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The map label for this gene is rhtC [H]

Identifier: 157368443

GI number: 157368443

Start: 218796

End: 219422

Strand: Direct

Name: rhtC [H]

Synonym: Spro_0194

Alternate gene names: 157368443

Gene position: 218796-219422 (Clockwise)

Preceding gene: 157368442

Following gene: 157368445

Centisome position: 4.02

GC content: 58.53

Gene sequence:

>627_bases
ATGCTGATGCTGTTTCTGACCGTGGCGCTGGTGCACCTGATTGCGCTGATGAGCCCGGGGCCGGACTTCTTTTTTGTTTC
GCAAACCGCCGCCAGCCGCTCGCGCCGTGAGGCGATGATGGGCGTGCTGGGCATTTCACTGGGTATCGTAGTGTGGGCGG
GCGTAGCCTTGATGGGCCTGCATCTGATCCTGCAAAAAATGGCCTGGCTGCACCAAATTATCATGGTTGGCGGTGGGATT
TACCTGTGCTGGATGGGCTGGCAACTGTTGCGCTCCGCCCGTTCTCAAAATGCCCAGTCGGTGCCTGCGGCGGAGATGAA
AGTGGCCTTGCCGAAGCCGGGGCGCAGCTTTATCCGCGGTTTTCTGACCAACCTGTCCAACCCGAAGGCGGTTATCTATT
TCGGCAGCGTGTTTTCACTGTTTGTGGGTGACGACGTCGGGGCCGGTGCACGCTGGGGCTTGTTCTTGCTGATCATCGCC
GAGACTTTTGTCTGGTTCAGCCTGGTGGCGGTGGTGTTTGCATTGCCGACCATGCGCCGGGGTTATCAACGGCTGGCCAA
GTGGATAGACGGACTGGCGGGCGTGCTGTTTACCGGTTTTGGTTTACACCTGATCTTTACCCGCTAA

Upstream 100 bases:

>100_bases
TAACGGTGTCGGTCAGCGCAAGCTGGAGCGTTTTGGCGCGCCGTTTATGGCGATGATCCGCGATCACCTCGATAATGACG
AAGATTAAGGGCAATAAATC

Downstream 100 bases:

>100_bases
ACACTGCCGCACAGATTTTGAGGGCCGAACTGCCATGTTCGGCCCTTTTGCATTATGCCTTTCTGGCCGTAGCCAGCAGC
CCGGCCACCAGAATAAACAG

Product: threonine efflux system

Products: Proton [Cytoplasm]; L-threonine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MLMLFLTVALVHLIALMSPGPDFFFVSQTAASRSRREAMMGVLGISLGIVVWAGVALMGLHLILQKMAWLHQIIMVGGGI
YLCWMGWQLLRSARSQNAQSVPAAEMKVALPKPGRSFIRGFLTNLSNPKAVIYFGSVFSLFVGDDVGAGARWGLFLLIIA
ETFVWFSLVAVVFALPTMRRGYQRLAKWIDGLAGVLFTGFGLHLIFTR

Sequences:

>Translated_208_residues
MLMLFLTVALVHLIALMSPGPDFFFVSQTAASRSRREAMMGVLGISLGIVVWAGVALMGLHLILQKMAWLHQIIMVGGGI
YLCWMGWQLLRSARSQNAQSVPAAEMKVALPKPGRSFIRGFLTNLSNPKAVIYFGSVFSLFVGDDVGAGARWGLFLLIIA
ETFVWFSLVAVVFALPTMRRGYQRLAKWIDGLAGVLFTGFGLHLIFTR
>Mature_208_residues
MLMLFLTVALVHLIALMSPGPDFFFVSQTAASRSRREAMMGVLGISLGIVVWAGVALMGLHLILQKMAWLHQIIMVGGGI
YLCWMGWQLLRSARSQNAQSVPAAEMKVALPKPGRSFIRGFLTNLSNPKAVIYFGSVFSLFVGDDVGAGARWGLFLLIIA
ETFVWFSLVAVVFALPTMRRGYQRLAKWIDGLAGVLFTGFGLHLIFTR

Specific function: Conducts the efflux of threonine [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994978, Length=208, Percent_Identity=72.5961538461538, Blast_Score=320, Evalue=5e-89,
Organism=Escherichia coli, GI1786522, Length=205, Percent_Identity=30.2439024390244, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788099, Length=196, Percent_Identity=27.5510204081633, Blast_Score=67, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22880; Mature: 22880

Theoretical pI: Translated: 11.46; Mature: 11.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMLFLTVALVHLIALMSPGPDFFFVSQTAASRSRREAMMGVLGISLGIVVWAGVALMGL
CHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLILQKMAWLHQIIMVGGGIYLCWMGWQLLRSARSQNAQSVPAAEMKVALPKPGRSFIRG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHEEECCCCCHHHHHH
FLTNLSNPKAVIYFGSVFSLFVGDDVGAGARWGLFLLIIAETFVWFSLVAVVFALPTMRR
HHHCCCCCCEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYQRLAKWIDGLAGVLFTGFGLHLIFTR
HHHHHHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MLMLFLTVALVHLIALMSPGPDFFFVSQTAASRSRREAMMGVLGISLGIVVWAGVALMGL
CHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLILQKMAWLHQIIMVGGGIYLCWMGWQLLRSARSQNAQSVPAAEMKVALPKPGRSFIRG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHEEECCCCCHHHHHH
FLTNLSNPKAVIYFGSVFSLFVGDDVGAGARWGLFLLIIAETFVWFSLVAVVFALPTMRR
HHHCCCCCCEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GYQRLAKWIDGLAGVLFTGFGLHLIFTR
HHHHHHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-threonine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + L-threonine [Cytoplasm] = Proton [Cytoplasm] + L-threonine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]