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The map label for this gene is ooxB [H]

Identifier: 157370083

GI number: 157370083

Start: 2026075

End: 2027199

Strand: Direct

Name: ooxB [H]

Synonym: Spro_1841

Alternate gene names: 157370083

Gene position: 2026075-2027199 (Clockwise)

Preceding gene: 157370082

Following gene: 157370084

Centisome position: 37.18

GC content: 62.84

Gene sequence:

>1125_bases
ATGATTGCCGGCAGGCGGGCGGATGCGATTGTGGTGGGGGCCGGCATTATCGGCGCTGCCTGCGCCTGGCGGCTGGCGCA
GCAAGGATTACAAGTTTTGCTGGTGGACAACCAGCGTGCCGGTGCAACGGCGGCCGGTATGGGCCACCTGGTGTGCATGG
ACGATAATCCTGCCGAACTGGCTTTAAGCGCCTATTCATTGCAACTTTGGCGCAGTCTGGTGGATCGTCTACCGGAAGGC
TGCGCCTGGCGTGGCTGCGGTACGCTGTGGCTGGCGGAGCGGGACGACGAAATGGCGGTTGCCGAAGCGAAGCGGCAACG
CATGGCGGAGTACGGTGTGACCAGCGAAACCCTGAACACTGCGCAGGTCGCTGTTCTTGAACCCATGCTGCGGCCCGGCC
TGGTCGGTGGTTTACGCGTGCCGGGGGACGGCATTGTTTATGCGCCCAACGTTGCCCGCTGGTTAGTGGCTGACGCCGGG
CCCTCGATCAGGGTCATTGTGGGTGAAGCCGTCACGCTGGAACAGCAGGCGCTGCTGCTGGCGAGCGGCGAACGTTTAAC
CGCACCGGCGGTGGTGCTGGCCTGTGGTCTACAGGCTGACAGGTTGCTGGAGCAACCCTTGTTACGAGCCAAAAAAGGGC
ATCTGGCGATCACCGATCGCTACCCGCAACGGGTGCACCACCAATTGGTTGAGTTGGGTTATGGTGCCAGCGCACACGCC
AGCGATGGCACTTCGGTGGCGTTTAACGTTCAGGCGCGGCCGACCGGTCAGCTGTTGATTGGCTCCTCCCGCCAGTTTGA
CACCCCAGACAGCACTATCGACATGCCGTTACTGGCCGCCATGCTGACACGCGCCACCACCTTTCTTCCTGCCCTGGCAC
AGATGAACATTATCCGCTGCTGGACCGGCCTGCGCGCCGCCTCCGCCGACGGTCTGCCGCTACTCGGGCCACATCCCCAG
CATCGCTGGTTATGGCTGGCATTGGGGCATGAGGGGCTGGGGGTTACCACCGCGCTGGGCAGCGCCGCGTTGATTGCCGC
GCAGATCCATAATCATCGACCGGAGATTGACGATACCCCTTACCTGGCTGCACGAATGTTCCCTAAGGAGGCGTTGCCGG
TATGA

Upstream 100 bases:

>100_bases
GCGATCGCGTGACGCCGTTTATTCGTGGGCAAGCTTACGTTTGCGCCGACAGCCAACTGCTGCTGGATGAGCGCGATCCC
TTCGTTTGGGGGATCCGCTG

Downstream 100 bases:

>100_bases
AACAAAACCTGCTGACGTTGACCCTCAACGGTGAAAGTCTCTGCGTACCCGAAGGACTTAGCGTCGCCGCAGTGCTCAGC
CTGAGCGGGCTGGATAGCTG

Product: FAD dependent oxidoreductase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: Octopine oxidase subunit B [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MIAGRRADAIVVGAGIIGAACAWRLAQQGLQVLLVDNQRAGATAAGMGHLVCMDDNPAELALSAYSLQLWRSLVDRLPEG
CAWRGCGTLWLAERDDEMAVAEAKRQRMAEYGVTSETLNTAQVAVLEPMLRPGLVGGLRVPGDGIVYAPNVARWLVADAG
PSIRVIVGEAVTLEQQALLLASGERLTAPAVVLACGLQADRLLEQPLLRAKKGHLAITDRYPQRVHHQLVELGYGASAHA
SDGTSVAFNVQARPTGQLLIGSSRQFDTPDSTIDMPLLAAMLTRATTFLPALAQMNIIRCWTGLRAASADGLPLLGPHPQ
HRWLWLALGHEGLGVTTALGSAALIAAQIHNHRPEIDDTPYLAARMFPKEALPV

Sequences:

>Translated_374_residues
MIAGRRADAIVVGAGIIGAACAWRLAQQGLQVLLVDNQRAGATAAGMGHLVCMDDNPAELALSAYSLQLWRSLVDRLPEG
CAWRGCGTLWLAERDDEMAVAEAKRQRMAEYGVTSETLNTAQVAVLEPMLRPGLVGGLRVPGDGIVYAPNVARWLVADAG
PSIRVIVGEAVTLEQQALLLASGERLTAPAVVLACGLQADRLLEQPLLRAKKGHLAITDRYPQRVHHQLVELGYGASAHA
SDGTSVAFNVQARPTGQLLIGSSRQFDTPDSTIDMPLLAAMLTRATTFLPALAQMNIIRCWTGLRAASADGLPLLGPHPQ
HRWLWLALGHEGLGVTTALGSAALIAAQIHNHRPEIDDTPYLAARMFPKEALPV
>Mature_374_residues
MIAGRRADAIVVGAGIIGAACAWRLAQQGLQVLLVDNQRAGATAAGMGHLVCMDDNPAELALSAYSLQLWRSLVDRLPEG
CAWRGCGTLWLAERDDEMAVAEAKRQRMAEYGVTSETLNTAQVAVLEPMLRPGLVGGLRVPGDGIVYAPNVARWLVADAG
PSIRVIVGEAVTLEQQALLLASGERLTAPAVVLACGLQADRLLEQPLLRAKKGHLAITDRYPQRVHHQLVELGYGASAHA
SDGTSVAFNVQARPTGQLLIGSSRQFDTPDSTIDMPLLAAMLTRATTFLPALAQMNIIRCWTGLRAASADGLPLLGPHPQ
HRWLWLALGHEGLGVTTALGSAALIAAQIHNHRPEIDDTPYLAARMFPKEALPV

Specific function: Oxidative cleavage of octopine into L-arginine and pyruvate [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 39880; Mature: 39880

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAGRRADAIVVGAGIIGAACAWRLAQQGLQVLLVDNQRAGATAAGMGHLVCMDDNPAEL
CCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEECCCHHHH
ALSAYSLQLWRSLVDRLPEGCAWRGCGTLWLAERDDEMAVAEAKRQRMAEYGVTSETLNT
HHHHHHHHHHHHHHHHCCCCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCHHHCCH
AQVAVLEPMLRPGLVGGLRVPGDGIVYAPNVARWLVADAGPSIRVIVGEAVTLEQQALLL
HHHHHHHHHHCCCCCCCEECCCCCEEECCCHHEEEEECCCCCEEEEECCEEEEHHHEEEE
ASGERLTAPAVVLACGLQADRLLEQPLLRAKKGHLAITDRYPQRVHHQLVELGYGASAHA
ECCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCCC
SDGTSVAFNVQARPTGQLLIGSSRQFDTPDSTIDMPLLAAMLTRATTFLPALAQMNIIRC
CCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WTGLRAASADGLPLLGPHPQHRWLWLALGHEGLGVTTALGSAALIAAQIHNHRPEIDDTP
HCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHCHHHHHHHHHHCCCCCCCCCC
YLAARMFPKEALPV
CHHHHCCCHHCCCC
>Mature Secondary Structure
MIAGRRADAIVVGAGIIGAACAWRLAQQGLQVLLVDNQRAGATAAGMGHLVCMDDNPAEL
CCCCCCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEECCCHHHH
ALSAYSLQLWRSLVDRLPEGCAWRGCGTLWLAERDDEMAVAEAKRQRMAEYGVTSETLNT
HHHHHHHHHHHHHHHHCCCCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCHHHCCH
AQVAVLEPMLRPGLVGGLRVPGDGIVYAPNVARWLVADAGPSIRVIVGEAVTLEQQALLL
HHHHHHHHHHCCCCCCCEECCCCCEEECCCHHEEEEECCCCCEEEEECCEEEEHHHEEEE
ASGERLTAPAVVLACGLQADRLLEQPLLRAKKGHLAITDRYPQRVHHQLVELGYGASAHA
ECCCCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCCCCC
SDGTSVAFNVQARPTGQLLIGSSRQFDTPDSTIDMPLLAAMLTRATTFLPALAQMNIIRC
CCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WTGLRAASADGLPLLGPHPQHRWLWLALGHEGLGVTTALGSAALIAAQIHNHRPEIDDTP
HCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHCHHHHHHHHHHCCCCCCCCCC
YLAARMFPKEALPV
CHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA