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The map label for this gene is rumB

Identifier: 157369893

GI number: 157369893

Start: 1805993

End: 1807120

Strand: Direct

Name: rumB

Synonym: Spro_1650

Alternate gene names: 157369893

Gene position: 1805993-1807120 (Clockwise)

Preceding gene: 157369892

Following gene: 157369901

Centisome position: 33.14

GC content: 57.62

Gene sequence:

>1128_bases
ATGCATTGCGCTTTGTATACGGCGGGCACCTGCCGTTCCTGTCAGTGGTTGGAAAAGCCGTACCCGCAGCAACTGACGGA
CAAACAGCATCATCTGCAATCACTGCTGAGCGACCGAGACGTTGCGCAGTGGCTAGAGCCGATCGCCGGCGAGCAGAGCG
CATTTCGCAATAAAGCCAAAATGGTAGTCAGCGGCAGCGTAGAGCGTCCGCTACTGGGCATGCTGCATCGTGACGGCACG
GCGGTGGATCTGAGCGCCTGTCCACTGTATCCCGCCAGTTTCTCGCCGATGTTTGATGTGCTGAAAAGTTTTATCGCCCG
CGCCGGCCTGACGCCCTACAACGTGGCGCGTAAACGTGGCGAACTGAAGTACCTGTTACTGACTGAAAGTACTCACAGTG
GTGGCGTAATGCTGCGCTTTGTACTTCGTTCAGACAACAAGCTGGCGCAACTGCGTGCCGCACTGCCGTGGCTGCAACAG
CAGTTGCCGCAGCTCAGGGTGATTTCCGCCAATATTCAGCCGGTACATATGGCGATTATGGAAGGGGAGCGCGAGATCCC
GCTGACCGAGCAACAGGCGCTGGAAGAGCAATTTAACCAGGTGCCGTTGTATATCCGCCCGCAAAGTTTCTTCCAGACCA
ATCCGCAGGTGGCCGCCGAGCTTTACGCCACCGCCCGTGACTGGGTGCGGGCACTGGCTATCGACAGTATGTGGGATCTG
TTCTGCGGTGTGGGTGGGTTTGGCCTGCATTGCGCGCTGCCGGAAACACGCCTGACCGGCATCGAAATTAGCGCCGAGGC
GATTGCCTGCGCGCGCCAATCGGCGAAAACTCTGGGTTTGCAACACGTCGATTTCCAGGCGCTGGATTCTACCCGGTTCG
CTACCGCCGAAGGCAGCGTGCCGCAACTGGTGCTGGTTAATCCGCCGCGACGCGGTATTGGCAAGGCACTGTGCGACTAT
CTGAACCAGATGGCACCGGGCTATATTCTGTATTCGAGCTGTAACGCCGAGAGCATGGCGAAAGACATTGAGATGTTGCC
GGATTACCGCATCGAGCGCGTCCAGCTGTTTGATATGTTCCCGCATACCGCCCATTACGAAGTACTGACGCTGCTGGTTC
GTAATTAA

Upstream 100 bases:

>100_bases
CCAGCCGCCGTTATTTCGCTGTGCGCAATTGAGATTTTAACGGCGTAGAGTGATAAACTCTGCGCCCTTAATTATTCACC
CCGAACTCAGGGTTATCTTC

Downstream 100 bases:

>100_bases
AGGTTATAAGTCATCCAGATTGACCTTAATACGTTTGCCACTCATCCGTGAGCGGACTATTTCAGCGAGTTCAATATCCT
CGAGGCATTCAGCAATGGCT

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MHCALYTAGTCRSCQWLEKPYPQQLTDKQHHLQSLLSDRDVAQWLEPIAGEQSAFRNKAKMVVSGSVERPLLGMLHRDGT
AVDLSACPLYPASFSPMFDVLKSFIARAGLTPYNVARKRGELKYLLLTESTHSGGVMLRFVLRSDNKLAQLRAALPWLQQ
QLPQLRVISANIQPVHMAIMEGEREIPLTEQQALEEQFNQVPLYIRPQSFFQTNPQVAAELYATARDWVRALAIDSMWDL
FCGVGGFGLHCALPETRLTGIEISAEAIACARQSAKTLGLQHVDFQALDSTRFATAEGSVPQLVLVNPPRRGIGKALCDY
LNQMAPGYILYSSCNAESMAKDIEMLPDYRIERVQLFDMFPHTAHYEVLTLLVRN

Sequences:

