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The map label for this gene is yebU [H]

Identifier: 157370355

GI number: 157370355

Start: 2306082

End: 2307593

Strand: Reverse

Name: yebU [H]

Synonym: Spro_2113

Alternate gene names: 157370355

Gene position: 2307593-2306082 (Counterclockwise)

Preceding gene: 157370356

Following gene: 157370354

Centisome position: 42.35

GC content: 59.72

Gene sequence:

>1512_bases
ATGCCGGGCCCCTACTTTTTTCGGTTTGTGGTAAACTCCCGCCCTTATTTTGATAGCTGCCAGAGAGTTCACCCCGTGGC
CAAACCCGCCTGCGTTTTTTTGCCGCCCGCCTTTCTCGAGGCGACGCGCGCCATCATGCCCGCCGACCTGTCGATGGACG
ATTTCATTGCCGCCTGCCAGCGCCCGCTGCGCCGCAGTCTGCGAGTTAATACGCTAAAAATCAGCGTCACCGACTTTCTG
ACGCTGGTGCAGGATTATGACTGGCGTCTGGAGCCCATCCCCTGGTGCGCGGAAGGCTTTTGGATCGAACGCGGGGACGA
AGAACTGCGGCTGGGCAGCGCTGCTGAACACCTGAGCGGCCTGTTTTACATTCAGGAGGCCAGCTCAATGCTGCCGGTCA
GTGCGCTGTTCGCCGGGGCCGAAGCACCAAGCCGGGTACTGGACGTAGCGGCCGCACCAGGATCCAAAACCACCCAGATT
GCGGCGCTGATGAACAATCAGGGCGGCATTGTCGCCAATGAATACTCCGCCAGCCGGGTCAAGGTATTGCACGCCAACAT
TAGCCGCTGTGGCGTGAAGAATGTGGCCCTGACCCACTTCGACGGCCGGGTGTTCGGCGCTGCGTTGCCGGAAAGCTTCG
ACGCCATCTTGCTCGATGCGCCCTGCTCTGGCGAAGGTGTGGTGCGTAAGGATCCGGACGCCATGAGTAACTGGTCACCG
GAAAGCGTGACCGCCATTGCCGACACCCAGCGTGAACTGATCGACAGTGCCTTCCATGCTCTGGCGCCCGGCGGCGTGAT
GGTTTACTCCACCTGCACGCTCAACGCGCAGGAAAATCAGCAGATCGTCAACGGGCTGCTGGCGACCTACGGCGACGCGG
TCAGCATTGAACCGCTGGGTGACTTGTTCCCCGGTGCTAAACAGGCGTTAACCGCCGAAGGTTTTCTGCACGTGTTCCCG
CAAATCTACGATAGCGAAGGCTTCTTCGTGGCACGGTTGCGCAAGCACCACTCCGTCCCCCCGCTGGCAAAGCCAAGCTA
TAAGCTGGGCAAATTCCCGTTTACCCCGCTTTCCGGTAAGGATTCGGCCGAAATCGCTCAGGCAGCAGCGGCCTCCGGAT
TAGCCTGGGATAAAACCAGCCGTCTGTGGGCTCGCGATAAAGAAATCTGGCTGTTCCCTGCCGAGCTGGAAGCGCTGGTT
AATAAAATTCGCTTCTCGCGTATCGGGCTGAAACTGGCCGAGCGTTTCACCAAAGGCTACCGCTGGCAGCATGAAGCGGT
TATCGCACTGGCGGTTGCCGACGGCAAACAGCGTTTTGAACTGGACGCCACGCTGGCGCAGGAGTGGTTCCACGGCCGTG
ACCTCTACCCGGAGCCACCGCCGCAGACCGATGAATGTATTGTCACCTACCAGCAACAGCCGCTGGGTATCGCCAAACGC
ATCGGCAGCCGGATCAAAAACAACCTGCCGCGCGAACTGGTTCGTGACGGCGTGTTGGATTTCCATCAATAA

Upstream 100 bases:

>100_bases
CAAGCACTTTATGCTGAATGCGGAAGAGCCAAAAGACTGGCGTGAATGGGGCACCGCCATCCCTAAAGAGTAAATTCCTC
ACCCTCTCAGGGGCTCGGCA

Downstream 100 bases:

>100_bases
TCCCCTGTCACTGGCCGGAAAACTCCGGCCAGTTAATACCGCTAATAATATTCCGACAGGAGAATATTATTATTCTAATC
ATTTAACCCTCTGGGTATTC

Product: rRNA (cytosine-C(5)-)-methyltransferase RsmF

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MPGPYFFRFVVNSRPYFDSCQRVHPVAKPACVFLPPAFLEATRAIMPADLSMDDFIAACQRPLRRSLRVNTLKISVTDFL
TLVQDYDWRLEPIPWCAEGFWIERGDEELRLGSAAEHLSGLFYIQEASSMLPVSALFAGAEAPSRVLDVAAAPGSKTTQI
AALMNNQGGIVANEYSASRVKVLHANISRCGVKNVALTHFDGRVFGAALPESFDAILLDAPCSGEGVVRKDPDAMSNWSP
ESVTAIADTQRELIDSAFHALAPGGVMVYSTCTLNAQENQQIVNGLLATYGDAVSIEPLGDLFPGAKQALTAEGFLHVFP
QIYDSEGFFVARLRKHHSVPPLAKPSYKLGKFPFTPLSGKDSAEIAQAAAASGLAWDKTSRLWARDKEIWLFPAELEALV
NKIRFSRIGLKLAERFTKGYRWQHEAVIALAVADGKQRFELDATLAQEWFHGRDLYPEPPPQTDECIVTYQQQPLGIAKR
IGSRIKNNLPRELVRDGVLDFHQ

Sequences:

>Translated_503_residues
MPGPYFFRFVVNSRPYFDSCQRVHPVAKPACVFLPPAFLEATRAIMPADLSMDDFIAACQRPLRRSLRVNTLKISVTDFL
TLVQDYDWRLEPIPWCAEGFWIERGDEELRLGSAAEHLSGLFYIQEASSMLPVSALFAGAEAPSRVLDVAAAPGSKTTQI
AALMNNQGGIVANEYSASRVKVLHANISRCGVKNVALTHFDGRVFGAALPESFDAILLDAPCSGEGVVRKDPDAMSNWSP
ESVTAIADTQRELIDSAFHALAPGGVMVYSTCTLNAQENQQIVNGLLATYGDAVSIEPLGDLFPGAKQALTAEGFLHVFP
QIYDSEGFFVARLRKHHSVPPLAKPSYKLGKFPFTPLSGKDSAEIAQAAAASGLAWDKTSRLWARDKEIWLFPAELEALV
NKIRFSRIGLKLAERFTKGYRWQHEAVIALAVADGKQRFELDATLAQEWFHGRDLYPEPPPQTDECIVTYQQQPLGIAKR
IGSRIKNNLPRELVRDGVLDFHQ
>Mature_502_residues
PGPYFFRFVVNSRPYFDSCQRVHPVAKPACVFLPPAFLEATRAIMPADLSMDDFIAACQRPLRRSLRVNTLKISVTDFLT
LVQDYDWRLEPIPWCAEGFWIERGDEELRLGSAAEHLSGLFYIQEASSMLPVSALFAGAEAPSRVLDVAAAPGSKTTQIA
ALMNNQGGIVANEYSASRVKVLHANISRCGVKNVALTHFDGRVFGAALPESFDAILLDAPCSGEGVVRKDPDAMSNWSPE
SVTAIADTQRELIDSAFHALAPGGVMVYSTCTLNAQENQQIVNGLLATYGDAVSIEPLGDLFPGAKQALTAEGFLHVFPQ
IYDSEGFFVARLRKHHSVPPLAKPSYKLGKFPFTPLSGKDSAEIAQAAAASGLAWDKTSRLWARDKEIWLFPAELEALVN
KIRFSRIGLKLAERFTKGYRWQHEAVIALAVADGKQRFELDATLAQEWFHGRDLYPEPPPQTDECIVTYQQQPLGIAKRI
GSRIKNNLPRELVRDGVLDFHQ

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=301, Percent_Identity=34.8837209302326, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI76150623, Length=301, Percent_Identity=34.8837209302326, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI32698918, Length=151, Percent_Identity=39.7350993377483, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI11545785, Length=185, Percent_Identity=35.1351351351351, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI39995082, Length=377, Percent_Identity=25.1989389920424, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI301336155, Length=286, Percent_Identity=26.9230769230769, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI23199998, Length=318, Percent_Identity=29.2452830188679, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI270288818, Length=318, Percent_Identity=29.2452830188679, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI270288816, Length=318, Percent_Identity=29.2452830188679, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI8922322, Length=318, Percent_Identity=29.2452830188679, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI40316918, Length=188, Percent_Identity=30.8510638297872, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI87081985, Length=469, Percent_Identity=69.9360341151386, Blast_Score=685, Evalue=0.0,
Organism=Escherichia coli, GI2367212, Length=217, Percent_Identity=30.8755760368664, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17536757, Length=311, Percent_Identity=31.5112540192926, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI25143471, Length=197, Percent_Identity=31.9796954314721, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71998419, Length=312, Percent_Identity=27.5641025641026, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI115534021, Length=227, Percent_Identity=29.9559471365639, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324268, Length=288, Percent_Identity=35.7638888888889, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6319447, Length=304, Percent_Identity=28.6184210526316, Blast_Score=106, Evalue=1e-23,
Organism=Drosophila melanogaster, GI22024126, Length=307, Percent_Identity=32.8990228013029, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI21355201, Length=320, Percent_Identity=27.1875, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24668781, Length=154, Percent_Identity=36.3636363636364, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356513, Length=154, Percent_Identity=36.3636363636364, Blast_Score=87, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR009072
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 55502; Mature: 55371

