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The map label for this gene is rffG [C]

Identifier: 157370947

GI number: 157370947

Start: 2983321

End: 2984331

Strand: Reverse

Name: rffG [C]

Synonym: Spro_2707

Alternate gene names: 157370947

Gene position: 2984331-2983321 (Counterclockwise)

Preceding gene: 157370948

Following gene: 157370945

Centisome position: 54.77

GC content: 49.26

Gene sequence:

>1011_bases
ATGAAATTCCTGGTTACTGGCGCTGCAGGTTTTATTGGTTTTCACGTTGCTGAACGTCTGTTGGCCGCAGGGCATCAGGT
TGTCGGTATTGACAACCTGAATGATTACTACGACGTAGGTTTGAAAATAGCGCGTCTCGATTTGCTGGCGGATAAGCCTG
CCTTCCAGTTTATTAAGCTGGATTTGGCCGACCGTGAGGGCATGGCGCAGCTGTTTGCCGAGCATCAGTTTCAACGGGTG
ATTCATCTTGGTGCGCAAGCCGGGGTGCGTTATTCATTGGACAACCCGCTGGCGTATGCAGACTCGAATCTTATCGGGCA
TTTGAATGTGCTGGAAGGGTGCCGACATAATAAAGTTGAGCATTTATTATATGCCTCATCCAGTTCGGTATATGGTCTAA
ATCGTAAGTTACCCTTTGCGACCGAAGATTCTGTTGACCACCCGGTATCATTGTATGCGGCGACTAAGAAAGCCAATGAA
CTGATGTCACACAGCTACTCCCACTTATACGGTCTGCCAACCACCGGGCTGCGTTTCTTTACCGTTTATGGCCCTTGGGG
GCGCCCGGATATGGCGCTGTTTAAATTTACCAAGGCCATATTGGCGGGTGAAAGCATCGATGTATATAACCACGGTGAGA
TGCACCGTGACTTTACGTACATCGACGATATCGCTGAGGCTATTGTGCGGTTGCAGGCAGTTATTCCTCAGGCAAATGCT
AGCTGGACGGTTGAGCAGGGTTCACCGGCGACCAGTTCTGCGCCATACCATGTCTATAATATCGGCAACAGCAGCCCGGT
AAAGCTGATGGAATATATCAGCGCACTGGAACAGGCGTTGGGGATCGAGGCGCGTAAGAATATGCTGCCGATGCAGCCTG
GTGACGTGTTAGACACCAGCGCTGATACTGTGGATCTTTATCGTGAGATTGGCTTCAAGCCTGAAACCAGCGTTGAAGAA
GGTGTGAAACGCTTTGTTGAATGGTATAAGTCTTTTTATAAAGTTCAGTAA

Upstream 100 bases:

>100_bases
TCGTAACCTGTTTGATCCTGAGCGCCTGGAAAGCCGTGGCTTTACCTATTATGCGATTGGTCGCGGTGCGTCCATTCAGC
CGGTTATATAAGGGGCATAT

Downstream 100 bases:

>100_bases
TTGAGCTGTACGTAAATGCTATAAGGGGCCACGGCCCCTTTTTTATGCTTTGCAAAAGTCAGGAAGGGGCAGCTAGCCGA
GAAGCAAGACCAATGAGGTA

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKFLVTGAAGFIGFHVAERLLAAGHQVVGIDNLNDYYDVGLKIARLDLLADKPAFQFIKLDLADREGMAQLFAEHQFQRV
IHLGAQAGVRYSLDNPLAYADSNLIGHLNVLEGCRHNKVEHLLYASSSSVYGLNRKLPFATEDSVDHPVSLYAATKKANE
LMSHSYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAILAGESIDVYNHGEMHRDFTYIDDIAEAIVRLQAVIPQANA
SWTVEQGSPATSSAPYHVYNIGNSSPVKLMEYISALEQALGIEARKNMLPMQPGDVLDTSADTVDLYREIGFKPETSVEE
GVKRFVEWYKSFYKVQ

Sequences:

>Translated_336_residues
MKFLVTGAAGFIGFHVAERLLAAGHQVVGIDNLNDYYDVGLKIARLDLLADKPAFQFIKLDLADREGMAQLFAEHQFQRV
IHLGAQAGVRYSLDNPLAYADSNLIGHLNVLEGCRHNKVEHLLYASSSSVYGLNRKLPFATEDSVDHPVSLYAATKKANE
LMSHSYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAILAGESIDVYNHGEMHRDFTYIDDIAEAIVRLQAVIPQANA
SWTVEQGSPATSSAPYHVYNIGNSSPVKLMEYISALEQALGIEARKNMLPMQPGDVLDTSADTVDLYREIGFKPETSVEE
GVKRFVEWYKSFYKVQ
>Mature_336_residues
MKFLVTGAAGFIGFHVAERLLAAGHQVVGIDNLNDYYDVGLKIARLDLLADKPAFQFIKLDLADREGMAQLFAEHQFQRV
IHLGAQAGVRYSLDNPLAYADSNLIGHLNVLEGCRHNKVEHLLYASSSSVYGLNRKLPFATEDSVDHPVSLYAATKKANE
LMSHSYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAILAGESIDVYNHGEMHRDFTYIDDIAEAIVRLQAVIPQANA
SWTVEQGSPATSSAPYHVYNIGNSSPVKLMEYISALEQALGIEARKNMLPMQPGDVLDTSADTVDLYREIGFKPETSVEE
GVKRFVEWYKSFYKVQ

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=338, Percent_Identity=24.8520710059172, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI56237023, Length=353, Percent_Identity=24.6458923512748, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI56118217, Length=353, Percent_Identity=24.6458923512748, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI189083684, Length=353, Percent_Identity=24.6458923512748, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI42516563, Length=345, Percent_Identity=25.5072463768116, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI48994969, Length=359, Percent_Identity=27.5766016713092, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1788353, Length=361, Percent_Identity=27.4238227146814, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1786974, Length=349, Percent_Identity=24.0687679083095, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788366, Length=351, Percent_Identity=24.5014245014245, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71982035, Length=352, Percent_Identity=25.8522727272727, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71982038, Length=354, Percent_Identity=25.7062146892655, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17568069, Length=236, Percent_Identity=27.5423728813559, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=340, Percent_Identity=23.5294117647059, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17539424, Length=243, Percent_Identity=25.9259259259259, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901786, Length=243, Percent_Identity=25.9259259259259, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=243, Percent_Identity=25.9259259259259, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=243, Percent_Identity=25.9259259259259, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=243, Percent_Identity=25.9259259259259, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115532424, Length=339, Percent_Identity=20.9439528023599, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=350, Percent_Identity=27.7142857142857, Blast_Score=103, Evalue=4e-23,
Organism=Drosophila melanogaster, GI19923002, Length=348, Percent_Identity=27.5862068965517, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21356223, Length=342, Percent_Identity=25.4385964912281, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37443; Mature: 37443

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLVTGAAGFIGFHVAERLLAAGHQVVGIDNLNDYYDVGLKIARLDLLADKPAFQFIKL
CEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHCEEEEEEEHHCCCCCEEEEEE
DLADREGMAQLFAEHQFQRVIHLGAQAGVRYSLDNPLAYADSNLIGHLNVLEGCRHNKVE
ECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEECCCCCEEHHHHHHHHCCCCHH
HLLYASSSSVYGLNRKLPFATEDSVDHPVSLYAATKKANELMSHSYSHLYGLPTTGLRFF
HHEEECCCCEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
TVYGPWGRPDMALFKFTKAILAGESIDVYNHGEMHRDFTYIDDIAEAIVRLQAVIPQANA
EEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
SWTVEQGSPATSSAPYHVYNIGNSSPVKLMEYISALEQALGIEARKNMLPMQPGDVLDTS
CEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCC
ADTVDLYREIGFKPETSVEEGVKRFVEWYKSFYKVQ
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFLVTGAAGFIGFHVAERLLAAGHQVVGIDNLNDYYDVGLKIARLDLLADKPAFQFIKL
CEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHCEEEEEEEHHCCCCCEEEEEE
DLADREGMAQLFAEHQFQRVIHLGAQAGVRYSLDNPLAYADSNLIGHLNVLEGCRHNKVE
ECCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCEECCCCCEEHHHHHHHHCCCCHH
HLLYASSSSVYGLNRKLPFATEDSVDHPVSLYAATKKANELMSHSYSHLYGLPTTGLRFF
HHEEECCCCEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
TVYGPWGRPDMALFKFTKAILAGESIDVYNHGEMHRDFTYIDDIAEAIVRLQAVIPQANA
EEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
SWTVEQGSPATSSAPYHVYNIGNSSPVKLMEYISALEQALGIEARKNMLPMQPGDVLDTS
CEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCC
ADTVDLYREIGFKPETSVEEGVKRFVEWYKSFYKVQ
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]