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The map label for this gene is ebgR [H]

Identifier: 157370214

GI number: 157370214

Start: 2157328

End: 2158308

Strand: Direct

Name: ebgR [H]

Synonym: Spro_1972

Alternate gene names: 157370214

Gene position: 2157328-2158308 (Clockwise)

Preceding gene: 157370211

Following gene: 157370215

Centisome position: 39.59

GC content: 53.92

Gene sequence:

>981_bases
ATGGCCACACTGAAGGAAATCGCAGAAGCTGCGAACGTGTCTGTTGCTACCGTTTCGCGAGTGCTGAATGACGACCCCAC
TCTGAGCGTGAAAGCGCAAACCCGACAAAAAATTCTGGAAGCCGCAGAGCGTCTCGAATACAAGGTTCAGCCTTCAAAAC
GTCACAGCGGGCATCGGCTGACGTTTGCCGCGTTGTTCACCTATAAGCAGGGCATCGAGATTAACGATCCTTACTATCTG
GCGATGCGCTATGGCATTGAAACTCAATGTGAAAAACTGGGTATTTCGCTGGTGGCCTGTTACGACTTTAACGGTGAAAA
AGCGCTGCCTGCGGCTGATGGCCTGCTGGTTATCGGTCGCCCTCAGCCGGTGTTGCACGAGCAACTTGGCCAACAGAAAG
TGCCGGTGGTGTTCCTTGACGGTGTGGTTGAAGACCCGCAGTTCGACTGCGTCAACGTTGATTTGTATAAGATCAGCCAG
AAGGTAATCGATTATTTCATCGGGCGCGGTTATTCACGCATTGGGTTTATCGGTGGGCGTGACGATCCGGCCTTTGCCGA
TCAGCGCGAGCAGGCATTTGTCGAGTATGGCCGGTTGAAAAACGTGGTGCAGCCCCAGGATATTTACATCGGTGATTTCA
GCAGTCAGGCGGGTTACCAGTGCGCCAAACGAATGCTGGAGGACACCAGCGATTATCCGCCGGCGCTGTTGGTGGCGACC
GACTCTATTGCCATCGGCGTCCTGCGTGCACTACATGAAAAGGGCATCCAGGTCCCCGGGCAGATTGCGTTGATCAGTGT
GAACGATATTCCCACCGCCCGGTTTATTTTCCCTGCCTTATCAACGGTTCGCATTCCATCAGAAACCCTGGGCGCTCAGG
CCGTCAACCTGTTGATGGAGCGCATTCGCGATGAGCGAACCATTCCTTTATCGATCTTTGTTCCCAGTTCGCTTCAGTTG
CGCGACACCACGCTAGCCTGA

Upstream 100 bases:

>100_bases
GGATGCTTCTGGCGGATAAAAAGGGGGGATGGGGCATCAGGGCGTATACCTTGCTGAGCCGTCCCGGTAAGCTAGCGTTT
TGTCTATCCATTGAGACTCT

Downstream 100 bases:

>100_bases
TTAATCTTCCCTGTGCGGCACGCCGATGGCGGCTGCACAGGGAGGCCCGCCAGAGTAAAAATTAACGTCATGATTAAAAA
AGATATTTAATATTTTTTGT

Product: DNA-binding transcriptional repressor EbgR

Products: NA

Alternate protein names: Ebg operon repressor [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MATLKEIAEAANVSVATVSRVLNDDPTLSVKAQTRQKILEAAERLEYKVQPSKRHSGHRLTFAALFTYKQGIEINDPYYL
AMRYGIETQCEKLGISLVACYDFNGEKALPAADGLLVIGRPQPVLHEQLGQQKVPVVFLDGVVEDPQFDCVNVDLYKISQ
KVIDYFIGRGYSRIGFIGGRDDPAFADQREQAFVEYGRLKNVVQPQDIYIGDFSSQAGYQCAKRMLEDTSDYPPALLVAT
DSIAIGVLRALHEKGIQVPGQIALISVNDIPTARFIFPALSTVRIPSETLGAQAVNLLMERIRDERTIPLSIFVPSSLQL
RDTTLA

Sequences:

