Click here to switch to the map view.

The map label for this gene is leuA [H]

Identifier: 157370117

GI number: 157370117

Start: 2061955

End: 2063625

Strand: Direct

Name: leuA [H]

Synonym: Spro_1875

Alternate gene names: 157370117

Gene position: 2061955-2063625 (Clockwise)

Preceding gene: 157370114

Following gene: 157370119

Centisome position: 37.84

GC content: 59.13

Gene sequence:

>1671_bases
ATGTTAACCAACCCTTCAATCAAATATCGTCCGTTCCCACCGGTGGCTTTGCCGGAGCGTCAGTGGCCGGGGCGCACCTT
GCAGCAGGCACCGCGCTGGTGTTCCAGCGATCTGCGTGATGGCAATCAGGCGCTGGCGGAGCCGATGGATAACGCCCGCA
AGCGCCAATTCTACCAACTGCTGTTGCAGTGCGGGTTCAAAGAGATTGAGGTGGCTTTTCCTTCGGCGTCACAAACCGAC
TTTGATTTTGTCCGCTCGTTGATCGATGAACAGTTGATCCCGGACGATGTCAGCATTCAGGTCTTGACCCAATCGCGTGA
CGACCTGATCGATCGCACCTTTGAGGCACTGCGAGGGGCACCACGGGCTATTGTGCATTTGTATAACGCCACCGCACCGA
TGTTCCGTGACATTGTCTTCAAACAGGACAAGGCGGCGACGGTGGCACTGGCGGTCAATGGCGCGCTGCGTATTTGTCAG
CAGTGCGAGCAGCAGCCGGAAACTGCCTGGACCTTCGAGTATTCGCCGGAAACCTTCTGTTTTACCGAACTGGAGTTTGC
GCTGGAGATTTGCGAAGTGGTGGCGGATGTCTGGCAGCCTGGCCCAGGGCGACCGATGATCATCAACCTGCCGGCGACGG
TGGAAGTCAGTACGCCGAACGTTTATGCCGACCAGATTGAATGGTTTTGTCGGCGCTTCAGTCAGCGAAATCGGGTGACC
ATCAGCGTGCACCCGCATAACGATCGCGGCACCGGGGTGGCGTGTGCCGAACTGGCGATGCTGGCGGGGGCCGATCGGGT
GGAAGGTTGCCTGTTCGGCAACGGCGAGCGCACCGGCAATGTTGACCTGGTGACGTTGGCGCTCAATCTGTACACCCAGG
GCATTGCACCGGGGCTGGACTTCAGCCAGTTGAAGCAGGTGGTGGAGGTGGTGGAGCAGTGCAACCAGCTGCCGGTACAT
CCGCGTCATCCTTATGCCGGTGAACTGGTTTTCACCGCTTTTTCCGGCTCGCACCAGGATGCAATCAAAAAGGGCTTCGC
CGCCCAACAGCAACGGCAGGACGGTCTGTGGCAGGTGCCTTATCTGCCGCTCGATCCGGCGGACGTTGGTTGCAGTTATG
AAGCGGTGATCCGGGTTAACAGCCAGTCAGGCAAAAGCGGAGCGGCCTGGCTGCTGGAACAGAACCATGGGTTGTCGTTA
CCGCGCGGCCTGCAGATTGATTTCAGCAAGGTGGTGCAACAATCTACCGACGGCAGCGGCAAAGAGATGACCACGGCAGA
GCTGTGGCGGCTGTTTCGCCTCAGCTACGGGCTGGTTGAGCAACCGCGCCTGCAATTGCTGAGCTACCAGACCGAGAGCC
ACAGCGTCGAAGCCTACAGCTTCAGCGCCCGGGTGTTGTTCGATGGGCAACAGCAGCGGCTGTTGGGGGTAGGGAACGGT
TTGTTATCCAGCGCGGTAGATGCACTGCGTCAGCGCTTTGGTCTGAGCCTGGCGATAGAGGATTACCACGAGCACACGTT
GGGACATCAGAGCCAGAGTCGCGCCGTTGCCTATATCCGCTGTACGCTAGCGCACGGTGAGGCGGCTTATGGCGTGGGCA
TCGACGTCGATTCCGCCAGCGCCTCGCTGCAGGCACTGTTCAACGTTGCCGCACGCTATTTGGCAAAATAA

