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The map label for this gene is acrA [H]

Identifier: 157369370

GI number: 157369370

Start: 1240865

End: 1242052

Strand: Reverse

Name: acrA [H]

Synonym: Spro_1127

Alternate gene names: 157369370

Gene position: 1242052-1240865 (Counterclockwise)

Preceding gene: 157369374

Following gene: 157369369

Centisome position: 22.79

GC content: 56.06

Gene sequence:

>1188_bases
ATGAACAAAAACAGAGGGTTAACGCCTCTGGCGGCAGTTCTGATGCTTTCAGGCAGCTTAATGCTTACAGGATGTAACGA
TAAAGAAACCCAACAGCAAGGCGCCCAACCACCGGCTCCCGAAGTGGGTGTAGTGACTTTGAAGGCCGAGCCTCTCAATA
TGACTACCGATCTTCCTGGCCGCACCGCTGCGTATCGTATCGCCGAAGTTCGCCCTCAGGTCAGCGGTATTATCCTGAAA
CGCAACTTTGTTGAAGGCAGTGATATCAAGGCAGGCACTTCCCTGTATCAGATTGATCCTGCTACCTATCAGGCAAGCTA
TGACAGCGCCAAAGGCGATCTGGCCAAAGCACAGGCCAGCGCTTCTATCGCGCGCGTGACGGTCAACCGCTACAAGCCCT
TGCTGGGCACCAGCTACATCAGCAAGCAGGATTACGATACAGCCGTTTCCAACCTGCAGCAGGCAGATGCCGCTGTGATA
GCCGCAAAAGCGGCGGTTGAAACTGCGCGTATCAACCTGGCTTACACTAAAGTGACGTCCCCAATCTCCGGGCGTATCGG
CAAATCTTCCGTAACCGAAGGCGCGCTGGTCAGCAACGGTCAAACCACGGCGCTGTCTACCGTGCAACAGTTGGATCCGA
TTTACGTCGACGTCACTCAGTCGAGCAACGACTTTCTGCGCCTGAAGCAAGAATTGGCCAGCGGTGCGCTGAAACAGGAA
AACGGCAAGGCCAAGGTGAAACTGCTGCTGGAGAACGGCGCGCAGTACGCTCAGGAAGGCACGCTGGAATTCTCTGACGT
CACCGTGGATGAAACCACCGGTTCCATCACCATTCGCGCCCTGTTCCCTAACCCGAACGATACGCTGCTGCCAGGTATGT
TCGTTCGCGCCCGTCTGGACGAAGGCGTGCGCAGCGATGCATTGCTGGTGCCACAGCAGGGTGTAACGCGTAACCCGCGT
GGCGAAGCTACCGCGATGGTAGTGGGCGCTGACGATAAAGTCGAAATGCGCACGCTGAAAGCGGACCAGGCGATTGGTGA
CAAATGGTTGGTTACCGACGGCTTGAAAGCCGGCGACCGCGTAATTGTCACTGGCCTGATGAAAGTCCGCCCAGGCGCTC
AGGTAAAAGTGCAGGAAGTTGACACTCAGGCGCAAAAGCAGCCGCAGTCTGAAGCGCAGAAGTCATAA

Upstream 100 bases:

>100_bases
TATGTATGTACCATAGCACGCACATATAATTAACGCAGCAATGGGTTTTAAAGCTTGTGATCCATTGATCATTTTGAAAT
CGGACACTTGAGGTTTTTCT

Downstream 100 bases:

>100_bases
AAAGGAGCCGGTAAGACATGGCTAAGTTCTTTATAGATCGCCCTATTTTCGCCTGGGTAATCGCCATCATCGTCATGTTG
GCGGGTGTGCTGTCAATAAT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MNKNRGLTPLAAVLMLSGSLMLTGCNDKETQQQGAQPPAPEVGVVTLKAEPLNMTTDLPGRTAAYRIAEVRPQVSGIILK
RNFVEGSDIKAGTSLYQIDPATYQASYDSAKGDLAKAQASASIARVTVNRYKPLLGTSYISKQDYDTAVSNLQQADAAVI
AAKAAVETARINLAYTKVTSPISGRIGKSSVTEGALVSNGQTTALSTVQQLDPIYVDVTQSSNDFLRLKQELASGALKQE
NGKAKVKLLLENGAQYAQEGTLEFSDVTVDETTGSITIRALFPNPNDTLLPGMFVRARLDEGVRSDALLVPQQGVTRNPR
GEATAMVVGADDKVEMRTLKADQAIGDKWLVTDGLKAGDRVIVTGLMKVRPGAQVKVQEVDTQAQKQPQSEAQKS

Sequences:

>Translated_395_residues
MNKNRGLTPLAAVLMLSGSLMLTGCNDKETQQQGAQPPAPEVGVVTLKAEPLNMTTDLPGRTAAYRIAEVRPQVSGIILK
RNFVEGSDIKAGTSLYQIDPATYQASYDSAKGDLAKAQASASIARVTVNRYKPLLGTSYISKQDYDTAVSNLQQADAAVI
AAKAAVETARINLAYTKVTSPISGRIGKSSVTEGALVSNGQTTALSTVQQLDPIYVDVTQSSNDFLRLKQELASGALKQE
NGKAKVKLLLENGAQYAQEGTLEFSDVTVDETTGSITIRALFPNPNDTLLPGMFVRARLDEGVRSDALLVPQQGVTRNPR
GEATAMVVGADDKVEMRTLKADQAIGDKWLVTDGLKAGDRVIVTGLMKVRPGAQVKVQEVDTQAQKQPQSEAQKS
>Mature_395_residues
MNKNRGLTPLAAVLMLSGSLMLTGCNDKETQQQGAQPPAPEVGVVTLKAEPLNMTTDLPGRTAAYRIAEVRPQVSGIILK
RNFVEGSDIKAGTSLYQIDPATYQASYDSAKGDLAKAQASASIARVTVNRYKPLLGTSYISKQDYDTAVSNLQQADAAVI
AAKAAVETARINLAYTKVTSPISGRIGKSSVTEGALVSNGQTTALSTVQQLDPIYVDVTQSSNDFLRLKQELASGALKQE
NGKAKVKLLLENGAQYAQEGTLEFSDVTVDETTGSITIRALFPNPNDTLLPGMFVRARLDEGVRSDALLVPQQGVTRNPR
GEATAMVVGADDKVEMRTLKADQAIGDKWLVTDGLKAGDRVIVTGLMKVRPGAQVKVQEVDTQAQKQPQSEAQKS

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=393, Percent_Identity=77.6081424936387, Blast_Score=583, Evalue=1e-168,
Organism=Escherichia coli, GI1789665, Length=376, Percent_Identity=65.6914893617021, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1789929, Length=376, Percent_Identity=57.7127659574468, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI87082036, Length=334, Percent_Identity=29.6407185628743, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI87081796, Length=366, Percent_Identity=23.4972677595628, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081951, Length=117, Percent_Identity=35.042735042735, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42106; Mature: 42106

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNRGLTPLAAVLMLSGSLMLTGCNDKETQQQGAQPPAPEVGVVTLKAEPLNMTTDLPG
CCCCCCCCHHHHHHHHCCCEEEECCCCHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCC
RTAAYRIAEVRPQVSGIILKRNFVEGSDIKAGTSLYQIDPATYQASYDSAKGDLAKAQAS
CHHHEEHHHCCCCHHEEEEEECCCCCCCCCCCCEEEEECCCCEECCCCCCCCHHHHHHHH
ASIARVTVNRYKPLLGTSYISKQDYDTAVSNLQQADAAVIAAKAAVETARINLAYTKVTS
HHEEEEEHHHCCCHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECC
PISGRIGKSSVTEGALVSNGQTTALSTVQQLDPIYVDVTQSSNDFLRLKQELASGALKQE
CCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCC
NGKAKVKLLLENGAQYAQEGTLEFSDVTVDETTGSITIRALFPNPNDTLLPGMFVRARLD
CCCEEEEEEEECCCHHHHCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHC
EGVRSDALLVPQQGVTRNPRGEATAMVVGADDKVEMRTLKADQAIGDKWLVTDGLKAGDR
CCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCEEEEEEHHHHHCCCEEEEECCCCCCCE
VIVTGLMKVRPGAQVKVQEVDTQAQKQPQSEAQKS
EEEEEEEEECCCCCEEEEECCCHHHCCCHHHHCCC
>Mature Secondary Structure
MNKNRGLTPLAAVLMLSGSLMLTGCNDKETQQQGAQPPAPEVGVVTLKAEPLNMTTDLPG
CCCCCCCCHHHHHHHHCCCEEEECCCCHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCC
RTAAYRIAEVRPQVSGIILKRNFVEGSDIKAGTSLYQIDPATYQASYDSAKGDLAKAQAS
CHHHEEHHHCCCCHHEEEEEECCCCCCCCCCCCEEEEECCCCEECCCCCCCCHHHHHHHH
ASIARVTVNRYKPLLGTSYISKQDYDTAVSNLQQADAAVIAAKAAVETARINLAYTKVTS
HHEEEEEHHHCCCHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEECC
PISGRIGKSSVTEGALVSNGQTTALSTVQQLDPIYVDVTQSSNDFLRLKQELASGALKQE
CCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCCCC
NGKAKVKLLLENGAQYAQEGTLEFSDVTVDETTGSITIRALFPNPNDTLLPGMFVRARLD
CCCEEEEEEEECCCHHHHCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCCHHHHHHHC
EGVRSDALLVPQQGVTRNPRGEATAMVVGADDKVEMRTLKADQAIGDKWLVTDGLKAGDR
CCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCEEEEEEHHHHHCCCEEEEECCCCCCCE
VIVTGLMKVRPGAQVKVQEVDTQAQKQPQSEAQKS
EEEEEEEEECCCCCEEEEECCCHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]