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The map label for this gene is yneF [H]

Identifier: 157370758

GI number: 157370758

Start: 2764041

End: 2765543

Strand: Reverse

Name: yneF [H]

Synonym: Spro_2518

Alternate gene names: 157370758

Gene position: 2765543-2764041 (Counterclockwise)

Preceding gene: 157370759

Following gene: 157370757

Centisome position: 50.75

GC content: 54.69

Gene sequence:

>1503_bases
ATGGCCGCCATCATGCAGGTCGAATTTTATCCGTATTCTTCGCAAGCTTCTGGAAGCAAGATGCACACCACTGAGTTACC
CGACGCCACTAAATTGCGCCTCGCGCTAATCGTCTTTTTGCTGGTGTTCTGCACCTGCATTTTGGGTATTGAAAGTCGTC
CCATCGGTCTGTTAGCGCTATTTTGGCCAACCAATGCGGTACTTTTGGCCGTGCTGGTACGCTACCCGGCCATGGGCAGT
CCTGTTGGCTGGTTGATGGCGATGTCAGGCTATTTGGCGGCGGATCTGATCACCGGCAGCCCTCTGGGCAAAGCCATACT
GCTCAATGGTGCCAATATTGCCGGTGTGGCGGTCGGGTTTTATCTGTTTATGCGTTTATCGCCGCAGGTCAGAACCCTGG
AACGCACCTCATCGAGCATTTATCTGTTCGCTATCTGTCTGGCGAGCGCGACAGCTACGGCGTTACTTGGTGCCGGGGCT
TCGGTGCTGCTGTTTGGCCGCCAGTATCTGCCTTCATTGGCGTTGTGGCTGTCTTCGGAATTTACCAACTACATCACGCT
GATGCCGTTTATTCTGGCTTTTCCGCGGGACTGGAAAGGTTATCTTCGCAGCATTTTCCAGGCCGTGACCCTGCGCAGCC
AGTGGCCCAATACCGTCAGAAAAATCGCGGTGTTACTTCTGCTGCTGTTGGCCTGCTTCGGTAGCATCCTGATTGGTGGC
CCCGGAGCGGTGATTTTCCCGATGCCGATCCTGCTATGGTTGGCGATGTCGTTCTCGCTGTTCAGCACAATGATCGCGGT
ATTGGTTTATGTGTTGTGGTGCCACCTGGCGATCGATCTGGGCCTGATTACCACTAACCTTGATATGAAAATGCTGCAAA
ACACGGTGTCGATGCGACTTGGCGTGGCGTTACTGGCGCTGGGACCTATTGCCGTGGCCAGCATGAATTTTGCTCGCAAT
GCGCTGCTGCAAAGGCTTGAGCACGTGGCGAATCATGATGCTCTGACCCATGTATTGACCCGCAGCGCTTTTATGCAGCG
CGGAACGCGGCTGGTCAGCCAAAAGGGCGAGCGGATCTGCATCATGATGCTGGATATCGACTACTTTAAATCGATCAATG
ACCGCTTTGGGCACGCCGGCGGCGATGCGGCGCTGCTGTCCTTTACCCGCGCCATTACTGGCGATCTGCGCGATAACGAT
TTGTTTGGTCGCATGGGGGGTGAAGAATTTGCGATTGTCGCACCCATTGCGCAACAGCAGGATGCGCTATCGCTGGCCGA
ACGTCTGCGTCACCGAGTTGAAATTGAATCCATCAACATGCCCGCAGGCGCTGCTTTACAGATCACCGTCAGCATCGGTA
TGGTCACCTGCATCGGCGGTTCAGGACAAAATCTGGACAACCTGCTGAAAATCGCCGATTTGGCGGTCTATAAAGCCAAA
AATAGCGGCCGTAACCGGGTCGCCACGCCAGAACTCCTGCCGCACCATTCCACCGAGATCTAA

Upstream 100 bases:

>100_bases
TTATATTTTCAATAAACGGATGCCAGACCCGCGAAAACTGGCAATAATGTCAGTCTGGCTGTTCACCAAACAGGCATTGC
CAGACAGGGTGAGCAGGCTA

Downstream 100 bases:

>100_bases
AGCCCGGCCGCCCTCGTCATGGTTGACCCCGCTAATGGGGTCTAAAATGGAAGAGGGCGCAGGCAGTCCCGCCGGCGCCA
TCCCGACTGTCTAATCGACA

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MAAIMQVEFYPYSSQASGSKMHTTELPDATKLRLALIVFLLVFCTCILGIESRPIGLLALFWPTNAVLLAVLVRYPAMGS
PVGWLMAMSGYLAADLITGSPLGKAILLNGANIAGVAVGFYLFMRLSPQVRTLERTSSSIYLFAICLASATATALLGAGA
SVLLFGRQYLPSLALWLSSEFTNYITLMPFILAFPRDWKGYLRSIFQAVTLRSQWPNTVRKIAVLLLLLLACFGSILIGG
PGAVIFPMPILLWLAMSFSLFSTMIAVLVYVLWCHLAIDLGLITTNLDMKMLQNTVSMRLGVALLALGPIAVASMNFARN
ALLQRLEHVANHDALTHVLTRSAFMQRGTRLVSQKGERICIMMLDIDYFKSINDRFGHAGGDAALLSFTRAITGDLRDND
LFGRMGGEEFAIVAPIAQQQDALSLAERLRHRVEIESINMPAGAALQITVSIGMVTCIGGSGQNLDNLLKIADLAVYKAK
NSGRNRVATPELLPHHSTEI

Sequences:

