTCTTGACCA
CCTTCCGCCTGCGGCTGACAGAGCCAGGACTGCTGGCAGCCTTGCAGTCGACGGTGCAGGAGTTTAGTCAACGGCTCGGC
ATTTCCATCCAGCTCGACTATCAGTTAACGCCGCGTACGGTGCCCGCTTATCAGGCTATCCACCTGCTGCAAATTGCCCG
CGAAGCGTTAAGCAACATCAACAAACATGCTCAGGCCAGCCAGGTGAGCATTCAGGTAGTCAATCAACGAGGCGAGGTCA
CACTGAGCGTCTGCGATAACGGCTGCGGATTGCCGCCAGACACCGATCGCCCCGATCACTACGGCCTGATAATTATGCGC
GATCGCGCCAAAAGCCTGCACGGCCGCTGCGAGATTTTACCCCGCAGCGGCGGCGGCACTGAGGTCCGGGTGGTATTTAA
ACCGGACAACCACGCCATCGACTAA

Upstream 100 bases:

>100_bases
GAAAAATAACGCGGAATCGTTACCACACCCGCTGATGCCACCTGCGCTTGTCCTCTGGTTACCAGCCGTATCTTGCTCTG
CCTTCGGGGGCCAAAAACCT

Downstream 100 bases:

>100_bases
TGGGAGAAACCATGACCACCGAAACTGCCGCCACTATTTTATTGATTGATGACCACCCGATGCTGCGCAATGGCGTAAAA
CAGCTCATCGGTATGGACTC

Product: nitrate/nitrite sensor protein NarX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MKRLLAPLSIVNQVAVLMLLLGILGVAGMSISAWMSQSIQGNAHAINKAGSLRMQSYRLLAQVPLNSHSEALIRELDQDA
NSPDLQRSVTQEQLTEQFQALNNYWQETLLPRLRAARQPAEATPQVVHFVSLLDTLVSDIDHHTERRLLTVTLVQAVFIA
LTLLLLVGTICYLRRRLLHPWRQLVAMALSAGRGDFSRRFAQRGHQDEMASLGGALNTMSDELATMYNGLEQRVAEKTAD
LQQKNQVLGFLYHASRQLHANEPLCSRLTPILNQLQTLTPLRAIQVRLYENNSQEQFVQLCGNQPLRPEYCNNPVCSACL
SDDNQQHADHPSLSWSLSDKLGDYGVIVAQHAPQRPLNDEDRQLISTLVEQLTSVLAIERQVDHQQQLMLMEERAAIARE
LHDSIAQSLSCLKMQTSCLQMQGAELSPANLALVQQMREELNIAYRQLRELLTTFRLRLTEPGLLAALQSTVQEFSQRLG
ISIQLDYQLTPRTVPAYQAIHLLQIAREALSNINKHAQASQVSIQVVNQRGEVTLSVCDNGCGLPPDTDRPDHYGLIIMR
DRAKSLHGRCEILPRSGGGTEVRVVFKPDNHAID

Sequences:

>Translated_594_residues
MKRLLAPLSIVNQVAVLMLLLGILGVAGMSISAWMSQSIQGNAHAINKAGSLRMQSYRLLAQVPLNSHSEALIRELDQDA
NSPDLQRSVTQEQLTEQFQALNNYWQETLLPRLRAARQPAEATPQVVHFVSLLDTLVSDIDHHTERRLLTVTLVQAVFIA
LTLLLLVGTICYLRRRLLHPWRQLVAMALSAGRGDFSRRFAQRGHQDEMASLGGALNTMSDELATMYNGLEQRVAEKTAD
LQQKNQVLGFLYHASRQLHANEPLCSRLTPILNQLQTLTPLRAIQVRLYENNSQEQFVQLCGNQPLRPEYCNNPVCSACL
SDDNQQHADHPSLSWSLSDKLGDYGVIVAQHAPQRPLNDEDRQLISTLVEQLTSVLAIERQVDHQQQLMLMEERAAIARE
LHDSIAQSLSCLKMQTSCLQMQGAELSPANLALVQQMREELNIAYRQLRELLTTFRLRLTEPGLLAALQSTVQEFSQRLG
ISIQLDYQLTPRTVPAYQAIHLLQIAREALSNINKHAQASQVSIQVVNQRGEVTLSVCDNGCGLPPDTDRPDHYGLIIMR
DRAKSLHGRCEILPRSGGGTEVRVVFKPDNHAID
>Mature_594_residues
MKRLLAPLSIVNQVAVLMLLLGILGVAGMSISAWMSQSIQGNAHAINKAGSLRMQSYRLLAQVPLNSHSEALIRELDQDA
NSPDLQRSVTQEQLTEQFQALNNYWQETLLPRLRAARQPAEATPQVVHFVSLLDTLVSDIDHHTERRLLTVTLVQAVFIA
LTLLLLVGTICYLRRRLLHPWRQLVAMALSAGRGDFSRRFAQRGHQDEMASLGGALNTMSDELATMYNGLEQRVAEKTAD
LQQKNQVLGFLYHASRQLHANEPLCSRLTPILNQLQTLTPLRAIQVRLYENNSQEQFVQLCGNQPLRPEYCNNPVCSACL
SDDNQQHADHPSLSWSLSDKLGDYGVIVAQHAPQRPLNDEDRQLISTLVEQLTSVLAIERQVDHQQQLMLMEERAAIARE
LHDSIAQSLSCLKMQTSCLQMQGAELSPANLALVQQMREELNIAYRQLRELLTTFRLRLTEPGLLAALQSTVQEFSQRLG
ISIQLDYQLTPRTVPAYQAIHLLQIAREALSNINKHAQASQVSIQVVNQRGEVTLSVCDNGCGLPPDTDRPDHYGLIIMR
DRAKSLHGRCEILPRSGGGTEVRVVFKPDNHAID

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the narL protein and of both nitrate/nitrite to the narP protein. NarX probably activates narL and narP by phosphorylation in the presence of nitrate. NarX also plays a

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=594, Percent_Identity=51.1784511784512, Blast_Score=580, Evalue=1e-166,
Organism=Escherichia coli, GI1788812, Length=583, Percent_Identity=31.3893653516295, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI87082311, Length=192, Percent_Identity=31.7708333333333, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR003661
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 66510; Mature: 66510

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCEEEEEECCCHHCCCCCEEEEECCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure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CCCCCCCCCCCCCEEEEEECCCHHCCCCCEEEEECCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]