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The map label for this gene is actII-2 [H]

Identifier: 157369708

GI number: 157369708

Start: 1596961

End: 1598382

Strand: Direct

Name: actII-2 [H]

Synonym: Spro_1465

Alternate gene names: 157369708

Gene position: 1596961-1598382 (Clockwise)

Preceding gene: 157369706

Following gene: 157369709

Centisome position: 29.31

GC content: 62.1

Gene sequence:

>1422_bases
ATGGAACAGTCATTAGCGGGAGACGAGCGTCGTCTGTCGGGGTTGCCGGTGTTGTTGCTGGGCGGTTTTGTCACGGTGTT
CGACCTGTTTGTGGTGAACGTGGCAGCGCCGGTGATCCAGCAACAATTCGCCGCTGATTTTGCCGGCCTGGGTCTGATTA
TTGCCGGTTATGAGCTGGCATTCGGCGTGTTGCTGATTGCCGGTGGGCGGTTGGGCGATCGCTTTGGTCGTCGTCGCATG
TTCAGTCTCGGCATGTTGGGCTTTACCTTAACGTCTGCGCTGTGTGGCTTGGCGACCTCTCTGAGCATGTTGATCGTAGC
GCGCTTTTTGCAGGGGGCGACGGCGGCGCTGTTGTTCCCGCAGATCTATGCGTTGCTGCGCGTGTTGTATGGCCCGCAGC
AGCGGCGGCGGGCCTTTGCCTGGCTGGGTATGACGCTGGGGCTGGCGGCGATTTTGGGTCAGATTTTCGGTGGCTTTATT
ATCGAAGCCGATATCTTCGGCAGCGGTTGGCGAATGATTTTTCTGATCAATCTGCCGGTGGGAGCGCTGGCCATGCTGGC
GGCGCGGCGTATTCCCGAATCACGCCTCGAACAGGCGCAGCGGCTGGATGGCGTTGGCCTGCTGTTGGCGGCAACCGGGC
TGTTGTTGCTGCTGGTTCCGCTGCTGGAAGGGCCGTCGCGTGGCTGGCCCTGGTGGGTGTTGCCTACGCTGGCGCTGGGC
ATAGCGGTACTGTGGGCCTTTATCCGCTGGGAACGGCGGTTGGCGCAGCGTGCGGGGGACGCGGTGCTGGATCCGGCATT
GCTGCGGCAGGGGAGTTTTACCCCGGGCGTGGCAGTGGTGCTGGCGATTTACTCGACCGCCTCTTCGTTCTTTTTGTGTT
TTGCCCTGCTGTTGCAGGCGGGGATTGGCCTGACACCGTTTCAGGCCGGCAGCCTGTTTGCACCCGCCAGCGTGGCTTTT
ATGCTGGCCTCATTGCTGGCACCGACTCTGGTACAGCGCTGGGGCAACGGCGTGCTGGCGCTTGGCGTGGTGATTTACGC
CACCGGGATGGCACTGCTGGTGGCGCAGGTGCTGTGGGGCGCGGCGCTGGTTCAGCCACTGTGGCTGTTGCCGGGGCTGG
TTGTTCTGGGTTTTGGTCAGGCACTGAGTATGACACCGCTGCTTAACCTGGTGCTCGGACTGGCAAAAGAACAACAGGCG
GGGATGGCGGCCGGGGTGATTTCCACCGTTCAACAGGTGGGTGGGGCGTTGGGGATTGCCGCCAGCGGCGCGTTTTTTGT
TCCCTTGTTGGCGGGCGGCGCGGGCGCAGAGGGTTATGGGCGGGCGTTTGCCGGGGCCATGGTTTACAACCTGCTGGCGA
TGGCCGTTGCCTTGGTGCTGCTGAGGTGGATCATCCAACAACAGCGCAAAGTGGATCTTTAG

Upstream 100 bases:

>100_bases
AGTGGCTGTCCTGTTGCTGTTAGTAATAGTATAACTGGCTATATAGTAACAGTTTAATACATGTTCCATACTGCCGCCAT
CCCAATCTCTGGAGGTCATT

Downstream 100 bases:

>100_bases
TAAAGCTGTGGCGGGTGTTATTGCTATTCATCCACCCGATAACGTTGAAGGATGCCTGACGTCATGTACCAACCCGCCTC
ATTTCGCGATGACCGCCTGG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MEQSLAGDERRLSGLPVLLLGGFVTVFDLFVVNVAAPVIQQQFAADFAGLGLIIAGYELAFGVLLIAGGRLGDRFGRRRM
FSLGMLGFTLTSALCGLATSLSMLIVARFLQGATAALLFPQIYALLRVLYGPQQRRRAFAWLGMTLGLAAILGQIFGGFI
IEADIFGSGWRMIFLINLPVGALAMLAARRIPESRLEQAQRLDGVGLLLAATGLLLLLVPLLEGPSRGWPWWVLPTLALG
IAVLWAFIRWERRLAQRAGDAVLDPALLRQGSFTPGVAVVLAIYSTASSFFLCFALLLQAGIGLTPFQAGSLFAPASVAF
MLASLLAPTLVQRWGNGVLALGVVIYATGMALLVAQVLWGAALVQPLWLLPGLVVLGFGQALSMTPLLNLVLGLAKEQQA
GMAAGVISTVQQVGGALGIAASGAFFVPLLAGGAGAEGYGRAFAGAMVYNLLAMAVALVLLRWIIQQQRKVDL

Sequences:

>Translated_473_residues
MEQSLAGDERRLSGLPVLLLGGFVTVFDLFVVNVAAPVIQQQFAADFAGLGLIIAGYELAFGVLLIAGGRLGDRFGRRRM
FSLGMLGFTLTSALCGLATSLSMLIVARFLQGATAALLFPQIYALLRVLYGPQQRRRAFAWLGMTLGLAAILGQIFGGFI
IEADIFGSGWRMIFLINLPVGALAMLAARRIPESRLEQAQRLDGVGLLLAATGLLLLLVPLLEGPSRGWPWWVLPTLALG
IAVLWAFIRWERRLAQRAGDAVLDPALLRQGSFTPGVAVVLAIYSTASSFFLCFALLLQAGIGLTPFQAGSLFAPASVAF
MLASLLAPTLVQRWGNGVLALGVVIYATGMALLVAQVLWGAALVQPLWLLPGLVVLGFGQALSMTPLLNLVLGLAKEQQA
GMAAGVISTVQQVGGALGIAASGAFFVPLLAGGAGAEGYGRAFAGAMVYNLLAMAVALVLLRWIIQQQRKVDL
>Mature_473_residues
MEQSLAGDERRLSGLPVLLLGGFVTVFDLFVVNVAAPVIQQQFAADFAGLGLIIAGYELAFGVLLIAGGRLGDRFGRRRM
FSLGMLGFTLTSALCGLATSLSMLIVARFLQGATAALLFPQIYALLRVLYGPQQRRRAFAWLGMTLGLAAILGQIFGGFI
IEADIFGSGWRMIFLINLPVGALAMLAARRIPESRLEQAQRLDGVGLLLAATGLLLLLVPLLEGPSRGWPWWVLPTLALG
IAVLWAFIRWERRLAQRAGDAVLDPALLRQGSFTPGVAVVLAIYSTASSFFLCFALLLQAGIGLTPFQAGSLFAPASVAF
MLASLLAPTLVQRWGNGVLALGVVIYATGMALLVAQVLWGAALVQPLWLLPGLVVLGFGQALSMTPLLNLVLGLAKEQQA
GMAAGVISTVQQVGGALGIAASGAFFVPLLAGGAGAEGYGRAFAGAMVYNLLAMAVALVLLRWIIQQQRKVDL

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=473, Percent_Identity=26.4270613107822, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI87081983, Length=437, Percent_Identity=27.9176201372998, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788710, Length=417, Percent_Identity=26.8585131894484, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789042, Length=305, Percent_Identity=28.5245901639344, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788392, Length=406, Percent_Identity=26.3546798029557, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322958, Length=313, Percent_Identity=24.2811501597444, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324954, Length=426, Percent_Identity=23.4741784037559, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49912; Mature: 49912

Theoretical pI: Translated: 10.72; Mature: 10.72

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQSLAGDERRLSGLPVLLLGGFVTVFDLFVVNVAAPVIQQQFAADFAGLGLIIAGYELA
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGVLLIAGGRLGDRFGRRRMFSLGMLGFTLTSALCGLATSLSMLIVARFLQGATAALLFP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
QIYALLRVLYGPQQRRRAFAWLGMTLGLAAILGQIFGGFIIEADIFGSGWRMIFLINLPV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEECCH
GALAMLAARRIPESRLEQAQRLDGVGLLLAATGLLLLLVPLLEGPSRGWPWWVLPTLALG
HHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
IAVLWAFIRWERRLAQRAGDAVLDPALLRQGSFTPGVAVVLAIYSTASSFFLCFALLLQA
HHHHHHHHHHHHHHHHHCCCHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GIGLTPFQAGSLFAPASVAFMLASLLAPTLVQRWGNGVLALGVVIYATGMALLVAQVLWG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AALVQPLWLLPGLVVLGFGQALSMTPLLNLVLGLAKEQQAGMAAGVISTVQQVGGALGIA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ASGAFFVPLLAGGAGAEGYGRAFAGAMVYNLLAMAVALVLLRWIIQQQRKVDL
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEQSLAGDERRLSGLPVLLLGGFVTVFDLFVVNVAAPVIQQQFAADFAGLGLIIAGYELA
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGVLLIAGGRLGDRFGRRRMFSLGMLGFTLTSALCGLATSLSMLIVARFLQGATAALLFP
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
QIYALLRVLYGPQQRRRAFAWLGMTLGLAAILGQIFGGFIIEADIFGSGWRMIFLINLPV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEECCH
GALAMLAARRIPESRLEQAQRLDGVGLLLAATGLLLLLVPLLEGPSRGWPWWVLPTLALG
HHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
IAVLWAFIRWERRLAQRAGDAVLDPALLRQGSFTPGVAVVLAIYSTASSFFLCFALLLQA
HHHHHHHHHHHHHHHHHCCCHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GIGLTPFQAGSLFAPASVAFMLASLLAPTLVQRWGNGVLALGVVIYATGMALLVAQVLWG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AALVQPLWLLPGLVVLGFGQALSMTPLLNLVLGLAKEQQAGMAAGVISTVQQVGGALGIA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ASGAFFVPLLAGGAGAEGYGRAFAGAMVYNLLAMAVALVLLRWIIQQQRKVDL
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]