Upstream 100 bases:

>100_bases
TGTTGTCCGTTTTGCGACGATGGTTGTCTGCCAGGCGAGAGCAAGCTATTTCCCGGTAGGAAATAATAAGGCATCGCTTA
TGACAGCCCAGGAGAGTGCC

Downstream 100 bases:

>100_bases
GCACTGGGCGCAACGCCTAGCAGGCGACGGAACATGGCGCTGAAAGCACTGGGGCTGTCGTAACCCAGACTCAGCGCCAC
TTCCAGCACCGAATGCCCGG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MLTNPSIKYRPFPPVALPERQWPGRTLQQAPRWCSSDLRDGNQALAEPMDNARKRQFYQLLLQCGFKEIEVAFPSASQTD
FDFVRSLIDEQLIPDDVSIQVLTQSRDDLIDRTFEALRGAPRAIVHLYNATAPMFRDIVFKQDKAATVALAVNGALRICQ
QCEQQPETAWTFEYSPETFCFTELEFALEICEVVADVWQPGPGRPMIINLPATVEVSTPNVYADQIEWFCRRFSQRNRVT
ISVHPHNDRGTGVACAELAMLAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGIAPGLDFSQLKQVVEVVEQCNQLPVH
PRHPYAGELVFTAFSGSHQDAIKKGFAAQQQRQDGLWQVPYLPLDPADVGCSYEAVIRVNSQSGKSGAAWLLEQNHGLSL
PRGLQIDFSKVVQQSTDGSGKEMTTAELWRLFRLSYGLVEQPRLQLLSYQTESHSVEAYSFSARVLFDGQQQRLLGVGNG
LLSSAVDALRQRFGLSLAIEDYHEHTLGHQSQSRAVAYIRCTLAHGEAAYGVGIDVDSASASLQALFNVAARYLAK

Sequences:

>Translated_556_residues
MLTNPSIKYRPFPPVALPERQWPGRTLQQAPRWCSSDLRDGNQALAEPMDNARKRQFYQLLLQCGFKEIEVAFPSASQTD
FDFVRSLIDEQLIPDDVSIQVLTQSRDDLIDRTFEALRGAPRAIVHLYNATAPMFRDIVFKQDKAATVALAVNGALRICQ
QCEQQPETAWTFEYSPETFCFTELEFALEICEVVADVWQPGPGRPMIINLPATVEVSTPNVYADQIEWFCRRFSQRNRVT
ISVHPHNDRGTGVACAELAMLAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGIAPGLDFSQLKQVVEVVEQCNQLPVH
PRHPYAGELVFTAFSGSHQDAIKKGFAAQQQRQDGLWQVPYLPLDPADVGCSYEAVIRVNSQSGKSGAAWLLEQNHGLSL
PRGLQIDFSKVVQQSTDGSGKEMTTAELWRLFRLSYGLVEQPRLQLLSYQTESHSVEAYSFSARVLFDGQQQRLLGVGNG
LLSSAVDALRQRFGLSLAIEDYHEHTLGHQSQSRAVAYIRCTLAHGEAAYGVGIDVDSASASLQALFNVAARYLAK
>Mature_556_residues
MLTNPSIKYRPFPPVALPERQWPGRTLQQAPRWCSSDLRDGNQALAEPMDNARKRQFYQLLLQCGFKEIEVAFPSASQTD
FDFVRSLIDEQLIPDDVSIQVLTQSRDDLIDRTFEALRGAPRAIVHLYNATAPMFRDIVFKQDKAATVALAVNGALRICQ
QCEQQPETAWTFEYSPETFCFTELEFALEICEVVADVWQPGPGRPMIINLPATVEVSTPNVYADQIEWFCRRFSQRNRVT
ISVHPHNDRGTGVACAELAMLAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGIAPGLDFSQLKQVVEVVEQCNQLPVH
PRHPYAGELVFTAFSGSHQDAIKKGFAAQQQRQDGLWQVPYLPLDPADVGCSYEAVIRVNSQSGKSGAAWLLEQNHGLSL
PRGLQIDFSKVVQQSTDGSGKEMTTAELWRLFRLSYGLVEQPRLQLLSYQTESHSVEAYSFSARVLFDGQQQRLLGVGNG
LLSSAVDALRQRFGLSLAIEDYHEHTLGHQSQSRAVAYIRCTLAHGEAAYGVGIDVDSASASLQALFNVAARYLAK

