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The map label for this gene is amsD [H]

Identifier: 157371129

GI number: 157371129

Start: 3172256

End: 3173317

Strand: Reverse

Name: amsD [H]

Synonym: Spro_2889

Alternate gene names: 157371129

Gene position: 3173317-3172256 (Counterclockwise)

Preceding gene: 157371130

Following gene: 157371128

Centisome position: 58.24

GC content: 59.7

Gene sequence:

>1062_bases
ATGCAGCAACGAAAAGTAGCAATCGTGATTGAGAACATCGCCGAAAAAGGCGGTACCGAACGGGTCGCCAGCAGCCTGGC
CAATGCGTTGGCTACGCGTTTGGGGCATCAGGTGGAACTGGTGTCGATCAGCGGTGACCGGGCATTTTACCCGCTGGACG
CCGCGGTGACCCTGCGCTTTATGCCCGGCAGAACGCTGCTGTGGCCCTGGCGGCTGGCGTGGTTTCTGCGGCGCGGCCGT
TATGACGTGATTATCACCGTTTCGATGGGAAAACTCTCGTCGCTGATGGTGCCCTACCTGCGTTTACTGTGTCCGCACAG
CCGTCTGTTGCTGAGTGAGCACGTCAGTTTCCATCAATACGCCTGGCCAATGAAGTGGTTGAAGGTGCTGGTTTATCGGT
TGGGCGACCGCACGGTACTGCTGACCCAAAAAGATCTGGCCACCATCAGCCGCTGGGTGCCGGAACGCAAATGTCAGGTG
ATCGAAAACGTTTCACCTTTCCCGGTGCAATCGCCGCAGACGAAGTTGCAACAGCCGGTGGCGCTGGCGGTCGGTCGGCT
ATGCCATCAGAAAGGGTTTGATCGCCTGATAGCCGCCTGGCAACGGGTGGCACGGCAAACGGCGGGCTGGCAACTGCATA
TCGTCGGTGATGGCCCGGATCGGGCGGCGTTACAGCAACAGATTGACGCCGCCGGGTTGGCTGAACAGGTGAAGTTGCTG
CCGGCGACCGCGGACATTGCCGGCCACTATCGCCAGGCGGCAATGCTGTTAATGACCTCGCGTTACGAAGGGCTGCCGAT
GGTGTTGATTGAAGCCATGAGCTTCGGCCTGCCGCTGGTGGCGTTTGACTGCCAGACCGGGCCAGCCGAACTGATCGATG
ACGAGGGCAATGGCTATCTGGTGGCGGAGGGCGATATCGAGGCCTTCAGCCGACGCACGTTGGCGCTGATCGACGACGGC
CAATTGCGTCAACGTTTTTCTCTGCGCTCGCTGGAGCGGGCGCAACAGTTCATCCCTGAGCGGATTTATCCCCAATGGCA
ACAGCTTATCGCTGGAGGTTAA

Upstream 100 bases:

>100_bases
AGGGCGATCAAACTGTACGGCGACTGCCTGGCCAGGCTAGGGCTGTTTCCGTTGGGGGGGCGTCTGTTACGCAGCGCCCG
TGAGGGATTTCGGAGATGAC

Downstream 100 bases:

>100_bases
GATGGAAAAAGTTTCAGTTATCATGCCGGCTTACAACGCCGCAGGCTTTATCAAACAGTCGATCCTCGGGGTGCTGAACC
AGACCTATCAGGATTATCAC

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MQQRKVAIVIENIAEKGGTERVASSLANALATRLGHQVELVSISGDRAFYPLDAAVTLRFMPGRTLLWPWRLAWFLRRGR
YDVIITVSMGKLSSLMVPYLRLLCPHSRLLLSEHVSFHQYAWPMKWLKVLVYRLGDRTVLLTQKDLATISRWVPERKCQV
IENVSPFPVQSPQTKLQQPVALAVGRLCHQKGFDRLIAAWQRVARQTAGWQLHIVGDGPDRAALQQQIDAAGLAEQVKLL
PATADIAGHYRQAAMLLMTSRYEGLPMVLIEAMSFGLPLVAFDCQTGPAELIDDEGNGYLVAEGDIEAFSRRTLALIDDG
QLRQRFSLRSLERAQQFIPERIYPQWQQLIAGG

Sequences:

>Translated_353_residues
MQQRKVAIVIENIAEKGGTERVASSLANALATRLGHQVELVSISGDRAFYPLDAAVTLRFMPGRTLLWPWRLAWFLRRGR
YDVIITVSMGKLSSLMVPYLRLLCPHSRLLLSEHVSFHQYAWPMKWLKVLVYRLGDRTVLLTQKDLATISRWVPERKCQV
IENVSPFPVQSPQTKLQQPVALAVGRLCHQKGFDRLIAAWQRVARQTAGWQLHIVGDGPDRAALQQQIDAAGLAEQVKLL
PATADIAGHYRQAAMLLMTSRYEGLPMVLIEAMSFGLPLVAFDCQTGPAELIDDEGNGYLVAEGDIEAFSRRTLALIDDG
QLRQRFSLRSLERAQQFIPERIYPQWQQLIAGG
>Mature_353_residues
MQQRKVAIVIENIAEKGGTERVASSLANALATRLGHQVELVSISGDRAFYPLDAAVTLRFMPGRTLLWPWRLAWFLRRGR
YDVIITVSMGKLSSLMVPYLRLLCPHSRLLLSEHVSFHQYAWPMKWLKVLVYRLGDRTVLLTQKDLATISRWVPERKCQV
IENVSPFPVQSPQTKLQQPVALAVGRLCHQKGFDRLIAAWQRVARQTAGWQLHIVGDGPDRAALQQQIDAAGLAEQVKLL
PATADIAGHYRQAAMLLMTSRYEGLPMVLIEAMSFGLPLVAFDCQTGPAELIDDEGNGYLVAEGDIEAFSRRTLALIDDG
QLRQRFSLRSLERAQQFIPERIYPQWQQLIAGG

Specific function: Involved in the biosynthesis of amylovoran which functions as a virulence factor. May be involved in the formation of galactose alpha-1,6 linkages in amylovoran [H]

COG id: COG0438

COG function: function code M; Glycosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI226510984, Length=175, Percent_Identity=30.2857142857143, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788357, Length=149, Percent_Identity=29.5302013422819, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.4.1.- [C]

Molecular weight: Translated: 39732; Mature: 39732

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQRKVAIVIENIAEKGGTERVASSLANALATRLGHQVELVSISGDRAFYPLDAAVTLRF
CCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEECCCEEEEEE
MPGRTLLWPWRLAWFLRRGRYDVIITVSMGKLSSLMVPYLRLLCPHSRLLLSEHVSFHQY
CCCCCEEHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHCHHHHH
AWPMKWLKVLVYRLGDRTVLLTQKDLATISRWVPERKCQVIENVSPFPVQSPQTKLQQPV
HHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHCHH
ALAVGRLCHQKGFDRLIAAWQRVARQTAGWQLHIVGDGPDRAALQQQIDAAGLAEQVKLL
HHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHC
PATADIAGHYRQAAMLLMTSRYEGLPMVLIEAMSFGLPLVAFDCQTGPAELIDDEGNGYL
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHCCCCCCEEE
VAEGDIEAFSRRTLALIDDGQLRQRFSLRSLERAQQFIPERIYPQWQQLIAGG
EECCCHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHCCC
>Mature Secondary Structure
MQQRKVAIVIENIAEKGGTERVASSLANALATRLGHQVELVSISGDRAFYPLDAAVTLRF
CCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEECCCEEEEEE
MPGRTLLWPWRLAWFLRRGRYDVIITVSMGKLSSLMVPYLRLLCPHSRLLLSEHVSFHQY
CCCCCEEHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHCHHHHH
AWPMKWLKVLVYRLGDRTVLLTQKDLATISRWVPERKCQVIENVSPFPVQSPQTKLQQPV
HHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHHCHH
ALAVGRLCHQKGFDRLIAAWQRVARQTAGWQLHIVGDGPDRAALQQQIDAAGLAEQVKLL
HHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHC
PATADIAGHYRQAAMLLMTSRYEGLPMVLIEAMSFGLPLVAFDCQTGPAELIDDEGNGYL
CCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHCCCCCCEEE
VAEGDIEAFSRRTLALIDDGQLRQRFSLRSLERAQQFIPERIYPQWQQLIAGG
EECCCHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Hexosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596293 [H]