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The map label for this gene is ybiK [C]

Identifier: 157371091

GI number: 157371091

Start: 3129717

End: 3130643

Strand: Reverse

Name: ybiK [C]

Synonym: Spro_2851

Alternate gene names: 157371091

Gene position: 3130643-3129717 (Counterclockwise)

Preceding gene: 157371093

Following gene: 157371084

Centisome position: 57.46

GC content: 60.52

Gene sequence:

>927_bases
ATGATTGTGATTGCAAACTCTGAAGGACATCCGGGCATCGGTCTGTCGGCCGAACGGCTGCAGCAGGGGCACAACGGCCT
GCGCGCCATCGTTGAGGGCATCAAGCTGGTGGAGTTGGATCCGAGCGTGCGCACCGTGGGTTATGGCGGCTGGCCCAACG
TATTGGGTGAGATGGAGCTGGATGCCTCGGTGATGGACGGCGATAGCTTGCGCACCGGCGCGGTAGGGGCATTGCAAGGA
TACCTGCATCCGGTGGAGGTGGCGTACCGGGTGATGGAAGACCTTAACCACGAGATCCTGGTTGGCGCCGGGGCGGCACG
TTTTGCCGCTGAAATTGGCGCACTGGCCGGTGACAATCTGATTGCCGACTCCAAACGCGTTTGGTGGGAAAAGCTCGAGC
AGGCGATGAGTGCCGAACAGCGTCGTGACTGGCCGAATATCCCGCTGGCCGGGCTGTCGAACAACGCTACCGATCCGGAG
CGAGTGCGCGATACCACGGTATTTTTAGCGCAAGATAGCGGGGGCACCCTCAGTGCCGCTACCTCTACCTCGGGCTGGGC
GTGGAAATACCCTGGGCGGTTGGGAGACAGTCCGATCATCGGCAGCGGCTCCTATGCCGACAGCCGTTATGGCGCCTGCG
CCTGCACTCACACCGGGGAAATGACCATCCGCTGCGGCACCAGCCGCGCCGTAGTGCTGTACATGAAGATGGGCATGACG
CTGGATCAGGCGGTGTATGAAGCGATCGAGGATTTGAACTACCTGAAGGACGGTTATACCGAAGGGGTGACAATCCATGC
CGTCGATAAGCAGGGCAACCACAAGGTGGTCAGCCTCAATTGCCCCGGCCCGATCCAATACTGGCTGTGGCAAACCGGCA
TGGTAGCACCGGAGTTACGCGATGCCGAGATTATTACTACCCGCTGA

Upstream 100 bases:

>100_bases
CTTTTTAAACACCAATGGAACACGTGTTCCATTGGTGCCGAACACCGGATTATCCGGTGCCTCTTTTGGGCGCTTAACGC
CGTGCGGGAAACAGAACCCT

Downstream 100 bases:

>100_bases
AAGGCGGGGTTAGCAGGAGGCACGCACCACCAGTTCGGTGGGGATGGTCACCTGCTGCGGCGCGGCCTGCGGGCTGCTGC
TGCGCAGTTCGATCAGTTGC

Product: asparaginase

Products: NA

Alternate protein names: Aspartylglucosaminidase; AGA; Glycosylasparaginase; N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MIVIANSEGHPGIGLSAERLQQGHNGLRAIVEGIKLVELDPSVRTVGYGGWPNVLGEMELDASVMDGDSLRTGAVGALQG
YLHPVEVAYRVMEDLNHEILVGAGAARFAAEIGALAGDNLIADSKRVWWEKLEQAMSAEQRRDWPNIPLAGLSNNATDPE
RVRDTTVFLAQDSGGTLSAATSTSGWAWKYPGRLGDSPIIGSGSYADSRYGACACTHTGEMTIRCGTSRAVVLYMKMGMT
LDQAVYEAIEDLNYLKDGYTEGVTIHAVDKQGNHKVVSLNCPGPIQYWLWQTGMVAPELRDAEIITTR

Sequences:

>Translated_308_residues
MIVIANSEGHPGIGLSAERLQQGHNGLRAIVEGIKLVELDPSVRTVGYGGWPNVLGEMELDASVMDGDSLRTGAVGALQG
YLHPVEVAYRVMEDLNHEILVGAGAARFAAEIGALAGDNLIADSKRVWWEKLEQAMSAEQRRDWPNIPLAGLSNNATDPE
RVRDTTVFLAQDSGGTLSAATSTSGWAWKYPGRLGDSPIIGSGSYADSRYGACACTHTGEMTIRCGTSRAVVLYMKMGMT
LDQAVYEAIEDLNYLKDGYTEGVTIHAVDKQGNHKVVSLNCPGPIQYWLWQTGMVAPELRDAEIITTR
>Mature_308_residues
MIVIANSEGHPGIGLSAERLQQGHNGLRAIVEGIKLVELDPSVRTVGYGGWPNVLGEMELDASVMDGDSLRTGAVGALQG
YLHPVEVAYRVMEDLNHEILVGAGAARFAAEIGALAGDNLIADSKRVWWEKLEQAMSAEQRRDWPNIPLAGLSNNATDPE
RVRDTTVFLAQDSGGTLSAATSTSGWAWKYPGRLGDSPIIGSGSYADSRYGACACTHTGEMTIRCGTSRAVVLYMKMGMT
LDQAVYEAIEDLNYLKDGYTEGVTIHAVDKQGNHKVVSLNCPGPIQYWLWQTGMVAPELRDAEIITTR

Specific function: Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins. Requires that the glycosylated asparagine moiety is not substituted on its N- (R1) and C- (R2) terminus [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=239, Percent_Identity=33.0543933054393, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI145275200, Length=239, Percent_Identity=33.0543933054393, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI285002251, Length=236, Percent_Identity=33.4745762711864, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI285002253, Length=236, Percent_Identity=31.7796610169492, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI98991769, Length=206, Percent_Identity=32.5242718446602, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1787050, Length=275, Percent_Identity=32.3636363636364, Blast_Score=104, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17569239, Length=248, Percent_Identity=31.4516129032258, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI221330102, Length=281, Percent_Identity=35.5871886120996, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24652135, Length=281, Percent_Identity=35.5871886120996, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI18921183, Length=228, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24655553, Length=252, Percent_Identity=32.1428571428571, Blast_Score=108, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.5.1.26 [H]

Molecular weight: Translated: 33236; Mature: 33236

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVIANSEGHPGIGLSAERLQQGHNGLRAIVEGIKLVELDPSVRTVGYGGWPNVLGEMEL
CEEEECCCCCCCCCCCHHHHHHCCHHHHHHHCCCEEEEECCCEEEEECCCCHHHCCCCCC
DASVMDGDSLRTGAVGALQGYLHPVEVAYRVMEDLNHEILVGAGAARFAAEIGALAGDNL
CCEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCCCE
IADSKRVWWEKLEQAMSAEQRRDWPNIPLAGLSNNATDPERVRDTTVFLAQDSGGTLSAA
ECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCEEEEEEEECCCCEEEEE
TSTSGWAWKYPGRLGDSPIIGSGSYADSRYGACACTHTGEMTIRCGTSRAVVLYMKMGMT
CCCCCCEEECCCCCCCCCEECCCCCCCCCCCEEEEECCCCEEEEECCCCEEEEEEECCCC
LDQAVYEAIEDLNYLKDGYTEGVTIHAVDKQGNHKVVSLNCPGPIQYWLWQTGMVAPELR
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCHHEHEEECCCCCCCCC
DAEIITTR
CCEEEECC
>Mature Secondary Structure
MIVIANSEGHPGIGLSAERLQQGHNGLRAIVEGIKLVELDPSVRTVGYGGWPNVLGEMEL
CEEEECCCCCCCCCCCHHHHHHCCHHHHHHHCCCEEEEECCCEEEEECCCCHHHCCCCCC
DASVMDGDSLRTGAVGALQGYLHPVEVAYRVMEDLNHEILVGAGAARFAAEIGALAGDNL
CCEEECCCCCCCCCHHHHHHCCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHCCCCE
IADSKRVWWEKLEQAMSAEQRRDWPNIPLAGLSNNATDPERVRDTTVFLAQDSGGTLSAA
ECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCEEEEEEEECCCCEEEEE
TSTSGWAWKYPGRLGDSPIIGSGSYADSRYGACACTHTGEMTIRCGTSRAVVLYMKMGMT
CCCCCCEEECCCCCCCCCEECCCCCCCCCCCEEEEECCCCEEEEECCCCEEEEEEECCCC
LDQAVYEAIEDLNYLKDGYTEGVTIHAVDKQGNHKVVSLNCPGPIQYWLWQTGMVAPELR
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEECCCCHHEHEEECCCCCCCCC
DAEIITTR
CCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7840643; 8250923; 9541410 [H]