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The map label for this gene is hpcH [H]

Identifier: 157368876

GI number: 157368876

Start: 696210

End: 697004

Strand: Direct

Name: hpcH [H]

Synonym: Spro_0631

Alternate gene names: 157368876

Gene position: 696210-697004 (Clockwise)

Preceding gene: 157368875

Following gene: 157368877

Centisome position: 12.78

GC content: 61.76

Gene sequence:

>795_bases
ATGTTAACCAACGCTTTCAAGCAGGCGCTGCAGGAAAAACGCCCGCAGATCGGCCTGTGGCTGGGGCTATGCAGTAGCTA
CAGTGCAGAACTGTTGGCCGGCTCCGGCTTTGACTGGCTGCTGATCGACGGCGAGCATGCGCCCAACAATGTCCAAACGG
TGCTGGGCCAGTTGCAGGCGATCGCGCCTTATCCGAGCCAACCGGTGGTACGCCCGGCGTGGAACGACGCGGTGATGATT
AAGCAACTGTTGGACGTCGGTGCCCAGACGCTGCTGATCCCAATGATTCAAAACGCCGAACAGGCGCGTGAAGCGGTGCG
TGCCACCCGTTATCCGCCGCATGGGATACGCGGAGTCGGCAGTGCATTGGCGCGTGCGTCGCGCTGGAATAGGGTACCGG
ATTATCTGCAACAGGCCGATGCGCAGATGTGCGTATTGGTGCAGATCGAAACCCGCGAGGCGGTGAAAAACCTGGATGAG
ATCCTGCAGGTGGAAGGCGTCGACGGGGTGTTTATCGGCCCGGCGGATCTCAGTGCCGACATGGGTTATGCCGGTAATCC
GCAACAGCCGGAGGTGCAACGCACCATCGACGATGCCATTGCGCGCATCAGCGCTGCCGGCAAGGCACCGGGCATTCTGA
CCGCCAATCCTGAGCTGGCGCAGCATTACCTGGCGTCCGGCGCGCTGTTTGTCGCGGTAGGGGTGGATACCACCCTGTTG
GCGCGGGCGGCGGAGTCCCTGGCCGGTCGTTTTAAGCAGCAACAGCCTCCCGAGCAATCCCCGGGCGTTTATTAG

Upstream 100 bases:

>100_bases
TTTACCCCCTTTCCATTGGGAGAGGGGATCGATCGAGCCGGTTTACTCCCTCTCCTTTGGGAGAGGGCCGGGGTGAGGGG
AAAACACCAAGGAGAACATC

Downstream 100 bases:

>100_bases
GTTGCAGGACGCAATCTTACTGGAGACACCATGAGCAACGTCGATTCGCTGCCGCCGGTCAACCCGGCGCAGCAACATAA
AGCGTTAACCGCCACCGAAC

Product: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MLTNAFKQALQEKRPQIGLWLGLCSSYSAELLAGSGFDWLLIDGEHAPNNVQTVLGQLQAIAPYPSQPVVRPAWNDAVMI
KQLLDVGAQTLLIPMIQNAEQAREAVRATRYPPHGIRGVGSALARASRWNRVPDYLQQADAQMCVLVQIETREAVKNLDE
ILQVEGVDGVFIGPADLSADMGYAGNPQQPEVQRTIDDAIARISAAGKAPGILTANPELAQHYLASGALFVAVGVDTTLL
ARAAESLAGRFKQQQPPEQSPGVY

Sequences:

>Translated_264_residues
MLTNAFKQALQEKRPQIGLWLGLCSSYSAELLAGSGFDWLLIDGEHAPNNVQTVLGQLQAIAPYPSQPVVRPAWNDAVMI
KQLLDVGAQTLLIPMIQNAEQAREAVRATRYPPHGIRGVGSALARASRWNRVPDYLQQADAQMCVLVQIETREAVKNLDE
ILQVEGVDGVFIGPADLSADMGYAGNPQQPEVQRTIDDAIARISAAGKAPGILTANPELAQHYLASGALFVAVGVDTTLL
ARAAESLAGRFKQQQPPEQSPGVY
>Mature_264_residues
MLTNAFKQALQEKRPQIGLWLGLCSSYSAELLAGSGFDWLLIDGEHAPNNVQTVLGQLQAIAPYPSQPVVRPAWNDAVMI
KQLLDVGAQTLLIPMIQNAEQAREAVRATRYPPHGIRGVGSALARASRWNRVPDYLQQADAQMCVLVQIETREAVKNLDE
ILQVEGVDGVFIGPADLSADMGYAGNPQQPEVQRTIDDAIARISAAGKAPGILTANPELAQHYLASGALFVAVGVDTTLL
ARAAESLAGRFKQQQPPEQSPGVY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=264, Percent_Identity=56.4393939393939, Blast_Score=306, Evalue=8e-85,
Organism=Escherichia coli, GI1789514, Length=253, Percent_Identity=46.6403162055336, Blast_Score=229, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 28414; Mature: 28414

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTNAFKQALQEKRPQIGLWLGLCSSYSAELLAGSGFDWLLIDGEHAPNNVQTVLGQLQA
CCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH
IAPYPSQPVVRPAWNDAVMIKQLLDVGAQTLLIPMIQNAEQAREAVRATRYPPHGIRGVG
HCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHH
SALARASRWNRVPDYLQQADAQMCVLVQIETREAVKNLDEILQVEGVDGVFIGPADLSAD
HHHHHHHHCCCCHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
MGYAGNPQQPEVQRTIDDAIARISAAGKAPGILTANPELAQHYLASGALFVAVGVDTTLL
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHCCCEEEEECCCHHHH
ARAAESLAGRFKQQQPPEQSPGVY
HHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLTNAFKQALQEKRPQIGLWLGLCSSYSAELLAGSGFDWLLIDGEHAPNNVQTVLGQLQA
CCCHHHHHHHHHCCCCHHHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHH
IAPYPSQPVVRPAWNDAVMIKQLLDVGAQTLLIPMIQNAEQAREAVRATRYPPHGIRGVG
HCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHH
SALARASRWNRVPDYLQQADAQMCVLVQIETREAVKNLDEILQVEGVDGVFIGPADLSAD
HHHHHHHHCCCCHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
MGYAGNPQQPEVQRTIDDAIARISAAGKAPGILTANPELAQHYLASGALFVAVGVDTTLL
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHCCCEEEEECCCHHHH
ARAAESLAGRFKQQQPPEQSPGVY
HHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA