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The map label for this gene is ycjG [H]

Identifier: 157370855

GI number: 157370855

Start: 2878399

End: 2879379

Strand: Reverse

Name: ycjG [H]

Synonym: Spro_2615

Alternate gene names: 157370855

Gene position: 2879379-2878399 (Counterclockwise)

Preceding gene: 157370857

Following gene: 157370853

Centisome position: 52.84

GC content: 59.73

Gene sequence:

>981_bases
ATGAGAAGCATGCGTTTTTACCCAGAAGCCTGGCCGTTGCATACCGCCTTTGTTATTGCCCGTGGCAGCCGCACCGAAGC
CAAAGTAGTGGTGGTCGAAATTGAGCAGCAGGGCGTGCGGGGGATTGGTGAATGCACTCCTTATCCGCGTTATGGTGAAA
GTGAGGCTTCGGTGATGGAACGGCTGGCCGAAGTGGCTACGGCGGTCGAACGGGGCGTGACGCGCGAGCAACTGCAGCAG
CTAATGCCGGCCGGCGCGGCGCGTAATGCGGTGGATTCCGCCTTGTGGGATTTGGAGTGTCAACTCAGTGGTCAGAGCCG
CTGGCAGCGTAGCGCTGTTGCCGAGCCAGAACGCATTCTGATGGCGCAAACCGTCAGCATAGGTTCGCCGGAAGCCATGG
CCTTCGCCGCACGCGAACTTGAACAGCAAGGGGCACGCTTGCTGAAAATCAAACTGGACAATCATCTGATCAGCGAACGG
CTGGTGGCGATCCGTGCGGCGGTGCCGGATGCCACGTTGATTGTCGATGCCAATGAGTCCTGGCAGGCGGAAGGGCTGGC
GGCGCGCTGCCAACTGTTAGCGGATCTGGGCGTGGCGATGCTGGAGCAACCCCTGCCGGCAACGGATGACAGCGCACTGG
AAAACTTTATCCACCCGCTGCCAATTTGCGCCGATGAAAGCTGCCACACCCGCGGTGATTTACCCAAGCTGGTTGGCCGT
TATCAAATGGTCAATATCAAGCTGGATAAAACCGGCGGCCTGACCGAAGGGCTGGCGCTGGCGCAGGAAGCGCGGCAACA
AGGGTTCGCTATTATGCTCGGTTGCATGTTGTGCACTTCGCGGGCGATCAATGCCGCATTGCCGCTGGCACCGGGCGCGC
GATTCGTCGATTTGGACGGCCCGACCTGGCTGGCTAATGACGTTGAACCGGGGTTGGATTTTGAATGTGGGGCGATTAAT
CTCGCCCCATCGCCGGTTTAG

Upstream 100 bases:

>100_bases
TAGATAAAGTTTGCTAAATAGTTGTATATATTAGAGTGTGTCCAAAAAAGCACCCGCGCGTTCGCCCGATTTTATACGCA
ATTTCCGTTCAGGAGCCGTT

Downstream 100 bases:

>100_bases
CCCGGGTAAGTCAGCAATTCTACCATTGCGTTGAGGTAGTTTTCGCTGGCCGCATCGGCCGAGATCGGCGGGAATTCCGC
AGTAATGCAAGGCAGTGACA

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MRSMRFYPEAWPLHTAFVIARGSRTEAKVVVVEIEQQGVRGIGECTPYPRYGESEASVMERLAEVATAVERGVTREQLQQ
LMPAGAARNAVDSALWDLECQLSGQSRWQRSAVAEPERILMAQTVSIGSPEAMAFAARELEQQGARLLKIKLDNHLISER
LVAIRAAVPDATLIVDANESWQAEGLAARCQLLADLGVAMLEQPLPATDDSALENFIHPLPICADESCHTRGDLPKLVGR
YQMVNIKLDKTGGLTEGLALAQEARQQGFAIMLGCMLCTSRAINAALPLAPGARFVDLDGPTWLANDVEPGLDFECGAIN
LAPSPV

Sequences:

>Translated_326_residues
MRSMRFYPEAWPLHTAFVIARGSRTEAKVVVVEIEQQGVRGIGECTPYPRYGESEASVMERLAEVATAVERGVTREQLQQ
LMPAGAARNAVDSALWDLECQLSGQSRWQRSAVAEPERILMAQTVSIGSPEAMAFAARELEQQGARLLKIKLDNHLISER
LVAIRAAVPDATLIVDANESWQAEGLAARCQLLADLGVAMLEQPLPATDDSALENFIHPLPICADESCHTRGDLPKLVGR
YQMVNIKLDKTGGLTEGLALAQEARQQGFAIMLGCMLCTSRAINAALPLAPGARFVDLDGPTWLANDVEPGLDFECGAIN
LAPSPV
>Mature_326_residues
MRSMRFYPEAWPLHTAFVIARGSRTEAKVVVVEIEQQGVRGIGECTPYPRYGESEASVMERLAEVATAVERGVTREQLQQ
LMPAGAARNAVDSALWDLECQLSGQSRWQRSAVAEPERILMAQTVSIGSPEAMAFAARELEQQGARLLKIKLDNHLISER
LVAIRAAVPDATLIVDANESWQAEGLAARCQLLADLGVAMLEQPLPATDDSALENFIHPLPICADESCHTRGDLPKLVGR
YQMVNIKLDKTGGLTEGLALAQEARQQGFAIMLGCMLCTSRAINAALPLAPGARFVDLDGPTWLANDVEPGLDFECGAIN
LAPSPV

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=321, Percent_Identity=70.7165109034268, Blast_Score=459, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 35311; Mature: 35311

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSMRFYPEAWPLHTAFVIARGSRTEAKVVVVEIEQQGVRGIGECTPYPRYGESEASVME
CCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHH
RLAEVATAVERGVTREQLQQLMPAGAARNAVDSALWDLECQLSGQSRWQRSAVAEPERIL
HHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHCEEEEECCCHHHHHHHCCCHHHHH
MAQTVSIGSPEAMAFAARELEQQGARLLKIKLDNHLISERLVAIRAAVPDATLIVDANES
HHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHCCCCEEEEEECCCC
WQAEGLAARCQLLADLGVAMLEQPLPATDDSALENFIHPLPICADESCHTRGDLPKLVGR
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCC
YQMVNIKLDKTGGLTEGLALAQEARQQGFAIMLGCMLCTSRAINAALPLAPGARFVDLDG
EEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCC
PTWLANDVEPGLDFECGAINLAPSPV
CEEECCCCCCCCCCCCCEEECCCCCC
>Mature Secondary Structure
MRSMRFYPEAWPLHTAFVIARGSRTEAKVVVVEIEQQGVRGIGECTPYPRYGESEASVME
CCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHH
RLAEVATAVERGVTREQLQQLMPAGAARNAVDSALWDLECQLSGQSRWQRSAVAEPERIL
HHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHCEEEEECCCHHHHHHHCCCHHHHH
MAQTVSIGSPEAMAFAARELEQQGARLLKIKLDNHLISERLVAIRAAVPDATLIVDANES
HHHHCCCCCCHHHHHHHHHHHHCCCEEEEEEECHHHHHHHHHHHHHCCCCEEEEEECCCC
WQAEGLAARCQLLADLGVAMLEQPLPATDDSALENFIHPLPICADESCHTRGDLPKLVGR
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCC
YQMVNIKLDKTGGLTEGLALAQEARQQGFAIMLGCMLCTSRAINAALPLAPGARFVDLDG
EEEEEEEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCC
PTWLANDVEPGLDFECGAINLAPSPV
CEEECCCCCCCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]