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The map label for this gene is palH [H]

Identifier: 157370763

GI number: 157370763

Start: 2771616

End: 2772977

Strand: Reverse

Name: palH [H]

Synonym: Spro_2523

Alternate gene names: 157370763

Gene position: 2772977-2771616 (Counterclockwise)

Preceding gene: 157370764

Following gene: 157370762

Centisome position: 50.89

GC content: 56.39

Gene sequence:

>1362_bases
ATGGCTACAAAAATCGTTTTAGTGGGTGCGGGCAGCGCGCAATTCGGCTACGGCACGCTGGGGGATATCTTCCAGAGCAA
AACGTTGTACGGCAGCGAAATTGTGCTGCATGACATCAACCCGGCCGCTCTGGCGGTGACCGAGAAAACCGCCCGGGAAT
TTTTGGCGGCGGAAGATTTACCCTTTACCGTCAGTGCCACCACCGATCGTAAAACCGCGCTGCGCGGGGCGGAGTTTGTG
ATTATCTCCATTGAGGTGGGCGACCGCTTTGCGCTGTGGGATCTGGACTGGCAAATCCCGCAGCAATACGGCATTGGGCA
GGTGTATGGCGAAAACGGCGGCCCCGGCGGGCTATTCCACTCGCTGCGGATTATTCCGCCGATCCTCGACATCTGCGCCG
ACGTCGCCGATATCTGCCCAAATGCCTGGATATTTAACTATTCCAACCCGATGAGCCGTATCTGCACCACCGTGCATCGT
CGCTTCCCGCAGCTCAATTTTGTCGGCATGTGCCACGAAATTGCCTCGCTGGAGCGCTATCTGCCGGAAATGCTCGGCAC
TTCGTTTGAAAACCTCAACCTGCGGGCCGCCGGGCTTAACCACTTTAGCGTACTGCTGGAAGCCAGCTACAAAGACAGCG
GTAAAGACGCTTATGCCGATGTGCGTGCCAAGGCACCGGATTATTTTTCCCGCCTGCCGGGCTACAGCGACATTCTGGCT
TACACCCGTACCCACGGCAAACTGGTACAGACCGAGGGCAGCACCGAGCGCGATGCGCTGGGCGGTAAAGACAGTGCCTA
TCCCTGGGCCGACCGCACGCTGTTTAAAGAAATTCTGGAAAAATTCCACCACTTGCCGATCACCGGCGACAGTCATTTTG
GCGAATACATCCGCTGGGCCAGTGAGGTCAGCGATCATCGCGGAATCCTCGATTTCTACACCTTCTATCGCAATTACCTC
GGCCATGTGCAGCCGAAAATAGAACTGAAGCTGAAAGAGCGCGTGGTGCCGATCATGGAAGGGATCCTGACCGATTCCGG
CTATGAAGAGTCGGCGGTCAATATCCCGAACCTGGGGTTTATCAAACAATTGCCGGAATTTATTGCGGTTGAAGTGCCGG
CCATTATCGATCGCAAGGGCGTGCATGGCATCAAGGTCAATATGCCGGCCGGCATCGGTGGCTTGCTCAGCAATCAGATT
GCCATTCACGATCTGACCGCCGACGCGGTCATTGAAGGCTCGCGTGACCTGGTGATCCAGGCGCTGTTGGTCGATTCGGT
CAACGACAAGTGCCGGGCGATCCCCGAACTGGTGGACGTGATGATTTCACGCCAGGGGCCGTGGCTCGATTACCTTAAAT
AA

Upstream 100 bases:

>100_bases
ATCGCACTGGTTCTGATTTTCCAGCGCCGTATCGTCAGCGGATTAACCAACGGCGCAGTAAAAGGCTGATTTTCGATTAA
CCCTAAGCAGGAGCAAACCA

Downstream 100 bases:

>100_bases
GCCGGATGGCGGGGCGCTGGTCGGCGTCCCGCCAAATGCCAATTGGGTGTGAGAACGGGAGGAATATGGCGCAATTAACG
CTGGAAAAACTACAAAAACG

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MATKIVLVGAGSAQFGYGTLGDIFQSKTLYGSEIVLHDINPAALAVTEKTAREFLAAEDLPFTVSATTDRKTALRGAEFV
IISIEVGDRFALWDLDWQIPQQYGIGQVYGENGGPGGLFHSLRIIPPILDICADVADICPNAWIFNYSNPMSRICTTVHR
RFPQLNFVGMCHEIASLERYLPEMLGTSFENLNLRAAGLNHFSVLLEASYKDSGKDAYADVRAKAPDYFSRLPGYSDILA
YTRTHGKLVQTEGSTERDALGGKDSAYPWADRTLFKEILEKFHHLPITGDSHFGEYIRWASEVSDHRGILDFYTFYRNYL
GHVQPKIELKLKERVVPIMEGILTDSGYEESAVNIPNLGFIKQLPEFIAVEVPAIIDRKGVHGIKVNMPAGIGGLLSNQI
AIHDLTADAVIEGSRDLVIQALLVDSVNDKCRAIPELVDVMISRQGPWLDYLK

Sequences:

