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The map label for this gene is rluB [H]

Identifier: 157370902

GI number: 157370902

Start: 2932342

End: 2933241

Strand: Reverse

Name: rluB [H]

Synonym: Spro_2662

Alternate gene names: 157370902

Gene position: 2933241-2932342 (Counterclockwise)

Preceding gene: 157370903

Following gene: 157370899

Centisome position: 53.83

GC content: 56.67

Gene sequence:

>900_bases
ATGAGCGAGAAGTTACAGAAAGTTTTAGCGCGCGCCGGCCATGGCTCGCGTCGTGAAATCGAAACCATTATTGAAGCCGG
CCGCGTCAGCGTCGACGGTAAAATCGCCAAATTGGGTGATCGCGTTGAAGTCACCGCTTCAATGAAAATCCGTTTGGATG
GCCACGTTGTCTCAATTAAAGAATCCGAGGAAGAAGTTTGCCGTGTGCTGGCGTATTACAAGCCGGAAGGTGAACTCTGC
ACCCGTAGCGATCCTGAAGGCCGCCCGACGGTGTTTGATCGCCTGCCTAAGCTGCGTGGCTCACGCTGGGTAGCGGTAGG
GCGTCTGGACGTCAATACATCAGGCTTGCTGCTGTTCACCACCGACGGTGAATTGGCGAACCGCCTGATGCACCCGAGCC
GTGAAGTTGAACGTGAATATGCGGTGCGTGTATTTGGTCAGATTGATGACGAAAAAATCAAGCAACTGAGCAAGGGCGTT
CAGCTGGAAGACGGCCCGGCAGCTTTCCGCACCATCAGCTTCCAGGGTGGCGAAGGCATCAACCAGTGGTACAACGTAAC
CCTGACCGAAGGGCGTAACCGTGAGGTTCGTCGTCTTTGGGAAGCGGTTGGCGTTCAGGTTAGTCGCCTGATCCGCGTAC
GCTACGGCGATATCGATCTGCCTAAGGGCCTGCCGCGTGGCGGCTGGACTGAACTGGATTTGCCGGCGATTAACTATCTG
CGTGAACTGGTTGAATTGAAGCCGGAAACCGTTAGCAAGATGCCGATTGAGCGCGAACGTCGCCGCGTGAAAGCCAATCA
GATCCGCCGTGCCGTCAAGCGCCATACCCAGGTTGCCGGCAGCGGTCGCCGCAGCACTGCGGGCAGCAAGCCTGGCAACA
AACCTAGCAAGCGCAGCTAA

Upstream 100 bases:

>100_bases
CGCGGTTCGATCTATGTAAACGTATTGTTACTGCTGCGGCGCCTGACGCCGCGGCAGTCATCCCCCCGACGCCTGTGAAG
GCGACACTAGAGGTTGCTCA

Downstream 100 bases:

>100_bases
TCATCACGCCGCAACCCTCTTCGGGCTGCGGCGTTTTCTTTCTGTTACCAGTCAATTCCCTGTTGCGCCTTGATGCCCGC
GTCGAACGCGTGTTTCACCG

Product: 23S rRNA pseudouridylate synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSEKLQKVLARAGHGSRREIETIIEAGRVSVDGKIAKLGDRVEVTASMKIRLDGHVVSIKESEEEVCRVLAYYKPEGELC
TRSDPEGRPTVFDRLPKLRGSRWVAVGRLDVNTSGLLLFTTDGELANRLMHPSREVEREYAVRVFGQIDDEKIKQLSKGV
QLEDGPAAFRTISFQGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIDLPKGLPRGGWTELDLPAINYL
RELVELKPETVSKMPIERERRRVKANQIRRAVKRHTQVAGSGRRSTAGSKPGNKPSKRS

Sequences:

>Translated_299_residues
MSEKLQKVLARAGHGSRREIETIIEAGRVSVDGKIAKLGDRVEVTASMKIRLDGHVVSIKESEEEVCRVLAYYKPEGELC
TRSDPEGRPTVFDRLPKLRGSRWVAVGRLDVNTSGLLLFTTDGELANRLMHPSREVEREYAVRVFGQIDDEKIKQLSKGV
QLEDGPAAFRTISFQGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIDLPKGLPRGGWTELDLPAINYL
RELVELKPETVSKMPIERERRRVKANQIRRAVKRHTQVAGSGRRSTAGSKPGNKPSKRS
>Mature_298_residues
SEKLQKVLARAGHGSRREIETIIEAGRVSVDGKIAKLGDRVEVTASMKIRLDGHVVSIKESEEEVCRVLAYYKPEGELCT
RSDPEGRPTVFDRLPKLRGSRWVAVGRLDVNTSGLLLFTTDGELANRLMHPSREVEREYAVRVFGQIDDEKIKQLSKGVQ
LEDGPAAFRTISFQGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIDLPKGLPRGGWTELDLPAINYLR
ELVELKPETVSKMPIERERRRVKANQIRRAVKRHTQVAGSGRRSTAGSKPGNKPSKRS

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=292, Percent_Identity=84.2465753424658, Blast_Score=506, Evalue=1e-145,
Organism=Escherichia coli, GI1790453, Length=311, Percent_Identity=29.2604501607717, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=30.3418803418803, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081838, Length=193, Percent_Identity=32.6424870466321, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 33703; Mature: 33572

Theoretical pI: Translated: 10.58; Mature: 10.58

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLQKVLARAGHGSRREIETIIEAGRVSVDGKIAKLGDRVEVTASMKIRLDGHVVSIK
CCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHCCCEEEEEEEEEEEECCEEEEEE
ESEEEVCRVLAYYKPEGELCTRSDPEGRPTVFDRLPKLRGSRWVAVGRLDVNTSGLLLFT
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEEEECCCCEEEEE
TDGELANRLMHPSREVEREYAVRVFGQIDDEKIKQLSKGVQLEDGPAAFRTISFQGGEGI
CCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCC
NQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIDLPKGLPRGGWTELDLPAINYL
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHH
RELVELKPETVSKMPIERERRRVKANQIRRAVKRHTQVAGSGRRSTAGSKPGNKPSKRS
HHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEKLQKVLARAGHGSRREIETIIEAGRVSVDGKIAKLGDRVEVTASMKIRLDGHVVSIK
CHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHCCCEEEEEEEEEEEECCEEEEEE
ESEEEVCRVLAYYKPEGELCTRSDPEGRPTVFDRLPKLRGSRWVAVGRLDVNTSGLLLFT
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEEEECCCCEEEEE
TDGELANRLMHPSREVEREYAVRVFGQIDDEKIKQLSKGVQLEDGPAAFRTISFQGGEGI
CCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCC
NQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDIDLPKGLPRGGWTELDLPAINYL
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCHHHHHH
RELVELKPETVSKMPIERERRRVKANQIRRAVKRHTQVAGSGRRSTAGSKPGNKPSKRS
HHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]