>Translated_500_residues
MAAIMQVEFYPYSSQASGSKMHTTELPDATKLRLALIVFLLVFCTCILGIESRPIGLLALFWPTNAVLLAVLVRYPAMGS
PVGWLMAMSGYLAADLITGSPLGKAILLNGANIAGVAVGFYLFMRLSPQVRTLERTSSSIYLFAICLASATATALLGAGA
SVLLFGRQYLPSLALWLSSEFTNYITLMPFILAFPRDWKGYLRSIFQAVTLRSQWPNTVRKIAVLLLLLLACFGSILIGG
PGAVIFPMPILLWLAMSFSLFSTMIAVLVYVLWCHLAIDLGLITTNLDMKMLQNTVSMRLGVALLALGPIAVASMNFARN
ALLQRLEHVANHDALTHVLTRSAFMQRGTRLVSQKGERICIMMLDIDYFKSINDRFGHAGGDAALLSFTRAITGDLRDND
LFGRMGGEEFAIVAPIAQQQDALSLAERLRHRVEIESINMPAGAALQITVSIGMVTCIGGSGQNLDNLLKIADLAVYKAK
NSGRNRVATPELLPHHSTEI
>Mature_499_residues
AAIMQVEFYPYSSQASGSKMHTTELPDATKLRLALIVFLLVFCTCILGIESRPIGLLALFWPTNAVLLAVLVRYPAMGSP
VGWLMAMSGYLAADLITGSPLGKAILLNGANIAGVAVGFYLFMRLSPQVRTLERTSSSIYLFAICLASATATALLGAGAS
VLLFGRQYLPSLALWLSSEFTNYITLMPFILAFPRDWKGYLRSIFQAVTLRSQWPNTVRKIAVLLLLLLACFGSILIGGP
GAVIFPMPILLWLAMSFSLFSTMIAVLVYVLWCHLAIDLGLITTNLDMKMLQNTVSMRLGVALLALGPIAVASMNFARNA
LLQRLEHVANHDALTHVLTRSAFMQRGTRLVSQKGERICIMMLDIDYFKSINDRFGHAGGDAALLSFTRAITGDLRDNDL
FGRMGGEEFAIVAPIAQQQDALSLAERLRHRVEIESINMPAGAALQITVSIGMVTCIGGSGQNLDNLLKIADLAVYKAKN
SGRNRVATPELLPHHSTEI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=332, Percent_Identity=32.2289156626506, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1787262, Length=173, Percent_Identity=34.1040462427746, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081881, Length=178, Percent_Identity=37.6404494382022, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI145693134, Length=173, Percent_Identity=34.6820809248555, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1786584, Length=166, Percent_Identity=33.7349397590361, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1788381, Length=178, Percent_Identity=32.0224719101124, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788085, Length=180, Percent_Identity=32.2222222222222, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787816, Length=126, Percent_Identity=34.9206349206349, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI87081977, Length=165, Percent_Identity=30.3030303030303, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787056, Length=157, Percent_Identity=28.6624203821656, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787541, Length=182, Percent_Identity=28.021978021978, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 54384; Mature: 54253

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIMQVEFYPYSSQASGSKMHTTELPDATKLRLALIVFLLVFCTCILGIESRPIGLLAL
CCEEEEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
FWPTNAVLLAVLVRYPAMGSPVGWLMAMSGYLAADLITGSPLGKAILLNGANIAGVAVGF
ECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHH
YLFMRLSPQVRTLERTSSSIYLFAICLASATATALLGAGASVLLFGRQYLPSLALWLSSE
HHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FTNYITLMPFILAFPRDWKGYLRSIFQAVTLRSQWPNTVRKIAVLLLLLLACFGSILIGG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECC
PGAVIFPMPILLWLAMSFSLFSTMIAVLVYVLWCHLAIDLGLITTNLDMKMLQNTVSMRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHH
GVALLALGPIAVASMNFARNALLQRLEHVANHDALTHVLTRSAFMQRGTRLVSQKGERIC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IMMLDIDYFKSINDRFGHAGGDAALLSFTRAITGDLRDNDLFGRMGGEEFAIVAPIAQQQ
EEEECHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCHHHH
DALSLAERLRHRVEIESINMPAGAALQITVSIGMVTCIGGSGQNLDNLLKIADLAVYKAK
HHHHHHHHHHHHCCCEEECCCCCCEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCC
NSGRNRVATPELLPHHSTEI
CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AAIMQVEFYPYSSQASGSKMHTTELPDATKLRLALIVFLLVFCTCILGIESRPIGLLAL
CEEEEEEEECCCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
FWPTNAVLLAVLVRYPAMGSPVGWLMAMSGYLAADLITGSPLGKAILLNGANIAGVAVGF
ECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHH
YLFMRLSPQVRTLERTSSSIYLFAICLASATATALLGAGASVLLFGRQYLPSLALWLSSE
HHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
FTNYITLMPFILAFPRDWKGYLRSIFQAVTLRSQWPNTVRKIAVLLLLLLACFGSILIGG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECC
PGAVIFPMPILLWLAMSFSLFSTMIAVLVYVLWCHLAIDLGLITTNLDMKMLQNTVSMRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHH
GVALLALGPIAVASMNFARNALLQRLEHVANHDALTHVLTRSAFMQRGTRLVSQKGERIC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IMMLDIDYFKSINDRFGHAGGDAALLSFTRAITGDLRDNDLFGRMGGEEFAIVAPIAQQQ
EEEECHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCHHHH
DALSLAERLRHRVEIESINMPAGAALQITVSIGMVTCIGGSGQNLDNLLKIADLAVYKAK
HHHHHHHHHHHHCCCEEECCCCCCEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCC
NSGRNRVATPELLPHHSTEI
CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]