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The map label for this gene is iap [H]

Identifier: 157369060

GI number: 157369060

Start: 910418

End: 911491

Strand: Reverse

Name: iap [H]

Synonym: Spro_0815

Alternate gene names: 157369060

Gene position: 911491-910418 (Counterclockwise)

Preceding gene: 157369067

Following gene: 157369059

Centisome position: 16.73

GC content: 56.33

Gene sequence:

>1074_bases
ATGTTTTCCCGCTTTTATCTAAAGGCCGGCCTGCTGGTGGCAGTGCTCGGCGGTGTTTTCAGCGTTTCTGCCGCCACACA
GCCCGCGAAAGATCTCCCGATGGGTAAATTCGCCGCTGACCAAATCCGCCATATCGCTACCTATTTCCCCGGCCGTATGG
CAGGCAGCCCGGCCGAACTGCTCACCGCAGACTATCTAAACCAGCAGTTCAGCAAGATGGGTTATCAGAGCGATATCCGC
AGCTTCAATACCCGTTATCTCTACACCAGTAAAGACGGTAAAAAGAACTGGAATAATGTTACCGCCAGCTCGGTGATCGC
CGCTAAAAATGGCAGCAATCCGCAGCAAATTCTGATCGTGGCGCATTTTGATACCTATACGCCACAAAGCGACGCCGACC
TGGATAACAACCTCGGCGGCCTGACGCTACAGGGCGTCGATGACAATGCCTCCGGTATCGGTGTAATGCTGGAGTTGGCA
GAGCGCCTGAAGAACATCCCCACGGCTTATGGCCTGCGCTTTGTTGCCACCAGCGCCGAAGAAATCGGCTCATTGGGGGC
GCAGAACTATCTGCAACGTATGAGCCCGGAAGAAAAACGCAATACTGTGCTGGTGATTAACCTCGACAGCCTGATCACCG
GCGATCGCCTGTATTTCAACGCTGGCCGCAACACGCCACCCAAGTTGGCGAAACAGAGCCGCGATCGCGCACTGGACATC
GCCCACCGCTATGGCATTACCGCCGCCACTAACCCAGGCAGCCAGGCGCACCCGAAGGGCACCGGCTGCTGCTCCGATCA
GGAGGTGTTTGATAAAGCCGGTATTCCGGTGCTGTCCGTCGAAGCCACCAACTGGTCGTTGGGCAAGAAAGACGGCTATC
AGCAGCGTGCCGTCAGCCCGCACTTCCCACAGGGCATAAGTTGGCACCGCCCGCAGTACGATAATCTGCAATACCTGGAG
CGTAATTTGCCAGGACGGATCGATAAACGTAGCCGCGAGAGCGTGCAGATCCTGTTGCCGTTGATCAAAGAGCTGGCGCA
GGCGCATCCACCTAAAGCGCCAAAGAAAAAGTAG

Upstream 100 bases:

>100_bases
TTTCAGAGCGGTGGAAATTGTTTAATCTGCCGCCGAGTCTCATACTGAGGGCCGCAAAAAGAATCGGTATTACCGACATT
AAAAAGAAGGATTTTTCGGT

Downstream 100 bases:

>100_bases
TCTTATCGGGGGCAAAATTCAGTACAAATTTTGTACCCCAATGCTCGACTGAGTATACTGTTGCCAGGATTTCGCAGCAG
GAGCCCGACTGATGAGCACA

Product: alkaline phosphatase isozyme conversion aminopeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MFSRFYLKAGLLVAVLGGVFSVSAATQPAKDLPMGKFAADQIRHIATYFPGRMAGSPAELLTADYLNQQFSKMGYQSDIR
SFNTRYLYTSKDGKKNWNNVTASSVIAAKNGSNPQQILIVAHFDTYTPQSDADLDNNLGGLTLQGVDDNASGIGVMLELA
ERLKNIPTAYGLRFVATSAEEIGSLGAQNYLQRMSPEEKRNTVLVINLDSLITGDRLYFNAGRNTPPKLAKQSRDRALDI
AHRYGITAATNPGSQAHPKGTGCCSDQEVFDKAGIPVLSVEATNWSLGKKDGYQQRAVSPHFPQGISWHRPQYDNLQYLE
RNLPGRIDKRSRESVQILLPLIKELAQAHPPKAPKKK

Sequences:

