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The map label for this gene is prfC [H]

Identifier: 157368897

GI number: 157368897

Start: 716580

End: 718169

Strand: Direct

Name: prfC [H]

Synonym: Spro_0652

Alternate gene names: 157368897

Gene position: 716580-718169 (Clockwise)

Preceding gene: 157368896

Following gene: 157368898

Centisome position: 13.15

GC content: 54.97

Gene sequence:

>1590_bases
ATGTCTCCTAGTGAATTTGCCCGCGAAGTCTCGAAAAGAAGAACTTTCGCCATCATCTCGCACCCCGATGCCGGTAAAAC
CACCATTACCGAAAAGGTGTTGTTATTCGGACAGGCTATCCAGACCGCCGGAACGGTAAAGGGCCGCGGCTCAAATCAGC
ATGCCAAATCCGACTGGATGGAAATGGAAAAGCAGCGTGGGATCTCAATCACCACCTCGGTGATGCAGTTTCCGTACCGC
GAATGTCTGGTTAACCTGCTGGATACCCCGGGGCACGAAGACTTCTCCGAAGATACTTACCGCACCCTGACCGCCGTTGA
CTGCTGTCTGATGGTGATCGACGCCGCCAAAGGGGTTGAGGATCGTACCCGCAAGCTGATGGAAGTTACCCGCCTGCGCG
ATACGCCGATCCTGACCTTTATGAACAAATTGGACCGCGATATCCGCGATCCGATGGAAGTGATGGATGAAGTGGAGCGT
GAGCTGAAAATCGCCTGCTCACCTATCACCTGGCCTATCGGCTGCGGCAAGCTGTTTAAAGGGGTTTATCATCTGTACAA
GGATGAAACCTATCTTTACCAGACCGGTAAAGGCCACACCATTCAGGAAGTGCGCATAGTCAAAGGGTTGGATAACCCGG
AGCTGGACGCCGCCGTGGGTGAAGATCTGGCCGCGCAGCTGCGTGAAGAGCTGGAACTGGTGCAGGGCGCATCTCACGAG
TTCGATCATGAAGCTTTCCTGAGCGGTGATTTGACGCCGGTATTCTTCGGTACCGCTTTGGGTAACTTCGGTGTGGACCA
TATGCTTGACGGTCTGGTGGCCTGGGCTCCAGCGCCGATGCCGCGTCAAAGCAGCACCCGTGAAGTGGTAGCCGCAGAAG
AGAAATTCAGCGGTTTCGTGTTCAAGATCCAGGCCAATATGGATCCTAAACACCGTGACCGCGTGGCCTTTATGCGTGTG
GTCTCCGGACGCTACGAGAAAGGCATGAAGCTGCGCCAGGTGCGTACCGGTAAAGACGTGGTGATCTCCGACGCCCTGAC
CTTTATGGCGGGTGACCGTTCGCACGTTGAAGAGGCTTATCCGGGCGATATTATTGGTCTGCATAACCACGGCACCATTC
AGATCGGCGATACCTTCACCCAGGGTGAAGATATGAAGTTCACCGGTATTCCGAACTTCGCGCCGGAGCTGTTCCGTCGC
ATTCGTCTGCGCGATCCGTTGAAGCAAAAACAGCTGCTGAAAGGCCTGGTGCAACTGTCCGAAGAAGGGGCGGTGCAGGT
ATTCCGTCCGATCGCCAGCAACGATTTGATCGTCGGCGCGGTCGGTGTGCTGCAGTTCGAAGTGGTGGTTGCACGCCTGA
AAAGCGAATACAACGTCGAAGCCTTGTACGAGTCGGTCAACGTTTCGACCGCCCGTTGGGTGGAATGCAGCGACGTGAAG
AAGTTTGAAGAGTTCAAGCGCAAGTGTGAGCTTAACCTGGCATTGGACGGCGGCGACAACCTGTCTTACATCGCGCCAAC
CATGGTTAATCTGAATATCACCCAGGAACGCTACCCTGAAGTGGTGTTCCGCAAAACACGCGAGCACTAA

