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The map label for this gene is emtA [H]

Identifier: 157370375

GI number: 157370375

Start: 2329858

End: 2330559

Strand: Direct

Name: emtA [H]

Synonym: Spro_2135

Alternate gene names: 157370375

Gene position: 2329858-2330559 (Clockwise)

Preceding gene: 157370369

Following gene: 157370376

Centisome position: 42.76

GC content: 52.14

Gene sequence:

>702_bases
ATGCTACGCAACTCATTGAAAGGAGTGAGGGATTCTGTGAAAACAAAAATAGGTTGCCTGACGGCAATTTTGCTTTTGTC
GGGTTGTGCCAAAGACCCGCAAACAGCAACCAATATTTCAGGCAGTGGCACATCGCGTGGCGGTTGGTTAAAGACTCCCC
CGCAGGCTCCGGTTAACCGTACAGGGACACCGATAGCCTATAACGATTACATTCGTCAGGCCGCTAACAGCTATGGCGTT
GACGAAACGCTGATTAAAGCGATTATCCAGGTGGAATCCGGGTTTAACCCGAATGCAGTCAGCACCTCGAACGCAGTGGG
ATTAATGCAGCTGAAACCTTCAACCGCCGGGCGTGACGCCTATCGTCTGAAAGGAAGAAGCGGGCAGCCAAGCTCGCGTG
AGTTGAAGGATCCGGCGGTTAATATTGATCTGGGAACGGCCTATATCAATATTCTTCAAAGCCAGCAGCTAGCCGGGATC
AACGATCCACAGACGTTACGTTACGCCACCATTGTTTCTTATGTGAATGGAGCGGGCGCCATGCTACGGACCTTCTCATC
GGACAAGCGCGTAGCGGTGAATCGTATCAATCAGATGAGCCCGGATGAGTTCTATCAGCATATCCAGAAAAAGCATCCGG
CCCCGCAGGCGCCGCGCTATTTATGGAAGGTGACCACCGCCTACCAGGCAATGTCACCCTAG

Upstream 100 bases:

>100_bases
AAAACACGTTGTTGCAGATTGACTCATAAATCACCTCTTGGTATACGAGTAGCTTGAGATTAGACCAAAAAAAGCGTACA
GGCGTCCTTACAAGTTGTAT

Downstream 100 bases:

>100_bases
TTCAGATTTCTTTGCTAAACCGGCCTCTTAATGGGGCCGGTTTTTTTATTGGCGCGAGGTAAAATCGCGATGCACTTCTC
ATTTTTCGTTATTTCCCATC

Product: lytic transglycosylase

Products: NA

Alternate protein names: Peptidoglycan lytic endotransglycosylase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MLRNSLKGVRDSVKTKIGCLTAILLLSGCAKDPQTATNISGSGTSRGGWLKTPPQAPVNRTGTPIAYNDYIRQAANSYGV
DETLIKAIIQVESGFNPNAVSTSNAVGLMQLKPSTAGRDAYRLKGRSGQPSSRELKDPAVNIDLGTAYINILQSQQLAGI
NDPQTLRYATIVSYVNGAGAMLRTFSSDKRVAVNRINQMSPDEFYQHIQKKHPAPQAPRYLWKVTTAYQAMSP

Sequences:

>Translated_233_residues
MLRNSLKGVRDSVKTKIGCLTAILLLSGCAKDPQTATNISGSGTSRGGWLKTPPQAPVNRTGTPIAYNDYIRQAANSYGV
DETLIKAIIQVESGFNPNAVSTSNAVGLMQLKPSTAGRDAYRLKGRSGQPSSRELKDPAVNIDLGTAYINILQSQQLAGI
NDPQTLRYATIVSYVNGAGAMLRTFSSDKRVAVNRINQMSPDEFYQHIQKKHPAPQAPRYLWKVTTAYQAMSP
>Mature_233_residues
MLRNSLKGVRDSVKTKIGCLTAILLLSGCAKDPQTATNISGSGTSRGGWLKTPPQAPVNRTGTPIAYNDYIRQAANSYGV
DETLIKAIIQVESGFNPNAVSTSNAVGLMQLKPSTAGRDAYRLKGRSGQPSSRELKDPAVNIDLGTAYINILQSQQLAGI
NDPQTLRYATIVSYVNGAGAMLRTFSSDKRVAVNRINQMSPDEFYQHIQKKHPAPQAPRYLWKVTTAYQAMSP

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division. Preferentially cleaves at a distance of more than two disaccharide units from the ends of the glycan chain [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87081855, Length=214, Percent_Identity=50.4672897196262, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI87082191, Length=157, Percent_Identity=53.5031847133758, Blast_Score=171, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 25242; Mature: 25242

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRNSLKGVRDSVKTKIGCLTAILLLSGCAKDPQTATNISGSGTSRGGWLKTPPQAPVNR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TGTPIAYNDYIRQAANSYGVDETLIKAIIQVESGFNPNAVSTSNAVGLMQLKPSTAGRDA
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCC
YRLKGRSGQPSSRELKDPAVNIDLGTAYINILQSQQLAGINDPQTLRYATIVSYVNGAGA
EEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHH
MLRTFSSDKRVAVNRINQMSPDEFYQHIQKKHPAPQAPRYLWKVTTAYQAMSP
HHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLRNSLKGVRDSVKTKIGCLTAILLLSGCAKDPQTATNISGSGTSRGGWLKTPPQAPVNR
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TGTPIAYNDYIRQAANSYGVDETLIKAIIQVESGFNPNAVSTSNAVGLMQLKPSTAGRDA
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCC
YRLKGRSGQPSSRELKDPAVNIDLGTAYINILQSQQLAGINDPQTLRYATIVSYVNGAGA
EEECCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHH
MLRTFSSDKRVAVNRINQMSPDEFYQHIQKKHPAPQAPRYLWKVTTAYQAMSP
HHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA