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The map label for this gene is yjiA [C]

Identifier: 157370866

GI number: 157370866

Start: 2889759

End: 2890946

Strand: Reverse

Name: yjiA [C]

Synonym: Spro_2626

Alternate gene names: 157370866

Gene position: 2890946-2889759 (Counterclockwise)

Preceding gene: 157370867

Following gene: 157370864

Centisome position: 53.06

GC content: 58.16

Gene sequence:

>1188_bases
ATGAATCCATTACCTGTAACCGTACTTTCCGGCTTTCTCGGCGCCGGTAAAACCACGGTGCTGAACCATATTCTGAATAA
TCGTCAGGGCATGCGTGTGGCGGTGATCGTTAACGACATGAGCGAAGTGAACATTGACGCCGCGCTGGTCGAGAACGAAG
TCCAGCTCGACCGCCAACAGGAAAAGCTGGTGGAGATGAGCAATGGCTGCATCTGCTGCACGCTGCGTGAAGACCTGCTG
ATTTCGGTGCGCGAACTGGCGCAGGCCGGCCGCTTTGATTACCTGCTGATCGAGTCCAGCGGTATCTCCGAACCTTTACC
GGTGGCAGAAACCTTCACCTTTGAAGACGAGCAGGGCGAAAGCCTGTCGCACTTCGCCCGCCTGGACACCCTGGTTACGG
TGGTGGACGGAGTCAACTTCATCCAGCAGTACCAACAGGCGCAAACGCTGCAGGAAGCCGGTCAAAGCCTGGGCGAGGAC
GATCTGCGCAGCGTGGCCGATCTGCTGATCGAACAGATTGAGTTCTGCGACGTGATGCTGGTGAGTAAAACCGACTTGCT
GACCGCGGAACAACAGGGCGAAGTGATGGCGCTGCTCGCCAGCCTGAACCCGGATGCCAAAATCATCGCTATCTCCCCCG
GCAAACTGCCGCTGGAGGCGGTATTGAACACCGGCAGCTTCAGCTTTGACAAAGCGCAGCGGGCTCCCGGCTGGCTTAAG
GAGCTGCGCGGTGAGCACCTGCCTGAAACGGAGAACTATGGCATCAGCAGTTTTGTTTATCGGGCGCGTCGACCTTTTGC
ACCGGATAAATTCTATCGGGTGATCAACGGCGACTGGGGTGGTGGCAAACTGCTGCGTTCCAAAGGGTTCTACTGGTTGG
CGACGCGTCCGCGTCAGGCTGGGCAGTGGAGCCAGGCCGGCGGCATCGCCCATTATGGATTGGCCGGGACTTTCTGGCGC
GCTATCGCGCAGGATAACTGGCCGCAGGACGCAGAGACCCAGGCACAGATTATGGAGAAGTGGGTGGAGCCCTTTGGCGA
TATGCGCCAGGAACTGGTGTTTATCGGGCAGAACCTGCCGCAGGCTGAAATAACGCGTTTGCTGGATGCCTGCCTGCTGA
ACGACGAGGAAATGGCTGCCGGGGTAGACTACTGGCATGAGATGGCCGATCCCTTTCCTGAGTGGTAA

Upstream 100 bases:

>100_bases
TCGCGGCGCGCTGCAATTTTCTGAAGAAGTGGCTGCCACCAACGTTGAACTGGTGCGCGGTTACCAGGCATTACGCAATG
AGTTTTGAGTGAGGAACCCC

Downstream 100 bases:

>100_bases
TTGCACAATGACAGTGTGGTAATGAGAAAAATTCTCAACAAAGTGCTTTACAGGTGATACTGATAACTATTATCATCGCC
TGGCAGTTCACGGAAGGCCA

Product: cobalamin synthesis protein P47K

Products: NA

Alternate protein names: P47K [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MNPLPVTVLSGFLGAGKTTVLNHILNNRQGMRVAVIVNDMSEVNIDAALVENEVQLDRQQEKLVEMSNGCICCTLREDLL
ISVRELAQAGRFDYLLIESSGISEPLPVAETFTFEDEQGESLSHFARLDTLVTVVDGVNFIQQYQQAQTLQEAGQSLGED
DLRSVADLLIEQIEFCDVMLVSKTDLLTAEQQGEVMALLASLNPDAKIIAISPGKLPLEAVLNTGSFSFDKAQRAPGWLK
ELRGEHLPETENYGISSFVYRARRPFAPDKFYRVINGDWGGGKLLRSKGFYWLATRPRQAGQWSQAGGIAHYGLAGTFWR
AIAQDNWPQDAETQAQIMEKWVEPFGDMRQELVFIGQNLPQAEITRLLDACLLNDEEMAAGVDYWHEMADPFPEW

Sequences:

>Translated_395_residues
MNPLPVTVLSGFLGAGKTTVLNHILNNRQGMRVAVIVNDMSEVNIDAALVENEVQLDRQQEKLVEMSNGCICCTLREDLL
ISVRELAQAGRFDYLLIESSGISEPLPVAETFTFEDEQGESLSHFARLDTLVTVVDGVNFIQQYQQAQTLQEAGQSLGED
DLRSVADLLIEQIEFCDVMLVSKTDLLTAEQQGEVMALLASLNPDAKIIAISPGKLPLEAVLNTGSFSFDKAQRAPGWLK
ELRGEHLPETENYGISSFVYRARRPFAPDKFYRVINGDWGGGKLLRSKGFYWLATRPRQAGQWSQAGGIAHYGLAGTFWR
AIAQDNWPQDAETQAQIMEKWVEPFGDMRQELVFIGQNLPQAEITRLLDACLLNDEEMAAGVDYWHEMADPFPEW
>Mature_395_residues
MNPLPVTVLSGFLGAGKTTVLNHILNNRQGMRVAVIVNDMSEVNIDAALVENEVQLDRQQEKLVEMSNGCICCTLREDLL
ISVRELAQAGRFDYLLIESSGISEPLPVAETFTFEDEQGESLSHFARLDTLVTVVDGVNFIQQYQQAQTLQEAGQSLGED
DLRSVADLLIEQIEFCDVMLVSKTDLLTAEQQGEVMALLASLNPDAKIIAISPGKLPLEAVLNTGSFSFDKAQRAPGWLK
ELRGEHLPETENYGISSFVYRARRPFAPDKFYRVINGDWGGGKLLRSKGFYWLATRPRQAGQWSQAGGIAHYGLAGTFWR
AIAQDNWPQDAETQAQIMEKWVEPFGDMRQELVFIGQNLPQAEITRLLDACLLNDEEMAAGVDYWHEMADPFPEW

Specific function: The presence of P47K is critical for the expression of the nitrile hydratase genes. May stabilize or activate the nitrile hydratase proteins [H]

COG id: COG0523

COG function: function code R; Putative GTPases (G3E family)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cobW C-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI33469141, Length=245, Percent_Identity=31.0204081632653, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI126722884, Length=245, Percent_Identity=30.6122448979592, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI146231952, Length=245, Percent_Identity=30.6122448979592, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI148727351, Length=245, Percent_Identity=30.2040816326531, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI223941779, Length=210, Percent_Identity=31.9047619047619, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI223941776, Length=235, Percent_Identity=29.7872340425532, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI119120938, Length=161, Percent_Identity=32.9192546583851, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI87082430, Length=375, Percent_Identity=28, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1788499, Length=109, Percent_Identity=33.9449541284404, Blast_Score=67, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324356, Length=138, Percent_Identity=29.7101449275362, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR011629 [H]

Pfam domain/function: PF02492 cobW; PF07683 CobW_C [H]

EC number: NA

Molecular weight: Translated: 44100; Mature: 44100

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLPVTVLSGFLGAGKTTVLNHILNNRQGMRVAVIVNDMSEVNIDAALVENEVQLDRQQ
CCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEECHHHCCCHHHHHHHHHHHHHHH
EKLVEMSNGCICCTLREDLLISVRELAQAGRFDYLLIESSGISEPLPVAETFTFEDEQGE
HHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEECCCCCCC
SLSHFARLDTLVTVVDGVNFIQQYQQAQTLQEAGQSLGEDDLRSVADLLIEQIEFCDVML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEE
VSKTDLLTAEQQGEVMALLASLNPDAKIIAISPGKLPLEAVLNTGSFSFDKAQRAPGWLK
ECCCHHHCCHHCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHCCCCCCCHHHHCCCHHHH
ELRGEHLPETENYGISSFVYRARRPFAPDKFYRVINGDWGGGKLLRSKGFYWLATRPRQA
HHCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHEECCCCCCCHHHHCCCCEEEECCCCCC
GQWSQAGGIAHYGLAGTFWRAIAQDNWPQDAETQAQIMEKWVEPFGDMRQELVFIGQNLP
CCCHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QAEITRLLDACLLNDEEMAAGVDYWHEMADPFPEW
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNPLPVTVLSGFLGAGKTTVLNHILNNRQGMRVAVIVNDMSEVNIDAALVENEVQLDRQQ
CCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEEEECHHHCCCHHHHHHHHHHHHHHH
EKLVEMSNGCICCTLREDLLISVRELAQAGRFDYLLIESSGISEPLPVAETFTFEDEQGE
HHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEECCCCCCC
SLSHFARLDTLVTVVDGVNFIQQYQQAQTLQEAGQSLGEDDLRSVADLLIEQIEFCDVML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEE
VSKTDLLTAEQQGEVMALLASLNPDAKIIAISPGKLPLEAVLNTGSFSFDKAQRAPGWLK
ECCCHHHCCHHCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHCCCCCCCHHHHCCCHHHH
ELRGEHLPETENYGISSFVYRARRPFAPDKFYRVINGDWGGGKLLRSKGFYWLATRPRQA
HHCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHEECCCCCCCHHHHCCCCEEEECCCCCC
GQWSQAGGIAHYGLAGTFWRAIAQDNWPQDAETQAQIMEKWVEPFGDMRQELVFIGQNLP
CCCHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QAEITRLLDACLLNDEEMAAGVDYWHEMADPFPEW
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2013568 [H]