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The map label for this gene is wcaJ [H]

Identifier: 157369837

GI number: 157369837

Start: 1741492

End: 1742886

Strand: Direct

Name: wcaJ [H]

Synonym: Spro_1594

Alternate gene names: 157369837

Gene position: 1741492-1742886 (Clockwise)

Preceding gene: 157369836

Following gene: 157369838

Centisome position: 31.96

GC content: 36.85

Gene sequence:

>1395_bases
ATGGCAATCTTACATCATAGAACCCATTCAAAAACCAATGCATCATTAATTTCTATGGCGCAAAGATTTTCTGATATATT
TATTATTTTTCTTGGTCTGTATGTTATTGTTTTTTTAAATTCCCAGCCTTTTAATTATAGGCACTTATTGGTATTTTTAA
TAGTTCTGGTCGTTTATCAGATGATTGGAGGGATGACTGATTTTTATCGGTCATGGCGTGGCGGTAAAATATCAACGGAA
CTTATTTTAATTATACAAAATTGGACGCTGAGTTTTATTTTTACAATAGGCTTAGTTTCGTTTCTACCCGAGTTTGATTT
TTCTCTCAAAATCTTCTTTCAATGGTATCTTTTAGTGAGCCTTGGTCTCGTCGGGTGTCGTATCGCGATACGATTTATCG
CAGGCTTCGTTCGTAACCTTGGTTATAATACAAGGAGAGTTGCAGTTGCTGGTTCAATGCCTGTTGGTCTAAGCTTACTC
CGTGGTTTTAAAGCTGACTCTTGGCTAGGTTTTATGGTTGTTGGTGTTTATGACGATACCCCTATTTCGGAAGAGGGCAT
TGAGTATGTTGGGGATTTTTCAAAATTAATCGAAGATGCACGCGAAGGAAGACTTGATAGAATTTATATTGCTATGAGTA
TGGGGGATGAGGTTAATATTAAAAACTTAGTGAAAAAGCTGACGGATACAACGTGTTCGGTCATGCTTATACCTGATTTG
TTTACTTTTAATATTTTACAGTCTCGAACGGAAGAAGTTAATGGAGTACCAGTAGTTCCATTGTTCGATACTCATCTGAA
TGGTATTAACAAGGTTTTCAAACGTTTGGAAGATATTACTCTTTCGATGATGATATTAGCCTTTATTTCACCTGTGCTAA
TTTTGATCTCTTTATTTGTTAAGTTTACATCACCTGGCCCAGTGATATTTAGACAGACACGTTACGGCATGGACGGAAAA
CCTATCAAAGTTTGGAAGTTTCGTTCAATGAGTGTTATGGAAAATGATAATACTGTTGTACAGGCAAAAAAACACGATGC
TAGAATTACTAAAGTTGGGAAATTTTTGCGGAGAACGTCCTTGGATGAGCTTCCTCAATTTTTCAATGTTTTATTTGGTG
AAATGTCTATTGTAGGTCCACGACCACATGCCGTTTCTCATAACGAGCAATACCGTAGTCTGATTGAAGGCTATATGCTT
AGACATAAAGTTAAGCCTGGGATAACGGGTTGGGCACAAATAAATGGTTGGAGAGGGGAGACCGACACACTAGACAAAAT
GCAGAAGCGTGTAGAATACGATCTTGAATACATTCGTGAATGGAGTGTTTGGTTCGATATAAAAATAATTATCCTTACTG
TTTTTAAGGGGTTTATGCATAAGTCTGCATACTGA

Upstream 100 bases:

>100_bases
TGTAGAGTCAATTTTTTCAACTCCATCATCATCTTTATTTCGGGGATAGAAAACGGTGCTTTCGGTTGGTTATACGTCTA
TTCTAAATTAGGTAATTTTA

Downstream 100 bases:

>100_bases
TTCTTTCTTAAAGATAAAATTTTTAGCCTTGGAAGTGAAATTCCAAGGCTTTTTTTTTGCATTTAAGTAGCGTACTACTT
AAGTGATGTGAAACGATTTC

Product: putative UDP-glucose lipid carrier transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MAILHHRTHSKTNASLISMAQRFSDIFIIFLGLYVIVFLNSQPFNYRHLLVFLIVLVVYQMIGGMTDFYRSWRGGKISTE
LILIIQNWTLSFIFTIGLVSFLPEFDFSLKIFFQWYLLVSLGLVGCRIAIRFIAGFVRNLGYNTRRVAVAGSMPVGLSLL
RGFKADSWLGFMVVGVYDDTPISEEGIEYVGDFSKLIEDAREGRLDRIYIAMSMGDEVNIKNLVKKLTDTTCSVMLIPDL
FTFNILQSRTEEVNGVPVVPLFDTHLNGINKVFKRLEDITLSMMILAFISPVLILISLFVKFTSPGPVIFRQTRYGMDGK
PIKVWKFRSMSVMENDNTVVQAKKHDARITKVGKFLRRTSLDELPQFFNVLFGEMSIVGPRPHAVSHNEQYRSLIEGYML
RHKVKPGITGWAQINGWRGETDTLDKMQKRVEYDLEYIREWSVWFDIKIIILTVFKGFMHKSAY

Sequences:

>Translated_464_residues
MAILHHRTHSKTNASLISMAQRFSDIFIIFLGLYVIVFLNSQPFNYRHLLVFLIVLVVYQMIGGMTDFYRSWRGGKISTE
LILIIQNWTLSFIFTIGLVSFLPEFDFSLKIFFQWYLLVSLGLVGCRIAIRFIAGFVRNLGYNTRRVAVAGSMPVGLSLL
RGFKADSWLGFMVVGVYDDTPISEEGIEYVGDFSKLIEDAREGRLDRIYIAMSMGDEVNIKNLVKKLTDTTCSVMLIPDL
FTFNILQSRTEEVNGVPVVPLFDTHLNGINKVFKRLEDITLSMMILAFISPVLILISLFVKFTSPGPVIFRQTRYGMDGK
PIKVWKFRSMSVMENDNTVVQAKKHDARITKVGKFLRRTSLDELPQFFNVLFGEMSIVGPRPHAVSHNEQYRSLIEGYML
RHKVKPGITGWAQINGWRGETDTLDKMQKRVEYDLEYIREWSVWFDIKIIILTVFKGFMHKSAY
>Mature_463_residues
AILHHRTHSKTNASLISMAQRFSDIFIIFLGLYVIVFLNSQPFNYRHLLVFLIVLVVYQMIGGMTDFYRSWRGGKISTEL
ILIIQNWTLSFIFTIGLVSFLPEFDFSLKIFFQWYLLVSLGLVGCRIAIRFIAGFVRNLGYNTRRVAVAGSMPVGLSLLR
GFKADSWLGFMVVGVYDDTPISEEGIEYVGDFSKLIEDAREGRLDRIYIAMSMGDEVNIKNLVKKLTDTTCSVMLIPDLF
TFNILQSRTEEVNGVPVVPLFDTHLNGINKVFKRLEDITLSMMILAFISPVLILISLFVKFTSPGPVIFRQTRYGMDGKP
IKVWKFRSMSVMENDNTVVQAKKHDARITKVGKFLRRTSLDELPQFFNVLFGEMSIVGPRPHAVSHNEQYRSLIEGYMLR
HKVKPGITGWAQINGWRGETDTLDKMQKRVEYDLEYIREWSVWFDIKIIILTVFKGFMHKSAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=464, Percent_Identity=66.1637931034483, Blast_Score=645, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 53381; Mature: 53249

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILHHRTHSKTNASLISMAQRFSDIFIIFLGLYVIVFLNSQPFNYRHLLVFLIVLVVYQ
CCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
MIGGMTDFYRSWRGGKISTELILIIQNWTLSFIFTIGLVSFLPEFDFSLKIFFQWYLLVS
HHCCHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LGLVGCRIAIRFIAGFVRNLGYNTRRVAVAGSMPVGLSLLRGFKADSWLGFMVVGVYDDT
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCCEEEEEEEEEECCC
PISEEGIEYVGDFSKLIEDAREGRLDRIYIAMSMGDEVNIKNLVKKLTDTTCSVMLIPDL
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCH
FTFNILQSRTEEVNGVPVVPLFDTHLNGINKVFKRLEDITLSMMILAFISPVLILISLFV
HHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFTSPGPVIFRQTRYGMDGKPIKVWKFRSMSVMENDNTVVQAKKHDARITKVGKFLRRTS
HHCCCCCEEEEECCCCCCCCCEEEEEECCCEEEECCCCEEEECHHHHHHHHHHHHHHHCC
LDELPQFFNVLFGEMSIVGPRPHAVSHNEQYRSLIEGYMLRHKVKPGITGWAQINGWRGE
HHHHHHHHHHHHHCHHEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
TDTLDKMQKRVEYDLEYIREWSVWFDIKIIILTVFKGFMHKSAY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AILHHRTHSKTNASLISMAQRFSDIFIIFLGLYVIVFLNSQPFNYRHLLVFLIVLVVYQ
CEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
MIGGMTDFYRSWRGGKISTELILIIQNWTLSFIFTIGLVSFLPEFDFSLKIFFQWYLLVS
HHCCHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LGLVGCRIAIRFIAGFVRNLGYNTRRVAVAGSMPVGLSLLRGFKADSWLGFMVVGVYDDT
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCCCCEEEEEEEEEECCC
PISEEGIEYVGDFSKLIEDAREGRLDRIYIAMSMGDEVNIKNLVKKLTDTTCSVMLIPDL
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCH
FTFNILQSRTEEVNGVPVVPLFDTHLNGINKVFKRLEDITLSMMILAFISPVLILISLFV
HHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KFTSPGPVIFRQTRYGMDGKPIKVWKFRSMSVMENDNTVVQAKKHDARITKVGKFLRRTS
HHCCCCCEEEEECCCCCCCCCEEEEEECCCEEEECCCCEEEECHHHHHHHHHHHHHHHCC
LDELPQFFNVLFGEMSIVGPRPHAVSHNEQYRSLIEGYMLRHKVKPGITGWAQINGWRGE
HHHHHHHHHHHHHCHHEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCC
TDTLDKMQKRVEYDLEYIREWSVWFDIKIIILTVFKGFMHKSAY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]