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The map label for this gene is sucB [H]

Identifier: 157369511

GI number: 157369511

Start: 1395290

End: 1396504

Strand: Direct

Name: sucB [H]

Synonym: Spro_1268

Alternate gene names: 157369511

Gene position: 1395290-1396504 (Clockwise)

Preceding gene: 157369510

Following gene: 157369512

Centisome position: 25.61

GC content: 57.12

Gene sequence:

>1215_bases
ATGAGTAGCGTAGATATTCTGGTTCCTGACCTTCCTGAATCGGTTGCCGATGCGACCGTCGCCACCTGGCACAAGAAACC
AGGTGACAGCGTCCAGCGTGACGAAGTTCTGGTTGAAATCGAAACTGACAAAGTGGTGCTGGAAGTTCCGGCCAGCGAAG
CAGGCATTCTGGATGCGATCGTGGAAGAAGAGGGCGCAACCGTGCTTTCTCGCCAGATCCTTGGCCGTATTCGTCCGGGC
GACAGCTCTGGCAAGCCGACCGAAGAAAAAAGCCAGGCTAAAGAAGCCACTCCAGCCCAGCGCGCAACCGCCAGCCTGGA
AGAAGAGAGCAACGACGCACTCAGCCCGGCTATCCGCCGCCTGATTGCCGAGCATGACCTCGATGCAGCTGCCATCAAAG
GCAGCGGCGTGGGTGGCCGTATCACCCGCGAAGACGTGGAAGCGCACCTGGCCAACGGCAAGAAAGCCGACAAGCCGGCC
GCCGTTGAAGCCGCTCCGCAGCCTGCTCTGAGCGGTCGCAGCGAGAAGCGTGTTCCTATGACCCGTCTGCGCAAGCGCGT
GGCCGAGCGCCTGTTGGAAGCGAAGAACAGCACTGCGATGCTGACGACCTTCAACGAAATCAACATGCAGCCGATCATGG
ACATGCGCAAGCAGTACGGTGAAGCCTTCGAAAAACGCCACGGTGTACGTCTGGGCTTCATGTCCTTCTACATCAAGGCG
GTAGTTGAAGCGCTGAAACGCTTCCCGGAAGTGAACGCTTCTATCGACGGCACCGACGTGGTGTACCACAACTATTTCGA
TATCAGCATTGCGGTATCTACTCCTCGTGGCCTGGTGACCCCGGTGCTGCGCGATGTAGACAGCATGAGCATGGCGGACA
TCGAGAAGAAAATCAAAGAGCTGGCAGTCAAAGGCCGTGATGGCAAATTGACTGTGGAAGAGCTGACCGGCGGTAACTTC
ACCATTACCAACGGCGGCGTATTCGGTTCACTGATGTCTACCCCGATCATCAACCCACCGCAGAGCGCCATCCTGGGCAT
GCACGCCATTAAAGATCGCCCAATGGCGGTCAAAGGCCAGGTTGTGATCCTGCCGATGATGTATCTGGCACTGTCTTATG
ACCATCGCCTGATCGACGGTAAAGAATCCGTGGGTTACCTGGTGACGGTTAAAGAGATGCTGGAAGATCCGGCTCGTCTG
CTGCTGGACGTATAA

Upstream 100 bases:

>100_bases
CTGCCTCTCCGGCAGTGGGTTATATGTCCGTACACCAGAAGCAGCAACAGGCTCTGGTTAATGACGCGCTGAATATTGTT
AAAGATTAAGGGAAAGCTAA

Downstream 100 bases:

>100_bases
CCCTGATGGGCGCGGTACGCTGCGCCCACACTCTGTGCTATGTGGCCGACTAAAGTCATGCGGTTTTCCGCCAAAATGGC
TCGGCTAAAACCTTCAGAAC

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSSVDILVPDLPESVADATVATWHKKPGDSVQRDEVLVEIETDKVVLEVPASEAGILDAIVEEEGATVLSRQILGRIRPG
DSSGKPTEEKSQAKEATPAQRATASLEEESNDALSPAIRRLIAEHDLDAAAIKGSGVGGRITREDVEAHLANGKKADKPA
AVEAAPQPALSGRSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEINMQPIMDMRKQYGEAFEKRHGVRLGFMSFYIKA
VVEALKRFPEVNASIDGTDVVYHNYFDISIAVSTPRGLVTPVLRDVDSMSMADIEKKIKELAVKGRDGKLTVEELTGGNF
TITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVKGQVVILPMMYLALSYDHRLIDGKESVGYLVTVKEMLEDPARL
LLDV

Sequences:

>Translated_404_residues
MSSVDILVPDLPESVADATVATWHKKPGDSVQRDEVLVEIETDKVVLEVPASEAGILDAIVEEEGATVLSRQILGRIRPG
DSSGKPTEEKSQAKEATPAQRATASLEEESNDALSPAIRRLIAEHDLDAAAIKGSGVGGRITREDVEAHLANGKKADKPA
AVEAAPQPALSGRSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEINMQPIMDMRKQYGEAFEKRHGVRLGFMSFYIKA
VVEALKRFPEVNASIDGTDVVYHNYFDISIAVSTPRGLVTPVLRDVDSMSMADIEKKIKELAVKGRDGKLTVEELTGGNF
TITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVKGQVVILPMMYLALSYDHRLIDGKESVGYLVTVKEMLEDPARL
LLDV
>Mature_403_residues
SSVDILVPDLPESVADATVATWHKKPGDSVQRDEVLVEIETDKVVLEVPASEAGILDAIVEEEGATVLSRQILGRIRPGD
SSGKPTEEKSQAKEATPAQRATASLEEESNDALSPAIRRLIAEHDLDAAAIKGSGVGGRITREDVEAHLANGKKADKPAA
VEAAPQPALSGRSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEINMQPIMDMRKQYGEAFEKRHGVRLGFMSFYIKAV
VEALKRFPEVNASIDGTDVVYHNYFDISIAVSTPRGLVTPVLRDVDSMSMADIEKKIKELAVKGRDGKLTVEELTGGNFT
ITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVKGQVVILPMMYLALSYDHRLIDGKESVGYLVTVKEMLEDPARLL
LDV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=234, Percent_Identity=59.4017094017094, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI31711992, Length=432, Percent_Identity=29.3981481481481, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI203098753, Length=453, Percent_Identity=28.476821192053, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI203098816, Length=453, Percent_Identity=28.476821192053, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI110671329, Length=434, Percent_Identity=27.1889400921659, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=33.9285714285714, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI1786946, Length=405, Percent_Identity=82.7160493827161, Blast_Score=682, Evalue=0.0,
Organism=Escherichia coli, GI1786305, Length=404, Percent_Identity=30.9405940594059, Blast_Score=161, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI25146366, Length=410, Percent_Identity=42.6829268292683, Blast_Score=315, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI17560088, Length=437, Percent_Identity=31.5789473684211, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=420, Percent_Identity=26.1904761904762, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=315, Percent_Identity=29.2063492063492, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320352, Length=411, Percent_Identity=42.5790754257908, Blast_Score=325, Evalue=7e-90,
Organism=Saccharomyces cerevisiae, GI6324258, Length=454, Percent_Identity=25.9911894273128, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24645909, Length=229, Percent_Identity=59.825327510917, Blast_Score=291, Evalue=5e-79,
Organism=Drosophila melanogaster, GI18859875, Length=432, Percent_Identity=29.1666666666667, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43946; Mature: 43815

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVDILVPDLPESVADATVATWHKKPGDSVQRDEVLVEIETDKVVLEVPASEAGILDAI
CCCCCEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEEEEECCCCCCHHHHHH
VEEEGATVLSRQILGRIRPGDSSGKPTEEKSQAKEATPAQRATASLEEESNDALSPAIRR
HHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHH
LIAEHDLDAAAIKGSGVGGRITREDVEAHLANGKKADKPAAVEAAPQPALSGRSEKRVPM
HHHHCCCCHHEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
TRLRKRVAERLLEAKNSTAMLTTFNEINMQPIMDMRKQYGEAFEKRHGVRLGFMSFYIKA
HHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHH
VVEALKRFPEVNASIDGTDVVYHNYFDISIAVSTPRGLVTPVLRDVDSMSMADIEKKIKE
HHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEEECCCHHHHHHHHHHHCHHHHHHHHHHHH
LAVKGRDGKLTVEELTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVKGQ
HHHCCCCCCEEEEEECCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEECCC
VVILPMMYLALSYDHRLIDGKESVGYLVTVKEMLEDPARLLLDV
CHHHHHHHHHHHCCCCEECCCCCCCCEEEHHHHHCCHHHHHCCC
>Mature Secondary Structure 
SSVDILVPDLPESVADATVATWHKKPGDSVQRDEVLVEIETDKVVLEVPASEAGILDAI
CCCCEECCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCEEEEECCCCCCHHHHHH
VEEEGATVLSRQILGRIRPGDSSGKPTEEKSQAKEATPAQRATASLEEESNDALSPAIRR
HHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHH
LIAEHDLDAAAIKGSGVGGRITREDVEAHLANGKKADKPAAVEAAPQPALSGRSEKRVPM
HHHHCCCCHHEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
TRLRKRVAERLLEAKNSTAMLTTFNEINMQPIMDMRKQYGEAFEKRHGVRLGFMSFYIKA
HHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHH
VVEALKRFPEVNASIDGTDVVYHNYFDISIAVSTPRGLVTPVLRDVDSMSMADIEKKIKE
HHHHHHHCCCCCCCCCCCEEEEEEEEEEEEEEECCCHHHHHHHHHHHCHHHHHHHHHHHH
LAVKGRDGKLTVEELTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVKGQ
HHHCCCCCCEEEEEECCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEECCC
VVILPMMYLALSYDHRLIDGKESVGYLVTVKEMLEDPARLLLDV
CHHHHHHHHHHHCCCCEECCCCCCCCEEEHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]