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The map label for this gene is gcvA [H]

Identifier: 157370788

GI number: 157370788

Start: 2803982

End: 2804968

Strand: Reverse

Name: gcvA [H]

Synonym: Spro_2548

Alternate gene names: 157370788

Gene position: 2804968-2803982 (Counterclockwise)

Preceding gene: 157370798

Following gene: 157370787

Centisome position: 51.48

GC content: 55.22

Gene sequence:

>987_bases
ATGTGTGTTTTTAATTTATTGATTTATTTGTATATTAATTTTAAACCGCCGACGATGTTTAACCGAAATAGTCAACATAT
GAAAAAAACAGAACAATACGTCACAGCGTTGCCTAACGATCCGCCGCTGCGCGCGGTGCGCGCGTTTGAGGCTATCGCCC
GCCTGGGTAGCATCACCGCCGCGGCGAAAGAACTTGCGATATCCCCTTCGGCAGTCAGTCACCAACTCAAAACGCTGGAG
GCCTTTCTGCAAATGCCGCTGACCGAGCGGCAGGGGCGTAACCTGATCCTGCGCAACGAAGGGCGGGATTATTACCGCTC
TATTCGCTCGGCGTTTAACGTATTACGCCAGGCGACCGAGCAGGTGCGTGAGCAATCGGCTTCCCGGCAGGTCACCATCA
GCCTGATCCCGCTGTTCGGCATGGGCTGGTTTATTCCGCGTTTGCATGGGTTTCTGCGCGAAAACCCTGATATCGACATC
AATGTGGTGTACGCCAACCACCGTAATTACCTCAGTGACGCTGCCGATATCTCGATTCGTTTTGGCACCGGGCAATGGAG
CGGTTATCACAGTGAAAAACTGATGTCCGGCAAAATGGTGCCGGTGTGCAGTCGGGGGTTTATCAAGGTGCACGGACTGT
TGGACACGCCGGATCAACTTTCACCACTGCCGCTGCTGCATGACGAAGAACGCACCACCTGGATGCAATGGTTTCAGTTG
GCCGGAGTCCGTCGGCCGCTACGCAGTACCGGCCCGATGTTTGAAGATGGCTTGCTGACGCTGGCGGCGGTGCAGGCGGG
TTTGGGCTGCGCCCTGATGCGTGAGCCGTTGATCGCGCCTTACCTCAGCAGCGGCGAACTGGTTAAGCTGTTTGACCTGG
CAATTGATGACGGCCGAGATTACTACCTGTGCGTAAGGCAGAACACCGAACTGTCGCCGGAAGGGCTGAACCTGCAACGC
TGGTTGCTCAAAGAGGCCGAAGGTTGA

Upstream 100 bases:

>100_bases
ATCCATAGGGGGGAGTCCCGAAAAATTATGATTCAGGAAGCTCAGAGTAGAGAGTTAGCGAAAATTGTAAAATACGGAAA
TATAATTTCTTATACAGAAA

Downstream 100 bases:

>100_bases
ATAAGGCCACGTGCTAACGGCCTGAACTGTCTATCCTGAATATATCGTGATGGCTTATTCAGGAACGCTTGATGACCACA
GAGAATGAACCGGAAACGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MCVFNLLIYLYINFKPPTMFNRNSQHMKKTEQYVTALPNDPPLRAVRAFEAIARLGSITAAAKELAISPSAVSHQLKTLE
AFLQMPLTERQGRNLILRNEGRDYYRSIRSAFNVLRQATEQVREQSASRQVTISLIPLFGMGWFIPRLHGFLRENPDIDI
NVVYANHRNYLSDAADISIRFGTGQWSGYHSEKLMSGKMVPVCSRGFIKVHGLLDTPDQLSPLPLLHDEERTTWMQWFQL
AGVRRPLRSTGPMFEDGLLTLAAVQAGLGCALMREPLIAPYLSSGELVKLFDLAIDDGRDYYLCVRQNTELSPEGLNLQR
WLLKEAEG

