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The map label for this gene is trpI [H]

Identifier: 157370057

GI number: 157370057

Start: 1999297

End: 2000220

Strand: Direct

Name: trpI [H]

Synonym: Spro_1815

Alternate gene names: 157370057

Gene position: 1999297-2000220 (Clockwise)

Preceding gene: 157370048

Following gene: 157370059

Centisome position: 36.69

GC content: 57.79

Gene sequence:

>924_bases
ATGAAAACTGACGATATCGGTGAGAAAAACTCACGCAAGATAAACCGACCTCTGTCATTAGGGGGCTTGCGTTGTTTTGA
GGCTGCTGCACGGTTGGAAAGCTTTACCCAGGCGGCAAACAGCCTGAGCCTGACGCACGGCGCGGTTAGCCGAGCGGTCA
GGGCGCTGGAAGATGAACTTGGCGTTGCGTTGTTCGAACGTCGCCATCGGCGGGTATATTTGACCACCGCCGGGAGAGTG
CTGCTGCAAGCTTCACAACAGGCGTTTGGCATTCTGGAACATACGGCACAAACTCTGCGACAGCAGGCACAGGATGCACC
GCTGGTATTATCTTGCGAGCCGACGCTGTTAATGCGTTGGCTGATACCGCGTTTGCCGGCTTTTCAGCTGGCACATCCTG
ACATCAACCTGCAGTTGGTTGCGGGTGGTGGGCCGTTTTCGTTTAAAGACGGTATTAGCGCCGCTATCCGTCGTAACGAT
TTTGATTGGGGCGATAAGCTGCATAGCGTGACGCTGTTCGATGAGCGGGTTGGGCCGGTGTGCAAATCTGAAATGGTGCC
GCGTTTTATTGCCGTTAATGATGGCCAGCCCTCCTTGAACCATGACGCCGTATTGCTGCACTCAGCGACCCGGCCTGACG
CTTGGCGGCAGTGGGCACAGCAGCAGGGTGTGGCACTCAAGGGGCAACCGGAGCAGCGTTTCGATCACTTTTACTTCAGC
TTGCAGGCGGCGGTGGCGGGGGTAGGTGTCGCTATCGGTCCGTGGCAGCAGGTGCGTGATGACGTTGCCTCGGGGCTATT
GAGCGCTCCTTTTGGCTTCATGCCAGACGGCAGCGGTTACTGTTTGCTGACGCCGCAGGAAATCCGGCCGGGCAGTGCTC
TGGCCCGGCTGGCCGATTGGCTGAAACTCAGTGCCGATGGCTAG

Upstream 100 bases:

>100_bases
GGTAATGGTGACGACGGCGATGAGTTCGGTCATAGTAATCCTATAAAAAATCAGAAGAGAGTGTCTGATTTATAAAAGGA
ATGTTCGACGGTGACAAACG

Downstream 100 bases:

>100_bases
AAAGTAACGTTGCGGATAAAACGCAAGGGTTGCTCGCTGCGGTTGGCGTAGGCATAGTGCTGATCGCTGTTGAACACCTG
GGTTTGTCCGGCCTCCAGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: TrpBA operon transcriptional activator [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKTDDIGEKNSRKINRPLSLGGLRCFEAAARLESFTQAANSLSLTHGAVSRAVRALEDELGVALFERRHRRVYLTTAGRV
LLQASQQAFGILEHTAQTLRQQAQDAPLVLSCEPTLLMRWLIPRLPAFQLAHPDINLQLVAGGGPFSFKDGISAAIRRND
FDWGDKLHSVTLFDERVGPVCKSEMVPRFIAVNDGQPSLNHDAVLLHSATRPDAWRQWAQQQGVALKGQPEQRFDHFYFS
LQAAVAGVGVAIGPWQQVRDDVASGLLSAPFGFMPDGSGYCLLTPQEIRPGSALARLADWLKLSADG

Sequences:

>Translated_307_residues
MKTDDIGEKNSRKINRPLSLGGLRCFEAAARLESFTQAANSLSLTHGAVSRAVRALEDELGVALFERRHRRVYLTTAGRV
LLQASQQAFGILEHTAQTLRQQAQDAPLVLSCEPTLLMRWLIPRLPAFQLAHPDINLQLVAGGGPFSFKDGISAAIRRND
FDWGDKLHSVTLFDERVGPVCKSEMVPRFIAVNDGQPSLNHDAVLLHSATRPDAWRQWAQQQGVALKGQPEQRFDHFYFS
LQAAVAGVGVAIGPWQQVRDDVASGLLSAPFGFMPDGSGYCLLTPQEIRPGSALARLADWLKLSADG
>Mature_307_residues
MKTDDIGEKNSRKINRPLSLGGLRCFEAAARLESFTQAANSLSLTHGAVSRAVRALEDELGVALFERRHRRVYLTTAGRV
LLQASQQAFGILEHTAQTLRQQAQDAPLVLSCEPTLLMRWLIPRLPAFQLAHPDINLQLVAGGGPFSFKDGISAAIRRND
FDWGDKLHSVTLFDERVGPVCKSEMVPRFIAVNDGQPSLNHDAVLLHSATRPDAWRQWAQQQGVALKGQPEQRFDHFYFS
LQAAVAGVGVAIGPWQQVRDDVASGLLSAPFGFMPDGSGYCLLTPQEIRPGSALARLADWLKLSADG

Specific function: Activates the expression of the trpBA genes encoding the two tryptophan synthase subunits. In the absence of the inducer (indoleglycerol phosphate), trpI binds upstream of the trpAB operon, overlapping its own promoter region [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788706, Length=260, Percent_Identity=33.8461538461538, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789173, Length=255, Percent_Identity=32.156862745098, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786448, Length=296, Percent_Identity=29.7297297297297, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787589, Length=297, Percent_Identity=26.5993265993266, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI145693193, Length=138, Percent_Identity=34.0579710144928, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI145693105, Length=154, Percent_Identity=27.2727272727273, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787128, Length=307, Percent_Identity=24.1042345276873, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789639, Length=261, Percent_Identity=25.6704980842912, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33672; Mature: 33672

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTDDIGEKNSRKINRPLSLGGLRCFEAAARLESFTQAANSLSLTHGAVSRAVRALEDEL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVALFERRHRRVYLTTAGRVLLQASQQAFGILEHTAQTLRQQAQDAPLVLSCEPTLLMRW
HHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHH
LIPRLPAFQLAHPDINLQLVAGGGPFSFKDGISAAIRRNDFDWGDKLHSVTLFDERVGPV
HHHCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHEEEEEEHHHCCCH
CKSEMVPRFIAVNDGQPSLNHDAVLLHSATRPDAWRQWAQQQGVALKGQPEQRFDHFYFS
HHHHCCCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEECCCCHHHHHHHHHH
LQAAVAGVGVAIGPWQQVRDDVASGLLSAPFGFMPDGSGYCLLTPQEIRPGSALARLADW
HHHHHHHCCCEECCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHCCCHHHHHHHHHH
LKLSADG
HHHCCCC
>Mature Secondary Structure
MKTDDIGEKNSRKINRPLSLGGLRCFEAAARLESFTQAANSLSLTHGAVSRAVRALEDEL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVALFERRHRRVYLTTAGRVLLQASQQAFGILEHTAQTLRQQAQDAPLVLSCEPTLLMRW
HHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHH
LIPRLPAFQLAHPDINLQLVAGGGPFSFKDGISAAIRRNDFDWGDKLHSVTLFDERVGPV
HHHCCCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCHHHEEEEEEHHHCCCH
CKSEMVPRFIAVNDGQPSLNHDAVLLHSATRPDAWRQWAQQQGVALKGQPEQRFDHFYFS
HHHHCCCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCEECCCCHHHHHHHHHH
LQAAVAGVGVAIGPWQQVRDDVASGLLSAPFGFMPDGSGYCLLTPQEIRPGSALARLADW
HHHHHHHCCCEECCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHCCCHHHHHHHHHH
LKLSADG
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2492495; 2107533; 10984043 [H]