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The map label for this gene is ispH

Identifier: 157368946

GI number: 157368946

Start: 773614

End: 774567

Strand: Direct

Name: ispH

Synonym: Spro_0701

Alternate gene names: 157368946

Gene position: 773614-774567 (Clockwise)

Preceding gene: 157368945

Following gene: 157368948

Centisome position: 14.2

GC content: 58.07

Gene sequence:

>954_bases
ATGCAAATATTGCTGGCTAACCCACGCGGCTTTTGCGCCGGGGTTGATCGTGCGATCAGTATCGTTGAACGCGCGCTGGA
AATTTACGGTGCGCCTATCTACGTGCGTCACGAAGTGGTGCATAACCGCTACGTGGTCGATAGCCTGCGTGAGCGTGGGG
CGGTGTTTATTGAGGAAATCACCGAAGTGCCGGACGGGTCGATTCTGATCTTCTCGGCGCACGGGGTTTCCCAGGCGGTA
AGAGCAGAGGCCAAGGCGCGTGATTTAACCATGCTGTTTGACGCCACCTGCCCGTTGGTGACCAAGGTGCATATGGAGGT
GGCGCGAGCCAGCCGCCGTGGCACCGAGGCGATCCTGATCGGCCACGCCGGTCACCCGGAAGTGGAAGGTACCATGGGCC
AGTACAGCAACCCGAAGGGCGGCATGTACCTGGTGGAGTCACCGGACGACGTCTGGAAGCTGCAGGTGAAGGATGAAAGC
AACCTGTGTTTTATGACGCAAACCACGCTGTCGGTGGACGACACTTCTGACGTGATTGATGCCTTGCGCAAGCGTTTCCC
GAGCATTATCGGGCCGCGTAAGGACGACATCTGCTACGCAACCACCAACCGTCAGGAAGCGGTACGCAACCTGGCCGGGG
ATGCGGACGTGGTGCTGGTGGTGGGGTCGAAAAACTCGTCCAACTCCAACCGTTTGGCCGAATTGGCGCAGCGTGTTGGC
AAACCGGCATACCTGATCGATTCCGCCGCTGACATTCAGGAAGCCTGGCTGAAGGACGCTCATAATATCGGCGTTACCGC
TGGGGCTTCGGCACCTGATGTGTTGGTACAGGATGTTATCAGCCGCCTGAAAGCGCTGGGCGGTGTCAACGTGCAGGAAG
TCAGCGGCCGTGAAGAGAACATCGTGTTCGAAGTACCGAAAGAGCTGCGGGTTGATATCAAACAGGTCGATTAA

Upstream 100 bases:

>100_bases
GAAGATTCGATTACCGTTGATTTCAACCATCCGCTGGCGGGCCATCCGGTCACCTTCGATATTGAAGTGCTGGAGATCGA
TCCGCAGCAGGAGGAGACGC

Downstream 100 bases:

>100_bases
TCAGCATCGGGCATAAATAAGATAACGGGGAGCTTCGGCTCTCCGTTTTGTTTTTAGGGAGCGGTACCCGGCAATAAAAT
TATTAAAAATGAACTTGAGG

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MQILLANPRGFCAGVDRAISIVERALEIYGAPIYVRHEVVHNRYVVDSLRERGAVFIEEITEVPDGSILIFSAHGVSQAV
RAEAKARDLTMLFDATCPLVTKVHMEVARASRRGTEAILIGHAGHPEVEGTMGQYSNPKGGMYLVESPDDVWKLQVKDES
NLCFMTQTTLSVDDTSDVIDALRKRFPSIIGPRKDDICYATTNRQEAVRNLAGDADVVLVVGSKNSSNSNRLAELAQRVG
KPAYLIDSAADIQEAWLKDAHNIGVTAGASAPDVLVQDVISRLKALGGVNVQEVSGREENIVFEVPKELRVDIKQVD

Sequences:

>Translated_317_residues
MQILLANPRGFCAGVDRAISIVERALEIYGAPIYVRHEVVHNRYVVDSLRERGAVFIEEITEVPDGSILIFSAHGVSQAV
RAEAKARDLTMLFDATCPLVTKVHMEVARASRRGTEAILIGHAGHPEVEGTMGQYSNPKGGMYLVESPDDVWKLQVKDES
NLCFMTQTTLSVDDTSDVIDALRKRFPSIIGPRKDDICYATTNRQEAVRNLAGDADVVLVVGSKNSSNSNRLAELAQRVG
KPAYLIDSAADIQEAWLKDAHNIGVTAGASAPDVLVQDVISRLKALGGVNVQEVSGREENIVFEVPKELRVDIKQVD
>Mature_317_residues
MQILLANPRGFCAGVDRAISIVERALEIYGAPIYVRHEVVHNRYVVDSLRERGAVFIEEITEVPDGSILIFSAHGVSQAV
RAEAKARDLTMLFDATCPLVTKVHMEVARASRRGTEAILIGHAGHPEVEGTMGQYSNPKGGMYLVESPDDVWKLQVKDES
NLCFMTQTTLSVDDTSDVIDALRKRFPSIIGPRKDDICYATTNRQEAVRNLAGDADVVLVVGSKNSSNSNRLAELAQRVG
KPAYLIDSAADIQEAWLKDAHNIGVTAGASAPDVLVQDVISRLKALGGVNVQEVSGREENIVFEVPKELRVDIKQVD

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP)

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family

Homologues:

Organism=Escherichia coli, GI1786212, Length=317, Percent_Identity=83.9116719242902, Blast_Score=548, Evalue=1e-157,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPH_SERP5 (A8G9L7)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001476935.1
- STRING:   A8G9L7
- GeneID:   5605464
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0701
- eggNOG:   COG0761
- HOGENOM:   HBG335228
- OMA:   HMQVARA
- ProtClustDB:   PRK01045
- BioCyc:   SPRO399741:SPRO_0701-MONOMER
- HAMAP:   MF_00191
- InterPro:   IPR003451
- TIGRFAMs:   TIGR00216

Pfam domain/function: PF02401 LYTB

EC number: =1.17.1.2

Molecular weight: Translated: 34603; Mature: 34603

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQILLANPRGFCAGVDRAISIVERALEIYGAPIYVRHEVVHNRYVVDSLRERGAVFIEEI
CEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHH
TEVPDGSILIFSAHGVSQAVRAEAKARDLTMLFDATCPLVTKVHMEVARASRRGTEAILI
HCCCCCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEE
GHAGHPEVEGTMGQYSNPKGGMYLVESPDDVWKLQVKDESNLCFMTQTTLSVDDTSDVID
ECCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCEEEEEEEEECCCCHHHHHH
ALRKRFPSIIGPRKDDICYATTNRQEAVRNLAGDADVVLVVGSKNSSNSNRLAELAQRVG
HHHHHCHHHCCCCCCCEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHC
KPAYLIDSAADIQEAWLKDAHNIGVTAGASAPDVLVQDVISRLKALGGVNVQEVSGREEN
CCEEEECCHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCC
IVFEVPKELRVDIKQVD
EEEECCHHHCCHHHCCC
>Mature Secondary Structure
MQILLANPRGFCAGVDRAISIVERALEIYGAPIYVRHEVVHNRYVVDSLRERGAVFIEEI
CEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHH
TEVPDGSILIFSAHGVSQAVRAEAKARDLTMLFDATCPLVTKVHMEVARASRRGTEAILI
HCCCCCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEE
GHAGHPEVEGTMGQYSNPKGGMYLVESPDDVWKLQVKDESNLCFMTQTTLSVDDTSDVID
ECCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEECCCCEEEEEEEEECCCCHHHHHH
ALRKRFPSIIGPRKDDICYATTNRQEAVRNLAGDADVVLVVGSKNSSNSNRLAELAQRVG
HHHHHCHHHCCCCCCCEEEEECCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHC
KPAYLIDSAADIQEAWLKDAHNIGVTAGASAPDVLVQDVISRLKALGGVNVQEVSGREEN
CCEEEECCHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCC
IVFEVPKELRVDIKQVD
EEEECCHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA