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The map label for this gene is narH [H]

Identifier: 157371122

GI number: 157371122

Start: 3160544

End: 3162088

Strand: Reverse

Name: narH [H]

Synonym: Spro_2882

Alternate gene names: 157371122

Gene position: 3162088-3160544 (Counterclockwise)

Preceding gene: 157371123

Following gene: 157371121

Centisome position: 58.03

GC content: 58.64

Gene sequence:

>1545_bases
ATGAAAATTCGTTCGCAAGTTGGCATGGTGCTGAACCTGGACAAATGCATCGGTTGCCACACCTGTTCGGTGACCTGTAA
AAACGTCTGGACCAGCCGCGAGGGCATGGAATACGCCTGGTTCAATAACGTGGAAAGCAAACCCGGCGTCGGTTATCCGC
ACGCCTGGGAAGATCAGGAGAAGTGGAAGGGCGGTTGGATCCGTAAAATCAACGGCAAGCTGGAGCCGCGCATGGGCGGC
CGCGTCGGCCTGTTGTCGAAGATATTCGCCAACCCGGACGTTCCGGCGCTGGACGACTATTACGAGCCGTTCGACTACGA
CTACCAGAACCTGCATACCGCGCCGCAGGGCAAGCATCAGCCGATCGCCCGGCCGCGCTCGCTGATCACCGGCCAGCGTA
TGAAAAAGATTGAAAATGGCCCGAACTGGGAAGAGATCCTCGGTGGCGAATTCTCCAAGCGTTCGAAGGACAAAAACTTC
GACAATATGCAAAAGGAGATGTACGGCCAGTTCGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCT
TAACCCGGCCTGTGTCGCGACCTGCCCGAGCGGGGCGATCTACAAGCGTGGTGAAGACGGCATTGTGCTGATCGATCAGG
ATAAATGCCGCGGCTGGCGCATGTGCCTGACCGGCTGCCCGTACAAAAAGATCTATTTCAACTGGAAAAGCGGCAAGTCC
GAGAAGTGCATCTTCTGCTATCCGCGCATTGAATCCGGCCAGCCGACGGTGTGTTCGGAAACCTGTGTGGGCCGTATCCG
TTATCTGGGCGTGCTGCTGTATGACGCCGACCGCATCGAGCAGGCGGCAGCGGTCGAAAACGACAAGGATCTGTACCAGA
GCCAACTGGATATTTTCCTCGACCCGCACGATCCGGCGGTAATTGCCCAGGCGTTGGCCGACGGCATTCCACAAGGGGTG
ATCGAATCTGCCCAGCAGTCGCCGGTATACAAAATGGCGATGGACTGGAAGCTGGCACTGCCTCTGCACCCGGAATACCG
CACGCTGCCGATGGTGTGGTACGTGCCGCCATTGTCACCGATTCAATCCGCCGCCGATGCCGGTGAGCTGGCGCACAGCG
GCGTATTGCCGGACGTGGAAAGCCTGCGTATCCCGGTGCAATACCTGGCCAACCTGCTGACCGCCGGCGATACCGCTCCG
GTGCTGTTAGCGCTCAAACGCATGCTGGCGATGCGCCACTACAAACGGGCAGAAACCGTTGACGGCGTGCTGGACACCAG
CGCACTGGAACAGGTGGGGCTGAGCGAAGCGCAGGCGCAGGAGATGTACCGCTATCTGGCGATCGCCAACTACGAAGACC
GCTTCGTGATCCCGTCCAGCCATCGCGAACTGGCGCGTGAGGCCTTCCCGGAAAGCAAAGGCTGCGGCTTTAGCTTTGGT
GACGGCTGCCAGGGTAGCGACGGTAAATTCAACCTGTTCAACAGCCGACGCATTGACGCTATTGATGTGACGAAAAAAAC
GCCCCGCCAGGAGGATGCCTCATGA

Upstream 100 bases:

>100_bases
ACCGCGATGAGTTTGTGGTGGTACGCAAGATGAACCGCATCGACTGGTTAGATGGTGAAGGCAACGACGACTCGCAGGGT
AGCCAGCAGGAGAAAGCAAA

Downstream 100 bases:

>100_bases
TCAGCCTGAAAGTGATTGCTCGCCTGCTGGACTACCCGGAGCAGGCGCTGTTTGACCATCAACAGGCGCTGGTGGAGGCG
CTGGAACCGGCCAGCGAACT

Product: nitrate reductase subunit beta

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVESKPGVGYPHAWEDQEKWKGGWIRKINGKLEPRMGG
RVGLLSKIFANPDVPALDDYYEPFDYDYQNLHTAPQGKHQPIARPRSLITGQRMKKIENGPNWEEILGGEFSKRSKDKNF
DNMQKEMYGQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKRGEDGIVLIDQDKCRGWRMCLTGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEQAAAVENDKDLYQSQLDIFLDPHDPAVIAQALADGIPQGV
IESAQQSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELAHSGVLPDVESLRIPVQYLANLLTAGDTAP
VLLALKRMLAMRHYKRAETVDGVLDTSALEQVGLSEAQAQEMYRYLAIANYEDRFVIPSSHRELAREAFPESKGCGFSFG
DGCQGSDGKFNLFNSRRIDAIDVTKKTPRQEDAS

