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The map label for this gene is sbcD [H]

Identifier: 157369283

GI number: 157369283

Start: 1143222

End: 1144454

Strand: Reverse

Name: sbcD [H]

Synonym: Spro_1040

Alternate gene names: 157369283

Gene position: 1144454-1143222 (Counterclockwise)

Preceding gene: 157369290

Following gene: 157369282

Centisome position: 21.0

GC content: 58.8

Gene sequence:

>1233_bases
ATGCGCCTGATCCACACCTCTGACTGGCATCTTGGCCAGTATTTTTTCACCAAGAGCCGCGCCGCCGAACATCAGGCTTT
TCTGCGCTGGCTGATCGAACAGATCGAACAACAGCAGGTGGATGCACTGATCGTCGCCGGGGACCTGTTCGATACCGGCT
CGCCGCCAAGTTATGCCCGTGAGCTGTATAACCGATTCGTGGTGGATCTGCAGCGCACCGGCTGCCAGTTGGTGGTGCTG
GGCGGTAACCACGACTCCGTGGCAACCTTGAATGAGTCGCGTGAATTGCTTTCCTGCCTGAATACCACCGTGATTGCCAA
CGCACAGAGCGAAACCGAACAGCAAATTCTGGTGCTGAATCAGCGCGATGGTCAGCCGGGTGCGGTACTTTGTGCCATCC
CTTTCCTGCGCCCACGCGATCTGCTCACCAGCCGCGCCGGCGACTCCGGGGTGCAAAAACAGCAGGCGCTGCAAGAGGCT
ATCGCCGACCATTATCAAAGGCTGTACCAAGCGGCCTGCGCGCGTCGCGATGCGCTGGGCTTGCCACTGCCGATTGTTGC
CACCGGCCACCTGACCACCGTGGGCGTCACCACCTCCGATTCGGTGCGCGATATCTACATTGGCACGCTGGACGCCTTCC
CGGCCCAGGCCTTCCCACCGGCCGACTACATCGCGCTGGGGCATATACACCGCGCGCAGAACGTCGCTAAATCCGAACAT
ATTCGCTACAGCGGCTCGCCGATCCCACTCAGTTTTGACGAGTTGGGCAAAGCCAAAAGCGTGTTTATGGTGGATTTCAG
CGCGGGCGCTCTGCAACAGGTCACTGCGCTGGAGATCCCCAGCTTCCAACCGATGCAGCTTATCAAGGGTGATTTGGCGC
AGATCGAACAACAATTGCAGCAGTTCGCCGATTATCAGGGTGAACTACCGGTGTGGCTGGACATTGAAGTGGCAACACAG
GACTACCTTAGCGACATTCAGCGCCGCATCCAGTTACTGGCCGATCAACTGCCGGTAGAAGTGGTGCTGTTGCGCCGCAG
CAAAGAACAGCGGCGCCAGGCCATTGAACAGCAGGACAAGGAAACGCTCAATGAACTGAGCGTCAACGAGGTGTTCGAAC
GGCGGCTGGCGCAGGAGCCTGAAATGGCCGAAGAACGTCGGCAACGAATGAACCAAATGTTCCAGCAGGTGGTCGAGTCC
GTGCGGCACAACGACCAGGAGCCGAGCCAATGA

Upstream 100 bases:

>100_bases
GGGTGTCATAGGCAGTAAGCATAGCGATTATTTCTAATAATACAGAAACATCATATTCCCTTTATAATCCCTGCCATTAC
TCTATCGCCCCGGGAGTGTC

Downstream 100 bases:

>100_bases
AAATCCTCAGCCTGAGATTAAAAAACCTTAACTCCCTGCAGGGTGAATGGAAGATCGATTTTACCGCCGAGCCCTTTGCC
AGTAATGGCCTATTCGCCAT

Product: exonuclease subunit SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MRLIHTSDWHLGQYFFTKSRAAEHQAFLRWLIEQIEQQQVDALIVAGDLFDTGSPPSYARELYNRFVVDLQRTGCQLVVL
GGNHDSVATLNESRELLSCLNTTVIANAQSETEQQILVLNQRDGQPGAVLCAIPFLRPRDLLTSRAGDSGVQKQQALQEA
IADHYQRLYQAACARRDALGLPLPIVATGHLTTVGVTTSDSVRDIYIGTLDAFPAQAFPPADYIALGHIHRAQNVAKSEH
IRYSGSPIPLSFDELGKAKSVFMVDFSAGALQQVTALEIPSFQPMQLIKGDLAQIEQQLQQFADYQGELPVWLDIEVATQ
DYLSDIQRRIQLLADQLPVEVVLLRRSKEQRRQAIEQQDKETLNELSVNEVFERRLAQEPEMAEERRQRMNQMFQQVVES
VRHNDQEPSQ

