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The map label for this gene is nei [H]

Identifier: 157369504

GI number: 157369504

Start: 1386032

End: 1386823

Strand: Direct

Name: nei [H]

Synonym: Spro_1261

Alternate gene names: 157369504

Gene position: 1386032-1386823 (Clockwise)

Preceding gene: 157369503

Following gene: 157369506

Centisome position: 25.44

GC content: 59.34

Gene sequence:

>792_bases
ATGCCGGAAGGACCGGAAATTCGCCGGGCGGCGGATGCACTGGCGGCGGCGGTGATCGACCAGCCGCTGACCGAAGTCGG
TTTTGCCTTTCCCCAGCTTAAACATTACCGCGACCGGTTAATCGGCGAGCGTATTATCGCTATTGAACCACGGGGTAAGG
CACTGCTGACCCATTTTTCCAATGGTCTGACGCTATATAGCCATAACCAGTTGTATGGCGTGTGGAAAGTGGTTAAAGCA
GGGGAGACGCCGGAAACCAAACGGGATCTGCGCGTGCGGCTGGAAACGGCGGATCGGGCGATTTTGCTGTACAGCGCCTC
GGAGATTACCGTCGGGCCGCGCGAGGAAATTGAGCAGCATCCGTTCTTGCAACGTATCGGCCCCGACGTACTGGATATGA
CGTTAACGGTGGCCGCGGTGGAGCAACGGCTGCTGTCACCGGCATTTCGCCGCAGGCAACTGGGCGGCATGTTGCTCGAT
CAGGCGTTTCTCGCCGGGTTGGGCAACTACCTGCGGGCCGAGATCCTCTGGCAGGCAGAGCTGGCCCCCCAGCATAAGCC
ACAGGATCTGGCGCCGGAAACCTTGCAGCGTTTGGCGGAGGCGTTGCTGGCGGTACCGCGCTTGTCTTATCAGACGCGTG
GGCAGGTGGATGAGAACCGCCATCATGGCGCGTTGTTCAGCTTTAAGGTGTTCCACCGCAGTGGTGAACCTTGCGAGCGT
TGTGGTGCGATGATCGAACGCACGACGCTGTCTTCAAGGCCCTTTTATTGGTGCCCAGGTTGCCAGAAATAG

Upstream 100 bases:

>100_bases
AGGTTGAACACGACCTGAAAGTAGTCGGTGGCGCGACGCATTAATCGCAGTTATTCCCCGGTGCGGCCTGCTGCACCGGG
GTCCATTCAGGAGTTTTCAT

Downstream 100 bases:

>100_bases
GTTATTCAATGTTTCCCCTCACCCCGACCCTCTCCCCAGGGAGAGGGCAAAAAAAAACGCCGCCCATCGGGGCGGCGTCT
TGCTGCAATACGTAGGGTAA

Product: endonuclease VIII

Products: NA

Alternate protein names: DNA glycosylase/AP lyase Nei; DNA-(apurinic or apyrimidinic site) lyase Nei; Endonuclease VIII [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MPEGPEIRRAADALAAAVIDQPLTEVGFAFPQLKHYRDRLIGERIIAIEPRGKALLTHFSNGLTLYSHNQLYGVWKVVKA
GETPETKRDLRVRLETADRAILLYSASEITVGPREEIEQHPFLQRIGPDVLDMTLTVAAVEQRLLSPAFRRRQLGGMLLD
QAFLAGLGNYLRAEILWQAELAPQHKPQDLAPETLQRLAEALLAVPRLSYQTRGQVDENRHHGALFSFKVFHRSGEPCER
CGAMIERTTLSSRPFYWCPGCQK

Sequences:

>Translated_263_residues
MPEGPEIRRAADALAAAVIDQPLTEVGFAFPQLKHYRDRLIGERIIAIEPRGKALLTHFSNGLTLYSHNQLYGVWKVVKA
GETPETKRDLRVRLETADRAILLYSASEITVGPREEIEQHPFLQRIGPDVLDMTLTVAAVEQRLLSPAFRRRQLGGMLLD
QAFLAGLGNYLRAEILWQAELAPQHKPQDLAPETLQRLAEALLAVPRLSYQTRGQVDENRHHGALFSFKVFHRSGEPCER
CGAMIERTTLSSRPFYWCPGCQK
>Mature_262_residues
PEGPEIRRAADALAAAVIDQPLTEVGFAFPQLKHYRDRLIGERIIAIEPRGKALLTHFSNGLTLYSHNQLYGVWKVVKAG
ETPETKRDLRVRLETADRAILLYSASEITVGPREEIEQHPFLQRIGPDVLDMTLTVAAVEQRLLSPAFRRRQLGGMLLDQ
AFLAGLGNYLRAEILWQAELAPQHKPQDLAPETLQRLAEALLAVPRLSYQTRGQVDENRHHGALFSFKVFHRSGEPCERC
GAMIERTTLSSRPFYWCPGCQK

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothy

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger [H]

Homologues:

Organism=Escherichia coli, GI1786932, Length=262, Percent_Identity=69.8473282442748, Blast_Score=386, Evalue=1e-109,
Organism=Escherichia coli, GI1790066, Length=281, Percent_Identity=25.9786476868327, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR012319
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663 [H]

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 29673; Mature: 29541

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS01242 ZF_FPG_1 ; PS51066 ZF_FPG_2 ; PS51068 FPG_CAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEGPEIRRAADALAAAVIDQPLTEVGFAFPQLKHYRDRLIGERIIAIEPRGKALLTHFS
CCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEHHHC
NGLTLYSHNQLYGVWKVVKAGETPETKRDLRVRLETADRAILLYSASEITVGPREEIEQH
CCEEEEECCCHHHHHHHHHCCCCCCCCHHHEEEEECCCCEEEEEECCCCCCCCHHHHHHC
PFLQRIGPDVLDMTLTVAAVEQRLLSPAFRRRQLGGMLLDQAFLAGLGNYLRAEILWQAE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHH
LAPQHKPQDLAPETLQRLAEALLAVPRLSYQTRGQVDENRHHGALFSFKVFHRSGEPCER
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHH
CGAMIERTTLSSRPFYWCPGCQK
HHHHHHHHHCCCCCEEECCCCCC
>Mature Secondary Structure 
PEGPEIRRAADALAAAVIDQPLTEVGFAFPQLKHYRDRLIGERIIAIEPRGKALLTHFS
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEEHHHC
NGLTLYSHNQLYGVWKVVKAGETPETKRDLRVRLETADRAILLYSASEITVGPREEIEQH
CCEEEEECCCHHHHHHHHHCCCCCCCCHHHEEEEECCCCEEEEEECCCCCCCCHHHHHHC
PFLQRIGPDVLDMTLTVAAVEQRLLSPAFRRRQLGGMLLDQAFLAGLGNYLRAEILWQAE
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHH
LAPQHKPQDLAPETLQRLAEALLAVPRLSYQTRGQVDENRHHGALFSFKVFHRSGEPCER
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHH
CGAMIERTTLSSRPFYWCPGCQK
HHHHHHHHHCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA