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The map label for this gene is puuD [H]

Identifier: 157370309

GI number: 157370309

Start: 2259258

End: 2260019

Strand: Reverse

Name: puuD [H]

Synonym: Spro_2067

Alternate gene names: 157370309

Gene position: 2260019-2259258 (Counterclockwise)

Preceding gene: 157370317

Following gene: 157370308

Centisome position: 41.48

GC content: 58.53

Gene sequence:

>762_bases
ATGGGCAATATATTTAACAGACCAGTGGTCGGCGTAGTGATGTGCAGGTATAGGTTAAACGGGCACCTCACTCAGACCTT
GCAAGAAAAGTACCTAAATGCCGTAGTCGCTGCCGGAGGGCTGCCAATCGCCCTGCCACACGCGCTGGCTGAGCCGAAAC
TGCTGGAAGAATTGCTGGAGCACCTTGACGGTATTGTGCTGCCGGGCAGCCCCAGTAATGTGCAGCCGCACCTTTATGGT
GAAAACGGCGATGAGCCTGACGCCGATCCTGGGCGAGATGAATTGAGCCTGGCGCTGATCCGCCACTCGCTCGACAGGCG
CATTCCCCTTTTCGCCATTTGCCGCGGCATGCAAGAAATGGTGGTCGCCAGCCAGGGCACCCTGCACCGCCGGCTGTTTG
AATTACCCGAACTGCTCGAGCACCGTGAAGATCACGACCTGCCGCTGGAACAACAATACGCCCCCGCCCACGAAGTCATC
GTTCAGGAAGGCGGCCTGCTCTCGCAACTGATACCGGATTGCAACAGATTCTGGGTGAACTCACTGCATGGCCAGGGGGC
AAAAACCCTTGGCCCCGGCGTACGTATTGAGGCCCACGCCGCCGATGGGTTGGTGGAGGCCATCAGCCTGCGCGACCAGC
CTTTCGCGCTGGGTGTGCAATGGCACCCGGAATGGAACAGTGAGGAGTACGCTCTGTCACGCCTGCTGTTTGAGGGGTTC
ATTAGCGCCTGCCGGAACTATCAAAAGGAAAAACGCCCATGA

Upstream 100 bases:

>100_bases
TGAATATTGCCCATTTGACTGCTAGATTTATAAAATATTGAACGAAAACGGCAGTGGGGAAGCTTATCGTTCATAATTTC
GAACATAGCAAGGGGACAGT

Downstream 100 bases:

>100_bases
GCGATGTCAGCTTGGCACCGGGAAAACGCCTGTCGCAAATCCGCCAGCAATTGGGATTGTCACAGCGCCGCGCGGCCGAA
CTGTCTGGATTAACCCACAG

Product: gamma-glutamyl-gamma-aminobutyrate hydrolase

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MGNIFNRPVVGVVMCRYRLNGHLTQTLQEKYLNAVVAAGGLPIALPHALAEPKLLEELLEHLDGIVLPGSPSNVQPHLYG
ENGDEPDADPGRDELSLALIRHSLDRRIPLFAICRGMQEMVVASQGTLHRRLFELPELLEHREDHDLPLEQQYAPAHEVI
VQEGGLLSQLIPDCNRFWVNSLHGQGAKTLGPGVRIEAHAADGLVEAISLRDQPFALGVQWHPEWNSEEYALSRLLFEGF
ISACRNYQKEKRP

Sequences:

>Translated_253_residues
MGNIFNRPVVGVVMCRYRLNGHLTQTLQEKYLNAVVAAGGLPIALPHALAEPKLLEELLEHLDGIVLPGSPSNVQPHLYG
ENGDEPDADPGRDELSLALIRHSLDRRIPLFAICRGMQEMVVASQGTLHRRLFELPELLEHREDHDLPLEQQYAPAHEVI
VQEGGLLSQLIPDCNRFWVNSLHGQGAKTLGPGVRIEAHAADGLVEAISLRDQPFALGVQWHPEWNSEEYALSRLLFEGF
ISACRNYQKEKRP
>Mature_252_residues
GNIFNRPVVGVVMCRYRLNGHLTQTLQEKYLNAVVAAGGLPIALPHALAEPKLLEELLEHLDGIVLPGSPSNVQPHLYGE
NGDEPDADPGRDELSLALIRHSLDRRIPLFAICRGMQEMVVASQGTLHRRLFELPELLEHREDHDLPLEQQYAPAHEVIV
QEGGLLSQLIPDCNRFWVNSLHGQGAKTLGPGVRIEAHAADGLVEAISLRDQPFALGVQWHPEWNSEEYALSRLLFEGFI
SACRNYQKEKRP

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=252, Percent_Identity=74.2063492063492, Blast_Score=395, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 28256; Mature: 28124

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNIFNRPVVGVVMCRYRLNGHLTQTLQEKYLNAVVAAGGLPIALPHALAEPKLLEELLE
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEECHHHHCCHHHHHHHHH
HLDGIVLPGSPSNVQPHLYGENGDEPDADPGRDELSLALIRHSLDRRIPLFAICRGMQEM
HHCCEEECCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VVASQGTLHRRLFELPELLEHREDHDLPLEQQYAPAHEVIVQEGGLLSQLIPDCNRFWVN
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHH
SLHGQGAKTLGPGVRIEAHAADGLVEAISLRDQPFALGVQWHPEWNSEEYALSRLLFEGF
HHCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
ISACRNYQKEKRP
HHHHHHHHHHCCC
>Mature Secondary Structure 
GNIFNRPVVGVVMCRYRLNGHLTQTLQEKYLNAVVAAGGLPIALPHALAEPKLLEELLE
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEECHHHHCCHHHHHHHHH
HLDGIVLPGSPSNVQPHLYGENGDEPDADPGRDELSLALIRHSLDRRIPLFAICRGMQEM
HHCCEEECCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VVASQGTLHRRLFELPELLEHREDHDLPLEQQYAPAHEVIVQEGGLLSQLIPDCNRFWVN
HHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHH
SLHGQGAKTLGPGVRIEAHAADGLVEAISLRDQPFALGVQWHPEWNSEEYALSRLLFEGF
HHCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHH
ISACRNYQKEKRP
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]