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The map label for this gene is hycB [H]

Identifier: 157370673

GI number: 157370673

Start: 2667322

End: 2667936

Strand: Reverse

Name: hycB [H]

Synonym: Spro_2433

Alternate gene names: 157370673

Gene position: 2667936-2667322 (Counterclockwise)

Preceding gene: 157370674

Following gene: 157370672

Centisome position: 48.96

GC content: 60.0

Gene sequence:

>615_bases
ATGAACCGCTTTATTATTGCGGACCCTAAATTGTGTATTGGCTGCAATACCTGTATGGCCAGTTGTTCTGCGGAACATCG
GCAATATGGATTGCAGTCATTACCCCGCCTGCAGGTGATTCGCAACGCTCGCGACTCTGCGCCGGCGATGTGCCACCAGT
GTGAAGATGCCCCCTGCGCGCAGGTATGTCCGGTCAACGCTATTCGCCATCAGGATGACGCCATCGTACTGAACGAAAGC
CTGTGCATCAGTTGCAAGCTGTGCGGCATTGCCTGCCCGTTTGGTGCCATTGGTTTCGGCGGCAGCACCCCACTGGCGAT
CCCTGCGGACTGCAACACCTCGCTGGCATTGCCTGCGCCCAAAGCACCACGCCCAATCAGCCCCTTCCTGGATTGTGTGC
CCGGTGTCCGTGCCGTGGCGGTGAAGTGCGATCTCTGTGACTTCAGCCCCGAAGGCCCCGCCTGTATCCGCACCTGTCCA
ACCCAGGCGATCCGGCTGGTGACCGACCGAGACGTGCGCAACGCCAGCGAACAGAAAAGGCGCAATGCGATGCAGGCGCT
CAGCGCCGAGTTGCCGTTTGCGGCCGTCCCTGGATCCCACAAGGAGCCAAATTGA

Upstream 100 bases:

>100_bases
CTTACCCGTCTGATCGCAGGCGTTAATCACCGACAGAAAACTGTCGAAGCGTCATTTTTTACTGACGATTTCGTCATAAA
TGACGAAGCAAGGAGTGTGC

Downstream 100 bases:

>100_bases
TGATATATGCCCTGTTGCCCGACCCGTTGTTACTGCTGACTTCGGCCCTGGTCGGGTATTTGCTCTGCGCCCTGTTGGGC
TGGCTACTGGCCCCCTGGTC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: Oxidized ferredoxin; CO2; H2 [C]

Alternate protein names: FHL subunit 2; Hydrogenase-3 component B [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MNRFIIADPKLCIGCNTCMASCSAEHRQYGLQSLPRLQVIRNARDSAPAMCHQCEDAPCAQVCPVNAIRHQDDAIVLNES
LCISCKLCGIACPFGAIGFGGSTPLAIPADCNTSLALPAPKAPRPISPFLDCVPGVRAVAVKCDLCDFSPEGPACIRTCP
TQAIRLVTDRDVRNASEQKRRNAMQALSAELPFAAVPGSHKEPN

Sequences:

>Translated_204_residues
MNRFIIADPKLCIGCNTCMASCSAEHRQYGLQSLPRLQVIRNARDSAPAMCHQCEDAPCAQVCPVNAIRHQDDAIVLNES
LCISCKLCGIACPFGAIGFGGSTPLAIPADCNTSLALPAPKAPRPISPFLDCVPGVRAVAVKCDLCDFSPEGPACIRTCP
TQAIRLVTDRDVRNASEQKRRNAMQALSAELPFAAVPGSHKEPN
>Mature_204_residues
MNRFIIADPKLCIGCNTCMASCSAEHRQYGLQSLPRLQVIRNARDSAPAMCHQCEDAPCAQVCPVNAIRHQDDAIVLNES
LCISCKLCGIACPFGAIGFGGSTPLAIPADCNTSLALPAPKAPRPISPFLDCVPGVRAVAVKCDLCDFSPEGPACIRTCP
TQAIRLVTDRDVRNASEQKRRNAMQALSAELPFAAVPGSHKEPN

Specific function: Probable electron transfer protein for hydrogenase 3 [H]

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789079, Length=181, Percent_Identity=64.6408839779006, Blast_Score=235, Evalue=1e-63,
Organism=Escherichia coli, GI87082114, Length=187, Percent_Identity=52.9411764705882, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI87082179, Length=183, Percent_Identity=38.7978142076503, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1788811, Length=191, Percent_Identity=37.696335078534, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1789067, Length=188, Percent_Identity=37.2340425531915, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI2367245, Length=189, Percent_Identity=34.9206349206349, Blast_Score=110, Evalue=5e-26,
Organism=Escherichia coli, GI87082180, Length=188, Percent_Identity=33.5106382978723, Blast_Score=110, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 21729; Mature: 21729

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
10.8 %Cys+Met (Translated Protein)
8.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
10.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFIIADPKLCIGCNTCMASCSAEHRQYGLQSLPRLQVIRNARDSAPAMCHQCEDAPCA
CCCEEEECCCEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHCCCCCCC
QVCPVNAIRHQDDAIVLNESLCISCKLCGIACPFGAIGFGGSTPLAIPADCNTSLALPAP
HHCCCHHHHCCCCEEEECCCEEEEEEECCEECCCCCCCCCCCCCEEEECCCCCCEECCCC
KAPRPISPFLDCVPGVRAVAVKCDLCDFSPEGPACIRTCPTQAIRLVTDRDVRNASEQKR
CCCCCCCHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHCCHHHHHHHHCCCHHHHHHHHH
RNAMQALSAELPFAAVPGSHKEPN
HHHHHHHHHCCCEEECCCCCCCCC
>Mature Secondary Structure
MNRFIIADPKLCIGCNTCMASCSAEHRQYGLQSLPRLQVIRNARDSAPAMCHQCEDAPCA
CCCEEEECCCEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHCCCCCCC
QVCPVNAIRHQDDAIVLNESLCISCKLCGIACPFGAIGFGGSTPLAIPADCNTSLALPAP
HHCCCHHHHCCCCEEEECCCEEEEEEECCEECCCCCCCCCCCCCEEEECCCCCCEECCCC
KAPRPISPFLDCVPGVRAVAVKCDLCDFSPEGPACIRTCPTQAIRLVTDRDVRNASEQKR
CCCCCCCHHHHHCCCCEEEEEEEECCCCCCCCCHHHHHCCHHHHHHHHCCCHHHHHHHHH
RNAMQALSAELPFAAVPGSHKEPN
HHHHHHHHHCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced ferredoxin; H+; formate [C]

Specific reaction: 2 reduced ferredoxin + 2 H+ = 2 oxidized ferredoxin + H2 formate = CO2 + H2 [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]