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The map label for this gene is htrB [H]

Identifier: 157370126

GI number: 157370126

Start: 2069981

End: 2070901

Strand: Reverse

Name: htrB [H]

Synonym: Spro_1884

Alternate gene names: 157370126

Gene position: 2070901-2069981 (Counterclockwise)

Preceding gene: 157370127

Following gene: 157370125

Centisome position: 38.01

GC content: 56.03

Gene sequence:

>921_bases
ATGACTCAAGTTCCTTCTTTTAATCGCTCTTTACTGCATCCGCGTTATTGGCTCACCTGGCTCGGCATTGGTTTGCTTTA
CCTGTTGGTCTTGCTGCCTTATCCGATTATCTATCGCCTCGGCACCTCGCTTGGCCGTTTTTCCATGCGATTTCTCAAGC
GCCGCGTGGCAATAGCCCAACGTAATCTGGTGCTGTGCTTCCCGGACATGCCGCAAGCCGAACGCGATGCGTTGGTGGTG
CAAAATTTTGAATCCGTCGGTATGGGCCTGTTTGAAACCGGTATGGCGTGGTTTTGGCCAAACTGGCGTATCGAACGCTG
GTTCCAGGTTAGCGGGCTGGAACATATTCAGAAGGCACGAGACAACCATCAGGGGGTTTTGCTGATCGGTCTGCACTTCC
TGACGCTGGAACTGGGTGCCCGTATCTTTGGCATTCATAATCCAGGCATCGGCGTTTACCGGCCGCATGACAACAAGCTG
ATGGACTGGCTGCAGACCTGGGGCCGCATGCGTTCCAACAAATCGATGCTGGACCGTAAGGACGTAAAAGGCATGATCCG
CGCGCTAAAGCAGGGGGACATCATCTGGTACGCCCCGGACCATGACTATGGCCCACGCAGCAGCGTGTTCGCCCCACTGT
TCGCCGTAGAGAAAGCCGCCACCACCACCGGCAGCTACGTGCTGGTGAGAATGGGCAAGCCGGCCATTATTCCCTTTACC
CCGCGCCGCCTGCCGGATGCCAAAGGGTATGAGCTGATTATGCAGCCGGCGGTGGAAAACTTCCCGCTGGATAATGAGCT
CGATGCCGCCGCCTTTATGAATAAGGTGGTGGAGAAGGAAATCCTGATGGCGCCGGATCAGTACATGTGGCTGCACCGCC
GCTTCAAAACCCGGCCGGAAGGCGAGCCTTCACTTTACTGA

Upstream 100 bases:

>100_bases
AACGGCAGAAAATAAACGTTTGGTCAAAAATCCTTCGCAGGAGAGACCATTCGAGAACTCTGTTATTATCATTAAATGAC
TGCTTCTAAGCTGAGAACAC

Downstream 100 bases:

>100_bases
CTTCTGACCCGCCGTTTGGCGGGTTAGTTTTTTATGCCGCAGAGCTTGAGAAACGACTGCAACGGTGGGATGGTAAAGCC
TTCGTAGTTCATCCTGGAGC

Product: lipid A biosynthesis lauroyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MTQVPSFNRSLLHPRYWLTWLGIGLLYLLVLLPYPIIYRLGTSLGRFSMRFLKRRVAIAQRNLVLCFPDMPQAERDALVV
QNFESVGMGLFETGMAWFWPNWRIERWFQVSGLEHIQKARDNHQGVLLIGLHFLTLELGARIFGIHNPGIGVYRPHDNKL
MDWLQTWGRMRSNKSMLDRKDVKGMIRALKQGDIIWYAPDHDYGPRSSVFAPLFAVEKAATTTGSYVLVRMGKPAIIPFT
PRRLPDAKGYELIMQPAVENFPLDNELDAAAFMNKVVEKEILMAPDQYMWLHRRFKTRPEGEPSLY

Sequences:

>Translated_306_residues
MTQVPSFNRSLLHPRYWLTWLGIGLLYLLVLLPYPIIYRLGTSLGRFSMRFLKRRVAIAQRNLVLCFPDMPQAERDALVV
QNFESVGMGLFETGMAWFWPNWRIERWFQVSGLEHIQKARDNHQGVLLIGLHFLTLELGARIFGIHNPGIGVYRPHDNKL
MDWLQTWGRMRSNKSMLDRKDVKGMIRALKQGDIIWYAPDHDYGPRSSVFAPLFAVEKAATTTGSYVLVRMGKPAIIPFT
PRRLPDAKGYELIMQPAVENFPLDNELDAAAFMNKVVEKEILMAPDQYMWLHRRFKTRPEGEPSLY
>Mature_305_residues
TQVPSFNRSLLHPRYWLTWLGIGLLYLLVLLPYPIIYRLGTSLGRFSMRFLKRRVAIAQRNLVLCFPDMPQAERDALVVQ
NFESVGMGLFETGMAWFWPNWRIERWFQVSGLEHIQKARDNHQGVLLIGLHFLTLELGARIFGIHNPGIGVYRPHDNKLM
DWLQTWGRMRSNKSMLDRKDVKGMIRALKQGDIIWYAPDHDYGPRSSVFAPLFAVEKAATTTGSYVLVRMGKPAIIPFTP
RRLPDAKGYELIMQPAVENFPLDNELDAAAFMNKVVEKEILMAPDQYMWLHRRFKTRPEGEPSLY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=306, Percent_Identity=68.3006535947712, Blast_Score=456, Evalue=1e-130,
Organism=Escherichia coli, GI87082094, Length=301, Percent_Identity=53.156146179402, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1788161, Length=311, Percent_Identity=29.2604501607717, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35490; Mature: 35359

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQVPSFNRSLLHPRYWLTWLGIGLLYLLVLLPYPIIYRLGTSLGRFSMRFLKRRVAIAQ
CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLVLCFPDMPQAERDALVVQNFESVGMGLFETGMAWFWPNWRIERWFQVSGLEHIQKAR
CCEEEEECCCCCCHHHHHHHHCHHHHCCCHHHCCCCEECCCCCEEHHHHHCCHHHHHHHH
DNHQGVLLIGLHFLTLELGARIFGIHNPGIGVYRPHDNKLMDWLQTWGRMRSNKSMLDRK
CCCCCEEEEHHHHHHHHHCCEEEEEECCCCCEECCCCHHHHHHHHHHHHHHCCHHHHHHH
DVKGMIRALKQGDIIWYAPDHDYGPRSSVFAPLFAVEKAATTTGSYVLVRMGKPAIIPFT
HHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEECCC
PRRLPDAKGYELIMQPAVENFPLDNELDAAAFMNKVVEKEILMAPDQYMWLHRRFKTRPE
CCCCCCCCCCCEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
GEPSLY
CCCCCC
>Mature Secondary Structure 
TQVPSFNRSLLHPRYWLTWLGIGLLYLLVLLPYPIIYRLGTSLGRFSMRFLKRRVAIAQ
CCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLVLCFPDMPQAERDALVVQNFESVGMGLFETGMAWFWPNWRIERWFQVSGLEHIQKAR
CCEEEEECCCCCCHHHHHHHHCHHHHCCCHHHCCCCEECCCCCEEHHHHHCCHHHHHHHH
DNHQGVLLIGLHFLTLELGARIFGIHNPGIGVYRPHDNKLMDWLQTWGRMRSNKSMLDRK
CCCCCEEEEHHHHHHHHHCCEEEEEECCCCCEECCCCHHHHHHHHHHHHHHCCHHHHHHH
DVKGMIRALKQGDIIWYAPDHDYGPRSSVFAPLFAVEKAATTTGSYVLVRMGKPAIIPFT
HHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEECCC
PRRLPDAKGYELIMQPAVENFPLDNELDAAAFMNKVVEKEILMAPDQYMWLHRRFKTRPE
CCCCCCCCCCCEEECCHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCC
GEPSLY
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]