Sequences:

>Translated_410_residues
MRLIHTSDWHLGQYFFTKSRAAEHQAFLRWLIEQIEQQQVDALIVAGDLFDTGSPPSYARELYNRFVVDLQRTGCQLVVL
GGNHDSVATLNESRELLSCLNTTVIANAQSETEQQILVLNQRDGQPGAVLCAIPFLRPRDLLTSRAGDSGVQKQQALQEA
IADHYQRLYQAACARRDALGLPLPIVATGHLTTVGVTTSDSVRDIYIGTLDAFPAQAFPPADYIALGHIHRAQNVAKSEH
IRYSGSPIPLSFDELGKAKSVFMVDFSAGALQQVTALEIPSFQPMQLIKGDLAQIEQQLQQFADYQGELPVWLDIEVATQ
DYLSDIQRRIQLLADQLPVEVVLLRRSKEQRRQAIEQQDKETLNELSVNEVFERRLAQEPEMAEERRQRMNQMFQQVVES
VRHNDQEPSQ
>Mature_410_residues
MRLIHTSDWHLGQYFFTKSRAAEHQAFLRWLIEQIEQQQVDALIVAGDLFDTGSPPSYARELYNRFVVDLQRTGCQLVVL
GGNHDSVATLNESRELLSCLNTTVIANAQSETEQQILVLNQRDGQPGAVLCAIPFLRPRDLLTSRAGDSGVQKQQALQEA
IADHYQRLYQAACARRDALGLPLPIVATGHLTTVGVTTSDSVRDIYIGTLDAFPAQAFPPADYIALGHIHRAQNVAKSEH
IRYSGSPIPLSFDELGKAKSVFMVDFSAGALQQVTALEIPSFQPMQLIKGDLAQIEQQLQQFADYQGELPVWLDIEVATQ
DYLSDIQRRIQLLADQLPVEVVLLRRSKEQRRQAIEQQDKETLNELSVNEVFERRLAQEPEMAEERRQRMNQMFQQVVES
VRHNDQEPSQ

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=401, Percent_Identity=63.0922693266833, Blast_Score=524, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: 3.1.-.-

Molecular weight: Translated: 46254; Mature: 46254

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLIHTSDWHLGQYFFTKSRAAEHQAFLRWLIEQIEQQQVDALIVAGDLFDTGSPPSYAR
CCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHH
ELYNRFVVDLQRTGCQLVVLGGNHDSVATLNESRELLSCLNTTVIANAQSETEQQILVLN
HHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEEE
QRDGQPGAVLCAIPFLRPRDLLTSRAGDSGVQKQQALQEAIADHYQRLYQAACARRDALG
CCCCCCCEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LPLPIVATGHLTTVGVTTSDSVRDIYIGTLDAFPAQAFPPADYIALGHIHRAQNVAKSEH
CCCCEEECCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
IRYSGSPIPLSFDELGKAKSVFMVDFSAGALQQVTALEIPSFQPMQLIKGDLAQIEQQLQ
EEECCCCCCCCHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
QFADYQGELPVWLDIEVATQDYLSDIQRRIQLLADQLPVEVVLLRRSKEQRRQAIEQQDK
HHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHH
ETLNELSVNEVFERRLAQEPEMAEERRQRMNQMFQQVVESVRHNDQEPSQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRLIHTSDWHLGQYFFTKSRAAEHQAFLRWLIEQIEQQQVDALIVAGDLFDTGSPPSYAR
CCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCCHHHHH
ELYNRFVVDLQRTGCQLVVLGGNHDSVATLNESRELLSCLNTTVIANAQSETEQQILVLN
HHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEEE
QRDGQPGAVLCAIPFLRPRDLLTSRAGDSGVQKQQALQEAIADHYQRLYQAACARRDALG
CCCCCCCEEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LPLPIVATGHLTTVGVTTSDSVRDIYIGTLDAFPAQAFPPADYIALGHIHRAQNVAKSEH
CCCCEEECCCEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
IRYSGSPIPLSFDELGKAKSVFMVDFSAGALQQVTALEIPSFQPMQLIKGDLAQIEQQLQ
EEECCCCCCCCHHHHCCCCEEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
QFADYQGELPVWLDIEVATQDYLSDIQRRIQLLADQLPVEVVLLRRSKEQRRQAIEQQDK
HHHCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHH
ETLNELSVNEVFERRLAQEPEMAEERRQRMNQMFQQVVESVRHNDQEPSQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]