Sequences:

>Translated_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVESKPGVGYPHAWEDQEKWKGGWIRKINGKLEPRMGG
RVGLLSKIFANPDVPALDDYYEPFDYDYQNLHTAPQGKHQPIARPRSLITGQRMKKIENGPNWEEILGGEFSKRSKDKNF
DNMQKEMYGQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKRGEDGIVLIDQDKCRGWRMCLTGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEQAAAVENDKDLYQSQLDIFLDPHDPAVIAQALADGIPQGV
IESAQQSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELAHSGVLPDVESLRIPVQYLANLLTAGDTAP
VLLALKRMLAMRHYKRAETVDGVLDTSALEQVGLSEAQAQEMYRYLAIANYEDRFVIPSSHRELAREAFPESKGCGFSFG
DGCQGSDGKFNLFNSRRIDAIDVTKKTPRQEDAS
>Mature_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVESKPGVGYPHAWEDQEKWKGGWIRKINGKLEPRMGG
RVGLLSKIFANPDVPALDDYYEPFDYDYQNLHTAPQGKHQPIARPRSLITGQRMKKIENGPNWEEILGGEFSKRSKDKNF
DNMQKEMYGQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKRGEDGIVLIDQDKCRGWRMCLTGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEQAAAVENDKDLYQSQLDIFLDPHDPAVIAQALADGIPQGV
IESAQQSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELAHSGVLPDVESLRIPVQYLANLLTAGDTAP
VLLALKRMLAMRHYKRAETVDGVLDTSALEQVGLSEAQAQEMYRYLAIANYEDRFVIPSSHRELAREAFPESKGCGFSFG
DGCQGSDGKFNLFNSRRIDAIDVTKKTPRQEDAS

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=508, Percent_Identity=86.4173228346457, Blast_Score=936, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=512, Percent_Identity=78.3203125, Blast_Score=880, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=132, Percent_Identity=36.3636363636364, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787122, Length=101, Percent_Identity=42.5742574257426, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI2367345, Length=111, Percent_Identity=28.8288288288288, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1789370, Length=99, Percent_Identity=37.3737373737374, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI226510944, Length=131, Percent_Identity=32.0610687022901, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 57963; Mature: 57963

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVESKPGVGYPHAWEDQE
CCCHHHCCCEEEHHHHHCCCEEEEEHHHHHCCCCCCEEHCCCCCCCCCCCCCCCCCCCHH
KWKGGWIRKINGKLEPRMGGRVGLLSKIFANPDVPALDDYYEPFDYDYQNLHTAPQGKHQ
HHCCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCHHHCCCCCCCCCC
PIARPRSLITGQRMKKIENGPNWEEILGGEFSKRSKDKNFDNMQKEMYGQFENTFMMYLP
CCCCHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVATCPSGAIYKRGEDGIVLIDQDKCRGWRMCLTGCPYKKIYFNWKSGKS
HHHHHHCCCCEEEECCCCCEEECCCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEQAAAVENDKDLYQSQLDIFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHHCCCHHHHHHHCCCEEE
DPHDPAVIAQALADGIPQGVIESAQQSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCCH
IQSAADAGELAHSGVLPDVESLRIPVQYLANLLTAGDTAPVLLALKRMLAMRHYKRAETV
HHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
DGVLDTSALEQVGLSEAQAQEMYRYLAIANYEDRFVIPSSHRELAREAFPESKGCGFSFG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHCCCCCCCCCCCC
DGCQGSDGKFNLFNSRRIDAIDVTKKTPRQEDAS
CCCCCCCCCEEECCCCCCCEEECCCCCCCCCCCC
>Mature Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGMEYAWFNNVESKPGVGYPHAWEDQE
CCCHHHCCCEEEHHHHHCCCEEEEEHHHHHCCCCCCEEHCCCCCCCCCCCCCCCCCCCHH
KWKGGWIRKINGKLEPRMGGRVGLLSKIFANPDVPALDDYYEPFDYDYQNLHTAPQGKHQ
HHCCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCHHHCCCCCCCCCC
PIARPRSLITGQRMKKIENGPNWEEILGGEFSKRSKDKNFDNMQKEMYGQFENTFMMYLP
CCCCHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPACVATCPSGAIYKRGEDGIVLIDQDKCRGWRMCLTGCPYKKIYFNWKSGKS
HHHHHHCCCCEEEECCCCCEEECCCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEQAAAVENDKDLYQSQLDIFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHHCCCHHHHHHHCCCEEE
DPHDPAVIAQALADGIPQGVIESAQQSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCCH
IQSAADAGELAHSGVLPDVESLRIPVQYLANLLTAGDTAPVLLALKRMLAMRHYKRAETV
HHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
DGVLDTSALEQVGLSEAQAQEMYRYLAIANYEDRFVIPSSHRELAREAFPESKGCGFSFG
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHCCCCCCCCCCCC
DGCQGSDGKFNLFNSRRIDAIDVTKKTPRQEDAS
CCCCCCCCCEEECCCCCCCEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]