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The map label for this gene is potH [H]

Identifier: 157369890

GI number: 157369890

Start: 1803441

End: 1804406

Strand: Direct

Name: potH [H]

Synonym: Spro_1647

Alternate gene names: 157369890

Gene position: 1803441-1804406 (Clockwise)

Preceding gene: 157369889

Following gene: 157369891

Centisome position: 33.1

GC content: 56.42

Gene sequence:

>966_bases
ATGACAATGCTTGCTGAACACACACCGCCGGAACCACCGGCCAAAGGGCCCGGCCCGTTAAAAGCCTTTTTCCAGCGCCT
GCAGATGGCCCATGGCCGCAAGCTGGTGATCGCGGTGCCGCTGCTGTGGTTGACGCTGCTGTTCCTGCTGCCGTTCCTGA
TCGTGTTCAAGATCAGCCTGGCGGAACTGGCGTTGGCGGTCCCGCCCTACACTGACCTGATGAGCTGGGCAGAGGGTAAG
CTGGACATCGCGCTCAATTTTGCCAACTACCTGCAACTGACCGACGATCCGCTGTATATAGACGCTTATCTGCAATCGCT
GCAGGTGGCGGCGGTATCGACGGTTTGCTGTCTGATCATCGGCTATCCGCTGGCCTGGGCGGTGGCGCACAGCAAATCCT
CAACCCGCAATATTTTGCTGTTGTTGGTGATCCTGCCGTCGTGGACCTCGTTCCTGATCCGCGTTTACGCCTGGATGGGC
ATTCTGAAAAATAACGGTATCCTCAATAACTTCTTGTTATGGACCGGGGTGATCGATCAGCCGTTGGTGATCCTGCACAC
CAATCTGGCGGTGTATATCGGCGTGGTGTACTCCTACCTGCCGTTTATGGTGCTGCCGATTTATACCGCGCTGACGCGGT
TGGACTATTCATTGGTGGAGGCGTCGCTGGATCTGGGGGCTAAACCGTTGAAAACCTTTTTCAGCGTGATTATGCCGCTG
ACCCGCGGCGGGATTATTGCCGGTTCTATGCTGGTGTTTATCCCGGCGGTCGGCGAATTTGTGATCCCGGAACTGCTGGG
TGGGCCGGACAGTATTATGATCGGCAGGGTACTGTGGCAGGAGTTCTTTAATAACCGTGACTGGCCAGTAGCCTCGGCAG
TGGCCACCGTCATGCTGCTGCTGTTGATCGTACCGATTCTCTGGTTCCACAAGCACCAGAACAAGGAAATAGGGGGGCAG
GGATGA

Upstream 100 bases:

>100_bases
AAAGGGATGCCGACCTGGGGCGACGAAGTGCGCCTGTGCTGGGAAGCCGACAGCTGTGTCGTGTTGACGGTGTGAGTCGA
GAAGGTGAGGAATAATCATT

Downstream 100 bases:

>100_bases
ACAACTTGCCGGTAGTGCGCTCGCCGTGGCGTATCGCTATTTTGGTAGTGGGTTTCACCTTCTTGTATGCGCCGATGCTG
ATGCTGGTGATCTACTCGTT

Product: putrescine ABC transporter membrane protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTMLAEHTPPEPPAKGPGPLKAFFQRLQMAHGRKLVIAVPLLWLTLLFLLPFLIVFKISLAELALAVPPYTDLMSWAEGK
LDIALNFANYLQLTDDPLYIDAYLQSLQVAAVSTVCCLIIGYPLAWAVAHSKSSTRNILLLLVILPSWTSFLIRVYAWMG
ILKNNGILNNFLLWTGVIDQPLVILHTNLAVYIGVVYSYLPFMVLPIYTALTRLDYSLVEASLDLGAKPLKTFFSVIMPL
TRGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFFNNRDWPVASAVATVMLLLLIVPILWFHKHQNKEIGGQ
G

Sequences:

>Translated_321_residues
MTMLAEHTPPEPPAKGPGPLKAFFQRLQMAHGRKLVIAVPLLWLTLLFLLPFLIVFKISLAELALAVPPYTDLMSWAEGK
LDIALNFANYLQLTDDPLYIDAYLQSLQVAAVSTVCCLIIGYPLAWAVAHSKSSTRNILLLLVILPSWTSFLIRVYAWMG
ILKNNGILNNFLLWTGVIDQPLVILHTNLAVYIGVVYSYLPFMVLPIYTALTRLDYSLVEASLDLGAKPLKTFFSVIMPL
TRGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFFNNRDWPVASAVATVMLLLLIVPILWFHKHQNKEIGGQ
G
>Mature_320_residues
TMLAEHTPPEPPAKGPGPLKAFFQRLQMAHGRKLVIAVPLLWLTLLFLLPFLIVFKISLAELALAVPPYTDLMSWAEGKL
DIALNFANYLQLTDDPLYIDAYLQSLQVAAVSTVCCLIIGYPLAWAVAHSKSSTRNILLLLVILPSWTSFLIRVYAWMGI
LKNNGILNNFLLWTGVIDQPLVILHTNLAVYIGVVYSYLPFMVLPIYTALTRLDYSLVEASLDLGAKPLKTFFSVIMPLT
RGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFFNNRDWPVASAVATVMLLLLIVPILWFHKHQNKEIGGQG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=312, Percent_Identity=82.3717948717949, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1787369, Length=210, Percent_Identity=47.6190476190476, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1787713, Length=219, Percent_Identity=33.3333333333333, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1788764, Length=221, Percent_Identity=25.7918552036199, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35626; Mature: 35495

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLAEHTPPEPPAKGPGPLKAFFQRLQMAHGRKLVIAVPLLWLTLLFLLPFLIVFKISL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
AELALAVPPYTDLMSWAEGKLDIALNFANYLQLTDDPLYIDAYLQSLQVAAVSTVCCLII
HHHHHCCCCHHHHHHHCCCCEEEEEEHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHH
GYPLAWAVAHSKSSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGILNNFLLWTGVIDQ
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
PLVILHTNLAVYIGVVYSYLPFMVLPIYTALTRLDYSLVEASLDLGAKPLKTFFSVIMPL
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
TRGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFFNNRDWPVASAVATVMLL
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LLIVPILWFHKHQNKEIGGQG
HHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TMLAEHTPPEPPAKGPGPLKAFFQRLQMAHGRKLVIAVPLLWLTLLFLLPFLIVFKISL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
AELALAVPPYTDLMSWAEGKLDIALNFANYLQLTDDPLYIDAYLQSLQVAAVSTVCCLII
HHHHHCCCCHHHHHHHCCCCEEEEEEHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHH
GYPLAWAVAHSKSSTRNILLLLVILPSWTSFLIRVYAWMGILKNNGILNNFLLWTGVIDQ
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
PLVILHTNLAVYIGVVYSYLPFMVLPIYTALTRLDYSLVEASLDLGAKPLKTFFSVIMPL
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
TRGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQEFFNNRDWPVASAVATVMLL
HCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LLIVPILWFHKHQNKEIGGQG
HHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]