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The map label for this gene is puuB [H]

Identifier: 157370306

GI number: 157370306

Start: 2255900

End: 2257180

Strand: Reverse

Name: puuB [H]

Synonym: Spro_2064

Alternate gene names: 157370306

Gene position: 2257180-2255900 (Counterclockwise)

Preceding gene: 157370307

Following gene: 157370304

Centisome position: 41.42

GC content: 57.22

Gene sequence:

>1281_bases
ATGACTGAACATGTAAAAAGCTATTATGCGGCCTCGGCCAACAGCCACGCGCCCTACCCGCAGTTGAATGACTCTATTGA
ATGCGACGTCTGCATCGTCGGCGGTGGCTATACCGGTCTGTCTTCCGCACTGTTTCTGGTAGAAGCCGGCTATAACGTGG
TGGTGCTGGAATCGGCGCGCATCGGTTTCGGCGCCAGCGGCCGTAACGGCGGCCAACTGGTCAACTCTTACAGCCGCGAT
ATTGACGTGATTGAACAACGCTATGGGGCAGAAAGCGCCCGCGTTCTCGGCAGTATGATGTTTGAGGGCGCAGAGATCAT
TCGCAGCCGTATCCAAAGGTACGCCATCGAATGTGACTATCGCCCCGGCGGCATCTTCGCCGCACTGAACAACCGGCAAT
ACCATGCGTTGGTCGAGCAGAAACAGAACTGGGAACGCTACGGTAATACTCAACTTGAGCTGCTGGACGCCGGGCGTATC
CGTCAAGAGGTCAATAGCGAGCGCTACGTCGGCGCGCTGCTGGATCATAGCGGTGGTCATATTCATCCATTGAATCTGGC
ACTCGGGGAAGCCGAAGCCATTCGCCTGCAGGGTGGGCGGATCTTCGAACAATCGGCCGTGACCAACATTCGCCACGGCG
AACCGGCGATGGTCAGTACCGCTAATGGGCAGGTGAAGGCGCGTTATGTGATCGTGGCCGGTAACGCTTATCTGGGCGAT
AAGCTGGAGCCAAGACTGGCCAAATGCAGCATGCCGTGCGGCACCCAGGTGGTTGCCACCGAGCCGCTGGCACCGGAAGT
GGCGCAGTCGCTGATCCCACAAAATTACTGCGTCGAGGACTGCAACTATCTGCTGGATTATTATCGTATAACCGGTGATA
ACCGCCTGCTGTACGGCGGTGGTGTGGTGTACGGTGCCCGCGATCCGGACGATATCGACAATCTGATCCGCCCGAAACTG
CTGAAAACCTTCCCACAGTTAAAAGGGGTAAATATCGACTACCGCTGGACCGGCAATTTCCTGCTGACGCTGTCGCGTAT
GCCACAGTTCGGCCGACTGGAAAACAACGTCTATTACATGCAGGGCTACAGCGGCCACGGCGTGACCTGTACCCATCTGG
CGGGTAAATTGATTGCCGAGTTAATGCAAGGCGATGCGGAACGCTTCGATGCCTTTGCCAAACTGCCGCACCTGCCGTTC
TTCGGTGGCCGCAACCTGCAAATTCCGTTTACCGCCATCGGCGCGGCCTACTACACCCTGCGCGATCGGATCGGGGTGTA
A

Upstream 100 bases:

>100_bases
ACAAGCAAAGCGGCAACGGTCGCGACAAATCGCTGCACGCGCTGGAAAAATTCACCGAACTGAAAACCACCTGGATTTCG
CTGGAATAAGGGCAAATATC

Downstream 100 bases:

>100_bases
AGCACGCAGGCCCGGCGTTTAGCCTTGCCGGGCCTGATGATTAACGCTTAAACAATCTTCGGTTGGCCGTCTGAGGCAGT
CGCCCCACCGTCAACCGGGA

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTEHVKSYYAASANSHAPYPQLNDSIECDVCIVGGGYTGLSSALFLVEAGYNVVVLESARIGFGASGRNGGQLVNSYSRD
IDVIEQRYGAESARVLGSMMFEGAEIIRSRIQRYAIECDYRPGGIFAALNNRQYHALVEQKQNWERYGNTQLELLDAGRI
RQEVNSERYVGALLDHSGGHIHPLNLALGEAEAIRLQGGRIFEQSAVTNIRHGEPAMVSTANGQVKARYVIVAGNAYLGD
KLEPRLAKCSMPCGTQVVATEPLAPEVAQSLIPQNYCVEDCNYLLDYYRITGDNRLLYGGGVVYGARDPDDIDNLIRPKL
LKTFPQLKGVNIDYRWTGNFLLTLSRMPQFGRLENNVYYMQGYSGHGVTCTHLAGKLIAELMQGDAERFDAFAKLPHLPF
FGGRNLQIPFTAIGAAYYTLRDRIGV

Sequences:

>Translated_426_residues
MTEHVKSYYAASANSHAPYPQLNDSIECDVCIVGGGYTGLSSALFLVEAGYNVVVLESARIGFGASGRNGGQLVNSYSRD
IDVIEQRYGAESARVLGSMMFEGAEIIRSRIQRYAIECDYRPGGIFAALNNRQYHALVEQKQNWERYGNTQLELLDAGRI
RQEVNSERYVGALLDHSGGHIHPLNLALGEAEAIRLQGGRIFEQSAVTNIRHGEPAMVSTANGQVKARYVIVAGNAYLGD
KLEPRLAKCSMPCGTQVVATEPLAPEVAQSLIPQNYCVEDCNYLLDYYRITGDNRLLYGGGVVYGARDPDDIDNLIRPKL
LKTFPQLKGVNIDYRWTGNFLLTLSRMPQFGRLENNVYYMQGYSGHGVTCTHLAGKLIAELMQGDAERFDAFAKLPHLPF
FGGRNLQIPFTAIGAAYYTLRDRIGV
>Mature_425_residues
TEHVKSYYAASANSHAPYPQLNDSIECDVCIVGGGYTGLSSALFLVEAGYNVVVLESARIGFGASGRNGGQLVNSYSRDI
DVIEQRYGAESARVLGSMMFEGAEIIRSRIQRYAIECDYRPGGIFAALNNRQYHALVEQKQNWERYGNTQLELLDAGRIR
QEVNSERYVGALLDHSGGHIHPLNLALGEAEAIRLQGGRIFEQSAVTNIRHGEPAMVSTANGQVKARYVIVAGNAYLGDK
LEPRLAKCSMPCGTQVVATEPLAPEVAQSLIPQNYCVEDCNYLLDYYRITGDNRLLYGGGVVYGARDPDDIDNLIRPKLL
KTFPQLKGVNIDYRWTGNFLLTLSRMPQFGRLENNVYYMQGYSGHGVTCTHLAGKLIAELMQGDAERFDAFAKLPHLPFF
GGRNLQIPFTAIGAAYYTLRDRIGV

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=426, Percent_Identity=77.9342723004695, Blast_Score=714, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47055; Mature: 46924

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHVKSYYAASANSHAPYPQLNDSIECDVCIVGGGYTGLSSALFLVEAGYNVVVLESAR
CCCHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHEEEEEECCCCEEEEECCC
IGFGASGRNGGQLVNSYSRDIDVIEQRYGAESARVLGSMMFEGAEIIRSRIQRYAIECDY
CCCCCCCCCCHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
RPGGIFAALNNRQYHALVEQKQNWERYGNTQLELLDAGRIRQEVNSERYVGALLDHSGGH
CCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCEEEEEEECCCCE
IHPLNLALGEAEAIRLQGGRIFEQSAVTNIRHGEPAMVSTANGQVKARYVIVAGNAYLGD
EEEEEEEECCCEEEEECCCEEEHHHHHCCCCCCCCCEEEECCCCEEEEEEEEECCCEECC
KLEPRLAKCSMPCGTQVVATEPLAPEVAQSLIPQNYCVEDCNYLLDYYRITGDNRLLYGG
CCCCHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHEEEECCCEEEEEC
GVVYGARDPDDIDNLIRPKLLKTFPQLKGVNIDYRWTGNFLLTLSRMPQFGRLENNVYYM
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCEEEE
QGYSGHGVTCTHLAGKLIAELMQGDAERFDAFAKLPHLPFFGGRNLQIPFTAIGAAYYTL
ECCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHH
RDRIGV
HHHCCC
>Mature Secondary Structure 
TEHVKSYYAASANSHAPYPQLNDSIECDVCIVGGGYTGLSSALFLVEAGYNVVVLESAR
CCHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHEEEEEECCCCEEEEECCC
IGFGASGRNGGQLVNSYSRDIDVIEQRYGAESARVLGSMMFEGAEIIRSRIQRYAIECDY
CCCCCCCCCCHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
RPGGIFAALNNRQYHALVEQKQNWERYGNTQLELLDAGRIRQEVNSERYVGALLDHSGGH
CCCCEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHCCCCEEEEEEECCCCE
IHPLNLALGEAEAIRLQGGRIFEQSAVTNIRHGEPAMVSTANGQVKARYVIVAGNAYLGD
EEEEEEEECCCEEEEECCCEEEHHHHHCCCCCCCCCEEEECCCCEEEEEEEEECCCEECC
KLEPRLAKCSMPCGTQVVATEPLAPEVAQSLIPQNYCVEDCNYLLDYYRITGDNRLLYGG
CCCCHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHEEEECCCEEEEEC
GVVYGARDPDDIDNLIRPKLLKTFPQLKGVNIDYRWTGNFLLTLSRMPQFGRLENNVYYM
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCEEEE
QGYSGHGVTCTHLAGKLIAELMQGDAERFDAFAKLPHLPFFGGRNLQIPFTAIGAAYYTL
ECCCCCCEEHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHH
RDRIGV
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]