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The map label for this gene is manX [H]

Identifier: 157371053

GI number: 157371053

Start: 3094564

End: 3095535

Strand: Direct

Name: manX [H]

Synonym: Spro_2813

Alternate gene names: 157371053

Gene position: 3094564-3095535 (Clockwise)

Preceding gene: 157371051

Following gene: 157371054

Centisome position: 56.79

GC content: 55.35

Gene sequence:

>972_bases
GTGGCAATAGCTATTATCATCGGCACACACGGGACCGCAGCAGAACAATTGCTGAAGACCACGGAAATGCTATTGGGCGA
ACAGGATAACGTCGCTTTTATAGATTTCGTCCCCGGTGAGAATGCCGAAACGTTAATCGAGAAGTACAAGGGTAAAATCA
GCGGACTAGACACCAGCGGTGGCGTCCTGTTCCTGGTGGATACCTGGGGCGGCAGCCCGTTCAACGCGGCCAGCCGCATC
GCCGTCGATAAAGAGAACTATGAGGTCGTCACCGGGGTTAACATTCCGATGCTGGTGGAAACGTTCATGGCACGAGACGA
CAACCCTGGCTTCGACGAACTGGTCGCGCTGGCGTTGGAAACCGGCCGTGAAGGCGTGAAAGCGCTGAAAAAACCGGCCG
AAGAGAGTGTTAAGCCCGCGGCACCACAAAGTGCTCCCACCGCTAAACCCGCCGCCCCTCAGGCGCCGCTGGGTCCCAAT
GACCATATGAAAATCGGCCTGGCCCGTATCGATGACCGTTTGATCCACGGTCAGGTCGCGACCCGTTGGACCAAGGAAAC
CGACGTCAGCCGTATCATCGTGATCAGCGACGAAGTCGCCGCCGACCACGTTCGCAAAACCTTGCTGACTCAGGTTGCCC
CGCCGGGCGTCACCGCACACGTGGTCGACGTGGCGAAAGCCATCCGCGTGTGGAACAACCCGAAATATGCCGGCGATCGC
GTGATGCTGCTGTTCACCAATCCGACCGATGTCTGGCGTTTGGTGGAAGCCGGCGTGGATATCAAATCGGTGAATATCGG
TGGTATGGCGTTCCGTCAGGGCAAAACCCAGGTGAACAACGCCGTCTCGGTTGACGAAAAAGATATCGAAGCGTTTAAGA
AATTAAACGAGCGCGGTATTGAATTGGAAGTTCGTAAAGTCTCGTCCGACAGTCGGTTAAAAATGATGGACCTGATTAAC
AAACTCAATTAA

Upstream 100 bases:

>100_bases
TTCTACCATAATGTATCTGAACCACGGAAGTGACCGCCAGCAATAAAATGAACTGAACCAACACACCATCCGTGCATGAA
GTTCTATGAGGAGGTAGCGA

Downstream 100 bases:

>100_bases
TGGCGACAGCCAATAATTATTTTACTCAGAGATCTTTGGTCATAGGAGAAGTGCAATGGAGATTACCACTCTTCAGATTG
TACTGATATTTATCGTTGCC

Product: PTS system mannose/fructose/sorbose family transporter subunit IIB

Products: NA

Alternate protein names: EIIAB-Man; Mannose-specific phosphotransferase enzyme IIA component; EIII-Man; PTS system mannose-specific EIIA component; Mannose-specific phosphotransferase enzyme IIB component; PTS system mannose-specific EIIB component [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MAIAIIIGTHGTAAEQLLKTTEMLLGEQDNVAFIDFVPGENAETLIEKYKGKISGLDTSGGVLFLVDTWGGSPFNAASRI
AVDKENYEVVTGVNIPMLVETFMARDDNPGFDELVALALETGREGVKALKKPAEESVKPAAPQSAPTAKPAAPQAPLGPN
DHMKIGLARIDDRLIHGQVATRWTKETDVSRIIVISDEVAADHVRKTLLTQVAPPGVTAHVVDVAKAIRVWNNPKYAGDR
VMLLFTNPTDVWRLVEAGVDIKSVNIGGMAFRQGKTQVNNAVSVDEKDIEAFKKLNERGIELEVRKVSSDSRLKMMDLIN
KLN

Sequences:

>Translated_323_residues
MAIAIIIGTHGTAAEQLLKTTEMLLGEQDNVAFIDFVPGENAETLIEKYKGKISGLDTSGGVLFLVDTWGGSPFNAASRI
AVDKENYEVVTGVNIPMLVETFMARDDNPGFDELVALALETGREGVKALKKPAEESVKPAAPQSAPTAKPAAPQAPLGPN
DHMKIGLARIDDRLIHGQVATRWTKETDVSRIIVISDEVAADHVRKTLLTQVAPPGVTAHVVDVAKAIRVWNNPKYAGDR
VMLLFTNPTDVWRLVEAGVDIKSVNIGGMAFRQGKTQVNNAVSVDEKDIEAFKKLNERGIELEVRKVSSDSRLKMMDLIN
KLN
>Mature_322_residues
AIAIIIGTHGTAAEQLLKTTEMLLGEQDNVAFIDFVPGENAETLIEKYKGKISGLDTSGGVLFLVDTWGGSPFNAASRIA
VDKENYEVVTGVNIPMLVETFMARDDNPGFDELVALALETGREGVKALKKPAEESVKPAAPQSAPTAKPAAPQAPLGPND
HMKIGLARIDDRLIHGQVATRWTKETDVSRIIVISDEVAADHVRKTLLTQVAPPGVTAHVVDVAKAIRVWNNPKYAGDRV
MLLFTNPTDVWRLVEAGVDIKSVNIGGMAFRQGKTQVNNAVSVDEKDIEAFKKLNERGIELEVRKVSSDSRLKMMDLINK
LN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG3444

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIB type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788120, Length=324, Percent_Identity=80.2469135802469, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI87082217, Length=157, Percent_Identity=35.6687898089172, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789527, Length=154, Percent_Identity=32.4675324675325, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701
- InterPro:   IPR013789
- InterPro:   IPR004720
- InterPro:   IPR018455 [H]

Pfam domain/function: PF03610 EIIA-man; PF03830 PTSIIB_sorb [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 35042; Mature: 34910

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS51096 PTS_EIIA_TYPE_4 ; PS51101 PTS_EIIB_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIAIIIGTHGTAAEQLLKTTEMLLGEQDNVAFIDFVPGENAETLIEKYKGKISGLDTSG
CEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCC
GVLFLVDTWGGSPFNAASRIAVDKENYEVVTGVNIPMLVETFMARDDNPGFDELVALALE
CEEEEEECCCCCCCCCCCEEEECCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHH
TGREGVKALKKPAEESVKPAAPQSAPTAKPAAPQAPLGPNDHMKIGLARIDDRLIHGQVA
HCHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHCCHHHCCHHH
TRWTKETDVSRIIVISDEVAADHVRKTLLTQVAPPGVTAHVVDVAKAIRVWNNPKYAGDR
HCCCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCE
VMLLFTNPTDVWRLVEAGVDIKSVNIGGMAFRQGKTQVNNAVSVDEKDIEAFKKLNERGI
EEEEEECCHHHHHHHHCCCCEEEEECCCEEEECCCHHHCCCCCCCHHHHHHHHHHHHCCC
ELEVRKVSSDSRLKMMDLINKLN
EEEEEECCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AIAIIIGTHGTAAEQLLKTTEMLLGEQDNVAFIDFVPGENAETLIEKYKGKISGLDTSG
EEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCCCCCCC
GVLFLVDTWGGSPFNAASRIAVDKENYEVVTGVNIPMLVETFMARDDNPGFDELVALALE
CEEEEEECCCCCCCCCCCEEEECCCCCEEEECCCHHHHHHHHHHCCCCCCHHHHHHHHHH
TGREGVKALKKPAEESVKPAAPQSAPTAKPAAPQAPLGPNDHMKIGLARIDDRLIHGQVA
HCHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHCCHHHCCHHH
TRWTKETDVSRIIVISDEVAADHVRKTLLTQVAPPGVTAHVVDVAKAIRVWNNPKYAGDR
HCCCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCE
VMLLFTNPTDVWRLVEAGVDIKSVNIGGMAFRQGKTQVNNAVSVDEKDIEAFKKLNERGI
EEEEEECCHHHHHHHHCCCCEEEEECCCEEEECCCHHHCCCCCCCHHHHHHHHHHHHCCC
ELEVRKVSSDSRLKMMDLINKLN
EEEEEECCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]