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The map label for this gene is oprM [H]

Identifier: 157370717

GI number: 157370717

Start: 2714519

End: 2715973

Strand: Reverse

Name: oprM [H]

Synonym: Spro_2477

Alternate gene names: 157370717

Gene position: 2715973-2714519 (Counterclockwise)

Preceding gene: 157370718

Following gene: 157370716

Centisome position: 49.84

GC content: 56.98

Gene sequence:

>1455_bases
GTGTTGCAACGTTTAATCACTCTGACATTTCCGCTGTTACTGGCGGGGTGTATCTCGCTGGATCCAAACTACCAGCGCCC
AGCGGCACCGGTTCCCACCACCATGCCACAGGGGGCGGCTTATACTGCGCCTTCCGGTAAATTATCACTGAGCTACCCGG
ATATTCCGTGGCGTGATTATGTGGTCGACGCCAAACTGCGGCAGGTGGTAGAGATGGGGCTGAGCAGCAGTCGAGATCTG
CGTGAAGCCATTGCCAATATTGAATCGGCGCGGGCGCTCTACGGCGAGCAGCGCTCCGATTTGTTCCCGACCATTAACGC
CGGTGTGGACGGCAGCCGTGGGCGGGCACTAACCGGTAACGGTAACGCCACCGCGATCAGCCAGAGCTACGAAGCCACGG
CCGGCACCAGTGCATTTGAGCTGGATTTGTTCGGCAAGAACCGTAGCCTGTCACGTTCGACCTTTGAAACCTATCTGGCA
GAGTCGGAGGCGGCCAAAAGTACTCGCCTGACGTTGATCGCCGATATCGTCACCGACTGGGTGAACGTGGCCGCCGAACG
CAGCAACCTGAATGCGGCCAGGGGCACCATGGAAAGCGCCAAATTGTCACTGGCCGTGACGCGCAAAAACCAGCAAAACG
GCATTCTGTCGATGGTTGATGTCGCTACGGCGGACACGGTGTATCAGCAGGCTCGCTCGGACGTGGCCAGCTACACCACC
AGCGCCGCACAGGCGAAAAATGCGCTGGATCTGGTGGTGGGGCAAAGTGTACCTGAGAACCTGTTGCCGACGGATATCGC
AGCATTGTCCGGCATAATGAAAGATATTCCGGCGAATCTGTCATCCCAGGTGCTGCTGAACCGTCCGGACGTGCTGGAGG
CTGAGCATAACCTGAAGGCGGCCAACGCCAATATCGGTTCCGCTCGCGCAGCGTTTTTCCCGTCTATCAATCTGACGGCC
AGTGGCGGTATAGGCAGTGGGGAATTGTCATCACTGTTCAGCAAGGGGGCTGGCGTCTGGTCATTTTCACCGAGTATCAG
CCTGCCGATTTTCACCGGCGGCTATAACACCGCCCAGTTGAATTACACCAGGGCGCAGAAAGACCTGTACGTTGCCACCT
ACGAGAAAGCGGTGCAGACGGCATTCCAGGAAACTGCCGATGCCCTGGCGCGTAAGGGCACTATTCAGGAACAGTTAGCG
GCACAAACCGGCTATGTGGCGGCTTCACAGGAATATTATCGCCTGGCAGAGCTGCGTTATCGCCACGGGGTGGATACCTA
TCTGACGATGCTTGACGCGCAGCGTACCTTGTATAGCGCTCGTCAGTCGTTGATTACCACTCAGCAAACCGAATACCAAA
ACCTGATCACGCTGTATAAAGTGCTGGGCGGGGGTATTAGCGCAGAGAAGCAGGGCGATGGCGTTATTGCCAATAATGTC
TCCCTGACCCAATAA

Upstream 100 bases:

>100_bases
TTCGTTCCCTTGTTCTACGTCTTGGTGCGCCGGATGTTTTCACGCAAAACCACCGTCGCCAAACCGCTGGAAAACACTGC
GCCGCAGGCAGGAGAATAAT

Downstream 100 bases:

>100_bases
GATTTACTGTAAACAACCATGCTAATACGCAGGATGCCGACAACATTCTGCGTATTTTTATATTCACTCTGTGGTGAAAG
TGTAAAGAAAAGGAAAATCA

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MLQRLITLTFPLLLAGCISLDPNYQRPAAPVPTTMPQGAAYTAPSGKLSLSYPDIPWRDYVVDAKLRQVVEMGLSSSRDL
REAIANIESARALYGEQRSDLFPTINAGVDGSRGRALTGNGNATAISQSYEATAGTSAFELDLFGKNRSLSRSTFETYLA
ESEAAKSTRLTLIADIVTDWVNVAAERSNLNAARGTMESAKLSLAVTRKNQQNGILSMVDVATADTVYQQARSDVASYTT
SAAQAKNALDLVVGQSVPENLLPTDIAALSGIMKDIPANLSSQVLLNRPDVLEAEHNLKAANANIGSARAAFFPSINLTA
SGGIGSGELSSLFSKGAGVWSFSPSISLPIFTGGYNTAQLNYTRAQKDLYVATYEKAVQTAFQETADALARKGTIQEQLA
AQTGYVAASQEYYRLAELRYRHGVDTYLTMLDAQRTLYSARQSLITTQQTEYQNLITLYKVLGGGISAEKQGDGVIANNV
SLTQ

Sequences:

>Translated_484_residues
MLQRLITLTFPLLLAGCISLDPNYQRPAAPVPTTMPQGAAYTAPSGKLSLSYPDIPWRDYVVDAKLRQVVEMGLSSSRDL
REAIANIESARALYGEQRSDLFPTINAGVDGSRGRALTGNGNATAISQSYEATAGTSAFELDLFGKNRSLSRSTFETYLA
ESEAAKSTRLTLIADIVTDWVNVAAERSNLNAARGTMESAKLSLAVTRKNQQNGILSMVDVATADTVYQQARSDVASYTT
SAAQAKNALDLVVGQSVPENLLPTDIAALSGIMKDIPANLSSQVLLNRPDVLEAEHNLKAANANIGSARAAFFPSINLTA
SGGIGSGELSSLFSKGAGVWSFSPSISLPIFTGGYNTAQLNYTRAQKDLYVATYEKAVQTAFQETADALARKGTIQEQLA
AQTGYVAASQEYYRLAELRYRHGVDTYLTMLDAQRTLYSARQSLITTQQTEYQNLITLYKVLGGGISAEKQGDGVIANNV
SLTQ
>Mature_484_residues
MLQRLITLTFPLLLAGCISLDPNYQRPAAPVPTTMPQGAAYTAPSGKLSLSYPDIPWRDYVVDAKLRQVVEMGLSSSRDL
REAIANIESARALYGEQRSDLFPTINAGVDGSRGRALTGNGNATAISQSYEATAGTSAFELDLFGKNRSLSRSTFETYLA
ESEAAKSTRLTLIADIVTDWVNVAAERSNLNAARGTMESAKLSLAVTRKNQQNGILSMVDVATADTVYQQARSDVASYTT
SAAQAKNALDLVVGQSVPENLLPTDIAALSGIMKDIPANLSSQVLLNRPDVLEAEHNLKAANANIGSARAAFFPSINLTA
SGGIGSGELSSLFSKGAGVWSFSPSISLPIFTGGYNTAQLNYTRAQKDLYVATYEKAVQTAFQETADALARKGTIQEQLA
AQTGYVAASQEYYRLAELRYRHGVDTYLTMLDAQRTLYSARQSLITTQQTEYQNLITLYKVLGGGISAEKQGDGVIANNV
SLTQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=466, Percent_Identity=37.1244635193133, Blast_Score=294, Evalue=1e-80,
Organism=Escherichia coli, GI2367348, Length=478, Percent_Identity=24.8953974895398, Blast_Score=104, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51893; Mature: 51893

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQRLITLTFPLLLAGCISLDPNYQRPAAPVPTTMPQGAAYTAPSGKLSLSYPDIPWRDY
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEECCCCCCCHHH
VVDAKLRQVVEMGLSSSRDLREAIANIESARALYGEQRSDLFPTINAGVDGSRGRALTGN
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCEEEECC
GNATAISQSYEATAGTSAFELDLFGKNRSLSRSTFETYLAESEAAKSTRLTLIADIVTDW
CCCEEEECCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNVAAERSNLNAARGTMESAKLSLAVTRKNQQNGILSMVDVATADTVYQQARSDVASYTT
HHHHHHHCCCCHHHCCHHHHEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
SAAQAKNALDLVVGQSVPENLLPTDIAALSGIMKDIPANLSSQVLLNRPDVLEAEHNLKA
HHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEE
ANANIGSARAAFFPSINLTASGGIGSGELSSLFSKGAGVWSFSPSISLPIFTGGYNTAQL
CCCCCCCCHHHCCCCCEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCEEEECCCCEEEE
NYTRAQKDLYVATYEKAVQTAFQETADALARKGTIQEQLAAQTGYVAASQEYYRLAELRY
EEEHHCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEECCHHHHHHHHHHH
RHGVDTYLTMLDAQRTLYSARQSLITTQQTEYQNLITLYKVLGGGISAEKQGDGVIANNV
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCC
SLTQ
CCCC
>Mature Secondary Structure
MLQRLITLTFPLLLAGCISLDPNYQRPAAPVPTTMPQGAAYTAPSGKLSLSYPDIPWRDY
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCEEECCCCCCCHHH
VVDAKLRQVVEMGLSSSRDLREAIANIESARALYGEQRSDLFPTINAGVDGSRGRALTGN
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCEEEECC
GNATAISQSYEATAGTSAFELDLFGKNRSLSRSTFETYLAESEAAKSTRLTLIADIVTDW
CCCEEEECCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNVAAERSNLNAARGTMESAKLSLAVTRKNQQNGILSMVDVATADTVYQQARSDVASYTT
HHHHHHHCCCCHHHCCHHHHEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHH
SAAQAKNALDLVVGQSVPENLLPTDIAALSGIMKDIPANLSSQVLLNRPDVLEAEHNLKA
HHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEE
ANANIGSARAAFFPSINLTASGGIGSGELSSLFSKGAGVWSFSPSISLPIFTGGYNTAQL
CCCCCCCCHHHCCCCCEEEECCCCCCHHHHHHHHCCCCEEEECCCCCCEEEECCCCEEEE
NYTRAQKDLYVATYEKAVQTAFQETADALARKGTIQEQLAAQTGYVAASQEYYRLAELRY
EEEHHCCCEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEECCHHHHHHHHHHH
RHGVDTYLTMLDAQRTLYSARQSLITTQQTEYQNLITLYKVLGGGISAEKQGDGVIANNV
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCC
SLTQ
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]