Upstream 100 bases:

>100_bases
AATGCCCGGCTATTATCTTTTATTTACCGCTTTGCCTGGCGCCGTGCGCGGCGCGTTCTGATGCTTTCGCAGGGCGGACT
AACTGTAGAATCTGAAAAAC

Downstream 100 bases:

>100_bases
CGGTAACAGAACAGCAGGGCGCCTTAGGGCGCCCTGTGTTATATTGCTTCACCCCATTCTGCTAATTAATAAGTCTCCTT
CATCTTTTCAGTCCCCCCGC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSPSEFAREVSKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYR
ECLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMEVMDEVER
ELKIACSPITWPIGCGKLFKGVYHLYKDETYLYQTGKGHTIQEVRIVKGLDNPELDAAVGEDLAAQLREELELVQGASHE
FDHEAFLSGDLTPVFFGTALGNFGVDHMLDGLVAWAPAPMPRQSSTREVVAAEEKFSGFVFKIQANMDPKHRDRVAFMRV
VSGRYEKGMKLRQVRTGKDVVISDALTFMAGDRSHVEEAYPGDIIGLHNHGTIQIGDTFTQGEDMKFTGIPNFAPELFRR
IRLRDPLKQKQLLKGLVQLSEEGAVQVFRPIASNDLIVGAVGVLQFEVVVARLKSEYNVEALYESVNVSTARWVECSDVK
KFEEFKRKCELNLALDGGDNLSYIAPTMVNLNITQERYPEVVFRKTREH

Sequences:

>Translated_529_residues
MSPSEFAREVSKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYR
ECLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMEVMDEVER
ELKIACSPITWPIGCGKLFKGVYHLYKDETYLYQTGKGHTIQEVRIVKGLDNPELDAAVGEDLAAQLREELELVQGASHE
FDHEAFLSGDLTPVFFGTALGNFGVDHMLDGLVAWAPAPMPRQSSTREVVAAEEKFSGFVFKIQANMDPKHRDRVAFMRV
VSGRYEKGMKLRQVRTGKDVVISDALTFMAGDRSHVEEAYPGDIIGLHNHGTIQIGDTFTQGEDMKFTGIPNFAPELFRR
IRLRDPLKQKQLLKGLVQLSEEGAVQVFRPIASNDLIVGAVGVLQFEVVVARLKSEYNVEALYESVNVSTARWVECSDVK
KFEEFKRKCELNLALDGGDNLSYIAPTMVNLNITQERYPEVVFRKTREH
>Mature_528_residues
SPSEFAREVSKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYRE
CLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMEVMDEVERE
LKIACSPITWPIGCGKLFKGVYHLYKDETYLYQTGKGHTIQEVRIVKGLDNPELDAAVGEDLAAQLREELELVQGASHEF
DHEAFLSGDLTPVFFGTALGNFGVDHMLDGLVAWAPAPMPRQSSTREVVAAEEKFSGFVFKIQANMDPKHRDRVAFMRVV
SGRYEKGMKLRQVRTGKDVVISDALTFMAGDRSHVEEAYPGDIIGLHNHGTIQIGDTFTQGEDMKFTGIPNFAPELFRRI
RLRDPLKQKQLLKGLVQLSEEGAVQVFRPIASNDLIVGAVGVLQFEVVVARLKSEYNVEALYESVNVSTARWVECSDVKK
FEEFKRKCELNLALDGGDNLSYIAPTMVNLNITQERYPEVVFRKTREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=491, Percent_Identity=27.0875763747454, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI19923640, Length=510, Percent_Identity=24.9019607843137, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI25306283, Length=394, Percent_Identity=26.6497461928934, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI25306287, Length=482, Percent_Identity=25.103734439834, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI157426893, Length=172, Percent_Identity=29.0697674418605, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI94966754, Length=148, Percent_Identity=29.0540540540541, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI310132016, Length=125, Percent_Identity=29.6, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI310110807, Length=125, Percent_Identity=29.6, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI310123363, Length=125, Percent_Identity=29.6, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1790835, Length=529, Percent_Identity=88.8468809073724, Blast_Score=994, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=494, Percent_Identity=26.1133603238866, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI48994988, Length=198, Percent_Identity=31.3131313131313, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788922, Length=151, Percent_Identity=33.112582781457, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17533571, Length=485, Percent_Identity=26.8041237113402, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17556745, Length=179, Percent_Identity=31.2849162011173, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=160, Percent_Identity=31.25, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=496, Percent_Identity=27.4193548387097, Blast_Score=176, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6322359, Length=417, Percent_Identity=26.6187050359712, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=25.1655629139073, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324707, Length=155, Percent_Identity=27.0967741935484, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320593, Length=155, Percent_Identity=27.0967741935484, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582462, Length=478, Percent_Identity=26.3598326359833, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI221458488, Length=505, Percent_Identity=24.950495049505, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI78706572, Length=165, Percent_Identity=32.7272727272727, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=29.8701298701299, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=29.8701298701299, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=29.8701298701299, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59622; Mature: 59491

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPSEFAREVSKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWM
CCHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
EMEKQRGISITTSVMQFPYRECLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVE
HHHHHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
DRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMEVMDEVERELKIACSPITWPIGCGKLFK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEECCCCCCCCHHHHHH
GVYHLYKDETYLYQTGKGHTIQEVRIVKGLDNPELDAAVGEDLAAQLREELELVQGASHE
HHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
FDHEAFLSGDLTPVFFGTALGNFGVDHMLDGLVAWAPAPMPRQSSTREVVAAEEKFSGFV
CCCHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEE
FKIQANMDPKHRDRVAFMRVVSGRYEKGMKLRQVRTGKDVVISDALTFMAGDRSHVEEAY
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEHHHHHHHCCCHHHHHHCC
PGDIIGLHNHGTIQIGDTFTQGEDMKFTGIPNFAPELFRRIRLRDPLKQKQLLKGLVQLS
CCCEEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHC
EEGAVQVFRPIASNDLIVGAVGVLQFEVVVARLKSEYNVEALYESVNVSTARWVECSDVK
CCCCHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEHHHHH
KFEEFKRKCELNLALDGGDNLSYIAPTMVNLNITQERYPEVVFRKTREH
HHHHHHHHCEEEEEEECCCCCCEECCEEEEEEECHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SPSEFAREVSKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGTVKGRGSNQHAKSDWM
CHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
EMEKQRGISITTSVMQFPYRECLVNLLDTPGHEDFSEDTYRTLTAVDCCLMVIDAAKGVE
HHHHHCCCEEEHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
DRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMEVMDEVERELKIACSPITWPIGCGKLFK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHEEECCCCCCCCHHHHHH
GVYHLYKDETYLYQTGKGHTIQEVRIVKGLDNPELDAAVGEDLAAQLREELELVQGASHE
HHHHHHCCCEEEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
FDHEAFLSGDLTPVFFGTALGNFGVDHMLDGLVAWAPAPMPRQSSTREVVAAEEKFSGFV
CCCHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCEE
FKIQANMDPKHRDRVAFMRVVSGRYEKGMKLRQVRTGKDVVISDALTFMAGDRSHVEEAY
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCCEEEHHHHHHHCCCHHHHHHCC
PGDIIGLHNHGTIQIGDTFTQGEDMKFTGIPNFAPELFRRIRLRDPLKQKQLLKGLVQLS
CCCEEEECCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHC
EEGAVQVFRPIASNDLIVGAVGVLQFEVVVARLKSEYNVEALYESVNVSTARWVECSDVK
CCCCHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEHHHHH
KFEEFKRKCELNLALDGGDNLSYIAPTMVNLNITQERYPEVVFRKTREH
HHHHHHHHCEEEEEEECCCCCCEECCEEEEEEECHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA