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The map label for this gene is gcvA [H]

Identifier: 157370706

GI number: 157370706

Start: 2701535

End: 2702452

Strand: Reverse

Name: gcvA [H]

Synonym: Spro_2466

Alternate gene names: 157370706

Gene position: 2702452-2701535 (Counterclockwise)

Preceding gene: 157370711

Following gene: 157370705

Centisome position: 49.6

GC content: 54.68

Gene sequence:

>918_bases
GTGAGAAAACTTCCATCACTCAGTATGTTGCGTGTATTTGAAGAAACCTGCCGTACGCTGAGTTTCAGCAAGGCGGCAGA
GGCGTTGTTTATTACTCAAAGCGCGGCCAGCCGTCAGATAAAACAGCTTGAGGAGTTTCTGGGTAAATTGCTGTTTATCC
GTAGCCACAGCGGCCTGGCGCTAACCCAGGATGCAATTTTGTTGTTGCCGCGGGTACGGCAGTCACTGGATATCCTCGAA
GAGGGGATCCAACAGGTACAGCTGCATAATCCGTTGCAGCATCTGAGGTTACAGGTAGCGCCGACCTTCGCGACCCGCTG
GCTGGCACCGCGACTGGTGGCGTTACGTCGCCGCAACAGCCAGTTGCAGATCGCCCTGATCAGTGAAACCCGGCAAAAAT
ATTGCGATTTTGACTGTGCCATTCGTTTTGGCGAACCGGCCGATTGTGCTGTCAGTGGGGAATGGTTATGCCATGAACGA
CTGGTGCTGGTCGGTAGCCCTTTATTGATGGTTAACGAGGCCTTCCCGCCGTTGCATACCCAGCCGCAGTTGCACATTCT
TAACGGTGATATCCGGCTGGATAACTGGGCGTGCTGGCTGGCGGCGGCGGGGCAAGTGATTGCCGGTCAGCAGAGTGGGT
TGGAGTTCAGTACTCAGGATCAGGTGATTAACGCCTGCATTACCGGTGCCGGCTATGCCGTGCTGGATCGATTGATGATC
CGTAACGAACTGCAAAGCGGGCTGTTGGTGTTACTGTCGCCACTGGAGCTGAAAAGTCGCAACGGCTACTGGTTGGAAAT
TCCGGCCGGCAAGCGCGATTTACCGCGGGTAGGTTACTTCCATGACTGGCTACAGGAGGAAATTGTGCAGCTATCACAGC
TTCCTGAACCGATGTCGGGCATGATGGCGGTGGTTTGA

Upstream 100 bases:

>100_bases
TCTGGTTTTACAGAAATAAGCGCCACAGAGGCTGGCGAATAACTGCAAATTAGCAACATATGGTTAACAGGTTGGTTGCT
GAACAGAGGCGCGGTGAATC

Downstream 100 bases:

>100_bases
GCAGACGTGGCGATACCGGTGTGTGGGTGTGTTACCTTATTGTTAATGAACATTGCCCGTTCCCCATCCGGACGGGCCTG
ACTGGGGAAAGACGTGTCTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MRKLPSLSMLRVFEETCRTLSFSKAAEALFITQSAASRQIKQLEEFLGKLLFIRSHSGLALTQDAILLLPRVRQSLDILE
EGIQQVQLHNPLQHLRLQVAPTFATRWLAPRLVALRRRNSQLQIALISETRQKYCDFDCAIRFGEPADCAVSGEWLCHER
LVLVGSPLLMVNEAFPPLHTQPQLHILNGDIRLDNWACWLAAAGQVIAGQQSGLEFSTQDQVINACITGAGYAVLDRLMI
RNELQSGLLVLLSPLELKSRNGYWLEIPAGKRDLPRVGYFHDWLQEEIVQLSQLPEPMSGMMAVV

Sequences:

>Translated_305_residues
MRKLPSLSMLRVFEETCRTLSFSKAAEALFITQSAASRQIKQLEEFLGKLLFIRSHSGLALTQDAILLLPRVRQSLDILE
EGIQQVQLHNPLQHLRLQVAPTFATRWLAPRLVALRRRNSQLQIALISETRQKYCDFDCAIRFGEPADCAVSGEWLCHER
LVLVGSPLLMVNEAFPPLHTQPQLHILNGDIRLDNWACWLAAAGQVIAGQQSGLEFSTQDQVINACITGAGYAVLDRLMI
RNELQSGLLVLLSPLELKSRNGYWLEIPAGKRDLPRVGYFHDWLQEEIVQLSQLPEPMSGMMAVV
>Mature_305_residues
MRKLPSLSMLRVFEETCRTLSFSKAAEALFITQSAASRQIKQLEEFLGKLLFIRSHSGLALTQDAILLLPRVRQSLDILE
EGIQQVQLHNPLQHLRLQVAPTFATRWLAPRLVALRRRNSQLQIALISETRQKYCDFDCAIRFGEPADCAVSGEWLCHER
LVLVGSPLLMVNEAFPPLHTQPQLHILNGDIRLDNWACWLAAAGQVIAGQQSGLEFSTQDQVINACITGAGYAVLDRLMI
RNELQSGLLVLLSPLELKSRNGYWLEIPAGKRDLPRVGYFHDWLQEEIVQLSQLPEPMSGMMAVV

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=288, Percent_Identity=32.6388888888889, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI1786448, Length=287, Percent_Identity=30.3135888501742, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788706, Length=300, Percent_Identity=27.6666666666667, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=26.4604810996564, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=24.3150684931507, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34342; Mature: 34342

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLPSLSMLRVFEETCRTLSFSKAAEALFITQSAASRQIKQLEEFLGKLLFIRSHSGLA
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHEEEHHHHHHHHHHHHHHHHHHEEEECCCCCE
LTQDAILLLPRVRQSLDILEEGIQQVQLHNPLQHLRLQVAPTFATRWLAPRLVALRRRNS
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHEEEECHHHHHHHHHHHHHHHHHCCC
QLQIALISETRQKYCDFDCAIRFGEPADCAVSGEWLCHERLVLVGSPLLMVNEAFPPLHT
CEEEEEHHHHHHHHCCCCEEEECCCCCCCEECCCHHHHHHHHHHCCCEEEEECCCCCCCC
QPQLHILNGDIRLDNWACWLAAAGQVIAGQQSGLEFSTQDQVINACITGAGYAVLDRLMI
CCCEEEEECCEEECCHHEEEHHCCCEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
RNELQSGLLVLLSPLELKSRNGYWLEIPAGKRDLPRVGYFHDWLQEEIVQLSQLPEPMSG
HHHHHCCCEEEECCHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
MMAVV
HEECC
>Mature Secondary Structure
MRKLPSLSMLRVFEETCRTLSFSKAAEALFITQSAASRQIKQLEEFLGKLLFIRSHSGLA
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHEEEHHHHHHHHHHHHHHHHHHEEEECCCCCE
LTQDAILLLPRVRQSLDILEEGIQQVQLHNPLQHLRLQVAPTFATRWLAPRLVALRRRNS
ECHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHEEEECHHHHHHHHHHHHHHHHHCCC
QLQIALISETRQKYCDFDCAIRFGEPADCAVSGEWLCHERLVLVGSPLLMVNEAFPPLHT
CEEEEEHHHHHHHHCCCCEEEECCCCCCCEECCCHHHHHHHHHHCCCEEEEECCCCCCCC
QPQLHILNGDIRLDNWACWLAAAGQVIAGQQSGLEFSTQDQVINACITGAGYAVLDRLMI
CCCEEEEECCEEECCHHEEEHHCCCEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHH
RNELQSGLLVLLSPLELKSRNGYWLEIPAGKRDLPRVGYFHDWLQEEIVQLSQLPEPMSG
HHHHHCCCEEEECCHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
MMAVV
HEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]