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The map label for this gene is pykF [H]

Identifier: 157370427

GI number: 157370427

Start: 2388069

End: 2389481

Strand: Reverse

Name: pykF [H]

Synonym: Spro_2187

Alternate gene names: 157370427

Gene position: 2389481-2388069 (Counterclockwise)

Preceding gene: 157370428

Following gene: 157370426

Centisome position: 43.85

GC content: 54.28

Gene sequence:

>1413_bases
ATGAAAAAGACCAAAATTGTTTGTACCATCGGTCCAAAAACCGAATCGGAAGAAATGCTGACCAACCTGCTGAATGCAGG
CATGAACGTAATGCGCCTTAACTTCTCCCACGGCGATTATGAAGAGCACGGCAACCGCATCAAGAACATGCGCGCCGTGA
TGGCGAAAACCGGTCAGAACGCCGGCATCCTGTTGGATACCAAAGGCCCGGAAATTCGCACCATGAAACTGGAAGGCGGT
AAAGACGCGGCGCTGGTTGCCGGTCAGACCTTCACTTTCACCACCGATCAGAGCGTTATCGGCAACAACGAACGCGTTGC
GGTAACCTACGCCGGCTTTAGCGCTGACCTGAAAATCGGCAACACCGTATTGGTGGATGACGGCTTGATCGGCATGGAAG
TCACCAACGTGACCGAAAGCGAAGTGATCTGTAAGGTGCTGAACAGCGGCGACCTGGGCGAAAACAAAGGCGTTAACCTG
CCGGGCGTTTCCATCCAACTGCCTGCACTGGCCGAAAAAGACAAACGCGACCTGATTTTCGGCTGCGAACAGGGCGTGGA
CTTCGTTGCCGCTTCCTTCATCCGCAAGCGTTCTGACGTGCTGGAAATTCGTGAGCACCTGAAAGCGCACGGCGGCTCGC
AGATCCAAATTATCTCCAAAATCGAAAACCAGGAAGGCCTGAACAACTTCGACGAAATCCTCGAAGCTTCTGACGGCATC
ATGGTTGCTCGTGGCGATCTGGGCGTTGAAATCCCGGTAGAAGAAGTTATCTTCGCTCAGAAGATGATGATCGAAAAATG
TAACCGTGCACGCAAAGTGGTGATCACCGCCACCCAAATGCTCGATTCCATGATCAAGAACCCACGCCCTACCCGTGCAG
AAGCCGGCGACGTAGCTAACGCCATTTTGGACGGTACCGACGCCGTGATGCTGTCTGGTGAGAGCGCCAAGGGCAAGTAC
CCACTGGAAGCCGTTACCATCATGGCGACCATCTGTGAGCGCACCGACCGCGTGATGCCAAGCCGTATCGACAGCCTGCA
TGACAACCGCAAGCTGCGCATCACCGAAGCCGTATGCCGTGGTGCTGTTGAAACCGCAGAGAAACTGGATGCACCACTGA
TCGTAGTGGCCACCAGCGGCGGCAAGTCAGCCAAATCCGTGCGTAAATACTTCCCTAACGCTGTGATCCTGGCGCTGACC
ACCAACGAAACTACCGCTCATCAGCTTATTCTGACCAAGGGCGTGATCCCACAGATGGTCAAGGAAATTGCCTCTACCGA
CGACTTCTATCGCATCGGTAAAGAAGCGGCATTGGCCAGCGGTTTGGCTCAAAAAGGCGACGTTGTAGTGATGGTTTCTG
GTGCACTGGTACCAAGCGGCACTACCAATACTGCCTCGGTGCACGTACTCTAA

Upstream 100 bases:

>100_bases
GTGTTTTGCACCACCAGGCACGACTCAAATCCCTTATCAGTGGCCTGACCGCTGGTTTTATTCGTTTTATTATCCATCAC
AACTTGTAGAAAACTCCGTC

Downstream 100 bases:

>100_bases
TATAAAACGTGACATAATTATTTTGACAAAAACGCCGCTTTGCGGCGTTTTTTTTAACACTGGTAAACCGTTTTCTCAAA
AACGCAACCGTGGATTGCTC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK-1 [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKKTKIVCTIGPKTESEEMLTNLLNAGMNVMRLNFSHGDYEEHGNRIKNMRAVMAKTGQNAGILLDTKGPEIRTMKLEGG
KDAALVAGQTFTFTTDQSVIGNNERVAVTYAGFSADLKIGNTVLVDDGLIGMEVTNVTESEVICKVLNSGDLGENKGVNL
PGVSIQLPALAEKDKRDLIFGCEQGVDFVAASFIRKRSDVLEIREHLKAHGGSQIQIISKIENQEGLNNFDEILEASDGI
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PLEAVTIMATICERTDRVMPSRIDSLHDNRKLRITEAVCRGAVETAEKLDAPLIVVATSGGKSAKSVRKYFPNAVILALT
TNETTAHQLILTKGVIPQMVKEIASTDDFYRIGKEAALASGLAQKGDVVVMVSGALVPSGTTNTASVHVL

Sequences:

>Translated_470_residues
MKKTKIVCTIGPKTESEEMLTNLLNAGMNVMRLNFSHGDYEEHGNRIKNMRAVMAKTGQNAGILLDTKGPEIRTMKLEGG
KDAALVAGQTFTFTTDQSVIGNNERVAVTYAGFSADLKIGNTVLVDDGLIGMEVTNVTESEVICKVLNSGDLGENKGVNL
PGVSIQLPALAEKDKRDLIFGCEQGVDFVAASFIRKRSDVLEIREHLKAHGGSQIQIISKIENQEGLNNFDEILEASDGI
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PLEAVTIMATICERTDRVMPSRIDSLHDNRKLRITEAVCRGAVETAEKLDAPLIVVATSGGKSAKSVRKYFPNAVILALT
TNETTAHQLILTKGVIPQMVKEIASTDDFYRIGKEAALASGLAQKGDVVVMVSGALVPSGTTNTASVHVL
>Mature_470_residues
MKKTKIVCTIGPKTESEEMLTNLLNAGMNVMRLNFSHGDYEEHGNRIKNMRAVMAKTGQNAGILLDTKGPEIRTMKLEGG
KDAALVAGQTFTFTTDQSVIGNNERVAVTYAGFSADLKIGNTVLVDDGLIGMEVTNVTESEVICKVLNSGDLGENKGVNL
PGVSIQLPALAEKDKRDLIFGCEQGVDFVAASFIRKRSDVLEIREHLKAHGGSQIQIISKIENQEGLNNFDEILEASDGI
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKY
PLEAVTIMATICERTDRVMPSRIDSLHDNRKLRITEAVCRGAVETAEKLDAPLIVVATSGGKSAKSVRKYFPNAVILALT
TNETTAHQLILTKGVIPQMVKEIASTDDFYRIGKEAALASGLAQKGDVVVMVSGALVPSGTTNTASVHVL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=486, Percent_Identity=46.7078189300411, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI33286420, Length=486, Percent_Identity=46.7078189300411, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI33286418, Length=487, Percent_Identity=46.8172484599589, Blast_Score=394, Evalue=1e-110,
Organism=Homo sapiens, GI10835121, Length=486, Percent_Identity=46.0905349794239, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI32967597, Length=486, Percent_Identity=46.0905349794239, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=49.3055555555556, Blast_Score=255, Evalue=7e-68,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=49.3055555555556, Blast_Score=255, Evalue=7e-68,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=43.6440677966102, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=43.6440677966102, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=43.2558139534884, Blast_Score=161, Evalue=1e-39,
Organism=Escherichia coli, GI1787965, Length=470, Percent_Identity=87.0212765957447, Blast_Score=842, Evalue=0.0,
Organism=Escherichia coli, GI1788160, Length=482, Percent_Identity=37.551867219917, Blast_Score=274, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI17544584, Length=480, Percent_Identity=41.875, Blast_Score=360, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI17506831, Length=483, Percent_Identity=45.9627329192547, Blast_Score=352, Evalue=3e-97,
Organism=Caenorhabditis elegans, GI71984406, Length=483, Percent_Identity=45.9627329192547, Blast_Score=352, Evalue=3e-97,
Organism=Caenorhabditis elegans, GI71984413, Length=480, Percent_Identity=46.25, Blast_Score=352, Evalue=3e-97,
Organism=Caenorhabditis elegans, GI17506829, Length=480, Percent_Identity=46.25, Blast_Score=352, Evalue=3e-97,
Organism=Saccharomyces cerevisiae, GI6319279, Length=481, Percent_Identity=44.6985446985447, Blast_Score=348, Evalue=1e-96,
Organism=Saccharomyces cerevisiae, GI6324923, Length=483, Percent_Identity=43.6853002070393, Blast_Score=345, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=47.6482617586912, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI28571814, Length=489, Percent_Identity=47.6482617586912, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24648966, Length=446, Percent_Identity=46.1883408071749, Blast_Score=345, Evalue=3e-95,
Organism=Drosophila melanogaster, GI24581235, Length=481, Percent_Identity=35.1351351351351, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24646914, Length=274, Percent_Identity=46.7153284671533, Blast_Score=211, Evalue=7e-55,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50528; Mature: 50528

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTKIVCTIGPKTESEEMLTNLLNAGMNVMRLNFSHGDYEEHGNRIKNMRAVMAKTGQN
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCC
AGILLDTKGPEIRTMKLEGGKDAALVAGQTFTFTTDQSVIGNNERVAVTYAGFSADLKIG
CCEEEECCCCCEEEEEECCCCCEEEEECCEEEEECCCHHCCCCCEEEEEEECCEECEEEC
NTVLVDDGLIGMEVTNVTESEVICKVLNSGDLGENKGVNLPGVSIQLPALAEKDKRDLIF
CEEEEECCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEE
GCEQGVDFVAASFIRKRSDVLEIREHLKAHGGSQIQIISKIENQEGLNNFDEILEASDGI
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHCCCCE
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHHCCHHEEEEEHHHHHHHHHCCCCCCCCCHHHHHH
AILDGTDAVMLSGESAKGKYPLEAVTIMATICERTDRVMPSRIDSLHDNRKLRITEAVCR
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEHHHHHH
GAVETAEKLDAPLIVVATSGGKSAKSVRKYFPNAVILALTTNETTAHQLILTKGVIPQMV
HHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCEEEEEEECCCCCHHHEEHHHCHHHHHH
KEIASTDDFYRIGKEAALASGLAQKGDVVVMVSGALVPSGTTNTASVHVL
HHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEECEECCCCCCCCCEEEEC
>Mature Secondary Structure
MKKTKIVCTIGPKTESEEMLTNLLNAGMNVMRLNFSHGDYEEHGNRIKNMRAVMAKTGQN
CCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCC
AGILLDTKGPEIRTMKLEGGKDAALVAGQTFTFTTDQSVIGNNERVAVTYAGFSADLKIG
CCEEEECCCCCEEEEEECCCCCEEEEECCEEEEECCCHHCCCCCEEEEEEECCEECEEEC
NTVLVDDGLIGMEVTNVTESEVICKVLNSGDLGENKGVNLPGVSIQLPALAEKDKRDLIF
CEEEEECCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEE
GCEQGVDFVAASFIRKRSDVLEIREHLKAHGGSQIQIISKIENQEGLNNFDEILEASDGI
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHCCCCE
MVARGDLGVEIPVEEVIFAQKMMIEKCNRARKVVITATQMLDSMIKNPRPTRAEAGDVAN
EEEECCCCCCCCHHHHHHHHHHHHHHHCCHHEEEEEHHHHHHHHHCCCCCCCCCHHHHHH
AILDGTDAVMLSGESAKGKYPLEAVTIMATICERTDRVMPSRIDSLHDNRKLRITEAVCR
HHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEHHHHHH
GAVETAEKLDAPLIVVATSGGKSAKSVRKYFPNAVILALTTNETTAHQLILTKGVIPQMV
HHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCEEEEEEECCCCCHHHEEHHHCHHHHHH
KEIASTDDFYRIGKEAALASGLAQKGDVVVMVSGALVPSGTTNTASVHVL
HHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEECEECCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]