>Translated_375_residues
MHCALYTAGTCRSCQWLEKPYPQQLTDKQHHLQSLLSDRDVAQWLEPIAGEQSAFRNKAKMVVSGSVERPLLGMLHRDGT
AVDLSACPLYPASFSPMFDVLKSFIARAGLTPYNVARKRGELKYLLLTESTHSGGVMLRFVLRSDNKLAQLRAALPWLQQ
QLPQLRVISANIQPVHMAIMEGEREIPLTEQQALEEQFNQVPLYIRPQSFFQTNPQVAAELYATARDWVRALAIDSMWDL
FCGVGGFGLHCALPETRLTGIEISAEAIACARQSAKTLGLQHVDFQALDSTRFATAEGSVPQLVLVNPPRRGIGKALCDY
LNQMAPGYILYSSCNAESMAKDIEMLPDYRIERVQLFDMFPHTAHYEVLTLLVRN
>Mature_375_residues
MHCALYTAGTCRSCQWLEKPYPQQLTDKQHHLQSLLSDRDVAQWLEPIAGEQSAFRNKAKMVVSGSVERPLLGMLHRDGT
AVDLSACPLYPASFSPMFDVLKSFIARAGLTPYNVARKRGELKYLLLTESTHSGGVMLRFVLRSDNKLAQLRAALPWLQQ
QLPQLRVISANIQPVHMAIMEGEREIPLTEQQALEEQFNQVPLYIRPQSFFQTNPQVAAELYATARDWVRALAIDSMWDL
FCGVGGFGLHCALPETRLTGIEISAEAIACARQSAKTLGLQHVDFQALDSTRFATAEGSVPQLVLVNPPRRGIGKALCDY
LNQMAPGYILYSSCNAESMAKDIEMLPDYRIERVQLFDMFPHTAHYEVLTLLVRN

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily

Homologues:

Organism=Homo sapiens, GI34222389, Length=422, Percent_Identity=25.1184834123223, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI51173878, Length=422, Percent_Identity=25.1184834123223, Blast_Score=82, Evalue=8e-16,
Organism=Escherichia coli, GI1787083, Length=375, Percent_Identity=74.6666666666667, Blast_Score=594, Evalue=1e-171,
Organism=Escherichia coli, GI1789148, Length=375, Percent_Identity=26.4, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1790403, Length=184, Percent_Identity=28.2608695652174, Blast_Score=72, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24666579, Length=366, Percent_Identity=24.3169398907104, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMB_SERP5 (A8GCB4)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477882.1
- ProteinModelPortal:   A8GCB4
- SMR:   A8GCB4
- STRING:   A8GCB4
- GeneID:   5604092
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1650
- eggNOG:   COG2265
- HOGENOM:   HBG296593
- OMA:   QPQHAAI
- ProtClustDB:   PRK03522
- BioCyc:   SPRO399741:SPRO_1650-MONOMER
- HAMAP:   MF_01012
- InterPro:   IPR011825
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02085

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.189

Molecular weight: Translated: 42036; Mature: 42036

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 334-334 BINDING 212-212 BINDING 241-241 BINDING 262-262 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHCALYTAGTCRSCQWLEKPYPQQLTDKQHHLQSLLSDRDVAQWLEPIAGEQSAFRNKAK
CEEEEEECCCCCCCHHHCCCCCHHHCCHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHE
MVVSGSVERPLLGMLHRDGTAVDLSACPLYPASFSPMFDVLKSFIARAGLTPYNVARKRG
EEEECCCCCHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCC
ELKYLLLTESTHSGGVMLRFVLRSDNKLAQLRAALPWLQQQLPQLRVISANIQPVHMAIM
CEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEHEE
EGEREIPLTEQQALEEQFNQVPLYIRPQSFFQTNPQVAAELYATARDWVRALAIDSMWDL
CCCCCCCCCHHHHHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FCGVGGFGLHCALPETRLTGIEISAEAIACARQSAKTLGLQHVDFQALDSTRFATAEGSV
HHCCCCCCEEEECCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEECCCCC
PQLVLVNPPRRGIGKALCDYLNQMAPGYILYSSCNAESMAKDIEMLPDYRIERVQLFDMF
CEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHC
PHTAHYEVLTLLVRN
CCCHHHHHHHHHHCC
>Mature Secondary Structure
MHCALYTAGTCRSCQWLEKPYPQQLTDKQHHLQSLLSDRDVAQWLEPIAGEQSAFRNKAK
CEEEEEECCCCCCCHHHCCCCCHHHCCHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHE
MVVSGSVERPLLGMLHRDGTAVDLSACPLYPASFSPMFDVLKSFIARAGLTPYNVARKRG
EEEECCCCCHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCC
ELKYLLLTESTHSGGVMLRFVLRSDNKLAQLRAALPWLQQQLPQLRVISANIQPVHMAIM
CEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEHEE
EGEREIPLTEQQALEEQFNQVPLYIRPQSFFQTNPQVAAELYATARDWVRALAIDSMWDL
CCCCCCCCCHHHHHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FCGVGGFGLHCALPETRLTGIEISAEAIACARQSAKTLGLQHVDFQALDSTRFATAEGSV
HHCCCCCCEEEECCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEECCCCC
PQLVLVNPPRRGIGKALCDYLNQMAPGYILYSSCNAESMAKDIEMLPDYRIERVQLFDMF
CEEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHHC
PHTAHYEVLTLLVRN
CCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA