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The map label for this gene is tnpR [H]

Identifier: 157368628

GI number: 157368628

Start: 426993

End: 427436

Strand: Reverse

Name: tnpR [H]

Synonym: Spro_0381

Alternate gene names: 157368628

Gene position: 427436-426993 (Counterclockwise)

Preceding gene: 157368632

Following gene: 157368627

Centisome position: 7.84

GC content: 59.46

Gene sequence:

>444_bases
ATGCATAAAACGACCGTTTTATGGACTGGACTGACCCCGCCCCGGTGTTCGACCGATAAACAGGACTTAACCGCGCAACA
GGAAGCGCTGATGAAACTCGGTGTCTCCCCGGACCGTATTTACCTCGATAAAGGGCTGACGGGGTCAAACCGGCAGCGGC
CCGGCCTGGACCAGGCGCTGGCCGCCGTTCGCAGTGGCGACACCCTGGTGGTACCGAAACTCGACCGCCTGGCCCGTTCA
GTCCCTGATGCACGTCAAATCGCCGACGCCCTGCAGGTCAGAGGCGTGAAGCTGGCACTGGGTACTAGCCTCTATGATCC
GGCCGACCCGATGGGGAAAATGTTTTTCAACGTGCTGGCCACGTTTGCCGAGTTTGAAGGCGATTTGATACGCCTGCGCA
CCCGCGAAGGGAAAACTGCGGGGAAAACAGCCAAAGCTGTCTGA

Upstream 100 bases:

>100_bases
CATGGTGTTCTCCTTTTTTAACGTTCAGATGAGAATAACCGATCTTTTAATGGTTCTAATTAAATCTAATTTAAATTGTG
GGGTTAATAAAAACTATCAC

Downstream 100 bases:

>100_bases
AAAACAACAAAAAGAGCTGTGCCGCATGCGAGAAACCGGCCAGTACTCGATCAGCTATCTGACGGAGTTATTCGCGGTAT
CCAGACCCACCGTTTACCGC

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 147; Mature: 147

Protein sequence:

>147_residues
MHKTTVLWTGLTPPRCSTDKQDLTAQQEALMKLGVSPDRIYLDKGLTGSNRQRPGLDQALAAVRSGDTLVVPKLDRLARS
VPDARQIADALQVRGVKLALGTSLYDPADPMGKMFFNVLATFAEFEGDLIRLRTREGKTAGKTAKAV

Sequences:

>Translated_147_residues
MHKTTVLWTGLTPPRCSTDKQDLTAQQEALMKLGVSPDRIYLDKGLTGSNRQRPGLDQALAAVRSGDTLVVPKLDRLARS
VPDARQIADALQVRGVKLALGTSLYDPADPMGKMFFNVLATFAEFEGDLIRLRTREGKTAGKTAKAV
>Mature_147_residues
MHKTTVLWTGLTPPRCSTDKQDLTAQQEALMKLGVSPDRIYLDKGLTGSNRQRPGLDQALAAVRSGDTLVVPKLDRLARS
VPDARQIADALQVRGVKLALGTSLYDPADPMGKMFFNVLATFAEFEGDLIRLRTREGKTAGKTAKAV

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=123, Percent_Identity=39.8373983739837, Blast_Score=82, Evalue=1e-17,
Organism=Escherichia coli, GI1787638, Length=130, Percent_Identity=36.1538461538462, Blast_Score=68, Evalue=2e-13,
Organism=Escherichia coli, GI1787827, Length=130, Percent_Identity=36.1538461538462, Blast_Score=68, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 15980; Mature: 15980

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKTTVLWTGLTPPRCSTDKQDLTAQQEALMKLGVSPDRIYLDKGLTGSNRQRPGLDQAL
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHH
AAVRSGDTLVVPKLDRLARSVPDARQIADALQVRGVKLALGTSLYDPADPMGKMFFNVLA
HHHHCCCEEECCCHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHH
TFAEFEGDLIRLRTREGKTAGKTAKAV
HHHHCCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MHKTTVLWTGLTPPRCSTDKQDLTAQQEALMKLGVSPDRIYLDKGLTGSNRQRPGLDQAL
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHH
AAVRSGDTLVVPKLDRLARSVPDARQIADALQVRGVKLALGTSLYDPADPMGKMFFNVLA
HHHHCCCEEECCCHHHHHHCCCHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHH
TFAEFEGDLIRLRTREGKTAGKTAKAV
HHHHCCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2170815; 2555186 [H]