>Translated_453_residues
MATKIVLVGAGSAQFGYGTLGDIFQSKTLYGSEIVLHDINPAALAVTEKTAREFLAAEDLPFTVSATTDRKTALRGAEFV
IISIEVGDRFALWDLDWQIPQQYGIGQVYGENGGPGGLFHSLRIIPPILDICADVADICPNAWIFNYSNPMSRICTTVHR
RFPQLNFVGMCHEIASLERYLPEMLGTSFENLNLRAAGLNHFSVLLEASYKDSGKDAYADVRAKAPDYFSRLPGYSDILA
YTRTHGKLVQTEGSTERDALGGKDSAYPWADRTLFKEILEKFHHLPITGDSHFGEYIRWASEVSDHRGILDFYTFYRNYL
GHVQPKIELKLKERVVPIMEGILTDSGYEESAVNIPNLGFIKQLPEFIAVEVPAIIDRKGVHGIKVNMPAGIGGLLSNQI
AIHDLTADAVIEGSRDLVIQALLVDSVNDKCRAIPELVDVMISRQGPWLDYLK
>Mature_452_residues
ATKIVLVGAGSAQFGYGTLGDIFQSKTLYGSEIVLHDINPAALAVTEKTAREFLAAEDLPFTVSATTDRKTALRGAEFVI
ISIEVGDRFALWDLDWQIPQQYGIGQVYGENGGPGGLFHSLRIIPPILDICADVADICPNAWIFNYSNPMSRICTTVHRR
FPQLNFVGMCHEIASLERYLPEMLGTSFENLNLRAAGLNHFSVLLEASYKDSGKDAYADVRAKAPDYFSRLPGYSDILAY
TRTHGKLVQTEGSTERDALGGKDSAYPWADRTLFKEILEKFHHLPITGDSHFGEYIRWASEVSDHRGILDFYTFYRNYLG
HVQPKIELKLKERVVPIMEGILTDSGYEESAVNIPNLGFIKQLPEFIAVEVPAIIDRKGVHGIKVNMPAGIGGLLSNQIA
IHDLTADAVIEGSRDLVIQALLVDSVNDKCRAIPELVDVMISRQGPWLDYLK

Specific function: Alpha-glucosidase with broad specificity. Hydrolyzes maltose, palatinose, maltulose, trehalose, trehalulose, turanose, leucrose, sucrose and maltitol. Is not active against alpha- galactosides, e.g. melibiose, and alpha-mannosides. Shows an obligate requi

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790560, Length=472, Percent_Identity=26.6949152542373, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1788029, Length=459, Percent_Identity=24.400871459695, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 50237; Mature: 50106

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKIVLVGAGSAQFGYGTLGDIFQSKTLYGSEIVLHDINPAALAVTEKTAREFLAAEDL
CCEEEEEEECCCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHCCC
PFTVSATTDRKTALRGAEFVIISIEVGDRFALWDLDWQIPQQYGIGQVYGENGGPGGLFH
CEEEECCCCHHHHHCCCEEEEEEEEECCEEEEEECCCCCCCCCCCCEEECCCCCCCHHHH
SLRIIPPILDICADVADICPNAWIFNYSNPMSRICTTVHRRFPQLNFVGMCHEIASLERY
HHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LPEMLGTSFENLNLRAAGLNHFSVLLEASYKDSGKDAYADVRAKAPDYFSRLPGYSDILA
HHHHHCCCCCCCEEEEECCCEEEEEEEECCCCCCCCHHHHHHCCCCHHHHHCCCHHHHHH
YTRTHGKLVQTEGSTERDALGGKDSAYPWADRTLFKEILEKFHHLPITGDSHFGEYIRWA
HHHCCCEEEECCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
SEVSDHRGILDFYTFYRNYLGHVQPKIELKLKERVVPIMEGILTDSGYEESAVNIPNLGF
HHHHHCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
IKQLPEFIAVEVPAIIDRKGVHGIKVNMPAGIGGLLSNQIAIHDLTADAVIEGSRDLVIQ
HHHHHHHHEECCCHHHCCCCCCEEEEECCCCCCHHHCCCEEEEECCHHHHCCCCHHHHHH
ALLVDSVNDKCRAIPELVDVMISRQGPWLDYLK
HHHHHCCCHHHHHHHHHHHHHHCCCCCCHHCCC
>Mature Secondary Structure 
ATKIVLVGAGSAQFGYGTLGDIFQSKTLYGSEIVLHDINPAALAVTEKTAREFLAAEDL
CEEEEEEECCCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCEEEEEHHHHHHHHHHCCC
PFTVSATTDRKTALRGAEFVIISIEVGDRFALWDLDWQIPQQYGIGQVYGENGGPGGLFH
CEEEECCCCHHHHHCCCEEEEEEEEECCEEEEEECCCCCCCCCCCCEEECCCCCCCHHHH
SLRIIPPILDICADVADICPNAWIFNYSNPMSRICTTVHRRFPQLNFVGMCHEIASLERY
HHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LPEMLGTSFENLNLRAAGLNHFSVLLEASYKDSGKDAYADVRAKAPDYFSRLPGYSDILA
HHHHHCCCCCCCEEEEECCCEEEEEEEECCCCCCCCHHHHHHCCCCHHHHHCCCHHHHHH
YTRTHGKLVQTEGSTERDALGGKDSAYPWADRTLFKEILEKFHHLPITGDSHFGEYIRWA
HHHCCCEEEECCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
SEVSDHRGILDFYTFYRNYLGHVQPKIELKLKERVVPIMEGILTDSGYEESAVNIPNLGF
HHHHHCCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
IKQLPEFIAVEVPAIIDRKGVHGIKVNMPAGIGGLLSNQIAIHDLTADAVIEGSRDLVIQ
HHHHHHHHEECCCHHHCCCCCCEEEEECCCCCCHHHCCCEEEEECCHHHHCCCCHHHHHH
ALLVDSVNDKCRAIPELVDVMISRQGPWLDYLK
HHHHHCCCHHHHHHHHHHHHHHCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11274100 [H]