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=363, Percent_Identity=24.7933884297521, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324682, Length=561, Percent_Identity=50.6238859180036, Blast_Score=592, Evalue=1e-170,
Organism=Saccharomyces cerevisiae, GI6324225, Length=560, Percent_Identity=50.8928571428571, Blast_Score=582, Evalue=1e-167,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 61756; Mature: 61756

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTNPSIKYRPFPPVALPERQWPGRTLQQAPRWCSSDLRDGNQALAEPMDNARKRQFYQL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LLQCGFKEIEVAFPSASQTDFDFVRSLIDEQLIPDDVSIQVLTQSRDDLIDRTFEALRGA
HHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCC
PRAIVHLYNATAPMFRDIVFKQDKAATVALAVNGALRICQQCEQQPETAWTFEYSPETFC
CHHHHEECCCCHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHCCCCCEEEEEECCCEEE
FTELEFALEICEVVADVWQPGPGRPMIINLPATVEVSTPNVYADQIEWFCRRFSQRNRVT
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHHHHCCCCCEE
ISVHPHNDRGTGVACAELAMLAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGIAPGLD
EEEECCCCCCCCHHHHHHHHHHCCCHHCEEEECCCCCCCCEEEEEEEEHHHHHCCCCCCC
FSQLKQVVEVVEQCNQLPVHPRHPYAGELVFTAFSGSHQDAIKKGFAAQQQRQDGLWQVP
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEECC
YLPLDPADVGCSYEAVIRVNSQSGKSGAAWLLEQNHGLSLPRGLQIDFSKVVQQSTDGSG
CCCCCCHHCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCC
KEMTTAELWRLFRLSYGLVEQPRLQLLSYQTESHSVEAYSFSARVLFDGQQQRLLGVGNG
CCCCHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCEEEEEEEEEEEECCCHHHHHHCCCH
LLSSAVDALRQRFGLSLAIEDYHEHTLGHQSQSRAVAYIRCTLAHGEAAYGVGIDVDSAS
HHHHHHHHHHHHHCCEEEEHHHHHHHCCCCCCCCEEEEEEEEEECCCEEEEEEEECCCHH
ASLQALFNVAARYLAK
HHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTNPSIKYRPFPPVALPERQWPGRTLQQAPRWCSSDLRDGNQALAEPMDNARKRQFYQL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LLQCGFKEIEVAFPSASQTDFDFVRSLIDEQLIPDDVSIQVLTQSRDDLIDRTFEALRGA
HHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCC
PRAIVHLYNATAPMFRDIVFKQDKAATVALAVNGALRICQQCEQQPETAWTFEYSPETFC
CHHHHEECCCCHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHCCCCCEEEEEECCCEEE
FTELEFALEICEVVADVWQPGPGRPMIINLPATVEVSTPNVYADQIEWFCRRFSQRNRVT
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHHHHCCCCCEE
ISVHPHNDRGTGVACAELAMLAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGIAPGLD
EEEECCCCCCCCHHHHHHHHHHCCCHHCEEEECCCCCCCCEEEEEEEEHHHHHCCCCCCC
FSQLKQVVEVVEQCNQLPVHPRHPYAGELVFTAFSGSHQDAIKKGFAAQQQRQDGLWQVP
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEECC
YLPLDPADVGCSYEAVIRVNSQSGKSGAAWLLEQNHGLSLPRGLQIDFSKVVQQSTDGSG
CCCCCCHHCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHCCCCCC
KEMTTAELWRLFRLSYGLVEQPRLQLLSYQTESHSVEAYSFSARVLFDGQQQRLLGVGNG
CCCCHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCEEEEEEEEEEEECCCHHHHHHCCCH
LLSSAVDALRQRFGLSLAIEDYHEHTLGHQSQSRAVAYIRCTLAHGEAAYGVGIDVDSAS
HHHHHHHHHHHHHCCEEEEHHHHHHHCCCCCCCCEEEEEEEEEECCCEEEEEEEECCCHH
ASLQALFNVAARYLAK
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA