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The map label for this gene is cls [C]

Identifier: 157368849

GI number: 157368849

Start: 669282

End: 670532

Strand: Direct

Name: cls [C]

Synonym: Spro_0604

Alternate gene names: 157368849

Gene position: 669282-670532 (Clockwise)

Preceding gene: 157368848

Following gene: 157368853

Centisome position: 12.28

GC content: 59.39

Gene sequence:

>1251_bases
ATGCCTAATGGCCGTCAACCACGGCGGCCGCTGGGCCGCCTTTACACTGCGTTATGGGGTAGCTGCCTGTTGATGTTAAG
CCAGAGTGCGACGGCGCGTTTTGATATTCCCGGCTACGAGCTGGTGTATACCGCGCCGGTGGAAACCGCGTTGCAGGCGG
ATGACCTGCGTAACACCGCGCAAGTGTGGCAGCAGATGTTCGATGCGGCGAAAACCCGCATCGATCTGGGCCAGTTCTAC
GTGGCGAACCAACAGGGTTCGCTGCTGGATGGCGTGCTGCAACACCTGAAAGCGGCGGGCGAACGCGGAGTGAAAATTCG
TTTTCTGATGGAAGAGAAGGGCATCCGCATCTCTACCCCTGAGACGCTGGAGCAGCTTAAGGCGATCCCTAACCTGGAGC
TGCGTATTATTCCTTATCAGCGGCTGAGCGGCGGTATTTTGCATGCCAAATACCTGCTGGTGGACGGTGAGCAGGCGTTT
GTCGGCAGCCAGAACTTCGACTGGCGTGCGTTGGCGCATATCCATGAAACCGGGCTGCGCATCAGCGATGCCGGGGTGGT
CGGGCAGATCCAGGCGATCTTCGAGCAGGATTGGCAAGCTCAGGCTTTGCTGGCGGCGGATAAGCCGGTGCCGCAACGGC
CTTACCAGCCGAGCACCGAAACGCCGCAGGGTAACTATTTGGTCGCCAGTCCGCGTGCCTATAATCCGGCCGGGGTGATT
GACTCGCAGGTGGAGTTGCCGCGTCTGCTGGCTACGGCCATACAGCGAGTACGGGTGCAGGTGATGGATTACGCGCCATT
GTCTTTTGGCCCAGACCACACCCGGCCTTATTACGCGACGATCGACAATGCCCTGCGCAGCGCTGCCGCCCGCGGCGTGC
AGGTTGAGCTGATGGTCGCCAACTGGAACACCAAAAAACCGGATATCGCCTGGCTGAAAAGTCTGGCGGTGGTGCCGAAC
GTGCAGATAAAGATCGTCACTATTCCCCCGGCCAGCAGCGGCTTTATCCCGTTCGCCCGGGTGATCCACAGCAAAATCAT
GACCATTGACGGTGAAACGGCGTGGATCGGCACCAGCAACTGGACCGGCGGTTATCTGGATAACTCGCGCAATCTGGAGT
TGGTACTGCACAATCCGGCCATGAGCCAGCGGGTGGATAAGCTCTACAGCCAGCTATGGGACAGCGTTTACGCCGAACCG
ATCAAGCTCGATTATGACTACCCGGCCCCCAAACCGGGTGGTGAGTCCTGA

Upstream 100 bases:

>100_bases
AACGAGCTGCTCAAAGTGCTGCTGATCCGCGCCGGTGCGCCAGCCAGCCGCCCGGAAAACGGCAACCGCGAGCAATGGGT
TGACAGTGAGGCACCGCGCT

Downstream 100 bases:

>100_bases
CTGATACAGGGCGCGGCATGCCGCGCCTCTACTCTTATGCTCGCCAGCCGGCTTGTTCCAGCGCGGTCAGCAACTGCCCG
GCACTTAGTCCTTCTGCCCG

Product: phospholipase D/transphosphatidylase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: Phospholipase D/Transphosphatidylase; Phospholipase D/ Transphosphatidylase; Cardiolipin Synthase; Phospholipase D; Phospholipase

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MPNGRQPRRPLGRLYTALWGSCLLMLSQSATARFDIPGYELVYTAPVETALQADDLRNTAQVWQQMFDAAKTRIDLGQFY
VANQQGSLLDGVLQHLKAAGERGVKIRFLMEEKGIRISTPETLEQLKAIPNLELRIIPYQRLSGGILHAKYLLVDGEQAF
VGSQNFDWRALAHIHETGLRISDAGVVGQIQAIFEQDWQAQALLAADKPVPQRPYQPSTETPQGNYLVASPRAYNPAGVI
DSQVELPRLLATAIQRVRVQVMDYAPLSFGPDHTRPYYATIDNALRSAAARGVQVELMVANWNTKKPDIAWLKSLAVVPN
VQIKIVTIPPASSGFIPFARVIHSKIMTIDGETAWIGTSNWTGGYLDNSRNLELVLHNPAMSQRVDKLYSQLWDSVYAEP
IKLDYDYPAPKPGGES

Sequences:

>Translated_416_residues
MPNGRQPRRPLGRLYTALWGSCLLMLSQSATARFDIPGYELVYTAPVETALQADDLRNTAQVWQQMFDAAKTRIDLGQFY
VANQQGSLLDGVLQHLKAAGERGVKIRFLMEEKGIRISTPETLEQLKAIPNLELRIIPYQRLSGGILHAKYLLVDGEQAF
VGSQNFDWRALAHIHETGLRISDAGVVGQIQAIFEQDWQAQALLAADKPVPQRPYQPSTETPQGNYLVASPRAYNPAGVI
DSQVELPRLLATAIQRVRVQVMDYAPLSFGPDHTRPYYATIDNALRSAAARGVQVELMVANWNTKKPDIAWLKSLAVVPN
VQIKIVTIPPASSGFIPFARVIHSKIMTIDGETAWIGTSNWTGGYLDNSRNLELVLHNPAMSQRVDKLYSQLWDSVYAEP
IKLDYDYPAPKPGGES
>Mature_415_residues
PNGRQPRRPLGRLYTALWGSCLLMLSQSATARFDIPGYELVYTAPVETALQADDLRNTAQVWQQMFDAAKTRIDLGQFYV
ANQQGSLLDGVLQHLKAAGERGVKIRFLMEEKGIRISTPETLEQLKAIPNLELRIIPYQRLSGGILHAKYLLVDGEQAFV
GSQNFDWRALAHIHETGLRISDAGVVGQIQAIFEQDWQAQALLAADKPVPQRPYQPSTETPQGNYLVASPRAYNPAGVID
SQVELPRLLATAIQRVRVQVMDYAPLSFGPDHTRPYYATIDNALRSAAARGVQVELMVANWNTKKPDIAWLKSLAVVPNV
QIKIVTIPPASSGFIPFARVIHSKIMTIDGETAWIGTSNWTGGYLDNSRNLELVLHNPAMSQRVDKLYSQLWDSVYAEPI
KLDYDYPAPKPGGES

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI166197683, Length=413, Percent_Identity=27.3607748184019, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI72534684, Length=413, Percent_Identity=27.3607748184019, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI94681038, Length=390, Percent_Identity=28.974358974359, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17559458, Length=395, Percent_Identity=27.5949367088608, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI193207667, Length=395, Percent_Identity=27.5949367088608, Blast_Score=164, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI193208604, Length=405, Percent_Identity=24.6913580246914, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17565994, Length=412, Percent_Identity=24.7572815533981, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI32566758, Length=412, Percent_Identity=24.7572815533981, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17532785, Length=373, Percent_Identity=23.3243967828418, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585591, Length=391, Percent_Identity=29.156010230179, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI20129681, Length=391, Percent_Identity=29.156010230179, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.8.- [C]

Molecular weight: Translated: 46242; Mature: 46111

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNGRQPRRPLGRLYTALWGSCLLMLSQSATARFDIPGYELVYTAPVETALQADDLRNTA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCHHHHHHHHHHHHHH
QVWQQMFDAAKTRIDLGQFYVANQQGSLLDGVLQHLKAAGERGVKIRFLMEEKGIRISTP
HHHHHHHHHHHHHEECCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCEECCH
ETLEQLKAIPNLELRIIPYQRLSGGILHAKYLLVDGEQAFVGSQNFDWRALAHIHETGLR
HHHHHHHCCCCCEEEEEEHHHHCCCEEEEEEEEEECCEEEECCCCCCHHHHHHHHHCCCE
ISDAGVVGQIQAIFEQDWQAQALLAADKPVPQRPYQPSTETPQGNYLVASPRAYNPAGVI
EECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
DSQVELPRLLATAIQRVRVQVMDYAPLSFGPDHTRPYYATIDNALRSAAARGVQVELMVA
CCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEE
NWNTKKPDIAWLKSLAVVPNVQIKIVTIPPASSGFIPFARVIHSKIMTIDGETAWIGTSN
CCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHEEECCCEEEEECCC
WTGGYLDNSRNLELVLHNPAMSQRVDKLYSQLWDSVYAEPIKLDYDYPAPKPGGES
CCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure 
PNGRQPRRPLGRLYTALWGSCLLMLSQSATARFDIPGYELVYTAPVETALQADDLRNTA
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCHHHHHHHHHHHHHH
QVWQQMFDAAKTRIDLGQFYVANQQGSLLDGVLQHLKAAGERGVKIRFLMEEKGIRISTP
HHHHHHHHHHHHHEECCCEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCEECCH
ETLEQLKAIPNLELRIIPYQRLSGGILHAKYLLVDGEQAFVGSQNFDWRALAHIHETGLR
HHHHHHHCCCCCEEEEEEHHHHCCCEEEEEEEEEECCEEEECCCCCCHHHHHHHHHCCCE
ISDAGVVGQIQAIFEQDWQAQALLAADKPVPQRPYQPSTETPQGNYLVASPRAYNPAGVI
EECCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCC
DSQVELPRLLATAIQRVRVQVMDYAPLSFGPDHTRPYYATIDNALRSAAARGVQVELMVA
CCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEE
NWNTKKPDIAWLKSLAVVPNVQIKIVTIPPASSGFIPFARVIHSKIMTIDGETAWIGTSN
CCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHEEECCCEEEEECCC
WTGGYLDNSRNLELVLHNPAMSQRVDKLYSQLWDSVYAEPIKLDYDYPAPKPGGES
CCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA