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The map label for this gene is rutR [H]

Identifier: 157370066

GI number: 157370066

Start: 2007119

End: 2007793

Strand: Direct

Name: rutR [H]

Synonym: Spro_1824

Alternate gene names: 157370066

Gene position: 2007119-2007793 (Clockwise)

Preceding gene: 157370059

Following gene: 157370069

Centisome position: 36.84

GC content: 58.37

Gene sequence:

>675_bases
ATGCTGGCCAAAATCTGGTTGTGCCAAGGAGCCGTAGTGAAGCCACAAGCCGTTAAACCCCCTACGCGCCGCTCGCGTGC
GGTAGCCGCAAAACGTGGCGCTATCCTGAATGCGGCACTGGAGTTTTTTTCGCTGTACGGTATTCATGGCACCAGTCTGG
ACCAGGTGGCAGAACGTGCCGACGTGTCCAAAACCAATCTGCTGTATTACTTCCCCTCCAAAGAAGCACTGTATCTGGCG
GTGCTGAAAGATATTCTCGACGTCTGGCTGGCGCCATTGAAGGCGCTGCGCGCCGATCAGGAGCCCCTGCAGGCGATTCG
CGAATATATCCGCCTGAAGCTTGAGGTATCTCGCCAGCACCCGCAGGCCTCACGGCTGTTTTGTCTGGAGATGATCCAGG
GGGCGCCGTTGCTGAAACAAGAGCTGCAGGGCGGGTTGAAGGCGCTGGTGGACGAGAAGTCCGCGATGATTGAGCGCTGG
ATCGCCGCAGGCCATATCGCGGCGGTAGAGCCGCACCATCTGATCTTCATGCTGTGGGCGACCACTCAGCACTACGCAGA
TTTTTGGGTGCAGGTGGAGGCAGTGACCGGCAAGACTCTGGACGATGAGGCGTTTTTTCAGCAGACGGTGGAGAACGTGC
AGCGGGTGATTATCGAGGGTATTCGGCCGCGCTAG

Upstream 100 bases:

>100_bases
AACGGCATGCACAAAAAACAGGCGTAACTGCTCATTAAAGGTGCAGAAAATCACTCGGAAGGGGTAGGTAAAAGCGTGGG
TTTCACCCAATGGCTTTGTT

Downstream 100 bases:

>100_bases
GCGGATGAGAGCAGGGGGATAGCGAGCTATCCCCCGGGGAATGTTAAGCGGTTTTCTTTTTGCGCAGGAACACGTAGGAC
AGACCGAGCACCACAAACCA

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MLAKIWLCQGAVVKPQAVKPPTRRSRAVAAKRGAILNAALEFFSLYGIHGTSLDQVAERADVSKTNLLYYFPSKEALYLA
VLKDILDVWLAPLKALRADQEPLQAIREYIRLKLEVSRQHPQASRLFCLEMIQGAPLLKQELQGGLKALVDEKSAMIERW
IAAGHIAAVEPHHLIFMLWATTQHYADFWVQVEAVTGKTLDDEAFFQQTVENVQRVIIEGIRPR

Sequences:

>Translated_224_residues
MLAKIWLCQGAVVKPQAVKPPTRRSRAVAAKRGAILNAALEFFSLYGIHGTSLDQVAERADVSKTNLLYYFPSKEALYLA
VLKDILDVWLAPLKALRADQEPLQAIREYIRLKLEVSRQHPQASRLFCLEMIQGAPLLKQELQGGLKALVDEKSAMIERW
IAAGHIAAVEPHHLIFMLWATTQHYADFWVQVEAVTGKTLDDEAFFQQTVENVQRVIIEGIRPR
>Mature_224_residues
MLAKIWLCQGAVVKPQAVKPPTRRSRAVAAKRGAILNAALEFFSLYGIHGTSLDQVAERADVSKTNLLYYFPSKEALYLA
VLKDILDVWLAPLKALRADQEPLQAIREYIRLKLEVSRQHPQASRLFCLEMIQGAPLLKQELQGGLKALVDEKSAMIERW
IAAGHIAAVEPHHLIFMLWATTQHYADFWVQVEAVTGKTLDDEAFFQQTVENVQRVIIEGIRPR

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: COG1309

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=208, Percent_Identity=71.6346153846154, Blast_Score=311, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 25296; Mature: 25296

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKIWLCQGAVVKPQAVKPPTRRSRAVAAKRGAILNAALEFFSLYGIHGTSLDQVAERA
CCCEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
DVSKTNLLYYFPSKEALYLAVLKDILDVWLAPLKALRADQEPLQAIREYIRLKLEVSRQH
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
PQASRLFCLEMIQGAPLLKQELQGGLKALVDEKSAMIERWIAAGHIAAVEPHHLIFMLWA
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEEEEH
TTQHYADFWVQVEAVTGKTLDDEAFFQQTVENVQRVIIEGIRPR
HHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLAKIWLCQGAVVKPQAVKPPTRRSRAVAAKRGAILNAALEFFSLYGIHGTSLDQVAERA
CCCEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
DVSKTNLLYYFPSKEALYLAVLKDILDVWLAPLKALRADQEPLQAIREYIRLKLEVSRQH
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
PQASRLFCLEMIQGAPLLKQELQGGLKALVDEKSAMIERWIAAGHIAAVEPHHLIFMLWA
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCEEEEEEEH
TTQHYADFWVQVEAVTGKTLDDEAFFQQTVENVQRVIIEGIRPR
HHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]