>Translated_326_residues
MATLKEIAEAANVSVATVSRVLNDDPTLSVKAQTRQKILEAAERLEYKVQPSKRHSGHRLTFAALFTYKQGIEINDPYYL
AMRYGIETQCEKLGISLVACYDFNGEKALPAADGLLVIGRPQPVLHEQLGQQKVPVVFLDGVVEDPQFDCVNVDLYKISQ
KVIDYFIGRGYSRIGFIGGRDDPAFADQREQAFVEYGRLKNVVQPQDIYIGDFSSQAGYQCAKRMLEDTSDYPPALLVAT
DSIAIGVLRALHEKGIQVPGQIALISVNDIPTARFIFPALSTVRIPSETLGAQAVNLLMERIRDERTIPLSIFVPSSLQL
RDTTLA
>Mature_325_residues
ATLKEIAEAANVSVATVSRVLNDDPTLSVKAQTRQKILEAAERLEYKVQPSKRHSGHRLTFAALFTYKQGIEINDPYYLA
MRYGIETQCEKLGISLVACYDFNGEKALPAADGLLVIGRPQPVLHEQLGQQKVPVVFLDGVVEDPQFDCVNVDLYKISQK
VIDYFIGRGYSRIGFIGGRDDPAFADQREQAFVEYGRLKNVVQPQDIYIGDFSSQAGYQCAKRMLEDTSDYPPALLVATD
SIAIGVLRALHEKGIQVPGQIALISVNDIPTARFIFPALSTVRIPSETLGAQAVNLLMERIRDERTIPLSIFVPSSLQLR
DTTLA

Specific function: Repressor for beta galactosidase alpha and beta subunits (ebgA and ebgC). Binds lactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789456, Length=327, Percent_Identity=55.3516819571865, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=29.4294294294294, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790194, Length=319, Percent_Identity=28.2131661442006, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1790369, Length=337, Percent_Identity=27.0029673590504, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1789202, Length=335, Percent_Identity=27.4626865671642, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1786540, Length=338, Percent_Identity=26.6272189349112, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789068, Length=304, Percent_Identity=26.6447368421053, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788474, Length=316, Percent_Identity=24.6835443037975, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1787580, Length=340, Percent_Identity=26.1764705882353, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36091; Mature: 35960

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLKEIAEAANVSVATVSRVLNDDPTLSVKAQTRQKILEAAERLEYKVQPSKRHSGHRL
CCCHHHHHHHHCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCEEECCCCCCCCCCEE
TFAALFTYKQGIEINDPYYLAMRYGIETQCEKLGISLVACYDFNGEKALPAADGLLVIGR
EEEEEEHHHCCCCCCCCEEEEEECCCHHHHHHCCEEEEEEEECCCCCCCCCCCCEEEEEC
PQPVLHEQLGQQKVPVVFLDGVVEDPQFDCVNVDLYKISQKVIDYFIGRGYSRIGFIGGR
CCHHHHHHCCCCCCCEEEECCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCCEEEEECCC
DDPAFADQREQAFVEYGRLKNVVQPQDIYIGDFSSQAGYQCAKRMLEDTSDYPPALLVAT
CCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEE
DSIAIGVLRALHEKGIQVPGQIALISVNDIPTARFIFPALSTVRIPSETLGAQAVNLLME
CHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHEECCHHHHHHHHHHHHHH
RIRDERTIPLSIFVPSSLQLRDTTLA
HHCCCCCCEEEEEECCCCEECCCCCC
>Mature Secondary Structure 
ATLKEIAEAANVSVATVSRVLNDDPTLSVKAQTRQKILEAAERLEYKVQPSKRHSGHRL
CCHHHHHHHHCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCEEECCCCCCCCCCEE
TFAALFTYKQGIEINDPYYLAMRYGIETQCEKLGISLVACYDFNGEKALPAADGLLVIGR
EEEEEEHHHCCCCCCCCEEEEEECCCHHHHHHCCEEEEEEEECCCCCCCCCCCCEEEEEC
PQPVLHEQLGQQKVPVVFLDGVVEDPQFDCVNVDLYKISQKVIDYFIGRGYSRIGFIGGR
CCHHHHHHCCCCCCCEEEECCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCCEEEEECCC
DDPAFADQREQAFVEYGRLKNVVQPQDIYIGDFSSQAGYQCAKRMLEDTSDYPPALLVAT
CCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEEE
DSIAIGVLRALHEKGIQVPGQIALISVNDIPTARFIFPALSTVRIPSETLGAQAVNLLME
CHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHEECCHHHHHHHHHHHHHH
RIRDERTIPLSIFVPSSLQLRDTTLA
HHCCCCCCEEEEEECCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2515108; 3939708; 9278503 [H]