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The map label for this gene is narI [H]

Identifier: 157371120

GI number: 157371120

Start: 3159145

End: 3159822

Strand: Reverse

Name: narI [H]

Synonym: Spro_2880

Alternate gene names: 157371120

Gene position: 3159822-3159145 (Counterclockwise)

Preceding gene: 157371121

Following gene: 157371110

Centisome position: 57.99

GC content: 57.37

Gene sequence:

>678_bases
ATGCAATTTTTAAATCAGTTCTTCTTCGATATTTACCCCTATCTGGCGGGGGCGATTTTCCTGATCGGCAGTTGGCTGCG
TTACGATTACGGCCAATACAGCTGGCGCGCCGGATCCAGCCAGATGCTGGATAAAAAAGGAATGCGACTGGCCTCCAACC
TGTTCCACATCGGCATTTTGGGCATCTTCGCCGGGCACTTCCTCGGCATGCTGACGCCGCACTGGATGTATGAATCCTTC
CTGCCGATCGACGTGAAGCAGAAACTGGCGATGATCGCCGGTGGTGCCTGCGGCGTAATGACGTTGATTGGCGGGGCGCT
GCTGCTGAAACGCCGGTTGACCAACCCGCGGGTGCGGGCGACCAGCAGTTTTGCCGATATCATGATCCTGACGCTGTTGG
TGGTGCAGGTGTGCCTGGGGCTGCTGACCATTCCGTTCTCCGCCCAGCATATGGACGGCAGCGAGATGATGAAGCTGGTG
GGCTGGGCACAGGCAGTGGTGACCTTCCACGCCGGTGCATCAGAACATCTGGTGGGCGTTGCGCCGATTTTTAAACTGCA
TATGGTGCTGGGCATGACGCTGTTCGTGCTGTTCCCGTTCTGTCGCCTGGTGCATATCTGGAGCGTACCGGTGGAATACC
TGACCCGCCGTTACCAACTGGTGCGTAATCGCCGCTAA

Upstream 100 bases:

>100_bases
CGGCACAGCAGGCGGCGCACCAACGGCGCTTTGCCGGTGCGGTAGCCCCGCAATACCTGGACCTGACGGATGCGCTAACC
GGAACCAAAGGACGCTAATC

Downstream 100 bases:

>100_bases
AGAAATTTCCCACCTCTATTACCCTCCATCTGGCGAAACGCACGCTGTCAGATGGGGGTGTTCGTGATACAAACTTTTTC
TTCCCTCAGCCCCAGTTCCG

Product: respiratory nitrate reductase subunit gamma

Products: NA

Alternate protein names: Cytochrome B-NR; Nitrate reductase A subunit gamma; Quinol-nitrate oxidoreductase subunit gamma [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MQFLNQFFFDIYPYLAGAIFLIGSWLRYDYGQYSWRAGSSQMLDKKGMRLASNLFHIGILGIFAGHFLGMLTPHWMYESF
LPIDVKQKLAMIAGGACGVMTLIGGALLLKRRLTNPRVRATSSFADIMILTLLVVQVCLGLLTIPFSAQHMDGSEMMKLV
GWAQAVVTFHAGASEHLVGVAPIFKLHMVLGMTLFVLFPFCRLVHIWSVPVEYLTRRYQLVRNRR

Sequences:

>Translated_225_residues
MQFLNQFFFDIYPYLAGAIFLIGSWLRYDYGQYSWRAGSSQMLDKKGMRLASNLFHIGILGIFAGHFLGMLTPHWMYESF
LPIDVKQKLAMIAGGACGVMTLIGGALLLKRRLTNPRVRATSSFADIMILTLLVVQVCLGLLTIPFSAQHMDGSEMMKLV
GWAQAVVTFHAGASEHLVGVAPIFKLHMVLGMTLFVLFPFCRLVHIWSVPVEYLTRRYQLVRNRR
>Mature_225_residues
MQFLNQFFFDIYPYLAGAIFLIGSWLRYDYGQYSWRAGSSQMLDKKGMRLASNLFHIGILGIFAGHFLGMLTPHWMYESF
LPIDVKQKLAMIAGGACGVMTLIGGALLLKRRLTNPRVRATSSFADIMILTLLVVQVCLGLLTIPFSAQHMDGSEMMKLV
GWAQAVVTFHAGASEHLVGVAPIFKLHMVLGMTLFVLFPFCRLVHIWSVPVEYLTRRYQLVRNRR

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit [

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=224, Percent_Identity=82.1428571428571, Blast_Score=385, Evalue=1e-109,
Organism=Escherichia coli, GI1787738, Length=225, Percent_Identity=69.7777777777778, Blast_Score=336, Evalue=8e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 25438; Mature: 25438

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFLNQFFFDIYPYLAGAIFLIGSWLRYDYGQYSWRAGSSQMLDKKGMRLASNLFHIGIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GIFAGHFLGMLTPHWMYESFLPIDVKQKLAMIAGGACGVMTLIGGALLLKRRLTNPRVRA
HHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHH
TSSFADIMILTLLVVQVCLGLLTIPFSAQHMDGSEMMKLVGWAQAVVTFHAGASEHLVGV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
APIFKLHMVLGMTLFVLFPFCRLVHIWSVPVEYLTRRYQLVRNRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQFLNQFFFDIYPYLAGAIFLIGSWLRYDYGQYSWRAGSSQMLDKKGMRLASNLFHIGIL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
GIFAGHFLGMLTPHWMYESFLPIDVKQKLAMIAGGACGVMTLIGGALLLKRRLTNPRVRA
HHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHH
TSSFADIMILTLLVVQVCLGLLTIPFSAQHMDGSEMMKLVGWAQAVVTFHAGASEHLVGV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
APIFKLHMVLGMTLFVLFPFCRLVHIWSVPVEYLTRRYQLVRNRR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2832376; 8905232; 9278503; 3053688; 12910261 [H]