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGPYFFRFVVNSRPYFDSCQRVHPVAKPACVFLPPAFLEATRAIMPADLSMDDFIAACQ
CCCCEEEEEEECCCCCHHHHHHCCCCCCCCEEECCHHHHHHHHHHCCCCCCHHHHHHHHH
RPLRRSLRVNTLKISVTDFLTLVQDYDWRLEPIPWCAEGFWIERGDEELRLGSAAEHLSG
HHHHHHHCEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHCCHHHHHHHH
LFYIQEASSMLPVSALFAGAEAPSRVLDVAAAPGSKTTQIAALMNNQGGIVANEYSASRV
EEEEECCCCCCCHHHHHHCCCCCHHHHEEECCCCCCCEEEEEEECCCCCEEEECCCCCEE
KVLHANISRCGVKNVALTHFDGRVFGAALPESFDAILLDAPCSGEGVVRKDPDAMSNWSP
EEEECCHHHCCCCCEEEEEECCEEEECCCCCCCCEEEEECCCCCCCCEECCCHHHCCCCC
ESVTAIADTQRELIDSAFHALAPGGVMVYSTCTLNAQENQQIVNGLLATYGDAVSIEPLG
CHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHHHHCCCCEEECCCH
DLFPGAKQALTAEGFLHVFPQIYDSEGFFVARLRKHHSVPPLAKPSYKLGKFPFTPLSGK
HCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
DSAEIAQAAAASGLAWDKTSRLWARDKEIWLFPAELEALVNKIRFSRIGLKLAERFTKGY
CHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RWQHEAVIALAVADGKQRFELDATLAQEWFHGRDLYPEPPPQTDECIVTYQQQPLGIAKR
CCCCCEEEEEEEECCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHH
IGSRIKNNLPRELVRDGVLDFHQ
HHHHHHHCCCHHHHHCCCCCCCC
>Mature Secondary Structure 
PGPYFFRFVVNSRPYFDSCQRVHPVAKPACVFLPPAFLEATRAIMPADLSMDDFIAACQ
CCCEEEEEEECCCCCHHHHHHCCCCCCCCEEECCHHHHHHHHHHCCCCCCHHHHHHHHH
RPLRRSLRVNTLKISVTDFLTLVQDYDWRLEPIPWCAEGFWIERGDEELRLGSAAEHLSG
HHHHHHHCEEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHCCHHHHHHHH
LFYIQEASSMLPVSALFAGAEAPSRVLDVAAAPGSKTTQIAALMNNQGGIVANEYSASRV
EEEEECCCCCCCHHHHHHCCCCCHHHHEEECCCCCCCEEEEEEECCCCCEEEECCCCCEE
KVLHANISRCGVKNVALTHFDGRVFGAALPESFDAILLDAPCSGEGVVRKDPDAMSNWSP
EEEECCHHHCCCCCEEEEEECCEEEECCCCCCCCEEEEECCCCCCCCEECCCHHHCCCCC
ESVTAIADTQRELIDSAFHALAPGGVMVYSTCTLNAQENQQIVNGLLATYGDAVSIEPLG
CHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHHHHHCCCCEEECCCH
DLFPGAKQALTAEGFLHVFPQIYDSEGFFVARLRKHHSVPPLAKPSYKLGKFPFTPLSGK
HCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
DSAEIAQAAAASGLAWDKTSRLWARDKEIWLFPAELEALVNKIRFSRIGLKLAERFTKGY
CHHHHHHHHHHCCCCCCCCCHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RWQHEAVIALAVADGKQRFELDATLAQEWFHGRDLYPEPPPQTDECIVTYQQQPLGIAKR
CCCCCEEEEEEEECCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCHHHHH
IGSRIKNNLPRELVRDGVLDFHQ
HHHHHHHCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA