Click here to switch to the map view.

The map label for this gene is focA [H]

Identifier: 157369946

GI number: 157369946

Start: 1868319

End: 1869179

Strand: Reverse

Name: focA [H]

Synonym: Spro_1703

Alternate gene names: 157369946

Gene position: 1869179-1868319 (Counterclockwise)

Preceding gene: 157369947

Following gene: 157369945

Centisome position: 34.3

GC content: 54.01

Gene sequence:

>861_bases
GTGAAAGCTGACAACCCCTTCGATCTGATATTACCTGCAGCAACGGCGAAAATCGCTGAAGATGCAGGTGTGTATAAAGC
CACCAAGCATCCGCTGAAAACGTTTTATTTGGCGATCACTGCCGGCGTCTTTATTTCAATCGCATTCGTGTTTTATATCA
CTGCGACTACCGGCACTGCCGGTGTGCCTTTCGGCCTGGCAAAACTGGTCGGCGGCATCTGTTTCTCTCTGGGGTTAATG
CTGGTGGTGGTTTCGGGCGCCGACCTGTTCACCTCCACCGTGCTGATTGTCATTGCCAAGGCCAGCGGCCGCATCAGCTG
GTGCCAACTGGGCGCCAACTGGCTGAATGTCTATATTGGCAACCTGATTGGCGCACTGTTCTTTGTGGCACTCATCTGGT
TCTCTGGCGAGCACATGGTCGCCAACGGTCAGTGGGGCCTGAACGTCCTGCAAACCGCGGATCACAAGCTGCATCACACC
TTTGTTGAGGCCGTCTGCCTCGGCATCCTGGCTAACCTGATGGTCTGCCTGGCGGTGTGGATGAGCTACTCCGGCCGCAG
CCTGATGGACAAAATGTTCGCCATGATCCTGCCGGTCGGCATGTTTGTCGCCAGCGGTTTCGAACACAGCATCGCCAACA
TGTTTATGATCCCTATGGGTATCGTGGTTAAAAACTTCGCCACGCCAGAATTCTGGCAAGCCGTAGGGGCAACACCTGAG
CAGTTTGCTCATCTGACCGTAAGCAACTTTATCATCGACAACCTGATCCCGGTCACCATTGGCAACATCATTGGTGGCGG
CCTGCTGGTTGGGTTGACTTACTGGGTAATTTATCTGCGTGGTGGTGAACAACAGCACTAA

Upstream 100 bases:

>100_bases
TGCTTTAGTTTCTCGCCTTATGATCCAAATCAATTTTTACCCTATACTGCTTTGTTAGTATCTTATTGCGTTGTTTTAAC
CCTTAAAAGCGAGAGTTAGT

Downstream 100 bases:

>100_bases
GCTGTTAGTGACCGCGCGCCGAGTTAGAACTCCACAAATAAAGGTAGGTGTATAATGACCGAACTTAACGAGAAGTTAGC
CAAAGCTTGGGAAGGTTTCA

Product: formate transporter

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MKADNPFDLILPAATAKIAEDAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTAGVPFGLAKLVGGICFSLGLM
LVVVSGADLFTSTVLIVIAKASGRISWCQLGANWLNVYIGNLIGALFFVALIWFSGEHMVANGQWGLNVLQTADHKLHHT
FVEAVCLGILANLMVCLAVWMSYSGRSLMDKMFAMILPVGMFVASGFEHSIANMFMIPMGIVVKNFATPEFWQAVGATPE
QFAHLTVSNFIIDNLIPVTIGNIIGGGLLVGLTYWVIYLRGGEQQH

Sequences:

>Translated_286_residues
MKADNPFDLILPAATAKIAEDAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTAGVPFGLAKLVGGICFSLGLM
LVVVSGADLFTSTVLIVIAKASGRISWCQLGANWLNVYIGNLIGALFFVALIWFSGEHMVANGQWGLNVLQTADHKLHHT
FVEAVCLGILANLMVCLAVWMSYSGRSLMDKMFAMILPVGMFVASGFEHSIANMFMIPMGIVVKNFATPEFWQAVGATPE
QFAHLTVSNFIIDNLIPVTIGNIIGGGLLVGLTYWVIYLRGGEQQH
>Mature_286_residues
MKADNPFDLILPAATAKIAEDAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTAGVPFGLAKLVGGICFSLGLM
LVVVSGADLFTSTVLIVIAKASGRISWCQLGANWLNVYIGNLIGALFFVALIWFSGEHMVANGQWGLNVLQTADHKLHHT
FVEAVCLGILANLMVCLAVWMSYSGRSLMDKMFAMILPVGMFVASGFEHSIANMFMIPMGIVVKNFATPEFWQAVGATPE
QFAHLTVSNFIIDNLIPVTIGNIIGGGLLVGLTYWVIYLRGGEQQH

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=285, Percent_Identity=83.859649122807, Blast_Score=496, Evalue=1e-142,
Organism=Escherichia coli, GI1788837, Length=280, Percent_Identity=51.4285714285714, Blast_Score=278, Evalue=2e-76,
Organism=Escherichia coli, GI87082249, Length=259, Percent_Identity=29.7297297297297, Blast_Score=74, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6321779, Length=207, Percent_Identity=28.9855072463768, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 30814; Mature: 30814

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS01005 FORMATE_NITRITE_TP_1 ; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKADNPFDLILPAATAKIAEDAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTA
CCCCCCCCEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GVPFGLAKLVGGICFSLGLMLVVVSGADLFTSTVLIVIAKASGRISWCQLGANWLNVYIG
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHH
NLIGALFFVALIWFSGEHMVANGQWGLNVLQTADHKLHHTFVEAVCLGILANLMVCLAVW
HHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSYSGRSLMDKMFAMILPVGMFVASGFEHSIANMFMIPMGIVVKNFATPEFWQAVGATPE
HCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHH
QFAHLTVSNFIIDNLIPVTIGNIIGGGLLVGLTYWVIYLRGGEQQH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC
>Mature Secondary Structure
MKADNPFDLILPAATAKIAEDAGVYKATKHPLKTFYLAITAGVFISIAFVFYITATTGTA
CCCCCCCCEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GVPFGLAKLVGGICFSLGLMLVVVSGADLFTSTVLIVIAKASGRISWCQLGANWLNVYIG
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHH
NLIGALFFVALIWFSGEHMVANGQWGLNVLQTADHKLHHTFVEAVCLGILANLMVCLAVW
HHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSYSGRSLMDKMFAMILPVGMFVASGFEHSIANMFMIPMGIVVKNFATPEFWQAVGATPE
HCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCHH
QFAHLTVSNFIIDNLIPVTIGNIIGGGLLVGLTYWVIYLRGGEQQH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]