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The map label for this gene is artJ [H]

Identifier: 157369895

GI number: 157369895

Start: 1807485

End: 1808219

Strand: Reverse

Name: artJ [H]

Synonym: Spro_1652

Alternate gene names: 157369895

Gene position: 1808219-1807485 (Counterclockwise)

Preceding gene: 157369896

Following gene: 157369894

Centisome position: 33.19

GC content: 53.2

Gene sequence:

>735_bases
ATGAAAAAACTATTGCTGGCCGCCACGGTATTAGCAGGCATCGCCTTTAACGCCAGCGCAGCCGAGACCATTCGTTTCGC
CGCTTCCGCCACCTACCCGCCGTTTGAGTCGCTGGATGCCAACAACAAGATCGTGGGTTTTGATATCGATCTGGCTAACG
CGCTGTGCAAACAAATGAAGGCCGAATGTACCTTCACCAACCAGGCGTTCGACAGCCTGATCGCCGCGCTTAAATTCAAG
AAATACGACGCGGTGATCTCCGGTATGGACATCACGCCAGAGCGCAGCAAGCAAGTTGCCTTCACTCAGCCCTATTATGC
CAACTCGGCAATTGTGATTGCCGAAAAAGGCAAATTCAGTTCGCTGGCGGATCTGAAAGGGAAAAAGGTCGGCATGGAGA
ACGGTACCACCCACCAGAAATACATGCAGGACAAACACCCGGAGATTAACACCGTCTCTTACGACAGCTATCAGAACGCC
ATTCTCGAGTTGAAAAATGGCCGTATTGACGGCGTATTCGGTGATACCGCCGTGGTGAATGAGTGGCTGAAGAACAATCC
TCAGTTGGCTCCGGTCGGTGAACACGTTACCGATGCACAATACTTCGGTACCGGTCTGGGTATTGCGGTACGGCCGGACA
ATCAGGCGCTGCTGGCCAAGCTCAACAGTGCGCTGGACGCCATTAAGGCCGACGGCACCTACAAAGCCATTAACGACAAG
TGGTTCCCGCAGTAA

Upstream 100 bases:

>100_bases
TAACACCGACCGACAGCCGCCAAAACCTCTGGGTTATTGTCAGTAAAATCAAGCGGTGACGAGATAAAAACACGCAGTCT
TGCAGACAGGAGCTTTAAGC

Downstream 100 bases:

>100_bases
GCGCTATTCGGGGCGTGGAATGCCACGCCTTGTTTACCAAACCTAAGCATTGTCCACAGTTCCTTTCGGTGCTAAATTTG
ATACTAAATATAACACCAAA

Product: cationic amino acid ABC transporter periplasmic binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MKKLLLAATVLAGIAFNASAAETIRFAASATYPPFESLDANNKIVGFDIDLANALCKQMKAECTFTNQAFDSLIAALKFK
KYDAVISGMDITPERSKQVAFTQPYYANSAIVIAEKGKFSSLADLKGKKVGMENGTTHQKYMQDKHPEINTVSYDSYQNA
ILELKNGRIDGVFGDTAVVNEWLKNNPQLAPVGEHVTDAQYFGTGLGIAVRPDNQALLAKLNSALDAIKADGTYKAINDK
WFPQ

Sequences:

>Translated_244_residues
MKKLLLAATVLAGIAFNASAAETIRFAASATYPPFESLDANNKIVGFDIDLANALCKQMKAECTFTNQAFDSLIAALKFK
KYDAVISGMDITPERSKQVAFTQPYYANSAIVIAEKGKFSSLADLKGKKVGMENGTTHQKYMQDKHPEINTVSYDSYQNA
ILELKNGRIDGVFGDTAVVNEWLKNNPQLAPVGEHVTDAQYFGTGLGIAVRPDNQALLAKLNSALDAIKADGTYKAINDK
WFPQ
>Mature_244_residues
MKKLLLAATVLAGIAFNASAAETIRFAASATYPPFESLDANNKIVGFDIDLANALCKQMKAECTFTNQAFDSLIAALKFK
KYDAVISGMDITPERSKQVAFTQPYYANSAIVIAEKGKFSSLADLKGKKVGMENGTTHQKYMQDKHPEINTVSYDSYQNA
ILELKNGRIDGVFGDTAVVNEWLKNNPQLAPVGEHVTDAQYFGTGLGIAVRPDNQALLAKLNSALDAIKADGTYKAINDK
WFPQ

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=244, Percent_Identity=77.4590163934426, Blast_Score=399, Evalue=1e-113,
Organism=Escherichia coli, GI1787088, Length=242, Percent_Identity=67.7685950413223, Blast_Score=356, Evalue=1e-100,
Organism=Escherichia coli, GI1788649, Length=253, Percent_Identity=37.9446640316205, Blast_Score=170, Evalue=7e-44,
Organism=Escherichia coli, GI1788648, Length=228, Percent_Identity=37.719298245614, Blast_Score=152, Evalue=1e-38,
Organism=Escherichia coli, GI1788228, Length=252, Percent_Identity=29.3650793650794, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1787031, Length=244, Percent_Identity=31.9672131147541, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26567; Mature: 26567

Theoretical pI: Translated: 7.49; Mature: 7.49

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLLAATVLAGIAFNASAAETIRFAASATYPPFESLDANNKIVGFDIDLANALCKQMK
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHCCCCCCEEEEEHHHHHHHHHHHH
AECTFTNQAFDSLIAALKFKKYDAVISGMDITPERSKQVAFTQPYYANSAIVIAEKGKFS
HCCEECHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEECCCCCCCEEEEEECCCCC
SLADLKGKKVGMENGTTHQKYMQDKHPEINTVSYDSYQNAILELKNGRIDGVFGDTAVVN
HHHHCCCCEECCCCCCCHHHHHHHCCCCCCEEECCHHHHHHEEECCCEECEEECCHHHHH
EWLKNNPQLAPVGEHVTDAQYFGTGLGIAVRPDNQALLAKLNSALDAIKADGTYKAINDK
HHHHCCCCCCCCCCCCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCC
WFPQ
CCCC
>Mature Secondary Structure
MKKLLLAATVLAGIAFNASAAETIRFAASATYPPFESLDANNKIVGFDIDLANALCKQMK
CCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHCCCCCCEEEEEHHHHHHHHHHHH
AECTFTNQAFDSLIAALKFKKYDAVISGMDITPERSKQVAFTQPYYANSAIVIAEKGKFS
HCCEECHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEECCCCCCCEEEEEECCCCC
SLADLKGKKVGMENGTTHQKYMQDKHPEINTVSYDSYQNAILELKNGRIDGVFGDTAVVN
HHHHCCCCEECCCCCCCHHHHHHHCCCCCCEEECCHHHHHHEEECCCEECEEECCHHHHH
EWLKNNPQLAPVGEHVTDAQYFGTGLGIAVRPDNQALLAKLNSALDAIKADGTYKAINDK
HHHHCCCCCCCCCCCCCCHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCEEECCCC
WFPQ
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]