Sequences:

>Translated_328_residues
MCVFNLLIYLYINFKPPTMFNRNSQHMKKTEQYVTALPNDPPLRAVRAFEAIARLGSITAAAKELAISPSAVSHQLKTLE
AFLQMPLTERQGRNLILRNEGRDYYRSIRSAFNVLRQATEQVREQSASRQVTISLIPLFGMGWFIPRLHGFLRENPDIDI
NVVYANHRNYLSDAADISIRFGTGQWSGYHSEKLMSGKMVPVCSRGFIKVHGLLDTPDQLSPLPLLHDEERTTWMQWFQL
AGVRRPLRSTGPMFEDGLLTLAAVQAGLGCALMREPLIAPYLSSGELVKLFDLAIDDGRDYYLCVRQNTELSPEGLNLQR
WLLKEAEG
>Mature_328_residues
MCVFNLLIYLYINFKPPTMFNRNSQHMKKTEQYVTALPNDPPLRAVRAFEAIARLGSITAAAKELAISPSAVSHQLKTLE
AFLQMPLTERQGRNLILRNEGRDYYRSIRSAFNVLRQATEQVREQSASRQVTISLIPLFGMGWFIPRLHGFLRENPDIDI
NVVYANHRNYLSDAADISIRFGTGQWSGYHSEKLMSGKMVPVCSRGFIKVHGLLDTPDQLSPLPLLHDEERTTWMQWFQL
AGVRRPLRSTGPMFEDGLLTLAAVQAGLGCALMREPLIAPYLSSGELVKLFDLAIDDGRDYYLCVRQNTELSPEGLNLQR
WLLKEAEG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=288, Percent_Identity=34.7222222222222, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1788706, Length=276, Percent_Identity=31.8840579710145, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1786448, Length=249, Percent_Identity=33.3333333333333, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1789639, Length=182, Percent_Identity=26.3736263736264, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081978, Length=262, Percent_Identity=26.7175572519084, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1786401, Length=253, Percent_Identity=25.296442687747, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 37198; Mature: 37198

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCVFNLLIYLYINFKPPTMFNRNSQHMKKTEQYVTALPNDPPLRAVRAFEAIARLGSITA
CHHHHEEEEHEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AAKELAISPSAVSHQLKTLEAFLQMPLTERQGRNLILRNEGRDYYRSIRSAFNVLRQATE
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
QVREQSASRQVTISLIPLFGMGWFIPRLHGFLRENPDIDINVVYANHRNYLSDAADISIR
HHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHCCCCEEEEE
FGTGQWSGYHSEKLMSGKMVPVCSRGFIKVHGLLDTPDQLSPLPLLHDEERTTWMQWFQL
ECCCCCCCCCHHHHHCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
AGVRRPLRSTGPMFEDGLLTLAAVQAGLGCALMREPLIAPYLSSGELVKLFDLAIDDGRD
HHHHCCHHHCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEEEEEEEECCCC
YYLCVRQNTELSPEGLNLQRWLLKEAEG
EEEEEECCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MCVFNLLIYLYINFKPPTMFNRNSQHMKKTEQYVTALPNDPPLRAVRAFEAIARLGSITA
CHHHHEEEEHEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AAKELAISPSAVSHQLKTLEAFLQMPLTERQGRNLILRNEGRDYYRSIRSAFNVLRQATE
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHH
QVREQSASRQVTISLIPLFGMGWFIPRLHGFLRENPDIDINVVYANHRNYLSDAADISIR
HHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHCCCCEEEEE
FGTGQWSGYHSEKLMSGKMVPVCSRGFIKVHGLLDTPDQLSPLPLLHDEERTTWMQWFQL
ECCCCCCCCCHHHHHCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
AGVRRPLRSTGPMFEDGLLTLAAVQAGLGCALMREPLIAPYLSSGELVKLFDLAIDDGRD
HHHHCCHHHCCCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEEEEEEEECCCC
YYLCVRQNTELSPEGLNLQRWLLKEAEG
EEEEEECCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]