>Translated_357_residues
MFSRFYLKAGLLVAVLGGVFSVSAATQPAKDLPMGKFAADQIRHIATYFPGRMAGSPAELLTADYLNQQFSKMGYQSDIR
SFNTRYLYTSKDGKKNWNNVTASSVIAAKNGSNPQQILIVAHFDTYTPQSDADLDNNLGGLTLQGVDDNASGIGVMLELA
ERLKNIPTAYGLRFVATSAEEIGSLGAQNYLQRMSPEEKRNTVLVINLDSLITGDRLYFNAGRNTPPKLAKQSRDRALDI
AHRYGITAATNPGSQAHPKGTGCCSDQEVFDKAGIPVLSVEATNWSLGKKDGYQQRAVSPHFPQGISWHRPQYDNLQYLE
RNLPGRIDKRSRESVQILLPLIKELAQAHPPKAPKKK
>Mature_357_residues
MFSRFYLKAGLLVAVLGGVFSVSAATQPAKDLPMGKFAADQIRHIATYFPGRMAGSPAELLTADYLNQQFSKMGYQSDIR
SFNTRYLYTSKDGKKNWNNVTASSVIAAKNGSNPQQILIVAHFDTYTPQSDADLDNNLGGLTLQGVDDNASGIGVMLELA
ERLKNIPTAYGLRFVATSAEEIGSLGAQNYLQRMSPEEKRNTVLVINLDSLITGDRLYFNAGRNTPPKLAKQSRDRALDI
AHRYGITAATNPGSQAHPKGTGCCSDQEVFDKAGIPVLSVEATNWSLGKKDGYQQRAVSPHFPQGISWHRPQYDNLQYLE
RNLPGRIDKRSRESVQILLPLIKELAQAHPPKAPKKK

Specific function: This protein, presumably an aminopeptidase, mediates the conversion of E.coli alkaline phosphatase isozyme 1, to isozymes 2 and 3 by removing, one by one, the two amino-terminal arginine residues [H]

COG id: COG2234

COG function: function code R; Predicted aminopeptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M28 family. M28C subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789111, Length=315, Percent_Identity=66.3492063492063, Blast_Score=454, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007484 [H]

Pfam domain/function: PF04389 Peptidase_M28 [H]

EC number: 3.4.11.- [C]

Molecular weight: Translated: 39240; Mature: 39240

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSRFYLKAGLLVAVLGGVFSVSAATQPAKDLPMGKFAADQIRHIATYFPGRMAGSPAEL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
LTADYLNQQFSKMGYQSDIRSFNTRYLYTSKDGKKNWNNVTASSVIAAKNGSNPQQILIV
HHHHHHHHHHHHCCHHHHHHHCCCEEEEECCCCCCCCCCCCHHHEEEECCCCCCEEEEEE
AHFDTYTPQSDADLDNNLGGLTLQGVDDNASGIGVMLELAERLKNIPTAYGLRFVATSAE
EEECCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHEEEECCHH
EIGSLGAQNYLQRMSPEEKRNTVLVINLDSLITGDRLYFNAGRNTPPKLAKQSRDRALDI
HHHHHHHHHHHHHCCCHHHCCEEEEEEEHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHH
AHRYGITAATNPGSQAHPKGTGCCSDQEVFDKAGIPVLSVEATNWSLGKKDGYQQRAVSP
HHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCHHHCCCC
HFPQGISWHRPQYDNLQYLERNLPGRIDKRSRESVQILLPLIKELAQAHPPKAPKKK
CCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MFSRFYLKAGLLVAVLGGVFSVSAATQPAKDLPMGKFAADQIRHIATYFPGRMAGSPAEL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHH
LTADYLNQQFSKMGYQSDIRSFNTRYLYTSKDGKKNWNNVTASSVIAAKNGSNPQQILIV
HHHHHHHHHHHHCCHHHHHHHCCCEEEEECCCCCCCCCCCCHHHEEEECCCCCCEEEEEE
AHFDTYTPQSDADLDNNLGGLTLQGVDDNASGIGVMLELAERLKNIPTAYGLRFVATSAE
EEECCCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCHHHHHEEEECCHH
EIGSLGAQNYLQRMSPEEKRNTVLVINLDSLITGDRLYFNAGRNTPPKLAKQSRDRALDI
HHHHHHHHHHHHHCCCHHHCCEEEEEEEHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHH
AHRYGITAATNPGSQAHPKGTGCCSDQEVFDKAGIPVLSVEATNWSLGKKDGYQQRAVSP
HHHCCCEEECCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCHHHCCCC
HFPQGISWHRPQYDNLQYLERNLPGRIDKRSRESVQILLPLIKELAQAHPPKAPKKK
CCCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Aminopeptidases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316184; 9278503; 1316900 [H]